BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0005.0195.p (462 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311416|ref|YP_003775508.1| gluconate transporter family pr... 684 0.0 gi|300310424|ref|YP_003774516.1| high-affinity gluconate permeas... 344 8e-96 gi|300311081|ref|YP_003775173.1| high-affinity gluconate transpo... 327 9e-91 >gi|300311416|ref|YP_003775508.1| gluconate transporter family protein [Herbaspirillum seropedicae SmR1] Length = 456 Score = 684 bits (1765), Expect = 0.0, Method: Compositional matrix adjust. Identities = 376/459 (81%), Positives = 418/459 (91%), Gaps = 5/459 (1%) Query: 3 LTTEIAAWAVQDTRLLLSVLIALIAIVVLISVLKIAPFLSILLGTFIAGTGGPGXAGLPL 62 ++T +AAWA QDT+LLL L AL+ IVVLIS L IAPFLSIL+GTF+AG G AGLPL Sbjct: 2 ISTHLAAWAAQDTQLLLVTLTALVTIVVLISFLSIAPFLSILIGTFVAGIG----AGLPL 57 Query: 63 EKIASSFSKGAGSILGEAGIIIALGAMLGALMADSGAADKIVTTLLRYAKGAAIPWMMAL 122 E IA +FSKGAGS+LGEAGIIIALGAMLGALMA+SGAAD+IV TLLRYA+G+ IPWMMAL Sbjct: 58 EDIAKAFSKGAGSLLGEAGIIIALGAMLGALMAESGAADRIVNTLLRYARGSFIPWMMAL 117 Query: 123 VALVVGLPLFFEVGLVMMAPIIFVMARRSQQPILRVAIPALAGMTTLHALLPPHPGPLIA 182 VALVVGLPLFFEVGLVMMAPIIFVMARRS+ PI+RVAIPALAGMTTLHALLPPHPGPLIA Sbjct: 118 VALVVGLPLFFEVGLVMMAPIIFVMARRSELPIMRVAIPALAGMTTLHALLPPHPGPLIA 177 Query: 183 VSALHADLGTTMLLGFVVAIPAVILAGPLYGMWLAPRMPIGEPEQIGALFKGQQAPELQP 242 VSALHADLGTTMLLGF+VAIPAVI+AGPLYG ++APR+ + EP+QIG LF ++ + QP Sbjct: 178 VSALHADLGTTMLLGFIVAIPAVIIAGPLYGNFIAPRLNLAEPDQIGKLFSAREG-QSQP 236 Query: 243 GFLPSLITILLPVVLMLGRTVAKIWVMPETTLFEMLNFFGEPIIALTVTVLFAVVVLGWS 302 FL +L+TILLPVVLMLGRTVA+IWV P+T LFEMLNFFGEPIIALTVTVLFAVVVLGW Sbjct: 237 SFLVALVTILLPVVLMLGRTVARIWVTPKTELFEMLNFFGEPIIALTVTVLFAVVVLGWG 296 Query: 303 RGVSRTDVGAILRKALPPIAGLLLTIGAGGGLKQALLTAGISDTITKVATGTHVPLILLA 362 +G SR +VGA LR+ALPPIAGLLLTIGAGGGLKQ LL+AGISDTITK+A GTH+PLILLA Sbjct: 297 QGSSRFEVGATLRRALPPIAGLLLTIGAGGGLKQTLLSAGISDTITKIADGTHMPLILLA 356 Query: 363 WLIAVALRQATGSATVATTATAGIVAPLVASLSGTHNALMALAIGAGSVFFCHVNDAGFW 422 W+IAVALRQATGSATVATTATAGIVAPLVA LS THN+LMALAIGAGSVFFCHVNDAGFW Sbjct: 357 WIIAVALRQATGSATVATTATAGIVAPLVAGLSATHNSLMALAIGAGSVFFCHVNDAGFW 416 Query: 423 MVKEYFGLDLKQTFATWSVIQTIVSVVGLVMTLLLWGVL 461 MVKEYFGL+LKQT ATWSVIQT+VSV+GL MTL+LWG+L Sbjct: 417 MVKEYFGLNLKQTLATWSVIQTLVSVIGLGMTLVLWGLL 455 >gi|300310424|ref|YP_003774516.1| high-affinity gluconate permease [Herbaspirillum seropedicae SmR1] Length = 450 Score = 344 bits (883), Expect = 8e-96, Method: Compositional matrix adjust. Identities = 191/446 (42%), Positives = 297/446 (66%), Gaps = 6/446 (1%) Query: 13 QDTRLLLSVLIALIAIVVLISVLKIAPFLSILLGTFIAGTGGPGXAGLPLEKIASSFSKG 72 D LL + +IA++A++++I+ L+I PF+++ + + + G +G+PL KI SF G Sbjct: 6 HDATLLFNAVIAILALILMITRLRIHPFIALTITSGLLGL----ISGMPLPKIVKSFQDG 61 Query: 73 AGSILGEAGIIIALGAMLGALMADSGAADKIVTTLLRYAKGAAIPWMMALVALVVGLPLF 132 G +LG GI++ LG MLG LMA+SG AD+I TL+R + W M + A +VG+PLF Sbjct: 62 FGGVLGFVGIVLGLGTMLGKLMAESGGADQIAQTLVRAFGRERVHWAMMMGAFLVGIPLF 121 Query: 133 FEVGLVMMAPIIFVMARRSQQPILRVAIPALAGMTTLHALLPPHPGPLIAVSALHADLGT 192 FE+G V++ P++F++ARRS P+ ++ +P LA ++ +H L+PPHPGPL+A+ AD+G Sbjct: 122 FEIGFVLLIPLVFIVARRSGVPLFKIGMPMLASLSVMHGLVPPHPGPLLAIGIFGADIGK 181 Query: 193 TMLLGFVVAIPAVILAGPLYGMWLAPRMPIGEPEQIGA-LFKGQQAPELQPGFLPSLITI 251 T+ G +V +P