BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0005.0147.p (416 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300314033|ref|YP_003778125.1| acyl-CoA dehydrogenase [Herbasp... 162 6e-41 gi|300314032|ref|YP_003778124.1| acyl-CoA dehydrogenase [Herbasp... 155 8e-39 gi|300310289|ref|YP_003774381.1| dibenzothiophene desulfurizatio... 145 7e-36 gi|300310678|ref|YP_003774770.1| phenol/naphthocyclinone hydroxy... 68 2e-12 >gi|300314033|ref|YP_003778125.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 411 Score = 162 bits (409), Expect = 6e-41, Method: Compositional matrix adjust. Identities = 129/378 (34%), Positives = 192/378 (50%), Gaps = 20/378 (5%) Query: 45 ELARTAAERDKAGGHAAAQKELLRQHGLLLLAVPKEYGGIGASWSETFAIVRRIARVDRX 104 E A +AA+RD+ Q E Q GL + VP+ +GGIGASW ++R I+ D Sbjct: 36 EFATSAAQRDRERILPFEQIERFSQSGLWAITVPRSHGGIGASWQTVAEVIRLISAADPS 95 Query: 105 AHLLA---FQTLQLSGVISYGSDRQRQLYLGETVALNHWWG-----NSANPADPRLVAT- 155 + F L L + S Q++L+ E + N N D R AT Sbjct: 96 IGQIPQNHFGNLNL--IARLASQEQQRLFFAEVLDGARLGNAGPERNGKNVLDVRTRATL 153 Query: 156 ---EHDGGLVLQGIKGFCSGTRGSHRLLTTAIHQPSGKLLIAVLPTAREGISIHDDWDPI 212 DG +L G + + +G +H + + AI G+ L+ G+ + DDW Sbjct: 154 DRAAGDGSYLLNGTRFYSTGALYAHWIPSRAIDD-DGQPLVVYTRRDAPGVRVIDDWSSF 212 Query: 213 GQRQTDSTSVSYEQVRLEWSEVLHRPDGVSTPFLTMRGVVAQLILVNLYLGMAEGAFEQA 272 GQR T S +V ++ VR+ ++VL D + P G V+QLI + G+A A + Sbjct: 213 GQRTTASGTVVFDNVRVPATQVLALKDALQQP--NNIGPVSQLIQAAIDAGIAHAAVQDT 270 Query: 273 RDYTLNQGRPWFASGVQRAADDPYVIHRYAEMHLKLRAARALTDEAGAEVDRAWGIGREL 332 + + RP+ SG++RA+DDPY++ + L+L AA AL ++A A +D + +L Sbjct: 271 IAFVTTRSRPYVDSGLERASDDPYILRDIGNLSLRLHAADALLEDAAALLD---SLPAQL 327 Query: 333 TGEQRGRAALAVAEAKILAHRAALAVGEDVFDVCGARATKAELGLDRFWRNARTHTLHDP 392 + EQ +A+ AVAEAK+L+ AL E +F++ G+ AT A LDR WRNAR HTLHDP Sbjct: 328 SFEQMAQASAAVAEAKVLSTELALLASEKLFELSGSAATLASHNLDRHWRNARVHTLHDP 387 Query: 393 IDYKLRDVGRYALEHLLP 410 + +K VG YAL P Sbjct: 388 VRWKYFAVGNYALNGTPP 405 >gi|300314032|ref|YP_003778124.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 421 Score = 155 bits (391), Expect = 8e-39, Method: Compositional matrix adjust. Identities = 123/388 (31%), Positives = 190/388 (48%), Gaps = 26/388 (6%) Query: 37 AALPAIEQELARTAAERDKAGGHAAAQKELLRQHGLLLLAVPKEYGGIGASWSETFAIVR 96 AA + ++ +R AA RD+ + E + GL + VPKEYGG G S+ ++ Sbjct: 40 