BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0004.0166.p (643 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312487|ref|YP_003776579.1| multidrug resistance transport ... 166 6e-42 gi|300312565|ref|YP_003776657.1| multidrug resistance transmembr... 155 1e-38 gi|300312486|ref|YP_003776578.1| major facilitator superfamily p... 151 2e-37 gi|300312016|ref|YP_003776108.1| multidrug resistance A (translo... 144 2e-35 gi|300312017|ref|YP_003776109.1| multidrug resistance B (translo... 129 7e-31 >gi|300312487|ref|YP_003776579.1| multidrug resistance transport transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 397 Score = 166 bits (419), Expect = 6e-42, Method: Compositional matrix adjust. Identities = 133/327 (40%), Positives = 197/327 (60%), Gaps = 5/327 (1%) Query: 8 AAGGNETNGAAPKGK----KRFIKFAVPALVLLAVAAYLVYWFAEGRHWEHTDDAYVGGD 63 A ++ G+APK + RFI A+ +VL+ VAA+++Y+ G++ E T+DA+V D Sbjct: 18 APRSDDKQGSAPKKRMNPRARFILRALLVVVLVIVAAWVLYYQFRGKYLESTNDAFVQAD 77 Query: 64 ITVIAPKVPGNIVKVLVADNQRVKAGDLLVKIDDRDYVAVLNKLDAAVQAQQAALANIDA 123 ++ KV G + +VLVADNQ VKAG LV+ID RDY A + A + A A+ +A Sbjct: 78 SVTVSSKVSGYVDQVLVADNQIVKAGQPLVRIDARDYSAQAAQAKAQIDAAAASEEAANA 137 Query: 124 ERRLQEAVIEQGKAGVAASNADLTRTRDDQTRYQSLSSRAAVSVQSFQKADADYKQAQAN 183 + Q+A I Q KA +AA++ D + RY L++ A + + QA++ Sbjct: 138 QLAEQQAAIAQAKAQLAAADEDARFATAEVERYTPLAASGAETRERLTTLHNQQAQARST 197 Query: 184 GQKAQAGLLAAQRQLDVLATQKQKVQAAMQQAIAERNLAQLNVGYTELRAPVDGVVGNRR 243 +A L AAQ ++ L Q ++ QA + A A+ + A +N+GYTE+RA VDG +G+++ Sbjct: 198 VAARRAALDAAQLRVRSLQAQVRQAQAQGETAKAQYDAASVNLGYTEIRASVDGRIGDKQ 257 Query: 244 ARTGAYAATGAQLLSVVPAEGLWVDANFKESQIAHFQSGMTVKIKADVLPGETFQGHVAS 303 R G + + G ++++VVP +V ANFKE+QI + G IK D LPG F GHV S Sbjct: 258 VRVGQFVSPGTRMMTVVPTR-FYVSANFKETQIGIMRIGQPAIIKVDALPGVEFHGHVES 316 Query: 304 IAPATGAQFSILPAENATGNFTKIVQR 330 ++P TGAQFS+LP +NATGNFTKIVQR Sbjct: 317 LSPGTGAQFSLLPPQNATGNFTKIVQR 343 >gi|300312565|ref|YP_003776657.1| multidrug resistance transmembrane efflux pump protein [Herbaspirillum seropedicae SmR1] Length = 411 Score = 155 bits (392), Expect = 1e-38, Method: Compositional matrix adjust. Identities = 121/350 (34%), Positives = 183/350 (52%), Gaps = 24/350 (6%) Query: 3 TQANAAAGGNETNGAAPKGKKRFIKFAV-PALVLLAVAAYLVYWFAEGRHWEHTDDAYVG 61 T A A NE AA +KR F++ +V +A Y YW+ G + TD+AY Sbjct: 11 TLAEEAKQRNEAASAAAAAQKRKKLFSIFGGVVAIAAIGYGAYWYLIGSRYVETDNAYTA 70 Query: 62 GDITVIAPKVPGNIVKVLVADNQRVKAGDLLVKIDDRDY-------VAVLNKLDAAVQAQ 114 +I + P + G + V V D Q VK GD+LV+IDD D A L++ + V+ Sbjct: 71 TEIATVTPAINGIVAAVDVVDTQAVKKGDVLVRIDDADARLAVDQAAADLDRTERKVKGF 130 Query: 