BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0005.0222.p (198 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311438|ref|YP_003775530.1| transmembrane protein [Herbaspi... 157 6e-40 >gi|300311438|ref|YP_003775530.1| transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 200 Score = 157 bits (397), Expect = 6e-40, Method: Compositional matrix adjust. Identities = 91/153 (59%), Positives = 119/153 (77%) Query: 9 LLAPFVYTAALLLLLEEWLWDASKRLSARLPLIPFIVRLEAWVAGVPPYVALVIFVLPSL 68 LLAP +YTAALLL+LEEWLWDAS+ + AR+P P +VRL+ WV + PY AL+IF+ P+L Sbjct: 18 LLAPLIYTAALLLMLEEWLWDASQAVLARIPAWPLLVRLQRWVERLSPYAALLIFLAPTL 77 Query: 69 LLFPVKILALMSITHGHPTLGLIIIVSAKVLGTALVARVYTLTQKALLSLPWFARYRDII 128 LL PVKILAL+SITHGHPTLGL I++ AKVLGTALVAR+Y LT+++LLSL WF RY D + Sbjct: 78 LLLPVKILALLSITHGHPTLGLCIVLIAKVLGTALVARIYALTRRSLLSLAWFQRYHDKL 137 Query: 129 LRFKDRMIAQLRATHAWQQVEAGLAAFKEARRR 161 + K R++AQL A+ W+Q++ + +E R Sbjct: 138 MEIKARLLAQLHASAGWRQLQRLRQSLREGMLR 170 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.330 0.140 0.445 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 516,111 Number of extensions: 18128 Number of successful extensions: 92 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 92 Number of HSP's successfully gapped: 1 Length of query: 198 Length of database: 1,619,870 Length adjustment: 80 Effective length of query: 118 Effective length of database: 1,241,070 Effective search space: 146446260 Effective search space used: 146446260 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.9 bits) S2: 128 (53.9 bits)