BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0005.0268.p (247 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313110|ref|YP_003777202.1| 3-oxoacyl-(acyl-carrier-protein... 114 6e-27 gi|300311790|ref|YP_003775882.1| short-chain dehydrogenase/reduc... 111 4e-26 gi|300310426|ref|YP_003774518.1| gluconate 5-dehydrogenase oxido... 98 5e-22 gi|300311568|ref|YP_003775660.1| toluenesulfonate Zinc-independe... 98 6e-22 gi|300313682|ref|YP_003777774.1| short-chain alcohol dehydrogena... 94 7e-21 >gi|300313110|ref|YP_003777202.1| 3-oxoacyl-(acyl-carrier-protein) reductase [Herbaspirillum seropedicae SmR1] Length = 256 Score = 114 bits (286), Expect = 6e-27, Method: Compositional matrix adjust. Identities = 93/255 (36%), Positives = 129/255 (50%), Gaps = 23/255 (9%) Query: 2 NSELRVALVTGSTSGIGLALAHGFAEAGYTVVV---HSRSSVETGEKLAAALPAALYIQA 58 +S RVALVTG GIG +++ A G+TV+V + ++ +T + L AA A +Q Sbjct: 5 DSGARVALVTGGAMGIGASISQQLAAKGHTVLVADINLDAASKTADALVAAGHRAAPLQM 64 Query: 59 DLAVEADRARLIAQILAHYGRLDVLVNNAGISRVIPHGDLSAASPEIWHELYEVNVVAPF 118 DL A A I YGR DVLVNNAG++R D E W + VNV Sbjct: 65 DLGSPESIAAAFAHIEKDYGRCDVLVNNAGVARTYSFLDYPL---ENWLQTMNVNVTG-- 119 Query: 119 RLIALAQEALKNSAKSGTPGCVINISSHAGVRPKGASIPYAATKAALNHMTKLLALSLAP 178 ++ Q A + K G ++NISS +G+R Y +KAA+ +T+ +A+ LA Sbjct: 120 -VLLAGQHAARLMVKQ-QWGRIVNISSISGIRAGAGRTAYGTSKAAVIGLTRQMAIELAQ 177 Query: 179 -DIRVNAIAPGLVDTPLTADWADAHTLWQTRS------PMRRAAKPADIAQLATMLAANQ 231 I VN++APG VDTP+T A QTR PMRR +IA LAA + Sbjct: 178 YGITVNSVAPGPVDTPMT----QAMHSEQTRESYYRLVPMRRYGTTDEIASAVCYLAAEE 233 Query: 232 --YVTGEILTADGGL 244 Y+TG ++ DGG Sbjct: 234 SAYITGHVIPVDGGF 248 >gi|300311790|ref|YP_003775882.1| short-chain dehydrogenase/reductase family protein [Herbaspirillum seropedicae SmR1] Length = 261 Score = 111 bits (278), Expect = 4e-26, Method: Compositional matrix adjust. Identities = 85/249 (34%), Positives = 133/249 (53%), Gaps = 17/249 (6%) Query: 4 ELRVALVTGSTSGIGLALAHGFAEAGYTVVVHSRSSVETGEKLAAALPAALYI--QADLA 61 E +V +VTG+ SGIG A A F++ G V++ R + G K+ +LP + D++ Sbjct: 13 EGKVVIVTGAASGIGEATARRFSDEGARVLLADRDAAALG-KVFDSLPPERTAARETDVS 71 Query: 62 VEADRARLIAQILAHYGRLDVLVNNAGISRVIPHGDLSAASPEIWHELYEVNVVAPFRLI 121 +L+ + +G+LDVLV++AG V G+++ SPE WH + NV F Sbjct: 72 HHEQVRQLVDFAIERFGQLDVLVSDAG---VFAEGNVTEVSPEDWHRVQATNVNGVFY-- 126 Query: 122 ALAQEALKNSAKSGTPGCVINISSHAGVRPKGASIPYAATKAALNHMTKLLALSLA-PDI 180 A+EAL + K T GC++N++S +G+ Y A+K A+ ++T+ +AL + Sbjct: 127 -GAREALPHLEK--TRGCIVNVASVSGLAADWNLSAYNASKGAVCNLTRAMALDFGRKGV 183 Query: 181 RVNAIAPGLVDTPLTADWADAHTL---WQTRSPMRRAAKPADIAQLATMLAANQ--YVTG 235 R+NA+ P L T +TAD AD L + R + R A P +IA + T LA+ +V G Sbjct: 184 RINAVCPSLTHTAMTADMADDPPLLDKFAERIALGRGADPLEIAAVITFLASPDASFVNG 243 Query: 236 EILTADGGL 244 L DGGL Sbjct: 244 VNLPVDGGL 252 >gi|300310426|ref|YP_003774518.1| gluconate 5-dehydrogenase oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 264 Score = 98.2 bits (243), Expect = 5e-22, Method: Compositional matrix adjust. Identities = 82/254 (32%), Positives = 123/254 (48%), Gaps = 18/254 (7%) Query: 4 ELRVALVTGSTSGIGLALAHGFAEAGYTVVVHSRSSVETGEKLAAALPAALYIQADLAVE 63 E ++AL+TGS+ GIGLALA G A+AG +V++ R + + +AA L A + + + + Sbjct: 18 EGKLALITGSSGGIGLALARGMAQAGAAIVLNGRDAGKL-AAVAAQLRAEGHTIHESSFD 76 Query: 64 ADRAR----LIAQILAHYGRLDVLVNNAGISRVIPHGDLSAASPEIWHELYEVNVVAPFR 119 A + + +I + G + +LVNNAGI R P L WH+L