BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0005.0249.p (434 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312207|ref|YP_003776299.1| FAD-dependent pyridine nucleoti... 548 e-157 gi|300312205|ref|YP_003776297.1| nitrite reductase large subunit... 151 1e-37 gi|300312608|ref|YP_003776700.1| glutathione reductase [Herbaspi... 60 3e-10 >gi|300312207|ref|YP_003776299.1| FAD-dependent pyridine nucleotide-disulfide oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 413 Score = 548 bits (1412), Expect = e-157, Method: Compositional matrix adjust. Identities = 269/312 (86%), Positives = 287/312 (91%), Gaps = 1/312 (0%) Query: 10 SAKKPSLVVIGNGMAGMRTVEELLKLAPDLYDITVFGAEPHGNYNRILLSPVLAGDKSID 69 S +PSLVV+GNGMAGMRTVEELLKLAPDLYDITVFGAEPHGNYNRI+LSPVLAGDKSID Sbjct: 8 STARPSLVVVGNGMAGMRTVEELLKLAPDLYDITVFGAEPHGNYNRIMLSPVLAGDKSID 67 Query: 70 DIMLNTRDWYVQNNITLHAGDPVEKIDRRRRTVRSQAGVEIKYDRLLLATGSKPFIIPVP 129 DIMLN R WY N ITLHAGDPV IDRR+RTV +++G+ ++YDRLLLATGS PFIIPVP Sbjct: 68 DIMLNPRAWYDDNGITLHAGDPVVHIDRRQRTVHAKSGLTVQYDRLLLATGSTPFIIPVP 127 Query: 130 GHELPGVIAFRDIQDVHIMLDVARNHRHAVVIGGGLLGLEAANGLLRQGMDVTVVHVTDS 189 GH+LPGVIAFRDIQDV ML AR HRHAVVIGGGLLGLEAANGL+RQGMDVTVVHVTD+ Sbjct: 128 GHQLPGVIAFRDIQDVDAMLQAARTHRHAVVIGGGLLGLEAANGLMRQGMDVTVVHVTDA 187 Query: 190 LMNQQLDKPAAALLKKALEMKGLRFLLNAQTEAIVGPDRVTAVRFKDGSEIPADLVVMTA 249 LMNQQLDKPAAALLKKALE KGLRFLLNA TE IVGPDRVTAVRFKDGS IPADLVVMTA Sbjct: 188 LMNQQLDKPAAALLKKALEDKGLRFLLNAHTEEIVGPDRVTAVRFKDGSSIPADLVVMTA 247 Query: 250 GVRPNIGLAKEAGLHCDRAIVVDDTLQTYDPRIYAVGECVQHRQATFGLVAPIWDQARVC 309 GVRPNI LAK AGLHC+RAI+VDDTLQTYDPR+YAVGECVQHR+ATFGLVAPIWDQARVC Sbjct: 248 GVRPNIALAKAAGLHCERAIIVDDTLQTYDPRVYAVGECVQHRKATFGLVAPIWDQARVC 307 Query: 310 GAHLAGGSGWRR 321 GAHLA G+G RR Sbjct: 308 GAHLA-GAGHRR 318 >gi|300312205|ref|YP_003776297.1| nitrite reductase large subunit [Herbaspirillum seropedicae SmR1] Length = 857 Score = 151 bits (382), Expect = 1e-37, Method: Compositional matrix adjust. Identities = 112/310 (36%), Positives = 171/310 (55%), Gaps = 15/310 (4%) Query: 16 LVVIGNGMAGMRTVEELLKLAPDLYDITVFGAEPHGNYNRILLSPVLAGDKSIDDIMLNT 75 ++VIG+GM G + +E L + +T+ EP Y+R+ LS AG K+ +D+ L Sbjct: 3 IIVIGHGMVGHKFLECLAESGAGDLQVTILCEEPRPAYDRVHLSEFFAG-KTAEDLSLVA 61 Query: 76 RDWY------VQNNITLHAGDPVEKID-RRRRTVRSQAGVE--IKYDRLLLATGSKPFII 126 ++ VQ ++ L+A ++ID R++ + + AGVE + YD+L+LATGS PF+ Sbjct: 62 PGFFDRGRSAVQFDLKLNA--KAQQIDLERKQVLVNVAGVEETMSYDKLVLATGSYPFVP 119 Query: 127 PVPGHELPGVIAFRDIQDVHIMLDVARNHRHAVVIGGGLLGLEAANGLLRQGMDVTVVHV 186 V G++ +R I+D+ ML+ + VVIGGGLLGLE A L ++ VV Sbjct: 120 TVAGNQRKDCFVYRTIEDLEAMLECGSRSKIGVVIGGGLLGLECAKALRDLKLETHVVEF 179 Query: 187 TDSLMNQQLDKPAAALLKKALEMKGLRFLLNAQTEAIV-GPDRVTAVRFKDGSEIPADLV 245 LM Q+D+ +L++ +E G+ T IV G + F+DGS + D++ Sbjct: 180 APRLMAVQVDESGGRVLRQKIEELGVTAHTQKNTVEIVDGKHHTHRMNFEDGSHLETDMI 239 Query: 246 VMTAGVRPNIGLAKEAGLHC-DR-AIVVDDTLQTYDPRIYAVGECVQHRQATFGLVAPIW 303 V +AG+RP LA++AGL DR I +D++ T DP +YA+GEC FGLVAP + Sbjct: 240 VFSAGIRPRDELARQAGLKVGDRGGIAIDNSCLTSDPAVYAIGECALWNGKIFGLVAPGY 299 Query: 304 DQARVCGAHL 313 D AR HL Sbjct: 300 DMARTAAKHL 309 >gi|300312608|ref|YP_003776700.1| glutathione reductase [Herbaspirillum seropedicae SmR1] Length = 458 Score = 60.5 bits (145), Expect = 3e-10, Method: Compositional matrix adjust. Identities = 70/240 (29%), Positives = 108/240 (45%), Gaps = 41/240 (17%) Query: 68 IDDIMLNTRDWYVQNNITLHAG-DPVE-----KIDRRRRTVRSQAGVEIKYDRLLLATGS 121 ++DI L D ++LH G VE +D +R T R +L+ATG Sbjct: 96 LNDIYLRLLD---NAKVSLHRGFAKVEDAHTVNVDGQRFTAR----------HILVATGG 142 Query: 122 KPFIIPVPGHELPGVIAFRDIQDVHIMLDVARNHRHAVVIGGGLLGLEAANGLLRQGMDV 181 +P +PG EL I D + + + AVV+GGG + +E A+ L G +V Sbjct: 143 RPDKPAIPGAELG--ITSDDFFHLKAL------PQRAVVLGGGYIAVELASILNGLGCEV 194 Query: 182 TVVHVTDSLMNQQLDKPAAALLKKALEMKGLRFLLNAQTEAI-------VGPDRVTAVRF 234 T+V+ + L+ + +D L + KG+R + NA EAI V VR Sbjct: 195 TLVYRRERLL-RNMDADLGIHLADEMAKKGIRIVFNASIEAIEAEQAGEASEASVKTVRL 253 Query: 235 KDGSEIPADLVVMTAGVRPN---IGLAKEAG--LHCDRAIVVDDTLQTYDPRIYAVGECV 289 +G + A V+ G N +GL +EAG L + AI V+ ++ P I AVG+ + Sbjct: 254 TNGEALSAQCVLFATGRTANTAGLGL-QEAGVKLKANGAIEVNQDFESSVPSILAVGDVI 312 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.138 0.414 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,214,865 Number of extensions: 46440 Number of successful extensions: 153 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 148 Number of HSP's successfully gapped: 3 Length of query: 434 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 346 Effective length of database: 1,203,190 Effective search space: 416303740 Effective search space used: 416303740 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 132 (55.5 bits)