BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0005.0253.p
         (451 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300312204|ref|YP_003776296.1| high affinity nitrate/nitrite t...   714   0.0  
gi|300312932|ref|YP_003777024.1| nitrite/nitrate transporter pro...    78   2e-15

>gi|300312204|ref|YP_003776296.1| high affinity nitrate/nitrite
           transporter transmembrane protein [Herbaspirillum
           seropedicae SmR1]
          Length = 435

 Score =  714 bits (1843), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 374/433 (86%), Positives = 402/433 (92%), Gaps = 3/433 (0%)

Query: 16  SKASKIRLFSIDTPQMRAFHLTWMAFFVCFFAWFACAPLMPVIKGQLGLTVEQIANINIA 75
           SKA++I LFS+ +PQMRAFHLTWMAFFVCFFAWFACAPLMPVIKG+  LT  QIANINIA
Sbjct: 2   SKANRIELFSLRSPQMRAFHLTWMAFFVCFFAWFACAPLMPVIKGEFHLTPGQIANINIA 61

Query: 76  AVAVTILVRLIIGPLCDRYGPRKAYTGLLLIGALPVIGVGFAQTYETFLFFRLCIGAVGA 135
           AVAVTILVRLIIGP+CDR+GPRK YTGLLL GA+PVIGV FAQ+YE+FLFFRL IGAVGA
Sbjct: 62  AVAVTILVRLIIGPMCDRFGPRKTYTGLLLAGAVPVIGVAFAQSYESFLFFRLLIGAVGA 121

Query: 136 SFVITQYHTSVMFAPNVVGTANAATAGWGNAGGGVAQGLMPLLLAAMLMLGVGDNLGWRV 195
           SFVITQYHTSVMFAPNVVGTANAATAGWGNAGGGVAQG+MPLL+AA+LMLGV    GWR 
Sbjct: 122 SFVITQYHTSVMFAPNVVGTANAATAGWGNAGGGVAQGVMPLLMAALLMLGVSQGWGWRA 181

Query: 196 ALLVPGLMMLAMAALYWHFTQDCPQGNFDELRAAGIAIESGKKGGWASFRAASANYRVWM 255
           ALLVPG++ML MAALYW FTQDCP+GN+D LRAAG+ IESGK+GGWASFRAA+ANYRVWM
Sbjct: 182 ALLVPGVLMLVMAALYWRFTQDCPEGNYDALRAAGVTIESGKRGGWASFRAAAANYRVWM 241

Query: 256 LFITYGACFGVEIFIHNIAAIYYVDHFGLSLKSAGIAAASFGLLALFARALGGIISDKVA 315
           LFITYGACFGVEIFIHNIAAIYYV+HF LSL+SAGIAAASFGLLALFARALGG ISDKVA
Sbjct: 242 LFITYGACFGVEIFIHNIAAIYYVEHFKLSLESAGIAAASFGLLALFARALGGFISDKVA 301

Query: 316 LRGGLNRRATLLFIMMLGEGLGLLWFAHAGSVTLAVTAMLCFGLFTHMACGATYALVPFI 375
           L GGLNRRATLLF+MMLGEGLGLLWFAHAGSV  AV AMLCFGLFTHMACGATYALVPFI
Sbjct: 302 LAGGLNRRATLLFVMMLGEGLGLLWFAHAGSVVAAVIAMLCFGLFTHMACGATYALVPFI 361

Query: 376 DRKALGGVAGIIGAGGNVGAVLAGFLMKGLGDTQQTLSMLGVLVTVSALCAVAVRFTTSD 435
           DRKALGGVAGIIGAGGNVGAVLAGFLMKGLG+TQQTLSMLGVLVT+SALCAVA+RFT SD
Sbjct: 362 DRKALGGVAGIIGAGGNVGAVLAGFLMKGLGNTQQTLSMLGVLVTISALCAVAIRFTASD 421

Query: 436 KNDEPTPAVAANN 448
           K+ E   AV A+N
Sbjct: 422 KSGE---AVLADN 431


>gi|300312932|ref|YP_003777024.1| nitrite/nitrate transporter
           protein [Herbaspirillum seropedicae SmR1]
          Length = 421

 Score = 77.8 bits (190), Expect = 2e-15,   Method: Compositional matrix adjust.
 Identities = 74/282 (26%), Positives = 114/282 (40%), Gaps = 36/282 (12%)

Query: 39  MAFFVCFFAWFACAPLMPVIKGQLGLTVEQIANINIAAVAVTILVRLIIGPLCDRYGPRK 98
           +AF VCF  W     +   IK  L L   +   +    V    L+R+ +G   DRYG R 
Sbjct: 16  LAFTVCFTIWMMFGVIGIPIKNALHLNETEFGLLTAMPVLTGSLIRVPLGIWTDRYGGRI 75

Query: 99  AYTGLLLIGALPVIGVGFAQTYETFLFFRLCIGAVGASFVITQYHTSVMFAPNVVGTANA 158
            +  L+L   +P+  + +A  +  FL   L +G  G SF +   + +  F     G A  
Sbjct: 76  VFFLLMLSTVIPIWLISYAHAFWHFLVLGLFVGLAGGSFSVGTPYVARWFKKERQGLA-M 134

Query: 159 ATAGWGNAGGGVAQGLMPLLLAAMLMLGVGDNLGWRVALLVPGLMMLAMAALYWHFTQDC 218
              G GN+G  V + + P+L+A+          GW +   V   +MLA A ++W  +   
Sbjct: 135 GVFGAGNSGAAVNKFIAPVLIASA---------GWTLVPQVYAGLMLAAALIFWLGSATD 185

Query: 219 PQGN-------FDELRAAGIAIESGKKGGWASFRAASANYRVWMLFITYGACFGVEIFIH 271
           P           D++R                      + RVW     Y   FG  + + 
Sbjct: 186 PSHQVSSDTRFMDQVRML-------------------RDPRVWRYCQYYSVVFGGYVGLS 226

Query: 272 NIAAIYYVDHFGLSLKSAGIAAASFGLLALFARALGGIISDK 313
                YYV  +G  ++ A + AA F L     RALGG +SD+
Sbjct: 227 LWMVKYYVTEYGFDIRHAALLAACFSLPGGVLRALGGWLSDR 268


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.328    0.140    0.436 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 1,067,704
Number of extensions: 35464
Number of successful extensions: 231
Number of sequences better than 1.0e-08: 4
Number of HSP's gapped: 238
Number of HSP's successfully gapped: 4
Length of query: 451
Length of database: 1,619,870
Length adjustment: 88
Effective length of query: 363
Effective length of database: 1,203,190
Effective search space: 436757970
Effective search space used: 436757970
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 132 (55.5 bits)