BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0005.0045.p (377 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312271|ref|YP_003776363.1| 2-oxoglutarate dehydrogenase E3... 537 e-154 gi|300310787|ref|YP_003774879.1| 2-oxoglutarate dehydrogenase E3... 167 1e-42 gi|300312608|ref|YP_003776700.1| glutathione reductase [Herbaspi... 103 2e-23 >gi|300312271|ref|YP_003776363.1| 2-oxoglutarate dehydrogenase E3 component lipoamide dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 475 Score = 537 bits (1384), Expect = e-154, Method: Compositional matrix adjust. Identities = 276/308 (89%), Positives = 288/308 (93%) Query: 1 MSKNFDVVVIGGGPGGYIAAIRAAQLGFNTACIDEWKNEKGGPAPGGTCTNVGCIPSKAL 60 MSKNFDVVVIGGGPGGYIAAIRAAQLGFNTACIDEWKNEKGGPAPGGTCTNVGCIPSKAL Sbjct: 1 MSKNFDVVVIGGGPGGYIAAIRAAQLGFNTACIDEWKNEKGGPAPGGTCTNVGCIPSKAL 60 Query: 61 LQSSEHYEHAGHAFKDHGIDVKGLSLNLPQMLGRKNTVVKQNNDGILYLFKKNKVTFFHG 120 LQSSEHYEHA H F +HGI+VKGL LNL +MLGRKNTVVKQNNDGILYLFKKNKV+FFHG Sbjct: 61 LQSSEHYEHASHGFAEHGIEVKGLGLNLEKMLGRKNTVVKQNNDGILYLFKKNKVSFFHG 120 Query: 121 RGSFVKGDANGYEIKVAGATEESITAKQVIVATGSNARALPGAPFDEELILSNTGALAIT 180 RGSFVKGDANGYEIKVAGA EE+ITAK VIVATGSNARALPGA FDE+LILSNTGAL+IT Sbjct: 121 RGSFVKGDANGYEIKVAGAAEETITAKHVIVATGSNARALPGAEFDEKLILSNTGALSIT 180 Query: 181 EVPKKLGVIGAGVIGLEMGSVWRRLGAEVTVLEALPTFLGAVDEQIAKEAQKLLAKQGLT 240 EVPKKLGVIGAGVIGLEMGSVWRRLGAEVTVLEALPTFLGAVDEQIAKEAQKLL KQGL Sbjct: 181 EVPKKLGVIGAGVIGLEMGSVWRRLGAEVTVLEALPTFLGAVDEQIAKEAQKLLTKQGLA 240 Query: 241 VNLGVKIGDIKAGKKDVSVDYTDDKGNAQKAVFDKLIISIGRTPNTTGLNAEAIGLKLDE 300 VNLGVKIG IKAGK VSV+YTD KG+A FDKLI+SIGRTPNT GLNAE +GLKLDE Sbjct: 241 VNLGVKIGAIKAGKNSVSVEYTDAKGDAHTGEFDKLIVSIGRTPNTIGLNAEGVGLKLDE 300 Query: 301 RGFIAVNG 308 RGFIAV+G Sbjct: 301 RGFIAVDG 308 >gi|300310787|ref|YP_003774879.1| 2-oxoglutarate dehydrogenase E3 component lipoamide dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 596 Score = 167 bits (422), Expect = 1e-42, Method: Compositional matrix adjust. Identities = 104/305 (34%), Positives = 165/305 (54%), Gaps = 13/305 (4%) Query: 6 DVVVIGGGPGGYIAAIRAAQLGFNTACIDEWKNEKGGPAPGGTCTNVGCIPSKALLQSSE 65 DV+V+GGGPGGY AA RAA LG NT ++ GG C NVGCIPSKALL + Sbjct: 128 DVMVLGGGPGGYSAAFRAADLGLNTVIVEREATL------GGVCLNVGCIPSKALLHVAA 181 Query: 66 HYEHAGHAFKDHGIDVKGLSLNLPQMLGRKNTVVKQNNDGILYLFKKNKVTFFHGRGSFV 125 + A HG+ +++ ++ K+ V+ G+ + K KV +G G F Sbjct: 182 VIDETA-AMASHGVTFGKPQIDIDKLRAYKDKVIGTMTGGLAGMAKARKVQVVNGDGQF- 239 Query: 126 