BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0005.0267.p (228 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310022|ref|YP_003774114.1| glutathione S-transferase [Herb... 64 9e-12 >gi|300310022|ref|YP_003774114.1| glutathione S-transferase [Herbaspirillum seropedicae SmR1] Length = 204 Score = 63.9 bits (154), Expect = 9e-12, Method: Compositional matrix adjust. Identities = 53/175 (30%), Positives = 83/175 (47%), Gaps = 20/175 (11%) Query: 10 PNPARIRIVLAQKNLDSQVTFESVDLIGAEHKQAAFLSKNPSGVLPLLELDDGTLISEST 69 P ++R+V+A+K +D + E V +Q+ NP G +P L +DDG + +S Sbjct: 11 PFVRKVRVVMAEKKIDYEFILEDVWSASTTIQQS-----NPLGKVPCLLMDDGGAMFDSR 65 Query: 70 AITEYLDNLDGNPVLTGRTPKEKAIIHMMQRRAESGLIDAVGIYFHHAT--PGLGDDLQK 127 I EYLD L L +E+A I + A+ G++DA + T P K Sbjct: 66 VIVEYLDTLTPVGKLLPPNGRERAEIKCWEALAD-GMLDAAVLVRLEKTLRP------TK 118 Query: 128 YKSPEWAHRKEWGERERAKALQGMVYFDGILRNNSYVAGEQFSMADITVFAGLAF 182 +SPEW R +W + E +L+ M DG L + G +S+AD+ V L + Sbjct: 119 QQSPEWIKR-QWSKVE--ASLKAMA--DG-LGEKPFCVGNHYSLADVAVCCALGW 167 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.135 0.394 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 706,630 Number of extensions: 27969 Number of successful extensions: 87 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 86 Number of HSP's successfully gapped: 1 Length of query: 228 Length of database: 1,619,870 Length adjustment: 82 Effective length of query: 146 Effective length of database: 1,231,600 Effective search space: 179813600 Effective search space used: 179813600 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 129 (54.3 bits)