BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0004.0161.p (364 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313900|ref|YP_003777992.1| cation/multidrug efflux pump pr... 631 0.0 gi|300310853|ref|YP_003774945.1| cation/multidrug efflux pump pr... 107 2e-24 gi|300311798|ref|YP_003775890.1| cation/multidrug efflux pump pr... 99 4e-22 gi|300310852|ref|YP_003774944.1| cation/multidrug efflux pump tr... 87 3e-18 gi|300309819|ref|YP_003773911.1| acriflavin resistance transmemb... 85 1e-17 >gi|300313900|ref|YP_003777992.1| cation/multidrug efflux pump protein [Herbaspirillum seropedicae SmR1] Length = 1063 Score = 631 bits (1628), Expect = 0.0, Method: Compositional matrix adjust. Identities = 308/354 (87%), Positives = 337/354 (95%) Query: 1 MSMVELALRRPYTFIVMAMLIVLSTPLVLMKMATDIFPEINIPVISIIWNYNGLSAQEMG 60 M+MV LALRRPYTFIVMA+LIVL+TPL L MATDIFPEINIPV+SIIWNYNGLSAQEMG Sbjct: 1 MNMVALALRRPYTFIVMALLIVLATPLTLKNMATDIFPEINIPVVSIIWNYNGLSAQEMG 60 Query: 61 QRIASQNERGLTTVVSDIEHIESQSLAGVAVIKVFFQPTANIQNAIAQTVASVQSQLRQL 120 QRIA+QNERGLTTVVSDIEHIESQSLAGV+VIKVFFQP ANIQ AIAQ VASVQ+Q+RQL Sbjct: 61 QRIAAQNERGLTTVVSDIEHIESQSLAGVSVIKVFFQPKANIQTAIAQVVASVQTQVRQL 120 Query: 121 PPGITPPLVIKYSASSIPVMQLALSSPTLPEQSLFDAAINTLRPQLITIPGVAVPFPYGG 180 PPGITPPLVIKYSASSIPVMQLALSSPTLPEQ++FDAA+ TLRPQL+TIPGVAVP+PYGG Sbjct: 121 PPGITPPLVIKYSASSIPVMQLALSSPTLPEQTVFDAAVQTLRPQLVTIPGVAVPYPYGG 180 Query: 181 KTRLISVDLDTQALQARGLSPVDVVNAVNTQNLILPSGTAKFGGTEYTVKMNGSPEAVAG 240 K+RLISVDLDTQALQARGL+P+DVVNA+NTQNLILPSGTAKFG TEY+VK+NGSPEAVAG Sbjct: 181 KSRLISVDLDTQALQARGLAPIDVVNAINTQNLILPSGTAKFGATEYSVKINGSPEAVAG 240 Query: 241 LNDLPVRTSNGATTYLRDVAYVRDGFSPQTNVVRQDGVRGVLLSVLKNGGASTLDIVNNL 300 LN+LPVRT+NGAT YL+DVA VRDGFSPQTNVVRQDG RGVLLS+LKNGGASTLDIV+NL Sbjct: 241 LNELPVRTTNGATIYLKDVANVRDGFSPQTNVVRQDGARGVLLSILKNGGASTLDIVDNL 300 Query: 301 RALLPQAQQTMPSDVKITPLFDQSLFVKAAVKGVVAEALIAAGLTAAMVLLFLG 354 R LLP A QT+P D+KITPLFDQS+FVKAAV GV++EALIAAGLTAAMVLLFLG Sbjct: 301 RKLLPTAAQTLPEDIKITPLFDQSVFVKAAVTGVISEALIAAGLTAAMVLLFLG 354 >gi|300310853|ref|YP_003774945.1| cation/multidrug efflux pump protein [Herbaspirillum seropedicae SmR1] Length = 1078 Score = 107 bits (266), Expect = 2e-24, Method: Compositional matrix adjust. Identities = 87/347 (25%), Positives = 164/347 (47%), Gaps = 2/347 (0%) Query: 8 LRRPYTFIVMAMLIVLSTPLVLMKMATDIFPEINIPVISIIWNYNGLSAQEMGQRIASQN 67 ++RP ++ + I L+ + + P+++ P ISI + G S + M +A+ Sbjct: 8 IQRPIATTLLTIGIALAGIVAFRLLPVSPLPQVDYPTISISASMPGASPETMAATVATPL 67 Query: 68 ERGLTTVVSDIEHIESQSLAGVAVIKVFFQPTANIQNAIAQTVASVQSQLRQLPPGI-TP 126 ER + ++ + + S S I + F + +I A A++ + LP G+ + Sbjct: 68 ERAMG-AIAGVTEMTSTSSQNSTRITLQFDLSRDIDGAARDVQAALNAARNLLPTGMPSN 126 Query: 127 PLVIKYSASSIPVMQLALSSPTLPEQSLFDAAINTLRPQLITIPGVAVPFPYGGKTRLIS 186 P K + + P++ LA++S T+ + ++DAA + + +L + GV G + Sbjct: 127 PTYRKVNPADAPILILAMTSDTMTQGQMYDAADSIVAQKLSQVRGVGQVSVGGSSQPAVR 186 Query: 187 VDLDTQALQARGLSPVDVVNAVNTQNLILPSGTAKFGGTEYTVKMNGSPEAVAGLNDLPV 246 ++L+ AL G+ DV AV N P G + + + N + A L V Sbjct: 187 IELNPTALNKYGIGTADVRTAVAATNANRPKGLLEDQDRNWQIYANDQAKTAAEYAPLIV 246 Query: 247 RTSNGATTYLRDVAYVRDGFSPQTNVVRQDGVRGVLLSVLKNGGASTLDIVNNLRALLPQ 306 NG ++DVA VRD + N +G VL+ + + GA+ +D V+N+R LLPQ Sbjct: 247 SYRNGTPVRVQDVAEVRDSVADVRNAGSSNGKPSVLIIINRQPGANIIDTVDNVRELLPQ 306 Query: 307 AQQTMPSDVKITPLFDQSLFVKAAVKGVVAEALIAAGLTAAMVLLFL 353 Q ++PS + + + D++ ++A++K +++ L +V LFL Sbjct: 307 LQASIPSAIDMKVVLDRTPTIRASLKETEFTLILSVALVIMVVFLFL 353 >gi|300311798|ref|YP_003775890.1| cation/multidrug efflux pump protein [Herbaspirillum seropedicae SmR1] Length = 1051 Score = 99.4 bits (246), Expect = 4e-22, Method: Compositional matrix adjust. Identities = 87/368 (23%), Positives = 163/368 (44%), Gaps = 18/368 (4%) Query: 1 MSMVELALRRPYTFIVMAMLIVLSTPLVLMKMATDIFPEINIPVISIIWNYNGLSAQEMG 60 MS+ ++RP ++A+ I+L V + P+++ P I + G S + M Sbjct: 1 MSVSAAFIKRPIGTTLLAIAILLVGVAVWPLLPVAPLPQVDFPTIQVTAQLPGGSPETMA 60 Query: 61 QRIASQNERGLTTVVSDIEHIESQSLAGVAVIKVFFQPTANIQNAIAQTVASVQSQLRQL 120 +A ER + +++ + + SQS G I + F NI A A++ + QL Sbjct: 61 SNVAQPLERQFS-LIAGLSQMTSQSTLGSTQITLQFDLNRNIDAAALDVQAAINASTGQL 119 Query: 121 PPGI-TPPLVIKYSASSIPVMQLALSSPTLPEQSLFDAAINTLRPQLITIPGVAVPFPYG 179 P + PP K + + P+M +++ S LP + D A N L Q+ I GV + G Sbjct: 120 PANLPNPPTFRKINPADSPIMIMSVQSEALPLTVVNDYADNILAQQISQISGVGLVNVNG 179 Query: 180 GKTRLISVDLDTQALQARGLSPVDVVNAVNTQNLILPSGTAKFGGTEYTVKMNGSPEAVA 239 + + + +D L GLS D+ + T + P GT +TV N Sbjct: 180 QQKPAVRIQVDPAKLATLGLSLEDIRGVIATTTVNQPKGTVDGARQAFTVYTNDQLLKAE 239 Query: 240 GLNDLPVRTSNGATTYLRDVAYVRDGFSPQTNVVRQDGV--------------RGVLLSV 285 ND+ + NGA ++D+ DG P+ N + R ++L++ Sbjct: 240 QWNDMVLAWRNGAPIRVKDIGIAVDG--PENNKIAAWAFAGAANTADHSITNGRSIVLAI 297 Query: 286 LKNGGASTLDIVNNLRALLPQAQQTMPSDVKITPLFDQSLFVKAAVKGVVAEALIAAGLT 345 K GA+ ++ V ++A +P+ + +P+ + + L D++ ++A+V+ V ++ L Sbjct: 298 TKQPGANVIETVGRIKAAMPRLRAAIPAAIHVNTLIDRTQTIRASVEDVEFTLVLTIALV 357 Query: 346 AAMVLLFL 353 ++ +FL Sbjct: 358 VMVIFVFL 365 >gi|300310852|ref|YP_003774944.1| cation/multidrug efflux pump transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 1048 Score = 86.7 bits (213), Expect = 3e-18, Method: Compositional matrix adjust. Identities = 79/342 (23%), Positives = 164/342 (47%), Gaps = 4/342 (1%) Query: 13 TFIVMAMLIVLSTPLVLMKMATDIFPEINIPVISIIWNYNGLSAQEMGQRIASQNERGLT 72 + +++A+L+V L+ ++ PE++ P I ++ Y G S M + + ER Sbjct: 15 SLLMLAILLVGIVAYRLLPLSA--LPEVDYPTIQVVTLYPGASPDVMTSSVTAPLERQFG 72 Query: 73 TVVSDIEHIESQSLAGVAVIKVFFQPTANIQNAIAQTVASVQSQLRQLPPGI-TPPLVIK 131 + ++ + S S G +VI + F ++ A + A++ + LP + PP+ K Sbjct: 73 QM-PGLKQMSSTSSGGASVITLQFNLDLSLDIAEQEVQAAINAGSNLLPSDLPMPPVYSK 131 Query: 