BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0005.0209.p (703 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311426|ref|YP_003775518.1| ATP-dependent Clp protease subu... 1263 0.0 gi|300310082|ref|YP_003774174.1| ClpA/B-type chaperone protein [... 468 e-133 gi|300313318|ref|YP_003777410.1| ClpA/B-type chaperone ATPase [H... 458 e-130 gi|300310731|ref|YP_003774823.1| ATP-dependent protease ATP-bind... 279 4e-76 >gi|300311426|ref|YP_003775518.1| ATP-dependent Clp protease subunit [Herbaspirillum seropedicae SmR1] Length = 861 Score = 1263 bits (3268), Expect = 0.0, Method: Compositional matrix adjust. Identities = 646/718 (89%), Positives = 671/718 (93%), Gaps = 19/718 (2%) Query: 1 VRGGASVSSQEGEGQREALKKYTLDLTERARNGKLDPVIGRDDEIRRAIQVLQRRSKNNP 60 VRGGASVSSQ+ EGQREALKKYTLDLTERAR GKLDPVIGRDDEIRRAIQVLQRRSKNNP Sbjct: 143 VRGGASVSSQQDEGQREALKKYTLDLTERARAGKLDPVIGRDDEIRRAIQVLQRRSKNNP 202 Query: 61 VLIGEPGVGKTAIVEGLAQRIVSGEVPDSLKSKRVLSLDMAALLAGAKYRGEFEERLKAV 120 VLIGEPGVGKTAIVEGLAQRIV+GEVPDSLKSKRVLSLDMAALLAGAKYRGEFEERLKAV Sbjct: 203 VLIGEPGVGKTAIVEGLAQRIVNGEVPDSLKSKRVLSLDMAALLAGAKYRGEFEERLKAV 262 Query: 121 LKEIAQDEGQTIVFIDELHTMVGAGKAEGAMDAGNMLKPALARGELHCVGATTLDEYRKY 180 LKEIAQDEGQTIVFIDELHTMVGAGKAEGAMDAGNMLKPALARGELHCVGATTLDEYR+Y Sbjct: 263 LKEIAQDEGQTIVFIDELHTMVGAGKAEGAMDAGNMLKPALARGELHCVGATTLDEYRQY 322 Query: 181 IEKDAALERRFQKIIVDEPSVEATIAILRGLQEKYEVHHGVDITDPAI------------ 228 IEKDAALERRFQKI+VDEPSVEATIAILRGLQEKYEVHHGVDITDPAI Sbjct: 323 IEKDAALERRFQKILVDEPSVEATIAILRGLQEKYEVHHGVDITDPAIVAAAELSHRYIT 382 Query: 229 ------XAIDLIDEAAAKIKIEIDSKPEVMDKLDRRLIQLKIEREAVKREKDEASQKRLT 282 AIDLIDEAA+KIKIEIDSKPEVMDKLDRRLIQLKIEREAVKREKDEASQKRL Sbjct: 383 DRFLPDKAIDLIDEAASKIKIEIDSKPEVMDKLDRRLIQLKIEREAVKREKDEASQKRLQ 442 Query: 283 LIEEEIIKLEREYADLEEIWKAEKSTVQGSQHLKEEIEQIRLQMDDATRKSDWQKVSELK 342 LIEEEI KLEREYADLEEIWKAEKS+ QG Q LKEEIE++RLQM++ATRKSDWQKVSELK Sbjct: 443 LIEEEIEKLEREYADLEEIWKAEKSSAQGGQQLKEEIEKVRLQMEEATRKSDWQKVSELK 502 Query: 343 YGKLAELEKALETQNKQDAASEQTVGKPRLVRTQVGAEEIAEIVARATGIPVSRMMQGER 402 YGKLAELE ALE QNK+DAA +T KP+LVRTQVGAEEIAEIVARATGIPVSRMMQGER Sbjct: 503 YGKLAELEAALEVQNKKDAAGVKT-EKPKLVRTQVGAEEIAEIVARATGIPVSRMMQGER 561 Query: 403 EKLLHMEEVLHDRVVGQDEAIVAVSDAIRRSRAGLGDPGKPYGSFMFLGPTGVGKTELCK 462 