AAARKLAEDFSREAALRDRERKLPWDELERYVRSGLWGITVPKEYGGAGVSYGTLAEVIA 99 Query: 97 RIARVDRX------AHLLAFQTLQLSGVISYGSDRQRQLYLGETVALNHWWGN------- 143 I+ D H A + ++++G S+ Q++ + E V +GN Sbjct: 100 TISAADGSIGQIPQNHFYAVELIRVNG-----SESQKRFFF-ERVLQGDRFGNALAELGT 153 Query: 144 -SANPADPRLVATEHDGGLVLQGIKGFCSGTRGSHRLLTTAIHQPSGKLLIAVLPTAREG 202 +++ RL H G + G K + +G + + T+ I G +A++P G Sbjct: 154 KTSHDRKTRLSRNAHGPGYRIDGRKFYATGALYADWVPTSVIDD-DGVQQLAIVPRHAPG 212 Query: 203 ISIHDDWDPIGQRQTDSTSVSYEQVRLEWSEVLHRPDGVSTPFLTMRGVVAQLILVNLYL 262 ++I DDW GQR T S S +E V + V+ S P T G +AQ+ + L Sbjct: 213 LTIIDDWSGFGQRTTGSGSAVFENVHVPADAVIGFATAFSRP--TQIGPMAQIQHAAIDL 270 Query: 263 GMAEGAFEQARDYTLNQGRPWFASGVQRAADDPYVIHRYAEMHLKLRAARALTDEAGAEV 322 G+A A+ + + R W SGV+RA DDP I ++ ++L AA AL AG +V Sbjct: 271 GIARAAYADLLTFVRTRSRAWVDSGVERAVDDPLTIREVGDLTIQLHAAEALLRRAGRKV 330 Query: 323 DRAWGIGRELTGEQRGRAALAVAEAKILAHRAALAVGEDVFDVCGARATKAELGLDRFWR 382 D A E + A++AVAEA++L +LA G +F++ G+++T AE LDR WR Sbjct: 331 DAAAAAPDE---DSVAAASIAVAEARVLTTEVSLAAGSKLFELAGSQSTLAEHNLDRHWR 387 Query: 383 NARTHTLHDPIDYKLRDVGRYALEHLLP 410 NARTHTLHDP+ +K +G Y L P Sbjct: 388 NARTHTLHDPVRWKYHAIGNYVLNGKKP 415 >gi|300310289|ref|YP_003774381.1| dibenzothiophene desulfurization flavin reductase [Herbaspirillum seropedicae SmR1] Length = 419 Score = 145 bits (365), Expect = 7e-36, Method: Compositional matrix adjust. Identities = 104/376 (27%), Positives = 174/376 (46%), Gaps = 12/376 (3%) Query: 41 AIEQELARTAAERDKAGGHAAAQKELLRQHGLLLLAVPKEYGGIGASWSETFAIVRRIAR 100 A+ LA+ A+ERD+ + E Q GL + VP+ +GG S++ ++ I+ Sbjct: 44 ALAPRLAQGASERDQLRRWPVEEIEAYSQSGLWAINVPRAFGGAEVSYATLAEVIAIISA 103 Query: 101 VDRXAHLLAFQTLQLSGVISYGSDRQRQLYLGETVALNHWWGNSANPADPRLVATEH--- 157 D + L + SD ++Q WGN+ + + + A Sbjct: 104 ADPSLGQITQNHLGFVAAVRTVSDPEQQRRFFADFLRGVRWGNAFSESGSKRAADFETRF 163 Query: 158 -DGG--LVLQGIKGFCSGTRGSHRLLTTAIHQPSGKLLIAVLPTAREGISIHDDWDPIGQ 214 D G +++ G K + +G +H + A + + V G+++ DDW GQ Sbjct: 164 VDAGDHVIVTGKKFYSTGALLAHYVPIVA-NDEENRTWYVVAERNAPGLTVIDDWSAFGQ 222 Query: 215 RQTDSTSVSYEQVRLEWSEVLHRPDGVSTPFLTMRGVVAQLILVNLYLGMAEGAFEQARD 274 R T S +V +QV++ + ++ G P + G + Q+I + +G+A A +D Sbjct: 223 RTTLSGTVIIDQVKVPKTHLIPAYKGYLEP--SADGAIFQIIQAAVDVGIARAAIADTQD 280 Query: 275 YTLNQGRPWFASGVQRAADDPYVIHRYAEMHLKLRAARALTDEAGAEVDRAWGIGRELTG 334 + + RPW S A +DPY I ++ ++L A+ AL + AG +D A Sbjct: 281 FVRTKSRPWIDSQRDHAWEDPYSIQAIGDLQIRLHASEALLERAGRAIDAAVAAP---DA 