BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0005.0203.p (400 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311423|ref|YP_003775515.1| NodT family protein [Herbaspiri... 537 e-154 gi|300312564|ref|YP_003776656.1| outer membrane multidrug resist... 211 8e-56 gi|300312015|ref|YP_003776107.1| NodT family protein [Herbaspiri... 198 6e-52 gi|300313898|ref|YP_003777990.1| outer membrane drug efflux lipo... 131 9e-32 gi|300310691|ref|YP_003774783.1| outer membrane drug efflux lipo... 115 9e-27 >gi|300311423|ref|YP_003775515.1| NodT family protein [Herbaspirillum seropedicae SmR1] Length = 487 Score = 537 bits (1384), Expect = e-154, Method: Compositional matrix adjust. Identities = 301/390 (77%), Positives = 341/390 (87%), Gaps = 4/390 (1%) Query: 6 AMAVVAHAGELPQAQLDAAVGRKDWSDNVYYGASFRDKLTWNNTAVLSLSYNLDLWDRNR 65 +MAVVAHAGELP AQLD +VGRKDWSDNVYYGA+FR+KLTWNNTA L LSYN+DLWDRN+ Sbjct: 97 SMAVVAHAGELPSAQLDVSVGRKDWSDNVYYGAAFRNKLTWNNTATLGLSYNVDLWDRNK 156 Query: 66 NTTERAQDEIHAVAADARAAQLELESNVVRTYVQLSLQYGLLDSAQDTLKQEQQILDLAN 125 N T RAQDE+HAVAADARAAQL+LE NVVRTYVQL+LQYGLLD+ + LK+EQ+ILDLA+ Sbjct: 157 NATLRAQDEMHAVAADARAAQLDLEGNVVRTYVQLALQYGLLDNTEALLKEEQKILDLAH 216 Query: 126 RRFRGGIGTQMEITQAEVPLPETRRQIEVFNENIALTRNQLAALLGEGPGAGDNITRPVM 185 RRF+GG+GTQ+EITQAE PLPETRRQIE +ENIAL RNQLAALLG GPGA D +TRP M Sbjct: 217 RRFKGGLGTQLEITQAESPLPETRRQIEALHENIALIRNQLAALLGAGPGAADGVTRPGM 276 Query: 186 NLQAALGLPSALPAELIGRRPDISAX----EASAKGIAVAKAAFYPNVNLLASIGPTAAG 241 LQ +LGLPSALPA+L+GRRPDISA EA+AKGI+VAKAAFYPN+NLLA IGP AAG Sbjct: 277 QLQTSLGLPSALPADLVGRRPDISAARWRVEAAAKGISVAKAAFYPNINLLAGIGPAAAG 336 Query: 242 GGLLSFLSARNITTTYGPAISLPIFEGGRLRGQLGATTAGYDMEVEHYNLLLSQALKSIA 301 GGL SFLSA N+ TTYGPAISLPIFEGGRLRGQLGA +AGYD+EVEHYN LLSQALK I+ Sbjct: 337 GGLFSFLSAPNLQTTYGPAISLPIFEGGRLRGQLGAASAGYDIEVEHYNALLSQALKGIS 396 Query: 302 DQIVTLQAIQRQQVQADLSVAAAQKNIDIATRAYQRGLTNYVNVLTAQTQWQHQQQVVQQ 361 DQ+VTLQ++ RQQ QA SV AAQKN+DIATRAYQRGLT+Y+NVL AQTQW QQQ+VQQ Sbjct: 397 DQVVTLQSVARQQEQAQQSVQAAQKNVDIATRAYQRGLTDYLNVLHAQTQWLRQQQIVQQ 456 Query: 362 LLAQKLTAYASLTVALGGGLEVSAPETEAA 391 L AQ+L AYA+L ALGGG+E +AP AA Sbjct: 457 LQAQRLAAYAALQTALGGGIEEAAPAKVAA 486 >gi|300312564|ref|YP_003776656.1| outer