BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0005.0005.p (166 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311980|ref|YP_003776072.1| Fe-S protein [Herbaspirillum se... 102 1e-23 >gi|300311980|ref|YP_003776072.1| Fe-S protein [Herbaspirillum seropedicae SmR1] Length = 280 Score = 102 bits (254), Expect = 1e-23, Method: Compositional matrix adjust. Identities = 62/144 (43%), Positives = 77/144 (53%), Gaps = 16/144 (11%) Query: 1 MITLSALHIYPIKSCRGISLPQAAVGEFGLNLDRSWMLVDSDGHFLSQREHGRMATISTA 60 M LSA+H+YPIKSC G+SL +G GL LDR WMLVD G FL+QR+H MA I+ A Sbjct: 1 MSILSAIHLYPIKSCAGLSLTHTPIGPLGLALDRHWMLVDRQGRFLTQRQHPEMACITPA 60 Query: 61 LEHDTPALLTVRAPGMADLHLPSRIEDGAGRLAVTIWDDGVEALDCGAPLPIAGFRITCR 120 E + L +RAPGM L L + EDG LAV +W+ ++ALD G + Sbjct: 61 FEGER---LVLRAPGMPALSLSMQGEDGEA-LAVQVWNSHLDALDQG------------Q 104 Query: 121 PRCGWCVSIRRPGASAVRAGPATS 144 WC VR PA S Sbjct: 105 TAAQWCSDYLGEAVRLVRFNPAAS 128 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.325 0.138 0.472 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 613,037 Number of extensions: 26350 Number of successful extensions: 88 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 86 Number of HSP's successfully gapped: 1 Length of query: 166 Length of database: 1,619,870 Length adjustment: 78 Effective length of query: 88 Effective length of database: 1,250,540 Effective search space: 110047520 Effective search space used: 110047520 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 127 (53.5 bits)