BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0005.0186.p (320 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312339|ref|YP_003776431.1| branched-chain amino acid ABC t... 294 7e-81 gi|300314071|ref|YP_003778163.1| amino acid ABC transporter peri... 253 1e-68 gi|300309490|ref|YP_003773582.1| branched-chain amino acid ABC t... 199 3e-52 gi|300312372|ref|YP_003776464.1| branched-chain amino acid ABC t... 153 2e-38 >gi|300312339|ref|YP_003776431.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 404 Score = 294 bits (752), Expect = 7e-81, Method: Compositional matrix adjust. Identities = 140/159 (88%), Positives = 149/159 (93%) Query: 84 TFLLQAQGSKAQILGLANAGDDTVNAIKAAKEFGVTKTMKLVGLLMVINDIHALGLNNAE 143 +FLLQAQ SKAQILGLANAG+DTVN+IKAAKEFGVTKTMKLVGLLMVINDIHALGL NAE Sbjct: 214 SFLLQAQASKAQILGLANAGEDTVNSIKAAKEFGVTKTMKLVGLLMVINDIHALGLANAE 273 Query: 144 GLMMTDSWYWDKDDASRQFANRFFLKMKKMPSTHQAAAYSATTTYLKAVAAIKTDDSTKV 203 GLMMTDSWYWDKD+ SR+FA RFF KMKKMPSTHQAAAYSATTTYLKAV AI TDDSTKV Sbjct: 274 GLMMTDSWYWDKDEPSRKFAERFFQKMKKMPSTHQAAAYSATTTYLKAVQAIGTDDSTKV 333 Query: 204 MAELKKMKIDDFYNKGYIRADGRNIHDMYLYQVKSPXDA 242 MA+LKK +IDDFYNKG IRADGRNIHDMYLYQVKSP ++ Sbjct: 334 MAQLKKTRIDDFYNKGSIRADGRNIHDMYLYQVKSPAES 372 Score = 139 bits (349), Expect = 4e-34, Method: Compositional matrix adjust. Identities = 66/73 (90%), Positives = 71/73 (97%) Query: 1 VLGKPIEVLSADHLNKADIAATKAREWADQRGLDMLIGGVNSATALATNKVMAEKKRVYI 60 VLGKPIEV+SADH NKADIAAT+AREWAD+RGLDMLIGGVNSATAL+ NKVMAEKKRVYI Sbjct: 68 VLGKPIEVISADHQNKADIAATRAREWADERGLDMLIGGVNSATALSMNKVMAEKKRVYI 127 Query: 61 NIGAGSARLTNEE 73 NIGAG+ARLTNEE Sbjct: 128 NIGAGTARLTNEE 140 >gi|300314071|ref|YP_003778163.1| amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 402 Score = 253 bits (647), Expect = 1e-68, Method: Compositional matrix adjust. Identities = 147/309 (47%), Positives = 177/309 (57%), Gaps = 74/309 (23%) Query: 3 GKPIEVLSADHLNKADIAATKAREWADQRGLDMLIGGVNSATALATNKVMAEKKRVYINI 62 GK IE+L ADH NKAD+AA+KAREW DQ+G+DMLIGG NS TALA+ KV AEKK+VY+ I Sbjct: 68 GKKIELLVADHQNKADVAASKAREWFDQQGVDMLIGGTNSGTALASAKVAAEKKKVYMQI 127 Query: 63 GAGSARLTNEEXN--------------IGAGSARLTFLLQAQGSKAQILGLANAG----- 103 GAGSARLTNEE + G GSA + QG K+ A+ Sbjct: 128 GAGSARLTNEECSPYTVHYAYDTVALAKGTGSAVVK-----QGGKSWFFLTADYAFGHSL 182 Query: 104 -DDTVNAIKA------------------------------------------------AK 114 DT N +KA A Sbjct: 183 ESDTANVVKASGGTVVGAVKHPLGASDFSSFLLQAQSSKAQIIGLANAGGDFINSVKAAN 242 Query: 115 EFGVTKTMKLVGLLMVINDIHALGLNNAEGLMMTDSWYWDKDDASRQFANRFFLKMKKMP 174 EFG+TKT KL GLL+ IND+H+L L N EGL +TDSWYWD ++ +R FA R+F KKMP Sbjct: 243 EFGITKTAKLAGLLVFINDVHSLTLKNTEGLYLTDSWYWDANEETRAFAKRYFAVRKKMP 302 Query: 175 STHQAAAYSATTTYLKAVAAIKTDDSTKVMAELKKMKIDDFYNK-GYIRADGRNIHDMYL 233 S+ QAA YSA YLK V I TDDS KVMA L+K K +D Y K GY+RADGR +HDMYL Sbjct: 303 SSLQAADYSAALQYLKVVKEIGTDDSDKVMAALRKAKFNDMYQKNGYLRADGRVMHDMYL 362 Query: 234 YQVKSPXDA 242 YQVK+ ++ Sbjct: 363 YQVKAQSES 371 >gi|300309490|ref|YP_003773582.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 404 Score = 199 bits (506), Expect = 3e-52, Method: Compositional matrix adjust. Identities = 95/160 (59%), Positives = 121/160 (75%), Gaps = 1/160 (0%) Query: 84 TFLLQAQGSKAQILGLANAGDDTVNAIKAAKEFGVTKTMKLVGLLMVINDIHALGLNNAE 143 +FLLQAQ SKAQILGLA AG D +NAIKAA EFGV KTMKL GLL+ IND H+LGL+ + Sbjct: 214 SFLLQAQSSKAQILGLAVAGGDVINAIKAANEFGVNKTMKLAGLLIFINDTHSLGLSLTQ 273 Query: 144 GLMMTDSWYWDKDDASRQFANRFFLKMKKMPSTHQAAAYSATTTYLKAVAAIKTDDSTKV 203 G+ +TD WYWD +D +R +ANR+F+KMKKMPS QA SA YLKAV A+ + D+ KV Sbjct: 274 GMYLTDGWYWDLNDQTRAWANRYFMKMKKMPSMFQAGDASAVGQYLKAVKAVGSTDADKV 333 Query: 204 MAELKKMKIDDFY-NKGYIRADGRNIHDMYLYQVKSPXDA 242 M L+K +I+DF+ + G +R DGR +HDMYL +VK P ++ Sbjct: 334 MDYLRKNRINDFFTSNGVVRPDGRMVHDMYLMEVKKPSES 373 >gi|300312372|ref|YP_003776464.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 410 Score = 153 bits (386), Expect = 2e-38, Method: Compositional matrix adjust. Identities = 80/162 (49%), Positives = 109/162 (67%), Gaps = 3/162 (1%) Query: 84 TFLLQAQGSKAQILGLANAGDDTVNAIKAAKEFGVT-KTMKLVGLLMVINDIHALGLNNA 142 +FLLQAQ SKAQ++GLA+ G D VNAIK A+ F V K +LV LL+ ++D+HALGL A Sbjct: 218 SFLLQAQASKAQVIGLADGGTDVVNAIKQARSFAVGGKDQRLVALLVFLSDVHALGLEAA 277 Query: 143 EGLMMTDSWYWDKDDASRQFANRFFLKMKKM-PSTHQAAAYSATTTYLKAVAAIKTDDST 201 +GL+ TD +YWD D+ +R F+ RF + K + P+ QA YS+ +LKA AA KT D Sbjct: 278 QGLVYTDGFYWDYDEQTRAFSKRFESQFKNIKPTMVQAGVYSSVYQFLKARAAAKTSDWK 337 Query: 202 KVMAELKKMKI-DDFYNKGYIRADGRNIHDMYLYQVKSPXDA 242 V +++++ I D IR DGR IHDMYLYQVK+P ++ Sbjct: 338 VVAQKMREIPIADAVMRNASIRPDGRVIHDMYLYQVKTPAES 379 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.317 0.130 0.374 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 948,693 Number of extensions: 36294 Number of successful extensions: 154 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 149 Number of HSP's successfully gapped: 8 Length of query: 320 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 235 Effective length of database: 1,217,395 Effective search space: 286087825 Effective search space used: 286087825 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 130 (54.7 bits)