BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0005.0084.p (416 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312245|ref|YP_003776337.1| transmembrane efflux transmembr... 446 e-126 gi|300312509|ref|YP_003776601.1| arabinose efflux permease [Herb... 280 1e-76 gi|300313915|ref|YP_003778007.1| transmembrane arabinose efflux ... 130 2e-31 gi|300310405|ref|YP_003774497.1| arabinose efflux permease [Herb... 107 3e-24 gi|300312340|ref|YP_003776432.1| transporter transmembrane prote... 62 6e-11 >gi|300312245|ref|YP_003776337.1| transmembrane efflux transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 395 Score = 446 bits (1147), Expect = e-126, Method: Compositional matrix adjust. Identities = 239/291 (82%), Positives = 260/291 (89%) Query: 1 MPIALWALAISAFAIGTTEFVIMGLLPEVAANLGTTLSSAGLLVTGYALGVFVGAPLVTA 60 MPIALWAL ISAFAIGTTEFVIMGLLPEVAANLG +LSSAGLLVTGYALGVFVGAPL+TA Sbjct: 1 MPIALWALTISAFAIGTTEFVIMGLLPEVAANLGVSLSSAGLLVTGYALGVFVGAPLLTA 60 Query: 61 TTGKLPRKTLLVSLMVLFVIGNIGCAIAPDYTTLMISRIVASFSHGAFFGVGSVVATGLV 120 TTG+LPRKTLLV+LMV+FV+GN+ CAIAPDYTTLMI R+VASF+HGAFFGVGSVVAT LV Sbjct: 61 TTGRLPRKTLLVALMVVFVVGNLACAIAPDYTTLMIGRVVASFAHGAFFGVGSVVATSLV 120 Query: 121 PKEKQASAIALMFTGLTVANVLGVPFGTWLGQMYGWRSTFWAVSAIGVVALLATAYLVPR 180 PKEKQASAIALMFTGLTVANVLGVPFGTWLGQ YGWR+TFWAV+ IGV+AL ATA LVPR Sbjct: 121 PKEKQASAIALMFTGLTVANVLGVPFGTWLGQSYGWRATFWAVTVIGVIALAATAVLVPR 180 Query: 181 SDDRQDADFRSELRVLGRPQVLLGLLMTVLGFGGVFAAFTYIAPILTDITGFQPGAVSPI 240 S ++ DF E+RVL RPQV+LGLLMTVLGFGGVFAAFTYIAPILTD++GF AVSPI Sbjct: 181 SQGGEETDFGKEVRVLMRPQVMLGLLMTVLGFGGVFAAFTYIAPILTDLSGFPASAVSPI 240 Query: 241 LLLFGVGLVGGNLLGGKFADKYLMPTLVISLVLLAGVLVLFSFTSHYKLAA 291 LLLFG+GLV GNL GGK ADK LM TL+ SLVLLA VLVLF T K+AA Sbjct: 241 LLLFGLGLVIGNLAGGKLADKKLMATLLGSLVLLAAVLVLFGLTVDNKVAA 291 >gi|300312509|ref|YP_003776601.1| arabinose efflux permease [Herbaspirillum seropedicae SmR1] Length = 385 Score = 280 bits (717), Expect = 1e-76, Method: Compositional matrix adjust. Identities = 154/284 (54%), Positives = 197/284 (69%) Query: 5 LWALAISAFAIGTTEFVIMGLLPEVAANLGTTLSSAGLLVTGYALGVFVGAPLVTATTGK 64 L+ALA++AF IGTTEFVIMGLLP+VA +LG T +AG+LV+GYALGV GAP++ T K Sbjct: 5 LFALAVAAFGIGTTEFVIMGLLPDVARDLGVTAPAAGMLVSGYALGVAAGAPILAILTAK 64 Query: 65 LPRKTLLVSLMVLFVIGNIGCAIAPDYTTLMISRIVASFSHGAFFGVGSVVATGLVPKEK 124 PRK LV