BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0005.0218.p (578 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311435|ref|YP_003775527.1| chloride channel protein [Herba... 857 0.0 gi|300310913|ref|YP_003775005.1| chloride channel EriC protein [... 127 2e-30 gi|300310046|ref|YP_003774138.1| chloride-channel protein [Herba... 75 1e-14 gi|300311782|ref|YP_003775874.1| chloride channel protein [Herba... 69 8e-13 >gi|300311435|ref|YP_003775527.1| chloride channel protein [Herbaspirillum seropedicae SmR1] Length = 555 Score = 857 bits (2215), Expect = 0.0, Method: Compositional matrix adjust. Identities = 439/555 (79%), Positives = 498/555 (89%) Query: 24 MLLWAAVVGFVGALATIGFRECIDAVQYLLTGHSGSFVEIAKGLSWQMRVLLPTCGGVVA 83 MLLW A+ GFVGALATI FRECI +Q +TGH GSFV+IAKGL W RVLLPTCGGVVA Sbjct: 1 MLLWGALAGFVGALATIAFRECIALLQIWMTGHDGSFVDIAKGLPWYTRVLLPTCGGVVA 60 Query: 84 GLILIWAKRMPAGATSDYMEAVAIGDGRIPIRHTLLRSLSSLATVGSGGSIGREGPMVQL 143 GL L+WAKR PAG DYMEAVAIGDG IP+R+TLLRS+SSLA++ SGGSIGREGPMVQL Sbjct: 61 GLFLVWAKRTPAGNGGDYMEAVAIGDGAIPVRNTLLRSISSLASIASGGSIGREGPMVQL 120 Query: 144 AALCASLVGRFAHFPSSRMRLLVACGAAAGITSAYNAPIAGAFFVTEIVLGALVMESFGP 203 +ALCASLVG+F+HFPSSR+RLLVACGAAAGITSAYNAPIAGAFF+TEIVLG+LVMESFGP Sbjct: 121 SALCASLVGKFSHFPSSRLRLLVACGAAAGITSAYNAPIAGAFFITEIVLGSLVMESFGP 180 Query: 204 VVVASVVANITMRELPGYKPAYEMPYFPEVAGVEIILFVVLGVIAGLLAPQFLRLLELSK 263 VVVASVVANITMRELPGY+ AYEMPYFPE+ G E++LF+VLGV+AG+LAPQFLR+LEL K Sbjct: 181 VVVASVVANITMRELPGYRAAYEMPYFPEIGGWEVMLFLVLGVLAGVLAPQFLRVLELGK 240 Query: 264 TAFRKTRLPLPLRLGLGGLLVGLLSVRVPEVWGNGYSVVNSLLHTDWLWSTVLIVMIYKI 323 F K RLPLP+RLG+GGLLVGL+SV+VPEVWGNGYS+VNSLLHT W+W VL+V++ K+ Sbjct: 241 HGFGKLRLPLPVRLGVGGLLVGLISVQVPEVWGNGYSLVNSLLHTSWVWQAVLMVLVVKV 300 Query: 324 VATALTTGSGAVGGIFTPTLFVGAAIGFLFGDMAHALWPHAISQPFAYAMVGMGAFLAAA 383 +AT++T GSGAVGGIFTPTLFVGAA+G+LFGD A AL P ISQPFAYAMVGMGAFLAAA Sbjct: 301 LATSITVGSGAVGGIFTPTLFVGAAVGYLFGDAAQALLPVHISQPFAYAMVGMGAFLAAA 360 Query: 384 TNAPLMAILMIFEMTLSYQVVLPLMLSCVVAYVIARSLDGNSMYEITLKRHRDAAERIRL 443 + APLMAILMIFEMTLSYQVVLPLMLSCVVAYV+ARS+DG SMYEITLKRHRD ER+RL Sbjct: 361 SYAPLMAILMIFEMTLSYQVVLPLMLSCVVAYVVARSVDGRSMYEITLKRHRDNEERLRL 420 Query: 444 RGTHMSELIKPAETVLQKDAPFSEVTRIFLQYPVKYIYITDQENRYLGVVALQDVTSLLL 503 RGTHMSELI+PAETVL ++A E++ +FL+YPVKY+YI D RYLGVVALQD+TS LL Sbjct: 421 RGTHMSELIRPAETVLHEEASLEEISALFLKYPVKYVYIVDGRQRYLGVVALQDITSWLL 480 Query: 504 DKDDIATKCAGDFMRTHFLHEVTPDMSLGEALQLFLDHQGERLPVISSAEDPRLLGVVFK 563 DK + A + A DF+R HFLHEVTPDMSLGEALQLFLDHQGERLPVI+SA+DP+LLGVVFK Sbjct: 481 DKKEAAGRVARDFLRPHFLHEVTPDMSLGEALQLFLDHQGERLPVIASADDPQLLGVVFK 540 Query: 564 TSLLDAYFRLDRSGE 578 TSLLDAYFRLDR+G Sbjct: 541 TSLLDAYFRLDRAGN 555 >gi|300310913|ref|YP_003775005.1| chloride channel EriC protein [Herbaspirillum seropedicae SmR1] Length = 572 