VI+AGP++G +++ + I E++ A L + Q +L PGF +LIT+ Sbjct: 182 TIFYGLLVGLPTVIIAGPMFGAFISRYVDIKPSEELMAQLVREPQTTKL-PGFATTLITV 240 Query: 252 LLPVVLMLGRTVAKIWVMPETTLFEMLNFFGEPIIALTVTVLFAVVVLGWSRGVSRTDVG 311 L PV LML +T+ + + + ++++F G PI+AL +L A+ G +RG +R V Sbjct: 241 LAPVFLMLLKTLVDVTLPEGHAMRDLMDFIGNPIVALLAALLLAIWTFGIARGFTRQQVL 300 Query: 312 AILRKALPPIAGLLLTIGAGGGLKQALLTAGISDTITKVATGTHVPLILLAWLIAVALRQ 371 + ++L P A ++L IGAGGG KQ L+ +G+ I ++A + +LLAW +A +R Sbjct: 301 KFVDQSLAPTAAIVLIIGAGGGFKQMLVNSGVGTAIGQLAVHAQISPLLLAWFVAGVIRV 360 Query: 372 ATGSATVATTATAGIVAPLVASLSGTHNALMALAIGAGSVFFCHVNDAGFWMVKEYFGLD 431 ATGSATVAT AGIVAPLVA + GT+ L+ LA GAGS+ HVNDAGFW+VKEYF + Sbjct: 361 ATGSATVATITGAGIVAPLVALIPGTNKELLVLATGAGSLILSHVNDAGFWLVKEYFNMS 420 Query: 432 LKQTFATWSVIQTIVSVVGLVMTLLL 457 + +TF TW+V++T++SVV ++ +LL Sbjct: 421 VGETFKTWTVMETLISVVAIIFIMLL 446 >gi|300311081|ref|YP_003775173.1| high-affinity gluconate transporter (GNT-III system) transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 450 Score = 327 bits (839), Expect = 9e-91, Method: Compositional matrix adjust. Identities = 181/441 (41%), Positives = 275/441 (62%), Gaps = 9/441 (2%) Query: 11 AVQDTRLLLSVLIALIAIVVLISVLKIAPFLSILLGTFIAGTGGPGXAGLPLEKIASSFS 70 AVQ + LL+ L+A+IA++VLI+ K+ PF+ +++ + + G G+P+ I +F Sbjct: 3 AVQGSALLVYALVAVIALIVLIAKFKMNPFIVLIVVSLVLGLA----VGMPMGNIVKAFE 58 Query: 71 KGAGSILGEAGIIIALGAMLGALMADSGAADKIVTTLLRYAKGAAIPWMMALVALVVGLP 130 G G+ LG +++ LG MLG +MA+SG A++I T+++ + W M VA +VGLP Sbjct: 59 TGVGNALGHIALVVGLGTMLGKMMAESGGAERIANTMIKAFGEKNVHWAMMTVAFIVGLP 118 Query: 131 LFFEVGLVMMAPIIFVMARRSQQPILRVAIPALAGMTTLHALLPPHPGPLIAVSALHADL 190 +FFEVG V++ PI F +A+R+ ++ V IP +AG++ +H L+PPHP L+AV+A AD+ Sbjct: 119 VFFEVGFVLLVPIAFNVAKRTGTNMVLVGIPMVAGLSVVHGLIPPHPAALLAVTAYSADI 178 Query: 191 GTTMLLGFVVAIPAVILAGPLYGMWLAPRMPIGEPEQ-IGALF--KGQQAPELQPGFLPS 247 G T+L +V IP I+AGP++G L ++ I P+ + + F +G++ EL PGF + Sbjct: 179 GRTILYALIVGIPTAIIAGPIFGK-LISKVVIPNPDNPLISQFVDEGKKDREL-PGFGIT 236 Query: 248 LITILLPVVLMLGRTVAKIWVMPETTLFEMLNFFGEPIIALTVTVLFAVVVLGWSRGVSR 307 L TILLPV LML + A ++ P+T + L G +IAL + L + G +RG Sbjct: 237 LFTILLPVALMLIGSWADLFFAPKTFANDFLRLIGNSVIALLIATLVSFWTFGRARGFGA 296 Query: 308 TDVGAILRKALPPIAGLLLTIGAGGGLKQALLTAGISDTITKVATGTHVPLILLAWLIAV 367 + + L PIA + L +GAG G + L+ G+S I +AT H+ ++L W +A Sbjct: 297 DQILKFTNECLAPIASITLVVGAGAGFGRILMDGGVSKAIVGIATDAHLSPLILGWFVAA 356 Query: 368 ALRQATGSATVATTATAGIVAPLVASLSGTHNALMALAIGAGSVFFCHVNDAGFWMVKEY 427 +R ATGSATVA T GIVAP+V+++ G LM LA GAGS+ HVND GFW+VKEY Sbjct: 357 LIRVATGSATVAMTTACGIVAPIVSTVGGVRPELMVLATGAGSLILSHVNDGGFWLVKEY 416 Query: 428 FGLDLKQTFATWSVIQTIVSV 448 F + + QTF TW+V++T+VSV Sbjct: 417 FNMTVPQTFKTWTVMETLVSV 437 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.326 0.140 0.417 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,022,321 Number of extensions: 37006 Number of successful extensions: 204 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 200 Number of HSP's successfully gapped: 3 Length of query: 462 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 373 Effective length of database: 1,198,455 Effective search space: 447023715 Effective search space used: 447023715 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 132 (55.5 bits)