N+ + F Sbjct: 77 ATSSEEVKAAVQKIESEIGPIGILVNNAGIQRRNP---LEEFKESDWHDLISTNLNSVF- 132 Query: 120 LIALAQEALKNSAKSGTPGCVINISSHAGVRPKGASIPYAATKAALNHMTKLLALSLAP- 178 A +A+ G +INI S + PY ATK A+ TK +A+ Sbjct: 133 ---FAAQAVARHMIPRGAGKIINICSVQSELGRPGIAPYTATKGAVKMFTKGMAIDWGKY 189 Query: 179 DIRVNAIAPGLVDTPLTADWAD--AHTLWQT-RSPMRRAAKPADIAQLATMLA--ANQYV 233 I+VN + PG T + + T W R+P RR D+ A LA A+++V Sbjct: 190 GIQVNGLGPGYFKTEMNMALVNDAKFTDWLVGRTPSRRWGDVEDLVGAAVFLASDASRFV 249 Query: 234 TGEILTADGGLNLT 247 G IL DGG+ T Sbjct: 250 NGHILYVDGGVTAT 263 >gi|300311568|ref|YP_003775660.1| toluenesulfonate Zinc-independent alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 252 Score = 98.2 bits (243), Expect = 6e-22, Method: Compositional matrix adjust. Identities = 81/256 (31%), Positives = 129/256 (50%), Gaps = 20/256 (7%) Query: 1 MNSELRVALVTGSTSGIGLALAHGFAEAGYTVVV---HSRSSVETGEKLAAALPAALYIQ 57 M + +VA+VTG SG G +A +A G +VV + E++ AA A++ + Sbjct: 1 MRLQDKVAIVTGGGSGFGEGIAKAYAREGAAIVVADIGEAGGLRVVEEIKAAGGRAVFAK 60 Query: 58 ADLAVEADRARLIAQILAHYGRLDVLVNNAGIS-RVIPHGDLSAASPEIWHELYEVNVVA 116 AD++ AD +L+A L H+G+LD++VNNAG + R P + + + +Y VNV + Sbjct: 61 ADVSKRADMDQLLATALEHFGKLDIVVNNAGTTHRNRP---MLEVEEDEFDRVYAVNVKS 117 Query: 117 PFRLIALAQEALKNSAKSGTPGCVINISSHAGVRPKGASIPYAATKAALNHMTKLLALSL 176 F L+ + + G INI+S AG+RP+ Y +K A+ +K +A L Sbjct: 118 IF----LSAKTFVPYFRQVGGGAFINIASTAGIRPRPGLTWYNGSKGAVITTSKSMAAEL 173 Query: 177 APD-IRVNAIAPGLVDTPLTADWADAHTLWQTRS------PMRRAAKPADIAQLATMLAA 229 PD IRVN + P + T L +++ + R PM R + P DIA L + Sbjct: 174 GPDKIRVNCVNPVISATGLLSEFMGVPDTPENRKKFVATIPMGRFSTPEDIANACLYLGS 233 Query: 230 N--QYVTGEILTADGG 243 + ++VTG + DGG Sbjct: 234 DEAEFVTGVCIEVDGG 249 >gi|300313682|ref|YP_003777774.1| short-chain alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 255 Score = 94.4 bits (233), Expect = 7e-21, Method: Compositional matrix adjust. Identities = 84/249 (33%), Positives = 121/249 (48%), Gaps = 23/249 (9%) Query: 7 VALVTGSTSGIGLALAHGFAEAGYTVVVHSRSSVETGEKLAAALPA----ALYIQADLAV 62 +ALVTG+ IG +A A++G+ V VH S E + L + AL +QA+L Sbjct: 9 IALVTGAARRIGRHIALALAQSGWDVAVHYNRSREEADTLVREIETLGRRALAVQAELGD 68 Query: 63 EADRARLIAQILAHYGRLDVLVNNAGISRVIPHGDLSAASPEIWHELYEVNVVAPFRLIA 122 E L+ Q A GR+ +VNNA + + D ++ E NV AP + Sbjct: 69 ETQVRSLLPQA-APLGRISCVVNNASL---FDYDDAASFGNERLLRHMSANVGAP---VL 121 Query: 123 LAQEALKNSAKSGTPGCVINISSHAGVRPKGASIPYAATKAALNHMTKLLALSLAPDIRV 182 LAQ AL + G VIN+ P + Y +KAAL+ T LLA +LAP +RV Sbjct: 122 LAQ-ALYEATPQGQQAVVINLLDQKLYNPNPDFLSYTLSKAALHSATTLLAQALAPKVRV 180 Query: 183 NAIAPGLVDTPLTAD-----WADAHTLWQTRSPMRRAAKPADIAQLATMLAANQYVTGEI 237 +APGL T ++ D + AH + +P+ R + P DIA +A+ + VTG Sbjct: 181 VGVAPGL--TMVSGDQTEEQFGKAHQV----TPLGRGSTPEDIAAAVCYVASARAVTGTT 234 Query: 238 LTADGGLNL 246 L DGG +L Sbjct: 235 LLVDGGQHL 243 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.317 0.130 0.371 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 751,845 Number of extensions: 29420 Number of successful extensions: 282 Number of sequences better than 1.0e-08: 49 Number of HSP's gapped: 186 Number of HSP's successfully gapped: 49 Length of query: 247 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 164 Effective length of database: 1,226,865 Effective search space: 201205860 Effective search space used: 201205860 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 129 (54.3 bits)