EKLLHME+VLH+RVVGQDEAIVAVSDAIRRSRAGLGDP KPYGSFMFLGPTGVGKTELCK Sbjct: 562 EKLLHMEDVLHERVVGQDEAIVAVSDAIRRSRAGLGDPSKPYGSFMFLGPTGVGKTELCK 621 Query: 463 ALASYLFDTDEAMIRIDMSEFMEKHSVARLIGAPPGYVGYEEGGYLTEAVRRKPYSVILL 522 ALASYLFDT+EAMIRIDMSEFMEKHSVARLIGAPPGYVGYEEGGYLTEAVRRKPYSVILL Sbjct: 622 ALASYLFDTEEAMIRIDMSEFMEKHSVARLIGAPPGYVGYEEGGYLTEAVRRKPYSVILL 681 Query: 523 DEIEKAHPDVFNVLLQVLDDGRMTDGQGRTVDFKNTVIVMTSNLGSHKIQAMEGSDAAVV 582 DEIEKAHPDVFNVLLQVLDDGRMTDGQGRTVDFKNTVIVMTSNLGSHKIQ+ME SD A+V Sbjct: 682 DEIEKAHPDVFNVLLQVLDDGRMTDGQGRTVDFKNTVIVMTSNLGSHKIQSMEDSDPALV 741 Query: 583 KMAVMAEVKLHFRPEFINRVDEIVVFHALDEKNIGAIAKIQLHILEARLAKMDIGLVITD 642 K+AVMAEV+ HFRPEFINRVDEIVVFHALD KNIGAIAKIQL ILE RLAK++IGL I++ Sbjct: 742 KLAVMAEVRTHFRPEFINRVDEIVVFHALDAKNIGAIAKIQLRILEQRLAKLEIGLEISE 801 Query: 643 DGLQKIAEAGFDPVYGARPLKRAIQQEIENPLSKLILQGKFGPKDVVHVSVKNGALSF 700 GLQKIAEAGFDPVYGARPLKRAIQQEIENPLSK IL GKFGPKDVV V V +G LSF Sbjct: 802 AGLQKIAEAGFDPVYGARPLKRAIQQEIENPLSKQILAGKFGPKDVVRVDVDHGQLSF 859 >gi|300310082|ref|YP_003774174.1| ClpA/B-type chaperone protein [Herbaspirillum seropedicae SmR1] Length = 893 Score = 468 bits (1203), Expect = e-133, Method: Compositional matrix adjust. Identities = 278/659 (42%), Positives = 403/659 (61%), Gaps = 45/659 (6%) Query: 2 RGGASVSSQEGEGQREALKKYTLDLTERARNGKLDPVIGRDDEIRRAIQVLQRRSKNNPV 61 +GG V G+ AL +YT +LT+RAR GK+DPV+GRD EIR+A+ +L RR +NNP+ Sbjct: 179 QGGDPVGEMRAGGRTPALDQYTTNLTQRAREGKIDPVVGRDGEIRQAVDILLRRRQNNPI 238 Query: 62 LIGEPGVGKTAIVEGLAQRIVSGEVPDSLKSKRVLSLDMAALLAGAKYRGEFEERLKAVL 121 L GE GVGKTA+VEGLA RI +VP +L+ + +LDM L AGA +GEFE RLK V+ Sbjct: 239 LTGEAGVGKTAVVEGLALRIAQDDVPQALQGVEIHTLDMGLLQAGASVKGEFENRLKGVI 298 Query: 122 KEIAQDEGQTIVFIDELHTMVGAGKAEGAMDAGNMLKPALARGELHCVGATTLDEYRKYI 181 E+ + I+FIDE HTM+GAG G DA N+LKPALARGEL + ATT EY+KY Sbjct: 299 DEVKKSPHPIILFIDEAHTMIGAGGQAGQNDAANLLKPALARGELRTIAATTWSEYKKYF 358 Query: 182 EKDAALERRFQKIIVDEPSVEATIAILRGLQEKYEVHHGVDITDPAIX------------ 229 EKDAAL RRFQ I V+EP+ E A+LR + E H GV + D AI Sbjct: 359 EKDAALARRFQVIKVEEPTEEVACAMLRAMAPLMEKHFGVRVYDEAITEAVRLSHRYISG 418 Query: 230 ------AIDLIDEAAAKIKIEIDSKPEVMDKLDRRLIQLKIEREAVKREKDEASQ--KRL 281 AI ++D A AK+ + ++ P ++ + RRL +L E A++RE + RL Sbjct: 419 RQLPDKAISVLDTACAKVALGQNATPARLEGVTRRLERLAAEIAALERETRSGASHATRL 