BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0005.0004.p (153 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311981|ref|YP_003776073.1| integral membrane protein [Herb... 169 8e-44 >gi|300311981|ref|YP_003776073.1| integral membrane protein [Herbaspirillum seropedicae SmR1] Length = 149 Score = 169 bits (428), Expect = 8e-44, Method: Compositional matrix adjust. Identities = 88/150 (58%), Positives = 111/150 (74%), Gaps = 5/150 (3%) Query: 2 MLFAKYLHILGFTVWVGGMFFAYLCLRPVAAAVLQPPARLALWNGVFGKFFTWVWLAIAL 61 ML AK+ H+LG T+WVGGMFFA++ LRP A AVL P RL L V FF WVW+AIAL Sbjct: 1 MLIAKFFHLLGLTIWVGGMFFAHMALRP-ALAVLHPEQRLTLMAAVLASFFVWVWIAIAL 59 Query: 62 ILASGLPMMAQMG--KPPVYISGMAGLGILMMLIFAHLFFAPYKRLKRAVAAEDWAXAAG 119 +L SGL MMA +G P Y+ M G+G++MMLIFAH+FFAP++RLK A A +DW AA Sbjct: 60 VLGSGLHMMAMLGAGHAPPYVHAMTGIGVVMMLIFAHIFFAPFRRLKLAAAQQDWDRAA- 118 Query: 120 GAALGQIRRIVGINLILGLIVVTLGALGPL 149 ALGQ+R++V INL+LGL+ + +GALGPL Sbjct: 119 -RALGQVRQLVSINLLLGLLTIAIGALGPL 147 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.333 0.146 0.474 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 402,219 Number of extensions: 16296 Number of successful extensions: 107 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 105 Number of HSP's successfully gapped: 1 Length of query: 153 Length of database: 1,619,870 Length adjustment: 77 Effective length of query: 76 Effective length of database: 1,255,275 Effective search space: 95400900 Effective search space used: 95400900 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 39 (21.5 bits) S2: 126 (53.1 bits)