BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0005.0125.p (174 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311212|ref|YP_003775304.1| phosphoglycolate phosphatase [H... 250 5e-68 >gi|300311212|ref|YP_003775304.1| phosphoglycolate phosphatase [Herbaspirillum seropedicae SmR1] Length = 219 Score = 250 bits (638), Expect = 5e-68, Method: Compositional matrix adjust. Identities = 121/129 (93%), Positives = 125/129 (96%) Query: 1 MARKQFDLIVFDWDGTLMDSTSTIVRCIQAAARDLGLPIPDKSAASYVIGLGLQDAMQAA 60 MARKQFDLIVFDWDGTLMDSTSTIVRCIQAAARDLGLPIPDKSAASYVIGLGLQDAMQAA Sbjct: 1 MARKQFDLIVFDWDGTLMDSTSTIVRCIQAAARDLGLPIPDKSAASYVIGLGLQDAMQAA 60 Query: 61 IPDVDPKYYPRIVERYRHHYLGQDKDLSLFDGVREMLDDLGQQGYFLAVATGKSRVGLNR 120 IPDVDPKYYPRIVERYRHHYLGQDKDL+LF+GV EML DL QQGYFLAVATGKSRVGLNR Sbjct: 61 IPDVDPKYYPRIVERYRHHYLGQDKDLTLFEGVPEMLADLSQQGYFLAVATGKSRVGLNR 120 Query: 121 ALNSTKLLS 129 A+N+T LLS Sbjct: 121 AMNTTGLLS 129 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.138 0.411 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 584,482 Number of extensions: 24386 Number of successful extensions: 76 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 76 Number of HSP's successfully gapped: 1 Length of query: 174 Length of database: 1,619,870 Length adjustment: 79 Effective length of query: 95 Effective length of database: 1,245,805 Effective search space: 118351475 Effective search space used: 118351475 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 127 (53.5 bits)