BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0005.0153.p (235 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312089|ref|YP_003776181.1| lipoprotein release ABC transpo... 303 8e-84 gi|300312904|ref|YP_003776996.1| diguanylate cyclase/phosphodies... 60 2e-10 >gi|300312089|ref|YP_003776181.1| lipoprotein release ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 421 Score = 303 bits (776), Expect = 8e-84, Method: Compositional matrix adjust. Identities = 147/167 (88%), Positives = 157/167 (94%) Query: 20 LSLIKNLPFEWLVGIRYTRAGKRSGRNSFISFISLISMAGIGLGVAALIVVLSVMNGFQK 79 +SLIKNLPFEWLVGIRYTRAGKRSGRNSFISFISLISMAGI LGVAALIVVLSVMNGFQK Sbjct: 1 MSLIKNLPFEWLVGIRYTRAGKRSGRNSFISFISLISMAGIALGVAALIVVLSVMNGFQK 60 Query: 80 EVRDRMLSVLSHIEVFDASGAMPDWQAVAKQTFQNKEVIGAAPYVAAQAMMTRDDNVRGV 139 EVRDRMLSVL+HIEVFDASG+M DWQAVAKQTFQN EV+GAAPYVAAQAMMTRDD+VRGV Sbjct: 61 EVRDRMLSVLAHIEVFDASGSMKDWQAVAKQTFQNPEVLGAAPYVAAQAMMTRDDSVRGV 120 Query: 140 VIRGVLPAEEPKVSDVAGQVRQGKFSDLKPGEFNIVLGGELARSLHL 186 ++RGVLP EEPKVSDVA QV+QG F LKPGEFNIV+GGELAR L + Sbjct: 121 LVRGVLPQEEPKVSDVAKQVKQGSFDSLKPGEFNIVIGGELARGLRV 167 >gi|300312904|ref|YP_003776996.1| diguanylate cyclase/phosphodiesterase with PAS/PAC sensor domains protein [Herbaspirillum seropedicae SmR1] Length = 701 Score = 59.7 bits (143), Expect = 2e-10, Method: Compositional matrix adjust. Identities = 26/42 (61%), Positives = 32/42 (76%) Query: 180 LARSLHLEVLAEGVEDLTQYDIMKENGCDKVQGYYFSKPLAV 221 LA L+L V+AEGVED Q+ +KEN CD+VQGYYF KP+ V Sbjct: 640 LAHILNLRVIAEGVEDEEQHAFLKENACDEVQGYYFGKPMEV 681 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.136 0.388 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 733,824 Number of extensions: 30321 Number of successful extensions: 99 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 99 Number of HSP's successfully gapped: 2 Length of query: 235 Length of database: 1,619,870 Length adjustment: 82 Effective length of query: 153 Effective length of database: 1,231,600 Effective search space: 188434800 Effective search space used: 188434800 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 129 (54.3 bits)