Score = 127 bits (319), Expect = 2e-30, Method: Compositional matrix adjust. Identities = 154/523 (29%), Positives = 236/523 (45%), Gaps = 49/523 (9%) Query: 67 LSWQMRVLLPTCGGVVAGLILIW-AKRMPAGATSDYMEAVAIGDGRIPIRHTLLRSLSSL 125 L W + LLP GG++ GL+ + + ++ + +EA+ G R+ + LL+ LSS Sbjct: 62 LGW-IAALLPIAGGLIVGLMARYGSDKIRGHGIPEAIEAILFGKSRMSPKVALLKPLSSG 120 Query: 126 ATVGSGGSIGREGPMVQLAALCASLVGRFAHFPSSRMRLLVACGAAAGITSAYNAPIAGA 185 +GSGG G EGP++ SL+ + H ++ + L+ GA AG+T+ + P+A Sbjct: 121 IVIGSGGPFGAEGPIIMTGGSIGSLLAQLLHLTAAERKTLLVAGACAGMTAIFGTPLAAV 180 Query: 186 FFVTEIVLGALVMESFGPVVVASVVANITMRELPGYKPAYEMPYFP-EVAG---VEIILF 241 E++L L S PV +A VA L P FP +VA V ++ Sbjct: 181 LLAVELLLFELKPRSLLPVALACAVAGFVRPWL-----VEAGPLFPLDVAAPTPVALLSC 235 Query: 242 VVLGVIAGLLAPQFLRLLELSKTAFRKTRLPLPLRLGLGGLLVGLLSVRVPEVWGNGYSV 301 +V G+ +G+LA L + F + RL LGG+ VGL + P G GY V Sbjct: 236 LVAGLASGVLAAALTLALYRIEDGFHRLRLHWMWWPALGGIAVGLGGLLQPRALGVGYDV 295 Query: 302 VNSLLHTDWLWSTVLIVMIYKIVATALTTGSGAVGGIFTPTLFVGAAIG-----FLFGDM 356 + LLH L S L +++ K V L GSG GG+ P L +GA +G +L G Sbjct: 296 IGDLLHQHMLLSAALALLLVKAVIWLLALGSGTSGGVLAPLLMLGAGLGLVLSPWLPGG- 354 Query: 357 AHALWPHAISQPFAYAMVGMGAFLAAATNAPLMAILMIFEMTLSYQVVLPLMLSCVVAY- 415 + LWP +V M L + APL AI+ F +T + Q +LPL+ + VAY Sbjct: 355 SDGLWP----------LVCMAGVLGSVLGAPLTAIVFAFGLTHASQALLPLIATTAVAYG 404 Query: 416 ----VIARSLDGNSMYEITLKRHR----DAAERIRLRGTHMSELIKPAETVLQKDAPFSE 467 ++ RS+ + L HR D ER+ H+ EL+ A L P +E Sbjct: 405 VNVLLMKRSIMTEKIARRGLHVHREYGVDPLERM-----HVEELMTAAPQTLAGALPAAE 459 Query: 468 -VTRIFLQYPVKYIYITDQENRYLGVVALQDVTSLLLDKDDIATKCAGDFMRTHFLHEVT 526 + Q V Y +G++AL+ L+ D + C D + H + Sbjct: 460 ALAWCRAQEHVHRYYPVTDGVHLVGMLALRA-----LEHADASQPC--DTLAQGHEHYLL 512 Query: 527 PDMSLGEALQLFLDHQGERLPVISSAEDPRLLGVVFKTSLLDA 569 P MS A + RLPV++ E +L+GV+ + L A Sbjct: 513 PHMSARSAAGMMARLGVARLPVVAGPERRKLVGVLSLSDLARA 555 >gi|300310046|ref|YP_003774138.1| chloride-channel protein [Herbaspirillum seropedicae SmR1] Length = 441 Score = 75.5 bits (184), Expect = 1e-14, Method: Compositional matrix adjust. Identities = 121/448 (27%), Positives = 191/448 (42%), Gaps = 58/448 (12%) Query: 17 RMSESHAMLLW---------AAVVGFVGALATIGFRECIDAVQYLLTGHSGSFVEIAKGL 67 ++S+SH LW A +VG V L + + I+ G F I Sbjct: 19 QLSKSHLRRLWVEYGILWSGAVIVGLVAVL----YAQAIEI-------GFGVFRSIQHRS 67 Query: 68 SWQMRVLLPTCGGVVAGLILIWAKRMPAGATSDYMEAVAIGDGRIPIRHTLLRSLS---- 123 W +L P V A + + + P S +A+A G R + L SL Sbjct: 68 PWLPLLLTPA---VCAACVWLTRRYFPGSEGSGIPQAIATLHGDTAERGSTLLSLRVLLG 124 Query: 124 ----SLATVGSGGSIGREGPMVQLAALCASLVGRFAHFPSSRM-RLLVACGAAAGITSAY 178 S+ + G +IGREGP VQ+ A + R M R L+ GAAAG+ +A+ Sbjct: 125 KIVLSVVAIAGGMTIGREGPTVQIGAAIMYNLRRLYPAGYEHMERRLILAGAAAGLAAAF 184 Query: 179 NAPIAGAFFVTEIVLGALVMESFGPVVVASVVANITMRELPGYKPAYEMPYFP-EVAG-- 235 N P+AG F