BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0005.0083.p (389 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312245|ref|YP_003776337.1| transmembrane efflux transmembr... 573 e-164 gi|300312509|ref|YP_003776601.1| arabinose efflux permease [Herb... 344 9e-96 gi|300313915|ref|YP_003778007.1| transmembrane arabinose efflux ... 136 3e-33 gi|300310405|ref|YP_003774497.1| arabinose efflux permease [Herb... 110 2e-25 gi|300312340|ref|YP_003776432.1| transporter transmembrane prote... 63 3e-11 >gi|300312245|ref|YP_003776337.1| transmembrane efflux transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 395 Score = 573 bits (1476), Expect = e-164, Method: Compositional matrix adjust. Identities = 311/394 (78%), Positives = 340/394 (86%), Gaps = 5/394 (1%) Query: 1 MPIALWALAISAFAIGTTEFVIMGLLPEVAANLGTTLSSAGLLVTGYALGVFVGAPLVTA 60 MPIALWAL ISAFAIGTTEFVIMGLLPEVAANLG +LSSAGLLVTGYALGVFVGAPL+TA Sbjct: 1 MPIALWALTISAFAIGTTEFVIMGLLPEVAANLGVSLSSAGLLVTGYALGVFVGAPLLTA 60 Query: 61 TTGKLPRKTLLVSLMVLFVIGNIGCAIAPDYTTLMISRIVASFSHGAFFGVGSVVATGLV 120 TTG+LPRKTLLV+LMV+FV+GN+ CAIAPDYTTLMI R+VASF+HGAFFGVGSVVAT LV Sbjct: 61 TTGRLPRKTLLVALMVVFVVGNLACAIAPDYTTLMIGRVVASFAHGAFFGVGSVVATSLV 120 Query: 121 PKEKQASAIALMFTGLTVANVLGVPFGTWLGQMYGWRSTFWAVSAIGVVALLATAYLVPR 180 PKEKQASAIALMFTGLTVANVLGVPFGTWLGQ YGWR+TFWAV+ IGV+AL ATA LVPR Sbjct: 121 PKEKQASAIALMFTGLTVANVLGVPFGTWLGQSYGWRATFWAVTVIGVIALAATAVLVPR 180 Query: 181 SDDRQDADFRSELRVLGRPQVLLGLLMTVLGFGGVFAAFTYIAPILTDITGFQPGAVSPI 240 S ++ DF E+RVL RPQV+LGLLMTVLGFGGVFAAFTYIAPILTD++GF AVSPI Sbjct: 181 SQGGEETDFGKEVRVLMRPQVMLGLLMTVLGFGGVFAAFTYIAPILTDLSGFPASAVSPI 240 Query: 241 LLLFGVGLVGGNLLGGKFADKYLMPTLVISLVLLAGVLVLFSFTSHYKL-----VGLLGA 295 LLLFG+GLV GNL GGK ADK LM TL+ SLVLLA VLVLF T K+ VGL+G Sbjct: 241 LLLFGLGLVIGNLAGGKLADKKLMATLLGSLVLLAAVLVLFGLTVDNKVAAIIGVGLMGM 300 Query: 296 AAFATVPPLQMRVLEKATGAPNLASSVNIAAFNLGNALGAWLGGVTIDHGPGLHAVSLVA 355 AAFATVPPLQMRVLEKATGAPNLASS NIAAFNLGNA GAWLGGVTIDHGPGLHAV VA Sbjct: 301 AAFATVPPLQMRVLEKATGAPNLASSANIAAFNLGNAAGAWLGGVTIDHGPGLHAVPYVA 360 Query: 356 ALITAGGLVVALISWKLDRKTAPAVSLKAANKAC 389 AL+ G+VVAL+SWK+D A A +++ A Sbjct: 361 ALLPLAGIVVALVSWKMDAGRATAQHGSSSDTAA 394 >gi|300312509|ref|YP_003776601.1| arabinose efflux permease [Herbaspirillum seropedicae SmR1] Length = 385 Score = 344 bits (882), Expect = 9e-96, Method: Compositional matrix adjust. Identities = 200/381 (52%), Positives = 253/381 (66%), Gaps = 6/381 (1%) Query: 5 LWALAISAFAIGTTEFVIMGLLPEVAANLGTTLSSAGLLVTGYALGVFVGAPLVTATTGK 64 L+ALA++AF IGTTEFVIMGLLP+VA +LG T +AG+LV+GYALGV GAP++ T K Sbjct: 5 LFALAVAAFGIGTTEFVIMGLLPDVARDLGVTAPAAGMLVSGYALGVAAGAPILAILTAK 64 Query: 65 LPRKTLLVSLMVLFVIGNIGCAIAPDYTTLMISRIVASFSHGAFFGVGSVVATGLVPKEK 