132 YSASSIPVMQLALSSPTLPEQSLFDAAINTLRPQLITIPGVAVPFPYGGKTRLISVDLDT 191 + + P++ LA++S T+P + D + ++ + GV + GG+ + + L+ Sbjct: 132 VNPADTPIITLAVTSNTMPLPKVQDLIDTRIAQKISQLSGVGLVSLSGGQRPAVRMQLNP 191 Query: 192 QALQARGLSPVDVVNAVNTQNLILPSGTAKFGGTEYTVKMNGSPEAVAGLNDLPVRTSNG 251 +A+ A GL+ DV A+ N+ G+ T+ N + +L + NG Sbjct: 192 RAVAALGLNLDDVRTAIGNANVNQAKGSFDGPTRASTIDANDQLRSADEYRNLIIAYKNG 251 Query: 252 ATTYLRDVAYVRDGFSPQTNVVRQDGVRGVLLSVLKNGGASTLDIVNNLRALLPQAQQTM 311 A + DVA V DG + V++++ + GA+ + +V++++ +LPQ Q ++ Sbjct: 252 APIRVSDVADVVDGAENVRLGAWANTQPAVIVNIQRQPGANVIAVVDSIKKILPQLQASL 311 Query: 312 PSDVKITPLFDQSLFVKAAVKGVVAEALIAAGLTAAMVLLFL 353 P + + L D++ ++A+V V E L++ L ++ +FL Sbjct: 312 PGAIDVHILTDRTTTIRASVDDVKFELLLSVALVVMVIFVFL 353 >gi|300309819|ref|YP_003773911.1| acriflavin resistance transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 1048 Score = 84.7 bits (208), Expect = 1e-17, Method: Compositional matrix adjust. Identities = 104/369 (28%), Positives = 165/369 (44%), Gaps = 28/369 (7%) Query: 3 MVELALRRPYTFIVMAMLIVLSTPLVLMKMATDIFPEINIPVISIIWNYNGLSAQEMGQR 62 M + + RP V+A+ I+L+ L + + +P I P I + Y G +AQ + Sbjct: 1 MGKFFIDRPVFAWVLAIFIMLAGALAITGLPVSQYPNIAPPTIVVTATYPGATAQTLDDS 60 Query: 63 IASQNERGLTTVVSDIEHIESQSLA-GVAVIKVFFQPTANIQNAIAQTVASVQSQLRQ-- 119 + S E+ + +++IESQS A G I + F P N A VQ++L++ Sbjct: 61 VTSLIEQEMNGA-DGLQYIESQSQANGQVQITITFSPETNPDLA----AVDVQNRLKRVE 115 Query: 120 --LPPGITPPLVIKYSASSIPVMQLALSSP--TLPEQSLFDAAINTLRPQLITIPGVAVP 175 LP +T V + S ++ + LSS L +L D + ++ +PGV Sbjct: 116 ARLPAAVTQQGVQVTKSRSNFLLFIGLSSTDGKLDPVALGDYLSRNVLNEIKRVPGVGQA 175 Query: 176 FPYGGKTRLISVDLDTQALQARGLSPVDVVNAVNTQNLILPSGTAKFG-----GTEY--- 227 +G + R + + +D L L+P DV A+ QN ++ GT G GT+ Sbjct: 176 QLFGTE-RAMRIWIDPNKLIGLNLTPADVTAAIQAQNALVAGGT--LGALPSPGTQQIAA 232 Query: 228 TVKMNG---SPEAVAGLNDLPVRTSNGATTYLRDVAYVRDGFSPQTNVVRQDGVRGVLLS 284 TV +NG SPE N + +NGAT LRDVA V G R DG + Sbjct: 233 TVVVNGQLTSPEQFG--NIILRANANGATVRLRDVARVEVGGQSYDTSGRLDGKPSSFIG 290 Query: 285 VLKNGGASTLDIVNNLRALLPQAQQTMPSDVKITPLFDQSLFVKAAVKGVVAEALIAAGL 344 V + A+ L + + + + P+ VK T +D S FVK +++ VV + A L Sbjct: 291 VQLSPTANALGTATAVHKKMEELAKYFPTGVKYTIPYDTSKFVKISIEEVVKTLIEAIVL 350 Query: 345 TAAMVLLFL 353 ++ LFL Sbjct: 351 VFLVMFLFL 359 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.134 0.372 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,014,118 Number of extensions: 40749 Number of successful extensions: 177 Number of sequences better than 1.0e-08: 11 Number of HSP's gapped: 161 Number of HSP's successfully gapped: 11 Length of query: 364 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 278 Effective length of database: 1,212,660 Effective search space: 337119480 Effective search space used: 337119480 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 131 (55.1 bits)