membrane multidrug resistance lipoprotein [Herbaspirillum seropedicae SmR1] Length = 515 Score = 211 bits (537), Expect = 8e-56, Method: Compositional matrix adjust. Identities = 140/370 (37%), Positives = 202/370 (54%), Gaps = 9/370 (2%) Query: 17 PQAQLDAAVGRKDWSDNVYYGASFRDKLTWNNT--AVLSLSYNLDLWDRNRNTTERAQDE 74 PQ +A++ R S N F K +NN A L SY +D W +NR A E Sbjct: 125 PQVSANASLDRMKQSYNNGVPPDFVPK-DYNNATRATLDFSYEIDFWGKNRAALAAATSE 183 Query: 75 IHAVAADARAAQLELESNVVRTYVQLSLQYGLLDSAQDTLKQEQQILDLANRRFRGGIGT 134 + A ADA A++ L +++ Y +L+ Y D+ + LK + LDL N+RF G+ T Sbjct: 184 LEASRADAAQARITLATSIASAYAELAQLYAQRDTNEAALKVRVESLDLFNQRFTNGLET 243 Query: 135 QMEITQAEVPLPETRRQIEVFNENIALTRNQLAALLGEGPGAGDNITRPVMNLQAALGLP 194 + + Q E + ++ +E+I L RN+LAALLG GP G +TRP ++L LP Sbjct: 244 RGSVKQMEARRAIAQADLKATDESIGLQRNKLAALLGAGPDRGLQLTRPQIDLSRPFALP 303 Query: 195 SALPAELIGRRPDISA----XEASAKGIAVAKAAFYPNVNLLASIGPTAAGGGLLSFLSA 250 + LP EL+GRRPDI A EA+ K I VA+AAFYPNVNL A G + G +L+ A Sbjct: 304 AQLPVELLGRRPDIVAARLRAEAAGKQIKVARAAFYPNVNLTAYFGFQSLGIDMLT--RA 361 Query: 251 RNITTTYGPAISLPIFEGGRLRGQLGATTAGYDMEVEHYNLLLSQALKSIADQIVTLQAI 310 + + GPAISLPIF GGRLRGQ + +A YD V +Y+ ++QAL+ +AD V+ +A+ Sbjct: 362 GSDIGSIGPAISLPIFNGGRLRGQFRSASASYDEAVANYDQAVTQALQDVADVGVSEKAL 421 Query: 311 QRQQVQADLSVAAAQKNIDIATRAYQRGLTNYVNVLTAQTQWQHQQQVVQQLLAQKLTAY 370 + + AA++ I + Y GL Y++VL AQ + + L ++ T Sbjct: 422 AGRLADVQAAADAAEEAYRIVSNRYNGGLATYLDVLNAQDTLISNLRQLSDLRSRMFTLD 481 Query: 371 ASLTVALGGG 380 +L ALGGG Sbjct: 482 VALVRALGGG 491 >gi|300312015|ref|YP_003776107.1| NodT family protein [Herbaspirillum seropedicae SmR1] Length = 495 Score = 198 bits (504), Expect = 6e-52, Method: Compositional matrix adjust. Identities = 132/373 (35%), Positives = 194/373 (52%), Gaps = 10/373 (2%) Query: 16 LPQAQLDAAVGRKDWSDNVYYGASFRDKLTWNNTAVLSLSYNLDLWDRNRNTTERAQDEI 75 LP +A+V R +S Y + A L++ Y LDLW++N+ +A Sbjct: 115 LPSVNAEASVTRNQFSSTTIYPPPYGGNWYNEKKAGLNVGYELDLWNKNQAALAQAISSE 174 Query: 76 HAVAADARAAQLELESNVVRTYVQLSLQYGLLDSAQDTLKQEQQILDLANRRFRGGIGTQ 135 A A + A+L L +++V Y QL+ QY L D Q T+ Q + + R R G+ +Q Sbjct: 175 KAAQASEQEARLALTASIVTVYSQLAAQYALHDILQSTVDQRTSLEKITAERLRTGLDSQ 234 Query: 136 