BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0005.0117.p (323 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300314046|ref|YP_003778138.1| NAD-dependent aldehyde dehydrog... 226 2e-60 gi|300313241|ref|YP_003777333.1| NAD-dependent aldehyde dehydrog... 111 6e-26 gi|300310458|ref|YP_003774550.1| NADP-dependent succinate-semial... 101 8e-23 gi|300311221|ref|YP_003775313.1| NAD+ dependent acetaldehyde deh... 99 6e-22 gi|300313866|ref|YP_003777958.1| benzaldehyde dehydrogenase [Her... 97 2e-21 >gi|300314046|ref|YP_003778138.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 472 Score = 226 bits (576), Expect = 2e-60, Method: Compositional matrix adjust. Identities = 120/232 (51%), Positives = 156/232 (67%) Query: 25 RQDRLQRLRQLLLRHRDDIAAAISADFGNRSQHETQFLEVFTSLQAIDHALAHGKHWMQP 84 R DRL RL+ L+ H + AAI ADFG RS HET+ E+F + HA +H K WM+P Sbjct: 31 RMDRLARLQALMEEHETALIAAIDADFGGRSPHETRMAELFVLRAGLRHAQSHLKRWMRP 90 Query: 85 QTRPTAIWFKPAGNRLLPQPLGVVGIVTPWNYPLLLTIGPLACALAAGNLAMIKLSENTP 144 Q TA+ F P NRLL QPLGVVG+++PWNYPL L +GP ALAAGN ++K SE TP Sbjct: 91 QRVATALHFLPGSNRLLAQPLGVVGVISPWNYPLQLALGPALAALAAGNRVLLKPSELTP 150 Query: 145 AFGELFDRLIRQSFSEDEIIVINGGVDLAREFCALAFDHLIFTGSTAVGREVMRIASERL 204 L L+ + ++ +E+ V+ G + + F AL FDHL FTGST VGR++ ++A+ L Sbjct: 151 RLSALLAELVARYYAPEEMAVVTGDAAIGQAFAALPFDHLFFTGSTQVGRQIAQLAAANL 210 Query: 205 TPVTLELGGKSPAIIAADADFKHAVARIMNGKLLNAGQTCIAPDYVLLPHGR 256 TPVTLELGGKSPAI+ ++ RI GKLLNAGQTC+APDY+L+P G+ Sbjct: 211 TPVTLELGGKSPAILDRSCRLHSSLQRIAFGKLLNAGQTCVAPDYLLVPRGQ 262 >gi|300313241|ref|YP_003777333.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 484 Score = 111 bits (278), Expect = 6e-26, Method: Compositional matrix adjust. Identities = 86/235 (36%), Positives = 123/235 (52%), Gaps = 10/235 (4%) Query: 24 ERQDRLQRLRQLLLRHRDDIAAAISADFGNRSQHETQFLEVFTSLQAIDH--ALAHGKHW 81 +R L +L L+ R D +A + G RS E + ++V AI H A A + Sbjct: 67 QRGQLLHKLAALVERDTDLLADMDARAIG-RSPMEPRMMDV---PNAISHLRAAAGWANQ 122 Query: 82 MQPQTRPTAIWF-KPAGNRLLPQPLGVVGIVTPWNYPLLLTIGPLACALAAGNLAMIKLS 140 M+ +T PTA + KP + + +P+GVVG + PWN PL++T +A LAAG +IK + Sbjct: 123 MEGRTIPTAGYMGKPTLSYTVREPIGVVGAILPWNAPLMITSWKVAALLAAGCTVVIKPA 182 Query: 141 ENTPAFGELFDRLIRQSFSEDEII-VING-GVDLAREFCALA-FDHLIFTGSTAVGREVM 197 E TP RL +++ D +I V+ G G + R C + FTGS GR + Sbjct: 183 EETPQSALHLARLAQEAGFPDGVINVVTGYGNSVGRALCEHPDVAKISFTGSPEAGRAIQ 242 Query: 198 RIASERLTPVTLELGGKSPAIIAADADFKHAVARIMNGKLLNAGQTCIAPDYVLL 252 RIA E+ VTLELGGKSP I+ DA F+ A+ G +N GQ C A +L+ Sbjct: 243 RIAGEQFKRVTLELGGKSPQIVFDDAPFEDALFGCTLGLFVNQGQVCAAGSRILV 297 >gi|300310458|ref|YP_003774550.1| NADP-dependent succinate-semialdehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 493 Score = 101 bits (251), Expect = 8e-23, Method: Compositional matrix adjust. Identities = 80/257 (31%), Positives = 125/257 (48%), Gaps = 27/257 (10%) Query: 20 TPWEERQDRLQRLRQLLLRHRDDIAAAISADFGN---RSQHETQFLEVFTSLQAIDHALA 76 TP ++R ++R LLL H+DD+ IS + G ++ E + + + A Sbjct: 70 TPAKQRAGIIKRWNDLLLAHQDDLGRLISREQGKPLAEAKGEVAYAASYVEWFGEEATRA 129 Query: 77 HGKHWMQPQTRPTAIWFKPAGNRL--LPQPLGVVGIVTPWNYPLLLTIGPLACALAAGNL 134 +G P T G R+ L +P+GVV +TPWN+P + +A ALAAG Sbjct: 130 NGDIIPAPVT----------GRRMMALKEPVGVVAAITPWNFPAAMIARKIAPALAAGCT 179 Query: 135 AMIKLSENTPAFGELFDRLIRQSFSEDEIIVINGGVDLAREFCALAFD---------HLI 185 + K +E+TP RL +++ +I I V +RE D + Sbjct: 180 VVCKPAEDTPLTSLALVRLAQEAGVPVGVINI---VTASRERTPEVVDVWLADGRVRKIS 236 Query: 186 FTGSTAVGREVMRIASERLTPVTLELGGKSPAIIAADADFKHAVARIMNGKLLNAGQTCI 245 FTGSTAVG+ + R +++ L ++LELGG +P I+ DAD A+ +M K N GQTC+ Sbjct: 237 FTGSTAVGKHLARHSADTLKKLSLELGGNAPFIVFDDADVDAAIDGVMAAKFRNGGQTCV 296 Query: 246 APDYVLLPHGRERDFVD 262 +P+ + + FVD Sbjct: 297 SPNRIYVQEKVYDAFVD 313 >gi|300311221|ref|YP_003775313.1| NAD+ dependent acetaldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 506 Score = 98.6 bits (244), Expect = 6e-22, Method: Compositional matrix adjust. Identities = 63/169 (37%), Positives = 89/169 (52%), Gaps = 7/169 (4%) Query: 103 QPLGVVGIVTPWNYPLLLTIGPLACALAAGNLAMIKLSENTPAFGELFDRLIRQSFSEDE 162 +PLGVVG + PWN+P+L+ + LA ALAAGN ++K +E TPA + LI Sbjct: 153 EPLGVVGQIIPWNFPILMAVWKLAPALAAGNCVVLKPAEQTPASIMVLIELIADLIPPGV 212 Query: 163 IIVING-GVDLAREFCALA-FDHLIFTGSTAVGREVMRIASERLTPVTLELGGKSPAIIA 220 + ++ G GV+ + + + FTG T GR +M+ AS+ L PVTLELGGKSP I Sbjct: 213 VNIVQGFGVEAGKPLASNKRIAKIAFTGETTTGRLIMQYASQNLIPVTLELGGKSPNIFF 272 Query: 221 ADA--DFKHAVARIMNG---KLLNAGQTCIAPDYVLLPHGRERDFVDAA 264 AD + + G LN G+ C P VL+ F++ A Sbjct: 273 ADVLDKDDDFFDKALEGFAMFALNQGEVCTCPSRVLVQESIYERFIERA 321 >gi|300313866|ref|YP_003777958.1| benzaldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 487 Score = 97.1 bits (240), Expect = 2e-21, Method: Compositional matrix adjust. Identities = 80/255 (31%), Positives = 123/255 (48%), Gaps = 19/255 (7%) Query: 21 PWEERQDRLQRLRQLLLRHRDDIAAAISADFGN---RSQHETQFLEVFTSLQAIDHALAH 77 P ER D +R L +H D++A ++ + G + +HE + E T + H A Sbjct: 68 PPRERADIFRRAAGLFHQHFDELARMVARETGGVLFKGEHEVR--EAIT----LCHLAAG 121 Query: 78 GKHWMQPQTRPTAIWFKPAGNRLLPQPLGVVGIVTPWNYPLLLTIGPLACALAAGNLAMI 137 Q Q P+ R PLGV+G+++P+N+PL+LT+ +A ALAAGN ++ Sbjct: 122 MPLQAQGQLLPSTPGRLSIARR---APLGVIGVISPFNFPLILTLRTVAPALAAGNAVVV 178 Query: 138 KLSENTPAFGE-LFDRLIRQSFSEDEII-VINGGVDLAREFC-ALAFDHLIFTGSTAVGR 194 K TP G + R+ Q+ ++ V+ GG + A + FTGS AVGR Sbjct: 179 KPDLRTPVSGGFMLARIFEQAGLPAGLLHVLPGGAETGEALVTAPGVPMIAFTGSPAVGR 238 Query: 195 EVMRIASERLTPVTLELGGKSPAIIAADADFKHAVARIMNGKLLNAGQTCIAPDYVL--- 251 + +A L ++LELGG + +I DAD A + G L+ GQ C+A + +L Sbjct: 239 RIGELAGRHLKKLSLELGGANALVILEDADLDVAASHAAWGAWLHQGQICMAANRILVHA 298 Query: 252 -LPHGRERDFVDAAR 265 L G + D AR Sbjct: 299 SLEEGLLQRLADKAR 313 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.323 0.135 0.416 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,034,874 Number of extensions: 44685 Number of successful extensions: 154 Number of sequences better than 1.0e-08: 18 Number of HSP's gapped: 135 Number of HSP's successfully gapped: 18 Length of query: 323 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 238 Effective length of database: 1,217,395 Effective search space: 289740010 Effective search space used: 289740010 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.5 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 131 (55.1 bits)