E + + + G V+ + A + + G + FP E +G Sbjct: 185 NTPLAGIVFAIEELSRSFEQRASGVVITTIIFAGLVALSINGNYTYFGSIAFPGETSGRL 244 Query: 236 --VEIILFVVLGVIAGLLAPQFLRLLELSKTAFRKTRLPLPLRL-GLGGLLVGLLSVRV- 291 I+ V++GV GL L A R P+ + G +V ++ V Sbjct: 245 ILAVIVTAVLMGVAGGLFCWLILNPTRWIPPALVTLRTERPVAFAAVCGFIVAVIGVAAG 304 Query: 292 PEVWGNGYSVVNSLLHTDWLWSTVLIVMIYKIVATALTTGS---GAVGGIFTPTLFVGAA 348 +G+GY + LL+ S + Y + A GS G GGIF P+L +GA Sbjct: 305 GATFGSGYEQAHGLLNDGQGLS-----VFYPFLKFASMIGSYLPGLPGGIFAPSLSIGAG 359 Query: 349 IGFLFGDMAHALWPHAISQPF--AYAMVGMGAFLAAATNAPLMAILMIFEMTLSYQVVLP 406 FG++ H L+ S P A AMVG +LAA T +P+ A +++ EM + +V+ Sbjct: 360 ----FGNLLHLLF-GGTSLPMLVALAMVG---YLAAVTQSPITAFVIVMEMVDGHALVIS 411 Query: 407 LMLSCVVAYVIARSLDGNSMYEITLKRH 434 LM + ++A +++ +YE +R+ Sbjct: 412 LMATALIASAVSKVF-APPLYETLAERY 438 >gi|300311782|ref|YP_003775874.1| chloride channel protein [Herbaspirillum seropedicae SmR1] Length = 442 Score = 69.3 bits (168), Expect = 8e-13, Method: Compositional matrix adjust. Identities = 103/358 (28%), Positives = 152/358 (42%), Gaps = 36/358 (10%) Query: 74 LLPTCGGVVAGLILIWAKRMPAGATSDYMEAVAIGDGR--IPIRHTLLRSLSSLATVGSG 131 LLP G V L L K + AG E I D R IP+R L L ++A+ G Sbjct: 57 LLPLGGFAVGWLYLHTGKPVEAGNNLLIDE---IHDPRKVIPLRMAPLVLLGTVASHLFG 113 Query: 132 GSIGREGPMVQLAALCASLVGRFAHFPSSRMRLLVACGAAAGITSAYNAPIAGAFFVTEI 191 S+GREG VQ+ A + R+L+ G +AG +S + P+AGA F E+ Sbjct: 114 ASVGREGTAVQMGGALADQLTHLLRLRPEDRRILLMAGISAGFSSVFGTPMAGAIFGLEV 173 Query: 192 V-LGALVMESFGPVVVASVVANITMRELPGYKPAYEMPYFPEVAGVEIILFVVLGVIAGL 250 + +G + E+ P VA++ A+ + Y M +A + + GV+ GL Sbjct: 174 LAIGRMRYEAIFPCFVAAIAADQVGLAWGVHHTHYAMNASAPMALWSVAAVIAAGVVFGL 233 Query: 251 LAPQFLRLLELSKTAFRKTRLPL-PLRLGLGGLLVGLLSVRVPEVWGNGYS--------- 300 F + +AF K + PLR +GG +V L VW G Sbjct: 234 AGMIFANSTH-TLSAFMKRHVSYAPLRPFIGGFIVAL------AVWAVGTHRYIGLGIPV 286 Query: 301 VVNSLLHTDWLWSTVLIVMIYKIVATALTTGSGAVGGIFTPTLFVGAAIGFLFGDMAHAL 360 +V + W + K+V T + GSG GG TP ++GA +G +AL Sbjct: 287 IVEAFQQPLAPWD-----FLGKMVFTVTSLGSGFKGGEVTPLFYIGATLG-------NAL 334 Query: 361 WPHAISQPFAYAMVGMGAFLAAATNAPLMAILMIFEMTLSYQVVLPLMLSCVVAYVIA 418 P A +G A A A N PL + +M E+ Q+ L+CVVAY+ + Sbjct: 335 APLLHLPFPLLAGIGFVAVFAGAANTPLASTIMAIEL-FGPQIGPFAALACVVAYLFS 391 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.326 0.140 0.417 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,751,839 Number of extensions: 72688 Number of successful extensions: 345 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 335 Number of HSP's successfully gapped: 4 Length of query: 578 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 488 Effective length of database: 1,193,720 Effective search space: 582535360 Effective search space used: 582535360 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 133 (55.8 bits)