MEITQAEVPLPETRRQIEVFNENIALTRNQLAALLGEGPGAGDNITRPVMNLQAALGLPS 195 +E Q+ + R Q+ I L R QL AL G+GP G + P + A GLP+ Sbjct: 235 IERDQSRTSSADARAQLAQSEGQIVLLRQQLGALAGKGPDYGLQLAPPALQGLATPGLPA 294 Query: 196 ALPAELIGRRPDISAX----EASAKGIAVAKAAFYPNVNLLASIGPTAAGGGLLSF--LS 249 LP L+GRRPDI A EA+++G+ VAKA FYP++NL A IG LL + Sbjct: 295 ELPLNLMGRRPDIVAARWQVEAASRGVDVAKARFYPDINLSAMIGFDT----LLDSNPFT 350 Query: 250 ARNITTTYGPAISLPIFEGGRLRGQLGATTAGYDMEVEHYNLLLSQALKSIADQIVTLQA 309 A + + +GPAI+LPIFEGG LR L A Y++ V YN L+ A +A QI + A Sbjct: 351 AASKSIAFGPAITLPIFEGGALRAGLKGEYASYELAVATYNKTLNDAYADVARQIAAIHA 410 Query: 310 IQRQQVQADLSVAAAQKNIDIATRAYQRGLTNYVNVLTAQTQWQHQQQVVQQLLAQKLTA 369 +RQ ++ AA++ +A Y+ GL + + +L+AQT Q+Q + L AQ+ Sbjct: 411 TERQLPIRSEALQAAERAYALARERYRLGLVSQLTLLSAQTGVLAQRQAMVALQAQRRDQ 470 Query: 370 YASLTVALGGGLE 382 +L ALGGG + Sbjct: 471 QVALYKALGGGFD 483 >gi|300313898|ref|YP_003777990.1| outer membrane drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 486 Score = 131 bits (330), Expect = 9e-32, Method: Compositional matrix adjust. Identities = 119/399 (29%), Positives = 193/399 (48%), Gaps = 17/399 (4%) Query: 3 QAQAMAVVAHAGELPQAQLDAAVGRKDWSDNV----YYGASFRDKLTWNNTAVL--SLSY 56 QA+A V A + PQ L+A R S N Y +F T N L S+SY Sbjct: 88 QARAQLNVTGAAQTPQVNLNARAARSRISANRPLTNYNSPNFS---TVQNDFALGASVSY 144 Query: 57 NLDLWDRNRNTTERAQDEIHAVAADARAAQLELESNVVRTYVQLSLQYGLLDSAQDTLKQ 116 +D + R + + E A AAD +L L + + Y L LD + + Sbjct: 145 EVDFFGRVQRSVEAAGAAAEQSAADLENTRLLLTAELATNYFNLRELDVELDVVRRAIAL 204 Query: 117 EQQILDLANRRFRGGIGTQMEITQAEVPLPETRRQIEVFNENIALTRNQLAALLGEGPGA 176 ++Q L+LA R G + +++ Q + L T QI++ A + +A L G P Sbjct: 205 QRQALELATARHDLGATSGLDMAQQQALLDSTLTQIDLLGRQRAQYEHAIATLTGT-PAP 263 Query: 177 GDNITRPVMNLQAALGLPSALPAELIGRRPDISAXEASAKG----IAVAKAAFYPNVNLL 232 +IT P + +P +P++++ RRPDI+A E + I VA AA+YP+ L Sbjct: 264 AFSIT-PSLTPITPPAIPVGVPSDVLERRPDIAAAERAMAAANAQIGVASAAYYPSFMLQ 322 Query: 233 ASIGPTAAGGGLLSFLSARNITTTYGPAISLPIFEGGRLRGQLGATTAGYDMEVEHYNLL 292 S G + L +A ++ + G + S +F+GGRLR + + AGY+ V Y Sbjct: 323 PSYGVDSRNWATL--FNAPSLLWSLGVSASQSLFDGGRLRAGVDFSQAGYEATVASYRRT 380 Query: 293 LSQALKSIADQIVTLQAIQRQQVQADLSVAAAQKNIDIATRAYQRGLTNYVNVLTAQTQW 352 + A++ + D I L A++R Q+ L++A+A++ ++IA+ Y+ G T