LM LF++GN+ CA+AP+Y LM++R+V +F H AFFG+GSVVA LV +K Sbjct: 65 WPRKQALVGLMCLFILGNVLCALAPNYVFLMVARVVTAFCHAAFFGIGSVVAAELVAPQK 124 Query: 125 QASAIALMFTGLTVANVLGVPFGTWLGQMYGWRSTFWAVSAIGVVALLATAYLVPRSDDR 184 +A A+ALMFTGLTVAN+LGVP GT G GWRSTF AV+AIGV+A + ++P+ Sbjct: 125 RAQAVALMFTGLTVANILGVPLGTAFGHAAGWRSTFGAVTAIGVLAAIMLWTMLPKKIPM 184 Query: 185 QDADFRSELRVLGRPQVLLGLLMTVLGFGGVFAAFTYIAPILTDITGFQPGAVSPILLLF 244 Q + E R + +V L LL + L +F+ TYIA IL D+TGF S +LLLF Sbjct: 185 QAGNIFQEFRSIIDTRVQLSLLTSTLASISMFSVITYIAYILLDVTGFTTQQESWVLLLF 244 Query: 245 GVGLVGGNLLGGKFADKYLMPTLVISLVLLAGVLVLFSFTSHYK 288 G G+ G L+GGK AD LMP + + +A VL LFS TSHYK Sbjct: 245 GAGITVGGLVGGKLADWKLMPVMCGVFLCIAVVLGLFSITSHYK 288 >gi|300313915|ref|YP_003778007.1| transmembrane arabinose efflux transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 403 Score = 130 bits (327), Expect = 2e-31, Method: Compositional matrix adjust. Identities = 95/292 (32%), Positives = 156/292 (53%), Gaps = 11/292 (3%) Query: 8 LAISAFAIGTTEFVIMGLLPEVAANLGTTLSSAGLLVTGYALGVFVGAPLVTATTGKLPR 67 LA+ FAIGT F+I LLP +AA+ G ++ LV + L + + +P+ T + + R Sbjct: 29 LALGTFAIGTEGFMIAPLLPRMAADFGMSVPMVASLVVIFTLVLALSSPISTVLSAAMNR 88 Query: 68 KTLLVSLMVLFVIGNIGCAIAPDYTTLMISRIVASFSHGAFFGVGSVVATGLVPKEKQAS 127 + +L++ M F N+ A + +T L+++R++ + + G + S +A +V ++ Sbjct: 89 RRVLIAAMTAFTAANLLAACSSSFTGLLVARLLMAVAAGLYTPNASALAGAMVAPGQRGR 148 Query: 128 AIALMFTGLTVANVLGVPFGTWLGQMYGWRSTFWAVSAIGVVALLATAYLVPRS--DDRQ 185 A+A++ G+T + LG+P G +G +GWR+TF AVSA+G VAL VPR Sbjct: 149 ALAIVSGGMTSPSALGLPLGAVVGHAFGWRATFLAVSAMGAVALAGILLGVPRQAGAHLH 208 Query: 186 DADFRSELRVLGRPQVLLGLLMTVLGFGGVFAAFTYIAPILTDITGFQPGAVSPILLLFG 245 A R + V+G+PQV L +T+L G +AA+ YIAP L+ + F +S + L+G Sbjct: 209 VAGLRERMAVIGQPQVQRLLAVTLLWSVGAYAAYPYIAPYLSHVLSFGESRISLTVSLWG 268 Query: 246 VGLVGGNLLGGKFADKYLMPTLV-ISLVLLAGVLVLFSFTSHYKLAASRKAP 296 G +GG D+Y +V +SL+LL S + LAA+ P Sbjct: 269 AAAAVGVSVGGTLNDRYGSARVVRVSLLLL--------MLSFWTLAAATALP 312 >gi|300310405|ref|YP_003774497.1| arabinose efflux permease [Herbaspirillum seropedicae SmR1] Length = 422 Score = 107 bits (266), Expect = 3e-24, Method: Compositional matrix adjust. Identities = 76/236 (32%), Positives = 128/236 (54%) Query: 7 ALAISAFAIGTTEFVIMGLLPEVAANLGTTLSSAGLLVTGYALGVFVGAPLVTATTGKLP 66 +LA+ F++ T EF+ LL +A++L + +AG VT A+ V AP + T + Sbjct: 41 