BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0005.0108.p (146 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311201|ref|YP_003775293.1| threonine efflux protein [Herba... 219 9e-59 >gi|300311201|ref|YP_003775293.1| threonine efflux protein [Herbaspirillum seropedicae SmR1] Length = 219 Score = 219 bits (557), Expect = 9e-59, Method: Compositional matrix adjust. Identities = 112/152 (73%), Positives = 125/152 (82%), Gaps = 8/152 (5%) Query: 1 VASEAAFNTIKILGAAYLIYLGWSQWRSPVHVDEAQAARPAAPA--------AWWQRTVT 52 VASE AFN IK+ GA YLIYLG+SQWR+ V +E A AA A WWQR +T Sbjct: 66 VASETAFNVIKVAGALYLIYLGFSQWRARVPSEEQGEALDAANASARAHARQTWWQRCMT 125 Query: 53 GFLTNATNPKGIVFMVAVLPQFINHERSLWLQLLLMAITMVAVDLVVMHGYAFAASRMQR 112 GFLTNATNPKGIVFMVAVLPQFI+H+R LWLQLL++A+TMVAVDLVVMHGYAFAASR+QR Sbjct: 126 GFLTNATNPKGIVFMVAVLPQFIDHDRPLWLQLLVLAVTMVAVDLVVMHGYAFAASRLQR 185 Query: 113 FFRDAKAVRMQNRFFGGVLMLVGAGLFFFKRS 144 FFR KA+R QNRFFGGVLMLVGAGLFFFKR+ Sbjct: 186 FFRSQKAIRAQNRFFGGVLMLVGAGLFFFKRT 217 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.330 0.137 0.434 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 387,626 Number of extensions: 11622 Number of successful extensions: 54 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 56 Number of HSP's successfully gapped: 1 Length of query: 146 Length of database: 1,619,870 Length adjustment: 77 Effective length of query: 69 Effective length of database: 1,255,275 Effective search space: 86613975 Effective search space used: 86613975 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.9 bits) S2: 126 (53.1 bits)