BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0005.0123.p (823 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311210|ref|YP_003775302.1| ribonuclease E protein [Herbasp... 1204 0.0 gi|300312859|ref|YP_003776951.1| cytoplasmic axial filament ribo... 262 6e-71 >gi|300311210|ref|YP_003775302.1| ribonuclease E protein [Herbaspirillum seropedicae SmR1] Length = 1062 Score = 1204 bits (3116), Expect = 0.0, Method: Compositional matrix adjust. Identities = 632/755 (83%), Positives = 655/755 (86%), Gaps = 48/755 (6%) Query: 1 MLFNATQQEELRVAIVDGQKLIDIDIEATGREQRKSNIYKGVITRIEPSLEACFVNYGEE 60 MLFNATQQEELRVAIVDGQKLIDIDIE TGREQRKSNIYKGVITRIEPSLEACFVNYGEE Sbjct: 4 MLFNATQQEELRVAIVDGQKLIDIDIETTGREQRKSNIYKGVITRIEPSLEACFVNYGEE 63 Query: 61 RHGFLPFKEVARTYFKEGIDVRNASIKEALREGQEIIVQVEKEERGNKGAALTSFISLAG 120 RHGFLPFKEVAR+YFKEGIDVRNASIKEALREGQEIIVQVEKEERGNKGAALTSFISLAG Sbjct: 64 RHGFLPFKEVARSYFKEGIDVRNASIKEALREGQEIIVQVEKEERGNKGAALTSFISLAG 123 Query: 121 RYLVLMPNNPRGGGVSRRVEGEDRQELRETMDKLDLPNGMSVIARTAGIGRNVDELQWDL 180 RYLVLMPNNPRGGGVSRRVEGEDRQELRETMDKLDLPNGMSVIARTAGIGRNVDELQWDL Sbjct: 124 RYLVLMPNNPRGGGVSRRVEGEDRQELRETMDKLDLPNGMSVIARTAGIGRNVDELQWDL 183 Query: 181 NYLMQLWRAIEGAGQSGSGAFLIYQESSLVIRAIRDYFQPDIGEILIDTDDIHEQAQQFM 240 NYLMQLWRAIEGAGQS SGAFLIYQESSLVIRAIRDYFQPDIGEILIDTDDIH+QAQQFM Sbjct: 184 NYLMQLWRAIEGAGQSASGAFLIYQESSLVIRAIRDYFQPDIGEILIDTDDIHDQAQQFM 243 Query: 241 AHVMPDMVHRVKRYHDDVPLFSRFQIEHQIETAYSRTVPLPSGGAIVIDHTEALVSVDVN 300 AHVMPDMVHRVKRY DDVPLFSRFQIEHQIETAYSRTVPLPSGGAIVIDHTEALVSVDVN Sbjct: 244 AHVMPDMVHRVKRYRDDVPLFSRFQIEHQIETAYSRTVPLPSGGAIVIDHTEALVSVDVN 303 Query: 301 SARATRGSDIETTAFYTNLEAADEVARQLRLRDLGGLIVIDFIDMENAKNQREVESRLKD 360 SARATRGSDIETTAF TNLEAADEVARQLRLRDLGGLIVIDFIDMEN+KNQREVE RLKD Sbjct: 304 SARATRGSDIETTAFNTNLEAADEVARQLRLRDLGGLIVIDFIDMENSKNQREVEGRLKD 363 Query: 361 ALHHDRARVQMGKISRFGLMELSRQRLRPSLSEGSHVTCPRCNGTGHIRDTESSALQVLR 420 ALHHDRARVQMGKISRFGLMELSRQRLRPSLSEGSHVTCPRCNGTGHIRDTESSALQVLR Sbjct: 364 ALHHDRARVQMGKISRFGLMELSRQRLRPSLSEGSHVTCPRCNGTGHIRDTESSALQVLR 423 Query: 421 IIQEEAMKENSAAIHVQAPVDVAAFLLNEKRGEILKIETRHRVTVIMIPNKHLETPHYKL 480 IIQEEAMKENSAAIHVQAPVDVAAFLLNEKRGEILKIETRHRVTVIMIPNKHLETPHYK+ Sbjct: 424 IIQEEAMKENSAAIHVQAPVDVAAFLLNEKRGEILKIETRHRVTVIMIPNKHLETPHYKM 483 Query: 481 ERIKHDDPRLDDAQASYSMAEQAETDIGYSKRQKEDIKPRQEAVVKGITPDQPAPIVERK 540 ERIKHDDPRLDDAQASY+MAEQA+TDIGYSKRQKED++PRQEAVVKGITPDQPAPIVERK Sbjct: 484 ERIKHDDPRLDDAQASYTMAEQADTDIGYSKRQKEDVRPRQEAVVKGITPDQPAPIVERK 543 Query: 541 ---VATPVEPPPTLAPESGFFNKIWSFFRAKPVEATPAVAPVETKSPQKRD--------- 588 PPPTLAPESG KI+SFFR KPV A APVE K+P KR+ Sbjct: 544 APEPVAAPVPPPTLAPESGLLGKIFSFFRKKPVAPVAAPAPVEAKAPAKREGERNGRGGD 603 Query: 589 -------------HADRNSR-NRNGRNRNGRNRNEREERDGAAPEKTARDESGKQEYEGK 634 A+R R RNG RNGRN AA E A D + EGK Sbjct: 604 