115 QAALANIDAERRLQEAVIEQGKAGVAASNADLTRTRDDQTRYQSLSSRAAVSVQSFQKAD 174 A A + A+ +EA ++ A + ++ ADL R D R ++L+ +VS + A Sbjct: 131 FANDAGLAAQVLAREAEQKRASAQLLSAQADLKRAEIDLQRREALAKSGSVSGEELSNAR 190 Query: 175 ADYKQAQANGQKAQAGLLAAQRQLDVLATQ-KQKVQAAMQQ------------AIAERNL 221 AQAN + A+A + Q + ++ ATQ QK + A A+ Sbjct: 191 TALLTAQANLKAAEAAEV--QSRANIKATQGAQKASTVLTANTTVDDNPEVVLARAKLEQ 248 Query: 222 AQLNVGYTELRAPVDGVVGNRRARTGAYAATGAQLLSVVPAEGLWVDANFKESQIAHFQS 281 A+L++ T LRAPVDGV+ R+ + G +GA LLSVVP + + VDANFKE Q+ + Sbjct: 249 AKLDLERTVLRAPVDGVIARRQVQVGQRVQSGATLLSVVPLQQMHVDANFKEGQLTKVRI 308 Query: 282 GMTVKIKADVLPGE-TFQGHVASIAPATGAQFSILPAENATGNFTKIVQR 330 G V +KAD+ G + G V ++ TG+ F+++PA+NATGN+ K+VQR Sbjct: 309 GQPVTMKADLYGGSVEYHGVVTGLSGGTGSAFAVIPAQNATGNWIKVVQR 358 >gi|300312486|ref|YP_003776578.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 514 Score = 151 bits (381), Expect = 2e-37, Method: Compositional matrix adjust. Identities = 94/275 (34%), Positives = 146/275 (53%), Gaps = 9/275 (3%) Query: 351 FTIASMLCGLAWDINSMIFFRALQGFLGGSMIPMVFTSAFFYFSGHQRVIAAATVGALSS 410 FT+ S+LCGL+ + MI R QGF GG++IP T Q+ + A GA + Sbjct: 92 FTLFSVLCGLSTTLPMMIVGRVGQGFTGGALIPTAMTIIATRLPRSQQPVGNAMFGATAI 151 Query: 411 LAPTLGPTVGGWITSNYSWHWLFFINLAPGIFVAVVVPMLVRIDKPNLSLLRGADYLGMI 470 L P LGP +GGW+T N SWH+ FFINL G + ++ + + ++P L AD+ G+ Sbjct: 152 LGPVLGPVMGGWLTENVSWHYAFFINLPVGAALITLLLVAIPYERPKFEQLAEADWFGIA 211 Query: 471 LLAASLGCLEYSLEEGPRLGWMGDDIIRTTVWIAIVAGVGFIWRSLAY---DNPVVDLRA 527 LA LG L LEEG R W IR W+A + +GFI ++ +PV+ LR Sbjct: 212 GLALGLGGLTVVLEEGEREQWFSSPDIR---WLAATSVLGFILLAIGQVRARHPVIQLRL 268 Query: 528 LKDRNFALGCFFSFVTGIGIFATVYLTPVFLAHVRGFSALDIGLAIFATGLFQICAIPFY 587 L+DR F + + G+ ++ T Y+ P FL+ + G+++L G + +G+ I +PF Sbjct: 269 LRDRQFGSVVMMAMIVGVALYGTAYVIPQFLSGIAGYNSLQSGWIVLLSGVPMILMMPFT 328 Query: 588 TYLGRRVDLRWLMMFGMACFALSMW---NFTPITH 619 L R VD+R + G A S + + +P+++ Sbjct: 329 PLLMRLVDIRIAVGTGFLLLAASAFIETDLSPLSY 363 >gi|300312016|ref|YP_003776108.1| multidrug resistance A (translocase) transmembrane secretion protein [Herbaspirillum seropedicae SmR1] Length = 406 Score = 144 bits (363), Expect = 2e-35, Method: Compositional matrix adjust. Identities = 125/335 (37%), Positives = 174/335 (51%), Gaps = 27/335 (8%) Query: 2 TTQANAAAGGNETNGAAPKGKKRFIKFAVPALVLLAVAAYLVYWFAEGRHWEHTDDAYVG 61 TTQ A N NG +KR + L+++AVA +L YWF R +E TDDAYVG Sbjct: 5 TTQQQPQAN-NNGNGK----RKRQLILLTLVLLVIAVACFL-YWFLHARFFEETDDAYVG 58 Query: 62 GDITVIAPKVPGNIVKVLVADNQRVKAGDLLVKIDDRDYVAVLNKLDAAV-----QAQQA 116 G++ I+ +V G +V V D Q VKAG LV +D D L++ AA+ Q +Q Sbjct: 59 