478 Query: 282 TLIEEEIIKLEREYADLEEIWKAEKSTVQGSQHLKEEIEQIRLQMDDATRKSDWQKVSEL 341 ++ + + E +L++ W+ E+ L I+ R +++A ++S + S Sbjct: 479 GELQAQHAQAIAEQTELQQRWEQER-------ELNGRIQAARAALEEANQESASSQDSAA 531 Query: 342 KYGKLAELEKALETQNKQDAASEQTVGKPRLVRTQVGAEEIAEIVARATGIPVSRMMQGE 401 +L +L L+ G+ +V QV +AEIVA TGIP+ +M++ E Sbjct: 532 ARSELQQLLAQLKEHQ----------GETPMVPVQVDGHVVAEIVAGWTGIPLGKMVKDE 581 Query: 402 REKLLHMEEVLHDRVVGQDEAIVAVSDAIRRSRAGLGDPGKPYGSFMFLGPTGVGKTELC 461 + +L ++ +L +RV+GQ +A AV+ +R SRA L DPGKP G F+F+GP+GVGKTE Sbjct: 582 IKTVLGLDALLKERVLGQPQATAAVAQRVRTSRANLDDPGKPKGVFLFVGPSGVGKTETA 641 Query: 462 KALASYLFDTDEAMIRIDMSEFMEKHSVARLIGAPPGYVGYEEGGYLTEAVRRKPYSVIL 521 ALA L+ + +I I+MSE+ E H+V+ L G+PPGYVGY EGG LTEAVRR PYSV+L Sbjct: 642 LALADVLYGGERKLITINMSEYQEAHTVSGLKGSPPGYVGYGEGGVLTEAVRRNPYSVVL 701 Query: 522 LDEIEKAHPDVFNVLLQVLDDGRMTDGQGRTVDFKNTVIVMTSNLGSHKI-QAMEGSDAA 580 LDE+EKAHPDV + QV D G M DG+GR +DFKNT+I++TSN+ S ++ QA AA Sbjct: 702 LDEVEKAHPDVLELFFQVFDKGVMDDGEGREIDFKNTIIILTSNVASSQLMQACLNKSAA 761 Query: 581 VV----KMAVMAEVKL--HFRPEFINRVDEIVVFHALDEKNIGAIAKIQLHILEARLAK 633 + ++ V+ +L F+P F+ R+ +++ ++ + ++ + I K++L + R+A+ Sbjct: 762 ELPTPDELDVLIRPQLVKAFKPAFLGRL-KVIPYYPISDEVLEQIIKLKLGRIANRIAE 819 >gi|300313318|ref|YP_003777410.1| ClpA/B-type chaperone ATPase [Herbaspirillum seropedicae SmR1] Length = 889 Score = 458 bits (1179), Expect = e-130, Method: Compositional matrix adjust. Identities = 280/649 (43%), Positives = 395/649 (60%), Gaps = 53/649 (8%) Query: 14 GQREALKKYTLDLTERARNGKLDPVIGRDDEIRRAIQVLQRRSKNNPVLIGEPGVGKTAI 73 G+ AL +YT LT+RAR+GK+DPVIGRD EIR+A+ +L RR +NNP+L GE GVGKTA+ Sbjct: 187 GKMTALDQYTTQLTQRARDGKIDPVIGRDAEIRQAVDILLRRRQNNPILTGEAGVGKTAV 246 Query: 74 VEGLAQRIVSGEVPDSLKSKRVLSLDMAALLAGAKYRGEFEERLKAVLKEIAQDEGQTIV 133 VEGLA RI G+VP L+ + +LDM L AGA +GEFE RLK V+ E+ + I+ Sbjct: 247 VEGLALRIAQGDVPKPLQGVEIHTLDMGLLQAGASVKGEFESRLKTVIDEVKKSAYPIIL 306 Query: 134 FIDELHTMVGAGKAEGAMDAGNMLKPALARGELHCVGATTLDEYRKYIEKDAALERRFQK 193 FIDE HTM+GA G DA N+LKPALARGEL + ATT EY+KY EKDAAL RRFQ Sbjct: 307 FIDEAHTMIGAAGQAGQSDAANLLKPALARGELRTIAATTWSEYKKYFEKDAALARRFQV 366 Query: 194 IIVDEPSVEATIAILRGLQEKYEVHHGVDITDPAI------------------XAIDLID 235 I V+EP + A+LR + E H GV + D A+ AI ++D Sbjct: 367 