Y++V+TAQ Sbjct: 381 VLTAMQEVEDGITGLAALERAYAQSQLAIASARRVLEIASSRYEGGATPYLDVITAQQSL 440 Query: 353 QHQQQVVQQLLAQKLTAYASLTVALGGGLEVSAPETEAA 391 + ++ QL+ Q+L L ALGG + P EAA Sbjct: 441 LNSERQAAQLMGQRLLVSVFLIKALGGDWQSRTPPAEAA 479 >gi|300310691|ref|YP_003774783.1| outer membrane drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 498 Score = 115 bits (287), Expect = 9e-27, Method: Compositional matrix adjust. Identities = 99/342 (28%), Positives = 158/342 (46%), Gaps = 13/342 (3%) Query: 49 TAVLSLS-YNLDLWDRNRNTTERAQDEIHAVAADARAAQLELESNVVRTYVQLSLQYGLL 107 TA L S Y LDL+ R R+ E+A + A R+ Q+ L + V ++ L+ L Sbjct: 142 TATLGFSAYELDLFGRVRSLKEQALQSFLSTAEARRSTQISLIAEVATAWLTLASDQDRL 201 Query: 108 DSAQDTLKQEQQILDLANRRFRGGIGTQMEITQAEVPLPETRRQIEVFNENIALTRNQLA 167 AQDTL+ + L R F G + + + QA+ + R +E + + RN L Sbjct: 202 RLAQDTLESQSSSYALTQRSFELGSSSALTLRQAQTSVESARVDVESYTAQVDQDRNALV 261 Query: 168 ALLGEG-PGAGDNITRPVMNLQAALGL---PSALPAELIGRRPDISAXE----ASAKGIA 219 L+G+ P A P L AA L P LP++L+ RR DI E A+ I Sbjct: 262 LLVGKDLPTALLPQGLPDTRLAAASPLATIPPGLPSDLLQRRADIVQAERDLRAANANIG 321 Query: 220 VAKAAFYPNVNLLASIGPTAAGGGLLSFLSARNITTTYGPAISLPIFEGGRLRGQLGATT 279 A+AAFYP++ L + G A L + + ++ P ISLPIF+GG + L T Sbjct: 322 AARAAFYPSITLTVNAG--TASARLAGLFKGGSGSWSFVPQISLPIFDGGANQANLDIAT 379 Query: 280 AGYDMEVEHYNLLLSQALKSIADQIVTLQAIQRQQVQADLSVAAAQKNIDIATRAYQRGL 339 D+ V Y + A + ++D + + RQ + V A+ + ++ Y G+ Sbjct: 380 VSRDISVAQYEKAIQTAFREVSDALAQRNTLGRQLQAQEALVQASDEAFRLSQARYHSGV 439 Query: 340 TNYVNVLTAQ-TQWQHQQQVVQQLLAQKLTAYASLTVALGGG 380 +Y+ VL +Q T + Q+ ++ L+ + T + LGGG Sbjct: 440 DSYLTVLDSQRTLYSAQKNLIGTQLS-RWTNLVTFYKTLGGG 480 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.315 0.129 0.355 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 971,869 Number of extensions: 34292 Number of successful extensions: 233 Number of sequences better than 1.0e-08: 17 Number of HSP's gapped: 197 Number of HSP's successfully gapped: 17 Length of query: 400 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 313 Effective length of database: 1,207,925 Effective search space: 378080525 Effective search space used: 378080525 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 132 (55.5 bits)