RNGRNRNRNERGDRAERGERAERNGEQRNGRN--------PAAAELKAGDAA-----EGK 650 Query: 635 PARPPRPPREPKEAREPREPRE-GAEGRNDREPREPREPRQPREGREPREPKEAREPREG 693 R PRPPREPKE REPREPRE A+ R+ +E +E R +PRE REPRE +E REPREG Sbjct: 651 QVRAPRPPREPKEGREPREPRELQAQARDGQEVKETRR-NEPREAREPREGREGREPREG 709 Query: 694 SEAREPRQPRTPRPPREERKDLPKAEDGLPLEAAL 728 R+PR PR PR ERK+ K+E+GLPLEAAL Sbjct: 710 ------REPRAPRAPRGERKEA-KSEEGLPLEAAL 737 >gi|300312859|ref|YP_003776951.1| cytoplasmic axial filament ribonuclease G protein [Herbaspirillum seropedicae SmR1] Length = 487 Score = 262 bits (670), Expect = 6e-71, Method: Compositional matrix adjust. Identities = 157/453 (34%), Positives = 250/453 (55%), Gaps = 6/453 (1%) Query: 1 MLFNATQQEELRVAIVDGQKLIDIDIEATGREQRKSNIYKGVITRIEPSLEACFVNYGEE 60 +L N T QE RVA++ + ++ IE T NIY G + R+ P +++ F++ G E Sbjct: 5 ILINITPQET-RVALILQGAVQELHIERTLSRGLAGNIYLGKVVRVLPGMQSAFIDIGLE 63 Query: 61 RHGFLPFKEVARTYFKEGIDVRNASIKEALREGQEIIVQVEKEERGNKGAALTSFISLAG 120 R FL ++ +G + I++ L +GQ I VQV K+ G KGA L++ IS+AG Sbjct: 64 RAAFLHVADIWEARSHDGQNTPAIPIEKLLHDGQSITVQVIKDPIGTKGARLSTQISIAG 123 Query: 121 RYLVLMPNNPRGGGVSRRVEGEDRQELRETMDKLDLP---NGMSVIARTAGIGRNVDELQ 177 R LV +P + + G+S+R+E E +EL + + LP G +I A + D LQ Sbjct: 124 RMLVYLPQD-KHIGISQRIENEAERELLRSKVQALLPPEEKGGFIIRTMAEDASDAD-LQ 181 Query: 178 WDLNYLMQLWRAIEGAGQSGSGAFLIYQESSLVIRAIRDYFQPDIGEILIDTDDIHEQAQ 237 D+ YL + W I G++ L++Q+ SL R +RD+ I +D+ + Q Sbjct: 182 MDVEYLRKTWATIVKQGKTNPAPTLLHQDLSLAQRVLRDFVGDHTATIQVDSRENFHMLQ 241 Query: 238 QFMAHVMPDMVHRVKRYHDDVPLFSRFQIEHQIETAYSRTVPLPSGGAIVIDHTEALVSV 297 +F A P ++ ++ Y + PLF + +E +IE A R V L SGG +++D TEA+ ++ Sbjct: 242 EFGAIYTPSVLTKLTHYRGERPLFDLYGVEEEIERALGRRVDLKSGGYLIVDQTEAMTTI 301 Query: 298 DVNSARATRGSDIETTAFYTNLEAADEVARQLRLRDLGGLIVIDFIDMENAKNQREVESR 357 DVN+ G + + T F TNLEAA +ARQLRLR+LGG+I++DFIDME+ ++++ V + Sbjct: 302 DVNTGSFVNGRNFDDTIFKTNLEAAHAIARQLRLRNLGGIIILDFIDMESTEHRQAVLAE 361 Query: 358 LKDALHHDRARVQMGKISRFGLMELSRQRLRPSLSEGSHVTCPRCNGTGHIRDTESSALQ 417 L AL DR ++ + S GL+E++R+R R SL+ TCP C G G ++ + + Sbjct: 362 LGKALQRDRTKISVSGFSTLGLVEMTRKRTRESLAHILCETCPACKGRGQVKTPRTVCYE 421 Query: 418 VLRIIQEEAMKENSAAIHVQAPVDVAAFLLNEK 450 +LR + EA + N + A V L E+ Sbjct: 422 ILREVMREAKQFNPREFRILASQVVVDMFLEEE 454 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.316 0.134 0.380 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,881,590 Number of extensions: 74675 Number of successful extensions: 223 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 220 Number of HSP's successfully gapped: 2 Length of query: 823 Length of database: 1,619,870 Length adjustment: 93 Effective length of query: 730 Effective length of database: 1,179,515 Effective search space: 861045950 Effective search space used: 861045950 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 135 (56.6 bits)