BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0004.0160.p (522 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313900|ref|YP_003777992.1| cation/multidrug efflux pump pr... 701 0.0 gi|300313716|ref|YP_003777808.1| cobalt-zinc-cadmium resistance ... 105 9e-24 gi|300311798|ref|YP_003775890.1| cation/multidrug efflux pump pr... 97 4e-21 gi|300310690|ref|YP_003774782.1| cation/multidrug efflux pump pr... 93 4e-20 gi|300313244|ref|YP_003777336.1| multidrug efflux system transme... 92 1e-19 >gi|300313900|ref|YP_003777992.1| cation/multidrug efflux pump protein [Herbaspirillum seropedicae SmR1] Length = 1063 Score = 701 bits (1809), Expect = 0.0, Method: Compositional matrix adjust. Identities = 338/400 (84%), Positives = 365/400 (91%), Gaps = 7/400 (1%) Query: 1 VTIENIERHMHMGKPLHDAIIVGAGEIGVPAFVSTVCICIVFVPMFFLSGVARFLFVPLA 60 VTIENIERH+H+GKPLHDAIIVGAGEIGVPAFV+T+CICIVFVPMFFLSGVARFLFVPLA Sbjct: 405 VTIENIERHLHLGKPLHDAIIVGAGEIGVPAFVATLCICIVFVPMFFLSGVARFLFVPLA 464 Query: 61 EAVVFAMAASYILSRTLVPTLVMLLMGGVHGAEGGNSGGKTSLLQRLYRGFDSRFEHVRR 120 EAVVFAMAASY+LSRTLVPTLVMLLMG + G K SLLQRLYR FD+RFE VR Sbjct: 465 EAVVFAMAASYLLSRTLVPTLVMLLMG----SHGQVDSAKQSLLQRLYRAFDNRFERVRH 520 Query: 121 AYTLVLSSLLSHRRRFALAFLGFCVLSCLLYPFLGRDFFPNVDGGQIRLHMRMPTGTRIE 180 YTLVLSSLL+HRRRFALAFLGFC+LSCLLYPFLGRDFFPNVDGGQIRLHMRMPTGTRIE Sbjct: 521 GYTLVLSSLLAHRRRFALAFLGFCLLSCLLYPFLGRDFFPNVDGGQIRLHMRMPTGTRIE 580 Query: 181 ETARAADQVEAAIREMIPAHELETILDNIGVPNSGINLSYSNAGTIGTLDGEILMSLHED 240 ETARAAD+VE IR +IPA ELETILDN+GVPNSGINLSYSNAGTIGTLDGEILM+L E Sbjct: 581 ETARAADEVERVIRTLIPASELETILDNLGVPNSGINLSYSNAGTIGTLDGEILMALKEG 640 Query: 241 HRPTEQLVSMLRAELPKRFPGIEFFFQPADIVTQILNFGLPAAIDVQFTGPNLQQNAELA 300 HRPTE LVSMLRAELP+RFPGIEFFFQPADIVTQILNFGLPAAIDVQFTGPN++ NA LA Sbjct: 641 HRPTEALVSMLRAELPRRFPGIEFFFQPADIVTQILNFGLPAAIDVQFTGPNMEANARLA 700 Query: 301 AQLTKQIRQIPGAVDAHVHQRLDGPALDMQMDRTRLQQFGLSAANVGQNVLIALSGSSQT 360 A+LTKQIRQIPGAVDAHVHQRLDGP+LD++MDRTRLQQFGLSA+NVGQN+LIAL+GSSQT Sbjct: 701 AELTKQIRQIPGAVDAHVHQRLDGPSLDLRMDRTRLQQFGLSASNVGQNLLIALAGSSQT 760 Query: 361 TPAFWLNPQNGVVYNIAVQSPQYRTGT---TLSVPALTGA 397 PAFWLNP NGVVYNIAVQSPQY+ + L++P GA Sbjct: 761 QPAFWLNPNNGVVYNIAVQSPQYQVDSLDALLNIPVSPGA 800 Score = 229 bits (583), Expect = 5e-61, Method: Compositional matrix adjust. Identities = 