BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0005.0172.p (145 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312076|ref|YP_003776168.1| signal transduction histidine k... 66 1e-12 >gi|300312076|ref|YP_003776168.1| signal transduction histidine kinase [Herbaspirillum seropedicae SmR1] Length = 356 Score = 65.9 bits (159), Expect = 1e-12, Method: Compositional matrix adjust. Identities = 39/84 (46%), Positives = 52/84 (61%), Gaps = 1/84 (1%) Query: 9 PDPTADRIKEAERRRIAKDLHDELGSHLTALKMALAQLGGQLPRDN-DALLDQARHADGL 67 P TA I+E ER RIA+DLHD+LG L + MAL +L QL A L +A A L Sbjct: 143 PPATAIHIREQERERIARDLHDDLGGVLAGVGMALGRLQEQLAAQGLAAPLAEADEARQL 202 Query: 68 IDGALEAMHDIIDDLHPAVLDLGL 91 + A+ +MH IID+LHP +++ GL Sbjct: 203 LQQAMASMHSIIDELHPPIVEFGL 226 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.137 0.395 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 527,050 Number of extensions: 23034 Number of successful extensions: 88 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 87 Number of HSP's successfully gapped: 1 Length of query: 145 Length of database: 1,619,870 Length adjustment: 77 Effective length of query: 68 Effective length of database: 1,255,275 Effective search space: 85358700 Effective search space used: 85358700 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 126 (53.1 bits)