BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0004.0162.p (133 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313901|ref|YP_003777993.1| hypothetical protein Hsero_4619... 191 2e-50 >gi|300313901|ref|YP_003777993.1| hypothetical protein Hsero_4619 [Herbaspirillum seropedicae SmR1] Length = 315 Score = 191 bits (484), Expect = 2e-50, Method: Compositional matrix adjust. Identities = 95/125 (76%), Positives = 102/125 (81%) Query: 1 MEALRTRIEASVISISRPPGSPDFRLPKGDPGLFGPDSACWQVHSDFTSMLIGGIGALLL 60 ME LR RIEA+V+SISR PGS DF P GDPGLFGPDSA WQVHSDFT+ML GG+ ALLL Sbjct: 24 MEFLRQRIEATVLSISRGPGSADFLQPPGDPGLFGPDSAAWQVHSDFTAMLTGGVAALLL 83 Query: 61 QMLHPLPLAGVWDHSTFQQDMLGRLRGTAQFIAGTTFGSSADAARLIERVRRIHAGVRAR 120 QMLHPLPLAGVWDHS FQ DMLGRLR TAQF+AGTTFGS ADA RLI RV++IH GV Sbjct: 84 QMLHPLPLAGVWDHSNFQADMLGRLRRTAQFVAGTTFGSRADAERLIARVKQIHLGVNGV 143 Query: 121 RTDAR 125 D R Sbjct: 144 AADGR 148 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.325 0.140 0.437 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 413,412 Number of extensions: 14834 Number of successful extensions: 59 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 59 Number of HSP's successfully gapped: 1 Length of query: 133 Length of database: 1,619,870 Length adjustment: 75 Effective length of query: 58 Effective length of database: 1,264,745 Effective search space: 73355210 Effective search space used: 73355210 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 125 (52.8 bits)