124 PRK LV LM LF++GN+ CA+AP+Y LM++R+V +F H AFFG+GSVVA LV +K Sbjct: 65 WPRKQALVGLMCLFILGNVLCALAPNYVFLMVARVVTAFCHAAFFGIGSVVAAELVAPQK 124 Query: 125 QASAIALMFTGLTVANVLGVPFGTWLGQMYGWRSTFWAVSAIGVVALLATAYLVPRSDDR 184 +A A+ALMFTGLTVAN+LGVP GT G GWRSTF AV+AIGV+A + ++P+ Sbjct: 125 RAQAVALMFTGLTVANILGVPLGTAFGHAAGWRSTFGAVTAIGVLAAIMLWTMLPKKIPM 184 Query: 185 QDADFRSELRVLGRPQVLLGLLMTVLGFGGVFAAFTYIAPILTDITGFQPGAVSPILLLF 244 Q + E R + +V L LL + L +F+ TYIA IL D+TGF S +LLLF Sbjct: 185 QAGNIFQEFRSIIDTRVQLSLLTSTLASISMFSVITYIAYILLDVTGFTTQQESWVLLLF 244 Query: 245 GVGLVGGNLLGGKFADKYLMPTLVISLVLLAGVLVLFSFTSHYKLVGL-----LGAAAFA 299 G G+ G L+GGK AD LMP + + +A VL LFS TSHYK L GA AFA Sbjct: 245 GAGITVGGLVGGKLADWKLMPVMCGVFLCIAVVLGLFSITSHYKWPTLATMFAWGAFAFA 304 Query: 300 TVPPLQMRVLEKATGAPNLASSVNIAAFNLGNALGAWLGGVTIDHGPGLHAVSLVAALIT 359 VP Q+RVL+ A GAPNLAS++N AFNLGNA GAW+GG+ I G L + L+ A Sbjct: 305 VVPGAQIRVLDTARGAPNLASTLNQGAFNLGNATGAWVGGLAISAGLPLTDLPLIGAFFA 364 Query: 360 AGGLVVALISWKLDRKT-APA 379 GL S LD++ APA Sbjct: 365 LLGLATVAFSASLDKRVRAPA 385 >gi|300313915|ref|YP_003778007.1| transmembrane arabinose efflux transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 403 Score = 136 bits (342), Expect = 3e-33, Method: Compositional matrix adjust. Identities = 120/397 (30%), Positives = 193/397 (48%), Gaps = 52/397 (13%) Query: 8 LAISAFAIGTTEFVIMGLLPEVAANLGTTLSSAGLLVTGYALGVFVGAPLVTATTGKLPR 67 LA+ FAIGT F+I LLP +AA+ G ++ LV + L + + +P+ T + + R Sbjct: 29 LALGTFAIGTEGFMIAPLLPRMAADFGMSVPMVASLVVIFTLVLALSSPISTVLSAAMNR 88 Query: 68 KTLLVSLMVLFVIGNIGCAIAPDYTTLMISRIVASFSHGAFFGVGSVVATGLVPKEKQAS 127 + +L++ M F N+ A + +T L+++R++ + + G + S +A +V ++ Sbjct: 89 RRVLIAAMTAFTAANLLAACSSSFTGLLVARLLMAVAAGLYTPNASALAGAMVAPGQRGR 148 Query: 128 AIALMFTGLTVANVLGVPFGTWLGQMYGWRSTFWAVSAIGVVALLATAYLVPRS--DDRQ 185 A+A++ G+T + LG+P G +G +GWR+TF AVSA+G VAL VPR Sbjct: 149 ALAIVSGGMTSPSALGLPLGAVVGHAFGWRATFLAVSAMGAVALAGILLGVPRQAGAHLH 208 Query: 186 DADFRSELRVLGRPQVLLGLLMTVLGFGGVFAAFTYIAPILTDITGFQPGAVSPILLLFG 245 A R + V+G+PQV L +T+L G +AA+ YIAP L+ + F +S + L+G Sbjct: 209 VAGLRERMAVIGQPQVQRLLAVTLLWSVGAYAAYPYIAPYLSHVLSFGESRISLTVSLWG 268 Query: 246 VGLVGGNLLGGKFADKY-------------------------LMPTLVISLVLLAGVLVL 280 G +GG D+Y L P L + VLLA V+ Sbjct: 269 AAAAVGVSVGGTLNDRYGSARVVRVSLLLLMLSFWTLAAATALPPPLALGPVLLAVVVWG 328 Query: 281 FSFTSHY--KLVGLLGAAAFATVPPLQMRVLEKATGAPNLASSVNIAAFNLGNALGAWLG 338 FS + + ++ L+GA A A P A +L +S F+LG+A GA + Sbjct: 329 FSVWAFFPAQMARLIGAGAPAQAPV-----------ALSLNTSTLYLGFSLGSAAGAGIL 377 Query: 339 GVTIDHGPGLHAVSLVAALITAGGLVVALISWKLDRK 375 G G GL A + AL++W L+R+ Sbjct: 378 GSGAIWGIGLFAGA------------CALVAWLLERR 402 >gi|300310405|ref|YP_003774497.1| arabinose