BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0005.0241.p (254 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310476|ref|YP_003774568.1| major facilitator superfamily t... 384 e-108 gi|300310490|ref|YP_003774582.1| major facilitator superfamily p... 374 e-105 gi|300311567|ref|YP_003775659.1| major facilitator superfamily p... 170 1e-43 gi|300313516|ref|YP_003777608.1| major facilitator superfamily p... 163 2e-41 gi|300311164|ref|YP_003775256.1| major facilitator superfamily p... 159 2e-40 >gi|300310476|ref|YP_003774568.1| major facilitator superfamily transporter protein [Herbaspirillum seropedicae SmR1] Length = 449 Score = 384 bits (986), Expect = e-108, Method: Compositional matrix adjust. Identities = 204/251 (81%), Positives = 221/251 (88%) Query: 1 VLRFLLGVAEAGFFPGVIYYFTQWLPNGERGKAMAIFLSGSALASILSGPISGALLQIEG 60 VLRFLLG AEAGFFPGVIYYFTQWLP ERG+AMAIFLSGSALASILSGPISG LLQI+G Sbjct: 129 VLRFLLGAAEAGFFPGVIYYFTQWLPASERGRAMAIFLSGSALASILSGPISGGLLQIDG 188 Query: 61 GGLHXWQWMFIIEGLASVVLCGFVWFWLDSRPADAKWLTQSERDLIGNTIAAEQAEREKI 120 GGL WQWMFIIEG+ASV+LCGFVWFWLDS PADAKWLT ER I I EQAER K Sbjct: 189 GGLQGWQWMFIIEGMASVLLCGFVWFWLDSMPADAKWLTAQERSAITRAIVDEQAERMKH 248 Query: 121 KPAHISTWALLRDSQIVIFCFIYFSISLTIYGATFWLPSIIRKMGAYSDFQVGLFNSIPW 180 +PA +S LLRD QI+IFCFIYFSISLTIYGATFWLPSIIRKMG++SDFQVGLFNSIPW Sbjct: 249 QPAQVSPRQLLRDPQILIFCFIYFSISLTIYGATFWLPSIIRKMGSFSDFQVGLFNSIPW 308 Query: 181 IISIIAMYLFASLAARFKFQQAWAAAALIIAAIGMYVSTFGGPVFAFVSICFAAVGFKAA 240 +ISI+AMY FA+LAARFK QQAWAA AL+IAA GM+ STFG V+AFVSICFAAVGFKAA Sbjct: 309 LISIVAMYAFAALAARFKHQQAWAATALVIAAAGMFASTFGNSVYAFVSICFAAVGFKAA 368 Query: 241 SSLFWPIPQSY 251 SSLFWP+PQ+Y Sbjct: 369 SSLFWPLPQAY 379 >gi|300310490|ref|YP_003774582.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 447 Score = 374 bits (961), Expect = e-105, Method: Compositional matrix adjust. Identities = 177/250 (70%), Positives = 209/250 (83%) Query: 2 LRFLLGVAEAGFFPGVIYYFTQWLPNGERGKAMAIFLSGSALASILSGPISGALLQIEGG 61 +RFLLGVAEAGFFPGV+YYFTQWLPN ERG+AMA+FLSGSA+ASILSGPI+GALL IEGG Sbjct: 118 MRFLLGVAEAGFFPGVVYYFTQWLPNHERGRAMAVFLSGSAIASILSGPITGALLLIEGG 177 Query: 62 GLHXWQWMFIIEGLASVVLCGFVWFWLDSRPADAKWLTQSERDLIGNTIAAEQAEREKIK 121 GLH WQWMFIIEG AS+V C VW LDS P +AKWL+Q +R+ + IAAEQ +R + Sbjct: 178 GLHGWQWMFIIEGGASMVFCVVVWLVLDSTPREAKWLSQEQREALAAVIAAEQEQRAANR 237 Query: 122 PAHISTWALLRDSQIVIFCFIYFSISLTIYGATFWLPSIIRKMGAYSDFQVGLFNSIPWI 181 PAH+S +LLRD QIV+FCFIYF+I LTIYGATFWLP+II+KMG ++DFQVGLFNSIPWI Sbjct: 238 PAHVSALSLLRDPQIVLFCFIYFAIQLTIYGATFWLPTIIKKMGHFNDFQVGLFNSIPWI 297 Query: 182 ISIIAMYLFASLAARFKFQQAWAAAALIIAAIGMYVSTFGGPVFAFVSICFAAVGFKAAS 241 ++I AMYLFA LAAR+K Q W A L+I+A GM +S GPV FV++CFA +GFKAAS Sbjct: 298 VAIAAMYLFAVLAARYKKPQFWVATTLVISAAGMAISAVAGPVIGFVAVCFAGIGFKAAS 357 Query: 242 SLFWPIPQSY 251 SLFWPIPQ+Y Sbjct: 358 SLFWPIPQAY 367 >gi|300311567|ref|YP_003775659.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 435 Score = 170 bits (431), Expect = 1e-43, Method: Compositional matrix adjust. Identities = 94/255 (36%), Positives = 144/255 (56%), Gaps = 9/255 (3%) Query: 1 VLRFLLGVAEAGFFPGVIYYFTQWLPNGERGKAMAIFLSGSALASILSGPISGALLQIEG 60 ++RFLLGVAEAGFFPGVI Y T W P RGK A+F++G AL+ ++ GP+SG ++ Sbjct: 120 IMRFLLGVAEAGFFPGVILYLTYWYPAARRGKITALFMTGIALSGVIGGPLSGWIMHAMP 179 Query: 61 G--GLHXWQWMFIIEGLASVVLCGFVWFWLDSRPADAKWLTQSERDLIGNTIAAEQAERE 118 G G WQWMFI+E + S++L V ++ R DA WL++ E+ L+ I+ E++++E Sbjct: 180 GVWGHTGWQWMFILEAIPSLILGVMVILYMKDRIRDADWLSEEEKRLLEANISHEESQKE 239 Query: 119 KIKPAHISTWALLRDSQIVIFCFIYFSISLTIYGATFWLPSIIRKMGAYSDFQVGLFNSI 178 H+S A+ + ++ + IYF + +YG +FWLP+II+ G VGL +I Sbjct: 240 -----HLSLGAMFANGRVWLGALIYFCFVMGLYGVSFWLPTIIKATGVSDPLNVGLLTAI 294 Query: 179 PWIISIIAMYLFASLAARFKFQQAWAAAALIIAAIGMYVSTFGG--PVFAFVSICFAAVG 236 P+ + AM L A + ++ A I IG+ +ST G A S+ A+VG Sbjct: 295 PYAFAAAAMVLIGRSADARRERRWHVAIPAFIGCIGLLLSTQYGHNTTLAMASLTLASVG 354 Query: 237 FKAASSLFWPIPQSY 251 LFW +P ++ Sbjct: 355 ILTTLPLFWSLPTAF 369 >gi|300313516|ref|YP_003777608.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 437 Score = 163 bits (412), Expect = 2e-41, Method: Compositional matrix adjust. Identities = 91/259 (35%), Positives = 147/259 (56%), Gaps = 16/259 (6%) Query: 1 VLRFLLGVAEAGFFPGVIYYFTQWLPNGERGKAMAIFLSGSALASILSGPISGALLQI-- 58 +LRFLLG+AEAGF+PG+I Y T W P+ R K +A+F+SG +A IL P+SG ++ Sbjct: 121 ILRFLLGLAEAGFYPGIILYLTYWFPSHRRAKVIAVFMSGIPVAGILGNPLSGWIMDAFH 180 Query: 59 EGGGLHXWQWMFIIEGLASVVLCGFVWFWLDSRPADAKWLTQSERDLIGNTIAAEQAERE 118 + GGL WQWMF+IE + +V++ +LD+ AKWL E+ ++ A+ Sbjct: 181 QNGGLEGWQWMFLIEAIPAVLIGVATVLYLDNDVKSAKWLNDEEK----ASLQADIDGDA 236 Query: 119 KIKPAHISTWALLRDSQIVIFCFIYFSISLTIYGATFWLPSIIRKMGAYSDFQVGLFNSI 178 K K + A+++D+++ + C IYFS + YG T W+P++++ G + ++GL ++I Sbjct: 237 KGKESKHGFGAIVKDARVWLMCLIYFSFVMGQYGLTLWMPTLVKATGVKGNLEIGLLSAI 296 Query: 179 PWIISIIAMYLFASLAARFKFQQAWAAAALIIAAIGMYVSTFGGPVFA------FVSICF 232 P+ +IIAM L A R + ++ W L+I A+ V G +FA S+ Sbjct: 297 PFGCAIIAMNLIGRSADRMR-ERRW---HLVIPALMGGVGFVGAALFADNTAVSIASLSL 352 Query: 233 AAVGFKAASSLFWPIPQSY 251 AA G + LFW +P ++ Sbjct: 353 AAAGVLTCAPLFWSLPTAF 371 >gi|300311164|ref|YP_003775256.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 437 Score = 159 bits (402), Expect = 2e-40, Method: Compositional matrix adjust. Identities = 95/253 (37%), Positives = 146/253 (57%), Gaps = 10/253 (3%) Query: 1 VLRFLLGVAEAGFFPGVIYYFTQWLPNGERGKAMAIFLSGSALASILSGPISGALLQ-IE 59 +LRFLLG+ EAGFFPGV+ Y T W P R + +F++ A+A + GPI+G ++ +E Sbjct: 124 ILRFLLGIFEAGFFPGVVLYLTYWFPAQRRARVNGLFMTSFAIAGAVGGPIAGLIMSGME 183 Query: 60 G-GGLHXWQWMFIIEGLASVVLCGF-VWFWLDSRPADAKWLTQSERDLIGNTIAAEQAER 117 G G L WQW+FI+EG+ S +L GF V WL RPA+AKWL+++E++ + +AAE + Sbjct: 184 GVGHLANWQWLFILEGIPS-ILAGFAVLAWLPERPANAKWLSEAEKNAVTQVLAAENSAP 242 Query: 118 EKIKPAHISTWALLRDSQIVIFCFIYFSISLTIYGATFWLPSIIRKMGAYSDFQVGLFNS 177 K H S + ++ I +YF + FW PSII+++G + Q+GL ++ Sbjct: 243 GK----HFSFKDACTNYRVWICAAVYFCVVSGNATIAFWAPSIIKEIGVKGNLQIGLISA 298 Query: 178 IPWIISIIAMYLFASLAARFKFQQAWAAAALIIAAIGMYVS--TFGGPVFAFVSICFAAV 235 IP+I +AM + + ++ +A A +IA IG+ + T G A S+ AA+ Sbjct: 299 IPFIAGTLAMIWNGFHSDKTGERRLHSAIATLIACIGLVATGMTLGNATLALCSLTLAAI 358 Query: 236 GFKAASSLFWPIP 248 G AA +FW IP Sbjct: 359 GILAAFPVFWSIP 371 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.329 0.140 0.458 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 737,919 Number of extensions: 26422 Number of successful extensions: 177 Number of sequences better than 1.0e-08: 14 Number of HSP's gapped: 163 Number of HSP's successfully gapped: 14 Length of query: 254 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 171 Effective length of database: 1,226,865 Effective search space: 209793915 Effective search space used: 209793915 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 129 (54.3 bits)