IKVEEPGEDVACAMLRAMVPLMEQHFGVRVYDEAVVEAVRLSHRYISGRQLPDKAISVLD 426 Query: 236 EAAAKIKIEIDSKPEVMDKLDRRLIQLKIEREAVKREKDEASQ--KRLTLIEEEIIKLER 293 A AK+ + D+ P +++ + RRL +L E A++RE SQ +RL + ++ + Sbjct: 427 TACAKVALGRDATPALLEGVTRRLERLDTEISALRREAASGSQHEERLASLLDQHVCAVA 486 Query: 294 EYADLEEIWKAEKSTVQGSQHLKEEIEQIRLQMDD-ATRKSDWQKVSELKYGKLAELEKA 352 E DL+ W+ E+ L E I+Q R +++ T + V + AEL++ Sbjct: 487 ERHDLQLRWEQER-------ELNERIQQARATLENPGTGDARGGNVDAAR----AELKQL 535 Query: 353 L-ETQNKQDAASEQTVGKPRLVRTQVGAEEIAEIVARATGIPVSRMMQGEREKLLHMEEV 411 L E + +Q G +V QV +AEIVA TGIP+ +M++ E + +L ++ + Sbjct: 536 LDELRQRQ--------GDAPMVPIQVDGHVVAEIVAAWTGIPLGKMVKDEIQTVLDLDAL 587 Query: 412 LHDRVVGQDEAIVAVSDAIRRSRAGLGDPGKPYGSFMFLGPTGVGKTELCKALASYLFDT 471 L +RV+GQ +A AV+ +R SRA L DPGKP G F+F+GP+GVGKTE ALA L+ Sbjct: 588 LQERVLGQPQATSAVAQRVRTSRANLDDPGKPKGVFLFVGPSGVGKTETALALADVLYGG 647 Query: 472 DEAMIRIDMSEFMEKHSVARLIGAPPGYVGYEEGGYLTEAVRRKPYSVILLDEIEKAHPD 531 + +I I+MSE+ E H+V+ L G+PPGYVGY EGG LTEAVRR PYSV+LLDE+EKAH D Sbjct: 648 ERKLITINMSEYQEAHTVSGLKGSPPGYVGYGEGGVLTEAVRRNPYSVVLLDEVEKAHAD 707 Query: 532 VFNVLLQVLDDGRMTDGQGRTVDFKNTVIVMTSNLGSHK-IQAM------EGSDAAVVKM 584 V + QV D G M D +GR +DFKNT+I++TSN+ S + +QA E + Sbjct: 708 VLELFFQVFDKGVMDDAEGREIDFKNTIIILTSNVASAQMMQACLNKSQEELPTPEELDT 767 Query: 585 AVMAEVKLHFRPEFINRVDEIVVFHALDEKNIGAIAKIQLHILEARLAK 633 + ++ F+P F+ R+ ++V ++ + + + I K++L AR+AK Sbjct: 768 LIRPQLIKTFKPAFLGRL-KVVPYYPIADVVLVEIIKLKL----ARIAK 811 >gi|300310731|ref|YP_003774823.1| ATP-dependent protease ATP-binding specificity subunit [Herbaspirillum seropedicae SmR1] Length = 767 Score = 279 bits (714), Expect = 4e-76, Method: Compositional matrix adjust. Identities = 151/328 (46%), Positives = 212/328 (64%), Gaps = 13/328 (3%) Query: 361 AASEQTVGKPRLVRTQVGAEEIAEIVARATGIPVSRMMQGEREKLLHMEEVLHDRVVGQD 420 A+++ + K + +T +G EI EI+++ IP + Q +R KL ++ L + V GQD Sbjct: 409 GAAQRILPKSKQKKT-IGKPEIEEIISKIARIPPQSVNQDDRAKLQTIDRDLKNVVFGQD 467 Query: 421 EAIVAVSDAIRRSRAGLGDPGKPYGSFMFLGPTGVGKTELCKALASYLFDTDEAMIRIDM 480 AI A+S AI+ +RAGLG KP GSF+F GPTGVGKTE+ K LA F ++R DM Sbjct: 468 PAIEALSSAIKMARAGLGKTDKPIGSFLFSGPTGVGKTEVAKQLA---FILGIELVRFDM 524 Query: 481 SEFMEKHSVARLIGAPPGYVGYEEGGYLTEAVRRKPYSVILLDEIEKAHPDVFNVLLQVL 540 SE+ME+H+V+RLIGAPPGYVG+++GG LTEAV +KP++V+LLDEIEKAHPD+FN+LLQV+ Sbjct: 525 SEYMERHAVSRLIGAPPGYVGFDQGGLLTEAVNKKPHAVLLLDEIEKAHPDIFNILLQVM 584 Query: 541 DDGRMTDGQGRTVDFKNTVIVMTSNLGSHKIQA-----MEGSDAAVVKMAVMAEVKLHFR 595 D G +TD GR DF+N +I+MT+N G+ +Q E +A MA++K F Sbjct: 585 DHGTLTDNNGRKTDFRNVIIIMTTNAGAESLQKRTIGFTEKKEAG----DEMADIKRMFT 640 Query: 596 PEFINRVDEIVVFHALDEKNIGAIAKIQLHILEARLAKMDIGLVITDDGLQKIAEAGFDP 655 PEF NR+D I+ F LDE+ I + L LE +L + + V +D + + + GFDP Sbjct: 641 PEFRNRLDAIISFRPLDEEIILRVVDKFLMQLEEQLHEKKVEAVFSDSLRKFLGKKGFDP 700 Query: 656 VYGARPLKRAIQQEIENPLSKLILQGKF 683 + GARP+ R IQ I L+ +L GK Sbjct: 701 LMGARPMSRLIQDLIRKALADELLFGKL 728 Score = 263 bits (673), Expect = 3e-71, Method: Compositional matrix adjust. Identities = 139/264 (52%), Positives = 179/264 (67%), Gaps = 20/264 (7%) Query: 4 GASVSSQEGEGQREALKKYTLDLTERARNGKLDPVIGRDDEIRRAIQVLQRRSKNNPVLI 63 GA EG+ + L ++T +L + A GK+DP+IGR+ E+ R IQ L RR KNNP+L+ Sbjct: 157 GAEDVQAEGQQKESPLDQFTQNLNKAAAEGKIDPLIGRESEVERVIQTLCRRRKNNPLLV 216 Query: 64 GEPGVGKTAIVEGLAQRIVSGEVPDSLKSKRVLSLDMAALLAGAKYRGEFEERLKAVLKE 123 GE GVGKTAI EGLA R+ G+VP+ LK+ V SLDM ALLAG KYRG+FE+RLKAVLK+ Sbjct: 217 GEAGVGKTAIAEGLAWRVTQGDVPEILKNAVVYSLDMGALLAGTKYRGDFEQRLKAVLKQ 276 Query: 124 IAQDEGQTIVFIDELHTMVGAGKAE-GAMDAGNMLKPALARGELHCVGATTLDEYRKYIE 182 + +D I+FIDE+HT++GAG A G +DA N+LKPAL+ G+L C+GATT E+R E Sbjct: 277 L-KDSPNGILFIDEIHTIIGAGSASGGTLDASNLLKPALSSGQLKCIGATTYTEFRGVFE 335 Query: 183 KDAALERRFQKIIVDEPSVEATIAILRGLQEKYEVHHGVDITDPAIX------------- 229 KD AL RRFQKI V+EP+VE T+ ILRGL+ K+E HHGV + A+ Sbjct: 336 KDHALSRRFQKIDVNEPTVEQTVQILRGLKSKFEEHHGVKYSASALTSAAELAARFINDR 395 Query: 230 -----AIDLIDEAAAKIKIEIDSK 248 AID+IDEA A +I SK Sbjct: 396 HLPDKAIDVIDEAGAAQRILPKSK 419 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.316 0.134 0.366 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,882,253 Number of extensions: 74900 Number of successful extensions: 378 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 366 Number of HSP's successfully gapped: 5 Length of query: 703 Length of database: 1,619,870 Length adjustment: 92 Effective length of query: 611 Effective length of database: 1,184,250 Effective search space: 723576750 Effective search space used: 723576750 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 134 (56.2 bits)