BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0005.0049.p (259 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312269|ref|YP_003776361.1| hypothetical protein Hsero_2965... 166 2e-42 >gi|300312269|ref|YP_003776361.1| hypothetical protein Hsero_2965 [Herbaspirillum seropedicae SmR1] Length = 294 Score = 166 bits (420), Expect = 2e-42, Method: Compositional matrix adjust. Identities = 94/197 (47%), Positives = 142/197 (72%), Gaps = 1/197 (0%) Query: 44 FNERYPSGSIRSTDEADEILAEADKERLIIEDQYIAEQRDCYKKFFVAACLDGAKERNRV 103 +RYP+GSI+S + A +L E ++R ++ +Y+ +QR C KF V CL+ A++R R+ Sbjct: 77 IRQRYPAGSIKSVEMAQAVLDEVAEDRQAVQLRYVLDQRACAPKFLVNKCLEEARDRKRL 136 Query: 104 AGKQVRDVEVEANAYKRQAKADDRDKSLAEQRVKDEQDSARRAADQKERDAAAARKVQES 163 A K ++ E EAN ++RQA DDRD+SLA+QR KD++D+ARR A+QK+++A +ARKVQES Sbjct: 137 AEKDLKQAEYEANVFQRQANVDDRDRSLADQRAKDQEDAARRQAEQKQKEADSARKVQES 196 Query: 164 AAKQQQVKER-EQQSEGKEDARVKAHDAQLREKQAADAAKAPQRQANEKAYQEKVKAAEV 222 AK ++V++R + + D RV+ H+A+ R+++A DAA+AP+R AN Q +++ AE Sbjct: 197 NAKNREVQQRIDANKDVPADYRVREHEAKQRQQRAEDAARAPERAANAAERQRRIQEAEQ 256 Query: 223 HRKEVETNKAEKDRERA 239 HR EVE NKAEK+RERA Sbjct: 257 HRLEVERNKAEKERERA 273 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.309 0.123 0.328 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 565,459 Number of extensions: 17434 Number of successful extensions: 163 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 171 Number of HSP's successfully gapped: 2 Length of query: 259 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 176 Effective length of database: 1,226,865 Effective search space: 215928240 Effective search space used: 215928240 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.8 bits) S2: 129 (54.3 bits)