337 Query: 335 EQRGRAALAVAEAKILAHRAALAVGEDVFDVCGARATKAELGLDRFWRNARTHTLHDPID 394 + A + AEAK+L+ A+ +F++ G R+T + LDR WRNARTHTLHDP+ Sbjct: 338 QSVAHAQILTAEAKVLSTEIAIHATNKLFELAGTRSTLGQYNLDRHWRNARTHTLHDPVR 397 Query: 395 YKLRDVGRYALEHLLP 410 +K VG Y L + P Sbjct: 398 WKYAIVGNYYLNGVKP 413 >gi|300310678|ref|YP_003774770.1| phenol/naphthocyclinone hydroxylase [Herbaspirillum seropedicae SmR1] Length = 386 Score = 67.8 bits (164), Expect = 2e-12, Method: Compositional matrix adjust. Identities = 97/369 (26%), Positives = 155/369 (42%), Gaps = 30/369 (8%) Query: 37 AALPAIEQE-----LARTAAERDKAGGHAAAQKEL---LRQHGLLLLAVPKEYGGIGASW 88 AAL A E++ LA A RD+ K+ L++ G+ A P+ +GG G S Sbjct: 8 AALAAREEDALAPLLAEIRARRDEFAAQRFVPKDFIAQLKKAGVYRAATPQCFGGSGMSP 67 Query: 89 SETFAIVRRIARVDRXAHLLAFQTLQLSGVISYGSDRQRQLYLGETVALNHWWGNSANPA 148 ++ RI+ D +A + + + Q +LY + + P Sbjct: 68 MGFLQLIERISAADASVGWVASFGSASVYLAALPRETQAKLY---AKGPDLTFAGGLFPI 124 Query: 149 DPRLVATEHDGGLVLQGIKGFCSGTRGSHRL---LTTAIH-QPSGKLLIAVLPTAREGIS 204 P AT+ +GG ++ G F SG++G+ L + A+ Q K AVLP E + Sbjct: 125 QP---ATQVEGGWMVDGTWKFASGSKGADVLGVGIGAAVPGQAGSKPRTAVLPP--EQVE 179 Query: 205 IHDDWDPIGQRQTDS--TSVSYEQVRLEWSEVLHRPDGVSTPFLTMRGVV-AQLILVNLY 261 I D+WD +G T S VS + V EW+ + + P + A +L + Sbjct: 180 IIDNWDVMGLLGTGSHDLKVSQQFVPEEWTFIRGGEPTIDEPLYRYPTIAYAAQVLAVVN 239 Query: 262 LGMAEGAFEQARDYTLNQGRPWFASGVQRAADDPYVIHRYAEMHLKLRAARALTDEAGAE 321 LG+A A ++ ++ GR +G + AD YV A+ KLR+ARA +A E Sbjct: 240 LGVARAAIDEINQ--MSGGRVAI-TGAPKLADRSYVRIEVAKAEAKLRSARAFLYDATGE 296 Query: 322 VDRAWGIGRELTGEQRGRAALAVAEAKILAHRAALAVGEDVFDVCGARATKAELGLDRFW 381 V + G +T EQ L E + + V + +F + G A + L R+ Sbjct: 297 VWNSILAGNPVTPEQVSLLRLTAVEIAKVGNE----VTQAMFGLAGTTAIYSHHPLQRYL 352 Query: 382 RNARTHTLH 390 R+A H Sbjct: 353 RDAAVVKQH 361 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.134 0.408 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,324,793 Number of extensions: 54292 Number of successful extensions: 174 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 167 Number of HSP's successfully gapped: 5 Length of query: 416 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 328 Effective length of database: 1,203,190 Effective search space: 394646320 Effective search space used: 394646320 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 132 (55.5 bits)