SLALGVFSLVTAEFLPASLLTAIASDLHVSDGAAGQAVTATAIVGAVAAPSIPLLTRNID 100 Query: 67 RKTLLVSLMVLFVIGNIGCAIAPDYTTLMISRIVASFSHGAFFGVGSVVATGLVPKEKQA 126 RK +++ L +L ++ NI AP+ L+++R++ + G F+ + + +A LVP A Sbjct: 101 RKRVVLMLSLLLLVSNILAVTAPNLPVLLVARVLLGVALGGFWSMAAALAMRLVPDHLFA 160 Query: 127 SAIALMFTGLTVANVLGVPFGTWLGQMYGWRSTFWAVSAIGVVALLATAYLVPRSDDRQD 186 A++L+ TG++VA V P G ++G ++GWRS F A + +VA+ +PR + Sbjct: 161 RAMSLILTGVSVATVCAAPIGAYMGNLWGWRSAFVAAGVVSLVAIAIQWIAIPRLPPKDH 220 Query: 187 ADFRSELRVLGRPQVLLGLLMTVLGFGGVFAAFTYIAPILTDITGFQPGAVSPILL 242 + +LGR V + LL +L G FA FTYI P++ IT +S +LL Sbjct: 221 PSLKVLAELLGRKDVRVALLAVLLVVSGHFAGFTYIRPLMEQITHMSVTTISAVLL 276 >gi|300312340|ref|YP_003776432.1| transporter transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 421 Score = 62.4 bits (150), Expect = 6e-11, Method: Compositional matrix adjust. Identities = 62/260 (23%), Positives = 108/260 (41%), Gaps = 7/260 (2%) Query: 8 LAISAFAIGTTEFVIMGLLPE-VAANLGTTLSSAGLLVTGYALGVFVGAPLVTATTGKLP 66 LA FA+ +F++M L + ++ T GL+V+ YA L + Sbjct: 27 LAFLQFAV-ILDFMLMSPLGALIMPSMQITPQQFGLVVSAYAFSAGASGLLTAGFADRFD 85 Query: 67 RKTLLVSLMVLFVIGNIGCAIAPDYTTLMISRIVASFSHGAFFGVGSVVATGLVPKEKQA 126 RK LL+ FV+G + C +A + +L+++RIV G + +AT L + Sbjct: 86 RKRLLLFFYGGFVLGTVWCGLAQSFESLLLARIVTGLFGGVIGSITLAIATDLFAPHLRG 145 Query: 127 SAIALMFTGLTVANVLGVPFGTWLGQMYGWRSTFWAVSAIGVVALLATAYLVPRSD---- 182 + ++ T + VLG+P G +L + W F A++A+G+ L + D Sbjct: 146 RVMGVIQTSFAASQVLGIPIGLYLSNRWNWHVPFLAMAALGLAGGLVVLVRMQPVDGHLT 205 Query: 183 -DRQDADFRSELRVLGRPQVLLGLLMTVLGFGGVFAAFTYIAPILTDITGFQPGAVSPIL 241 ++ + + L P+ L T L G F + + L G + I Sbjct: 206 LKQEHSAWMHLYHTLAEPRYLTAFATTALLMTGGFMLMPFSSAYLVGNLGIDMAHLPTIY 265 Query: 242 LLFGVGLVGGNLLGGKFADK 261 L+ G+ + L G+ ADK Sbjct: 266 LVTGLCTIAFGPLIGRAADK 285 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.325 0.136 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,104,828 Number of extensions: 43116 Number of successful extensions: 320 Number of sequences better than 1.0e-08: 10 Number of HSP's gapped: 324 Number of HSP's successfully gapped: 10 Length of query: 416 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 328 Effective length of database: 1,203,190 Effective search space: 394646320 Effective search space used: 394646320 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 132 (55.5 bits)