GNVVQISAQVGGTVVAVKADDTQVVKAGQQLVALDAADTRLALDQAQAALAQAVRQTRQL 118 Query: 117 ALANIDAERRLQEAVIEQGKAGVAASNADLTRTRDDQTRYQSLSSRAAVSVQSFQKADAD 176 L N + A VAA++++L R R+D R Q+ S AVS + A Sbjct: 119 FLNN------------DTLAANVAAADSNLARAREDLQRRQAGLSSGAVSQEDVSHARDA 166 Query: 177 YKQAQANGQKAQAGLLAAQRQLDVLATQKQKVQAAMQQAIAERNLAQLNVGYTELRAPVD 236 K A A +A+A A + D + + +Q A A RN A LN + APV Sbjct: 167 VKSAVAALDQARAAAAANRALTDHTSVTEHP--NVLQAATAVRN-AYLNYARVNIVAPVS 223 Query: 237 GVVGNRRARTGAYAATGAQLLSVVPAEGLWVDANFKESQIAHFQSGMTVKIKADVLPGE- 295 G V R + G A G L+++VP E +W+DANFKESQ+ H + G V++ ADV Sbjct: 224 GFVSKRSVQVGQRIAAGNPLMAIVPLEQIWIDANFKESQLQHIRIGQPVEVIADVYGSSV 283 Query: 296 TFQGHVASIAPATGAQFSILPAENATGNFTKIVQR 330 ++G V + TG FS+LPA+NATGN+ K+VQR Sbjct: 284 KYKGTVIGFSAGTGGAFSLLPAQNATGNWIKVVQR 318 >gi|300312017|ref|YP_003776109.1| multidrug resistance B (translocase) transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 524 Score = 129 bits (324), Expect = 7e-31, Method: Compositional matrix adjust. Identities = 90/287 (31%), Positives = 139/287 (48%), Gaps = 5/287 (1%) Query: 328 VQRXGWLARVMSTRWLFSASAVGFTIASMLCGLAWDINSMIFFRALQGFLGGSMIPMVFT 387 + GWL + + LF S + F +AS LCGLA + ++ R LQG + G M+P+ + Sbjct: 76 IPLTGWLTQRLGAVRLFVTSVLLFVLASWLCGLAPTLPILLAARVLQGLVAGPMVPLSQS 135 Query: 388 SAFFYFSGHQRVIAAATVGALSSLAPTLGPTVGGWITSNYSWHWLFFINLAPGIFVAVVV 447 + + A A G + +AP GP +GGWIT +Y+W W+F+IN+ G+ A V Sbjct: 136 LLLGAYPKSKSSFALALWGMTAVVAPVAGPALGGWITDSYTWPWIFYINIPVGLIAAGVT 195 Query: 448 PMLVRI-DKPNLSLLRGADYLGMILLAASLGCLEYSLEEGPRLGWMGDDIIRTTVWIAIV 506 + R + P L D +G+ LL + + L++G L W I T IA++ Sbjct: 196 LAIYRSRETPTHKL--PIDKIGLALLVTWVAAFQIMLDKGKDLDWFASPTIVTLGIIALI 253 Query: 507 AGVGFIWRSLAYDNPVVDLRALKDRNFALGCFFSFVTGIGI-FATVYLTPVFLAHVRGFS 565 A F+ L D+PVVDLR RNFA G + G G+ F + + P +L G+ Sbjct: 254 AFAYFVVWELNNDHPVVDLRLFGRRNFA-GGTIAISIGYGVFFGNLVILPQWLQQYLGYR 312 Query: 566 ALDIGLAIFATGLFQICAIPFYTYLGRRVDLRWLMMFGMACFALSMW 612 ++D GL+ G+F + +P VD R + FA+ W Sbjct: 313 SIDSGLSTAPIGIFAVILMPLIGRFLPLVDARKVATASFIGFAIVFW 359 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.324 0.136 0.418 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,679,187 Number of extensions: 66136 Number of successful extensions: 441 Number of sequences better than 1.0e-08: 18 Number of HSP's gapped: 416 Number of HSP's successfully gapped: 18 Length of query: 643 Length of database: 1,619,870 Length adjustment: 91 Effective length of query: 552 Effective length of database: 1,188,985 Effective search space: 656319720 Effective search space used: 656319720 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 134 (56.2 bits)