BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0005.0242.p (64 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310476|ref|YP_003774568.1| major facilitator superfamily t... 86 7e-19 gi|300310490|ref|YP_003774582.1| major facilitator superfamily p... 83 4e-18 >gi|300310476|ref|YP_003774568.1| major facilitator superfamily transporter protein [Herbaspirillum seropedicae SmR1] Length = 449 Score = 85.5 bits (210), Expect = 7e-19, Method: Compositional matrix adjust. Identities = 44/53 (83%), Positives = 47/53 (88%) Query: 1 VIALINSLGNLGGFVAPTTFGFLEQRTGSIQGGLYALATTSVVAAVVVFFART 53 VIALINS+GNLGGFVAPT FGFLEQRTGSIQGGL LA TS+VAA VVF AR+ Sbjct: 388 VIALINSIGNLGGFVAPTAFGFLEQRTGSIQGGLMGLAITSLVAAGVVFLARS 440 >gi|300310490|ref|YP_003774582.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 447 Score = 83.2 bits (204), Expect = 4e-18, Method: Compositional matrix adjust. Identities = 40/53 (75%), Positives = 48/53 (90%) Query: 1 VIALINSLGNLGGFVAPTTFGFLEQRTGSIQGGLYALATTSVVAAVVVFFART 53 V+ALINSLGNLGGF AP TFG+LEQ+TGSIQGGL+AL+ S++AAV+VF ART Sbjct: 376 VLALINSLGNLGGFFAPATFGYLEQKTGSIQGGLFALSGASLLAAVLVFLART 428 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.135 0.372 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 145,256 Number of extensions: 3177 Number of successful extensions: 34 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 34 Number of HSP's successfully gapped: 3 Length of query: 64 Length of database: 1,619,870 Length adjustment: 36 Effective length of query: 28 Effective length of database: 1,449,410 Effective search space: 40583480 Effective search space used: 40583480 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 123 (52.0 bits)