118/133 (88%), Positives = 124/133 (93%) Query: 383 YRTGTTLSVPALTGAIMTMGVATANSILIVSFARQRREEGATPLAAALESGATRIRPVLM 442 + TGTTLSVPALTGAIMTMGVATANSILIV+FARQRREEGATPLAAALE+G+TRIRPVLM Sbjct: 929 FITGTTLSVPALTGAIMTMGVATANSILIVAFARQRREEGATPLAAALEAGSTRIRPVLM 988 Query: 443 TALAMIIGMIPMALGLGEGAEQNAPLGRAVIGGLIFATVSTLFFVPVVFAGVHQRLARRK 502 TALAMIIGMIPMALGLGEGAEQNAPLGRAVIGGL+ ATVSTLFFVPVVFAGVHQRLARR Sbjct: 989 TALAMIIGMIPMALGLGEGAEQNAPLGRAVIGGLMLATVSTLFFVPVVFAGVHQRLARRA 1048 Query: 503 EARRQDGRGPADQ 515 + RR GPA Q Sbjct: 1049 DQRRALAGGPAHQ 1061 >gi|300313716|ref|YP_003777808.1| cobalt-zinc-cadmium resistance cation efflux system transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 1073 Score = 105 bits (262), Expect = 9e-24, Method: Compositional matrix adjust. Identities = 89/378 (23%), Positives = 174/378 (46%), Gaps = 44/378 (11%) Query: 1 VTIENIERHM-----HMGKPLHDA-----IIVGAGEIGVPAFVSTVCICIVFVPMFFLSG 50 V +EN R + H G+ L + + A E P + I IV++PMF L+G Sbjct: 412 VIVENCVRRLAHAQAHHGRTLTRSERFHEVFAAAKEARRPLIFGQLIIMIVYLPMFALTG 471 Query: 51 VARFLFVPLAEAVVFAMAASYILSRTLVPTLVMLLMGGVHGAEGGNSGGKTSLLQRLYRG 110 V +F P+A VV A+ + ILS T +P + L +G +++ Sbjct: 472 VEGKMFTPMAFTVVAALIGAMILSVTFIPAAIALFIG-----------------EKVAEK 514 Query: 111 FDSRFEHVRRAYTLVLSSLLSHRRRFALAFLGFCVLSC-LLYPFLGRDFFPNVDGGQIRL 169 + +R Y +L +++ + + L F G +L C ++ LG +F P+++ G + + Sbjct: 515 ENILMLWAKRLYEPMLKTIMEN-KPVVLTFAGALILVCGVVASRLGSEFVPSLNEGDMAI 573 Query: 170 H-MRMPTGTRIEETARAADQVEAAIREMIPAHELETILDNIGVPNSGINLSYSNAGTIGT 228 +R+P GT + ++ Q+E +++ P E+E + G + N Sbjct: 574 QALRIP-GTSLSQSLEMQKQIEKTLKQKFP--EVERVFARTGTAEVASDPMPPNIS---- 626 Query: 229 LDGEILMSLHED----HRPTEQLVSMLRAELPKRFPGIEF-FFQPADIVTQILNFGLPAA 283 DG I++ E + E+L+ ++ E+ K PG + F QP + L G+ + Sbjct: 627 -DGYIMLKPQEQWPEPKKSREELLEAIQDEVAK-IPGNSYEFSQPIQLRFNELISGVRSD 684 Query: 284 IDVQFTGPNLQQNAELAAQLTKQIRQIPGAVDAHVHQRLDGPALDMQMDRTRLQQFGLSA 343 + V+ G +++ + A++++ + +IPGA + + Q P L +++DR + ++GL+ Sbjct: 685 VAVKVFGDDMKVLNDTASKISAVLEKIPGATEVKIEQTTGLPMLTVEIDREKTARYGLNV 744 Query: 344 ANVGQNVLIALSGSSQTT 361 +++ V A+ G T Sbjct: 745 SDIQDTVATAIGGKEAGT 762 Score = 83.2 bits (204), Expect = 5e-17, Method: Compositional matrix adjust. Identities = 55/132 (41%), Positives = 73/132 (55%), Gaps = 6/132 (4%) Query: 386 