efflux permease [Herbaspirillum seropedicae SmR1] Length = 422 Score = 110 bits (274), Expect = 2e-25, Method: Compositional matrix adjust. Identities = 76/236 (32%), Positives = 128/236 (54%) Query: 7 ALAISAFAIGTTEFVIMGLLPEVAANLGTTLSSAGLLVTGYALGVFVGAPLVTATTGKLP 66 +LA+ F++ T EF+ LL +A++L + +AG VT A+ V AP + T + Sbjct: 41 SLALGVFSLVTAEFLPASLLTAIASDLHVSDGAAGQAVTATAIVGAVAAPSIPLLTRNID 100 Query: 67 RKTLLVSLMVLFVIGNIGCAIAPDYTTLMISRIVASFSHGAFFGVGSVVATGLVPKEKQA 126 RK +++ L +L ++ NI AP+ L+++R++ + G F+ + + +A LVP A Sbjct: 101 RKRVVLMLSLLLLVSNILAVTAPNLPVLLVARVLLGVALGGFWSMAAALAMRLVPDHLFA 160 Query: 127 SAIALMFTGLTVANVLGVPFGTWLGQMYGWRSTFWAVSAIGVVALLATAYLVPRSDDRQD 186 A++L+ TG++VA V P G ++G ++GWRS F A + +VA+ +PR + Sbjct: 161 RAMSLILTGVSVATVCAAPIGAYMGNLWGWRSAFVAAGVVSLVAIAIQWIAIPRLPPKDH 220 Query: 187 ADFRSELRVLGRPQVLLGLLMTVLGFGGVFAAFTYIAPILTDITGFQPGAVSPILL 242 + +LGR V + LL +L G FA FTYI P++ IT +S +LL Sbjct: 221 PSLKVLAELLGRKDVRVALLAVLLVVSGHFAGFTYIRPLMEQITHMSVTTISAVLL 276 >gi|300312340|ref|YP_003776432.1| transporter transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 421 Score = 63.2 bits (152), Expect = 3e-11, Method: Compositional matrix adjust. Identities = 62/260 (23%), Positives = 108/260 (41%), Gaps = 7/260 (2%) Query: 8 LAISAFAIGTTEFVIMGLLPE-VAANLGTTLSSAGLLVTGYALGVFVGAPLVTATTGKLP 66 LA FA+ +F++M L + ++ T GL+V+ YA L + Sbjct: 27 LAFLQFAV-ILDFMLMSPLGALIMPSMQITPQQFGLVVSAYAFSAGASGLLTAGFADRFD 85 Query: 67 RKTLLVSLMVLFVIGNIGCAIAPDYTTLMISRIVASFSHGAFFGVGSVVATGLVPKEKQA 126 RK LL+ FV+G + C +A + +L+++RIV G + +AT L + Sbjct: 86 RKRLLLFFYGGFVLGTVWCGLAQSFESLLLARIVTGLFGGVIGSITLAIATDLFAPHLRG 145 Query: 127 SAIALMFTGLTVANVLGVPFGTWLGQMYGWRSTFWAVSAIGVVALLATAYLVPRSD---- 182 + ++ T + VLG+P G +L + W F A++A+G+ L + D Sbjct: 146 RVMGVIQTSFAASQVLGIPIGLYLSNRWNWHVPFLAMAALGLAGGLVVLVRMQPVDGHLT 205 Query: 183 -DRQDADFRSELRVLGRPQVLLGLLMTVLGFGGVFAAFTYIAPILTDITGFQPGAVSPIL 241 ++ + + L P+ L T L G F + + L G + I Sbjct: 206 LKQEHSAWMHLYHTLAEPRYLTAFATTALLMTGGFMLMPFSSAYLVGNLGIDMAHLPTIY 265 Query: 242 LLFGVGLVGGNLLGGKFADK 261 L+ G+ + L G+ ADK Sbjct: 266 LVTGLCTIAFGPLIGRAADK 285 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.325 0.140 0.415 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,135,159 Number of extensions: 45899 Number of successful extensions: 362 Number of sequences better than 1.0e-08: 13 Number of HSP's gapped: 364 Number of HSP's successfully gapped: 13 Length of query: 389 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 302 Effective length of database: 1,207,925 Effective search space: 364793350 Effective search space used: 364793350 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 131 (55.1 bits)