KGDANGYEIKVAGATEESITAKQVIVATGSNARALPGAPFDEELILSNTGALAITEVPKK 185 N E+ A +++ + K I+A GS+ LP P D I+ +TGAL + +VPK+ Sbjct: 240 -AGPNHIEVTAADGSKKVVQFKHAIIAAGSSVVNLPFVPQDPR-IVDSTGALELRQVPKR 297 Query: 186 LGVIGAGVIGLEMGSVWRRLGAEVTVLEALPTFLGAVDEQIAKEAQKLLAKQGLTVNLGV 245 + VIG G+IGLEM +V+ LGA + V+E + + D + K QK K+ V + Sbjct: 298 MLVIGGGIIGLEMATVYSTLGARIDVVEMMDGLMQGADRDMVKVWQKFNEKRFDNVMVKT 357 Query: 246 KIGDIKAGKKDVSVDYTDDKGNA---QKAVFDKLIISIGRTPNTTGLNAEAIGLKLDERG 302 K ++A + + V + + A + ++D +++++GR+PN L+A+ G+ + +RG Sbjct: 358 KTVAVEALPEGIKVTFEAAEAGATAPEPQLYDLVLVAVGRSPNGKKLSADKAGVIVSDRG 417 Query: 303 FIAVN 307 FIAV+ Sbjct: 418 FIAVD 422 >gi|300312608|ref|YP_003776700.1| glutathione reductase [Herbaspirillum seropedicae SmR1] Length = 458 Score = 103 bits (257), Expect = 2e-23, Method: Compositional matrix adjust. Identities = 93/310 (30%), Positives = 145/310 (46%), Gaps = 25/310 (8%) Query: 4 NFDVVVIGGGPGGYIAAIRAAQLGFNTACIDEWKNEKGGPAPGGTCTNVGCIPSKALLQS 63 ++D+ IGGG GG AA A+Q G EKG GGTC N+GCIP K L+ Sbjct: 5 DYDLFTIGGGSGGVRAARFASQAGARVGL-----AEKGDL--GGTCVNLGCIPKK-LMSY 56 Query: 64 SEHYEHAGHAFKDHGIDVKGL-SLNLPQMLGRKNTVVKQNNDGILYLFKKNKVTFFHGRG 122 S HY +G + G S + ++ K+ + ND L L KV+ G Sbjct: 57 SAHYHEEFADAAGYGWTLNGQPSFDWSALMANKDREIAHLNDIYLRLLDNAKVSLHRG-- 114 Query: 123 SFVKGDANGYEIKVAGATEESITAKQVIVATG--SNARALPGAPFDEELILSNTGALAIT 180 F K + + + + V G + TA+ ++VATG + A+PGA EL +++ + Sbjct: 115 -FAKVE-DAHTVNVDG---QRFTARHILVATGGRPDKPAIPGA----ELGITSDDFFHLK 165 Query: 181 EVPKKLGVIGAGVIGLEMGSVWRRLGAEVTVLEALPTFLGAVDEQIAKEAQKLLAKQGLT 240 +P++ V+G G I +E+ S+ LG EVT++ L +D + +AK+G+ Sbjct: 166 ALPQRAVVLGGGYIAVELASILNGLGCEVTLVYRRERLLRNMDADLGIHLADEMAKKGIR 225 Query: 241 VNLGVKIGDI---KAGKKDVSVDYTDDKGNAQKAVFDKLIISIGRTPNTTGLNAEAIGLK 297 + I I +AG+ + T N + ++ + GRT NT GL + G+K Sbjct: 226 IVFNASIEAIEAEQAGEASEASVKTVRLTNGEALSAQCVLFATGRTANTAGLGLQEAGVK 285 Query: 298 LDERGFIAVN 307 L G I VN Sbjct: 286 LKANGAIEVN 295 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.316 0.136 0.396 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,049,782 Number of extensions: 42109 Number of successful extensions: 206 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 201 Number of HSP's successfully gapped: 3 Length of query: 377 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 290 Effective length of database: 1,207,925 Effective search space: 350298250 Effective search space used: 350298250 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 131 (55.1 bits)