BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0005.0120.p (260 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311209|ref|YP_003775301.1| molybdenum cofactor biosynthesi... 379 e-106 gi|300312938|ref|YP_003777030.1| molybdenum cofactor biosynthesi... 140 7e-35 >gi|300311209|ref|YP_003775301.1| molybdenum cofactor biosynthesis protein A [Herbaspirillum seropedicae SmR1] Length = 393 Score = 379 bits (972), Expect = e-106, Method: Compositional matrix adjust. Identities = 193/260 (74%), Positives = 218/260 (83%), Gaps = 6/260 (2%) Query: 1 MLHELRTPDGRELDLTLTTNGTLLAQKAQALKDAGLKRVTVSLDALDDANFKRMNDVDVP 60 ML LRTP+ +DLTLTTNGTLL QKAQALKDAGLKRVTVSLD+LDD F+RMNDVD P Sbjct: 140 MLAALRTPEDEPVDLTLTTNGTLLKQKAQALKDAGLKRVTVSLDSLDDEGFRRMNDVDFP 199 Query: 61 VSQILHGLDVAHKVGLGPIKINMVVKKGVNDHEILTMARHFKDTPFTLRFIEYMDVGSSN 120 V+++L G+D AH GLGP+KINMVVKKGVND +IL MAR+FK TP LRFIEYMDVGSSN Sbjct: 200 VARVLEGIDAAHAAGLGPVKINMVVKKGVNDDQILPMARYFKGTPAILRFIEYMDVGSSN 259 Query: 121 GWRMDEVVPSSEVIRRIHAELPLEAIDANYTGETAQRWRYQDGSGEIGVISSVTQAFCQD 180 GWRMDEV+PS+EV+R I E+PLE D NYTGETA+RWRY DGSGEIGVISSVTQAFCQD Sbjct: 260 GWRMDEVLPSAEVVRLIATEMPLEVADPNYTGETAERWRYVDGSGEIGVISSVTQAFCQD 319 Query: 181 CSRARLSTEGKIYTCLFAGEGHDLRGLLRGERSDAEISSAIAAIWGARGDRYSELRSEQK 240 CSRARLSTEG++YTCLFA EGHDLR ++R SDAEI+ AIAA+WG RGDRYSELRS + Sbjct: 320 CSRARLSTEGRVYTCLFASEGHDLRSIVRSGGSDAEIAGAIAALWGQRGDRYSELRSALR 379 Query: 241 GSSTTSMPQVRKVEMSYIGG 260 + +RKVEMSYIGG Sbjct: 380 EAP------LRKVEMSYIGG 393 >gi|300312938|ref|YP_003777030.1| molybdenum cofactor biosynthesis enzyme A protein [Herbaspirillum seropedicae SmR1] Length = 333 Score = 140 bits (354), Expect = 7e-35, Method: Compositional matrix adjust. Identities = 83/227 (36%), Positives = 124/227 (54%), Gaps = 3/227 (1%) Query: 14 DLTLTTNGTLLAQKAQALKDAGLKRVTVSLDALDDANFKRMNDVDVPVSQILHGLDVAHK 73 +L+L+TN T L + A +L+ AG++R+ VSLD+LD A + D ++ ++ GL+ A Sbjct: 100 ELSLSTNATQLHKHAASLRAAGVQRINVSLDSLDRACSASITGRDS-LASVMSGLEAAKA 158 Query: 74 VGLGPIKINMVVKKGVNDHEILTMARHFKDTPFTLRFIEYMDVGSSNGWRMDEVVPSSEV 133 G PIKINMV+ +GVN+ E+ MA+ + F LR IE M +G++ R + + Sbjct: 159 AGFAPIKINMVLLRGVNEQEVPQMAQFCFEHGFILRLIETMPMGATG--RSSPALELGPL 216 Query: 134 IRRIHAELPLEAIDANYTGETAQRWRYQDGSGEIGVISSVTQAFCQDCSRARLSTEGKIY 193 + A L + A G A+ W DG IG IS ++Q FC C+R RLS +G +Y Sbjct: 217 REALVARFGLRPLAAELGGGPARYWSTPDGRSTIGFISPISQHFCATCNRVRLSVDGTLY 276 Query: 194 TCLFAGEGHDLRGLLRGERSDAEISSAIAAIWGARGDRYSELRSEQK 240 CL E +LR LLR DA + SAI A + +R+ + QK Sbjct: 277 LCLGQEERFELRPLLRAGIDDAGLESAIRAAIELKPERHEFNEAPQK 323 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.317 0.134 0.387 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 686,305 Number of extensions: 25084 Number of successful extensions: 81 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 78 Number of HSP's successfully gapped: 2 Length of query: 260 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 177 Effective length of database: 1,226,865 Effective search space: 217155105 Effective search space used: 217155105 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 129 (54.3 bits)