GTTLSVPALTGAIMTMGVATANSILIVSFARQRREEGATPLAAALESGATRIRPVLMTAL 445 G +S+ A G I GVA N ++++SF R REEG T AA E TR+RPVLMTAL Sbjct: 929 GIPMSISAAVGFIALSGVAVLNGLVMISFIRSLREEGRTLDAAIHEGAMTRLRPVLMTAL 988 Query: 446 AMIIGMIPMALGLGEGAEQNAPLGRAVIGGLIFATVSTLFFVPVVFAGVHQRLARRKEAR 505 +G +PMAL G GAE PL VIGG++ +T TL +P+++ H + A Sbjct: 989 VASLGFVPMALATGTGAEVQRPLATVVIGGILSSTALTLLVLPLLYKLAHDKDEEDNPA- 1047 Query: 506 RQDGRGPADQPP 517 GP +PP Sbjct: 1048 -----GPPSRPP 1054 >gi|300311798|ref|YP_003775890.1| cation/multidrug efflux pump protein [Herbaspirillum seropedicae SmR1] Length = 1051 Score = 96.7 bits (239), Expect = 4e-21, Method: Compositional matrix adjust. Identities = 94/373 (25%), Positives = 158/373 (42%), Gaps = 44/373 (11%) Query: 1 VTIENIERHMHMGKPLHDAIIVGAGEIGVPAFVSTVCICIVFVPMFFLSGVARFLFVPLA 60 V +ENI R++ G +A + GAGEIG +V + VF+P+ + G+ LF A Sbjct: 417 VMLENIYRYVEEGMTPLEAALKGAGEIGFTIISISVSLVAVFIPLLLMGGIVGRLFREFA 476 Query: 61 EAVVFAMAASYILSRTLVPTLVMLLMGGVHGAEGGNSGGKTSLLQRLYRGFDSRFEHVRR 120 V +A S I+S TL P L + HG G +LY+ F+ F+ + Sbjct: 477 VTVTLTIAVSVIISLTLTPMLCSRFLKNEHGQRHG----------KLYQWFERCFDAMLA 526 Query: 121 AYTLVLSSLLSHRRRFALAFLGFCVLSCLLYPFLGRDFFPNVDGGQIRLHMRMPTGTRIE 180 Y L ++ H+ ++F+ L+ +L+ F+ + FFP D G I + Sbjct: 527 GYKRGLDVVMKHQFITLMSFIATLALTAVLFIFIPKGFFPQQDNGVI-----------VG 575 Query: 181 ETARAADQVEAAIREMI--PAHELETILDNIGVPNSGINLSYSNAGTIGTLDGEILMSL- 237 A D AA+ E + A + D IG S AG+ G+ ++L Sbjct: 576 FAESAQDTSSAAMHERLLKVAEVVRQDPDVIGFAMS--------AGSTTFNTGQFFIALK 627 Query: 238 -HEDHRPT--EQLVSMLRAELPKRFPGIEFFFQPADIVTQILNFG---LPAAIDVQFTGP 291 + R T +++++ LR ++ R G+ F Q +Q +N G T Sbjct: 628 PKDQGRTTNADEIIARLRPKV-ARIEGVNLFMQ----ASQDINVGGRLSRTQYQYTITDT 682 Query: 292 NLQQNAELAAQLTKQIRQIPGAVDAHVHQRLDGPALDMQMDRTRLQQFGLSAANVGQNVL 351 +L + A ++ ++ RQ+P D Q+ A + +DR R FG+S + + + Sbjct: 683 DLDELNVWAPRMLERFRQLPQLTDVASDQQNQAAAAVITIDRARASSFGISPSLIDATIY 742 Query: 352 IALSGSSQTTPAF 364 A+ G Q F Sbjct: 743 DAI-GQRQVAQYF 754 Score = 72.8 bits (177), Expect = 6e-14, Method: Compositional matrix adjust. Identities = 48/125 (38%), Positives = 72/125 (57%), Gaps = 4/125 (3%) Query: 386 GTTLSVPALTGAIMTMGVATANSILIVSFA-RQRREEGATPLAAALESGATRIRPVLMTA 444 G LSV AL G I+ +G+ N I+++ FA R +G P A ++ R RP++MT Sbjct: 915 GYDLSVIALIGIILLIGIVKKNGIMMIDFALTAERSQGMKPEQAIYQACLLRFRPIMMTT 974 Query: 445 LAMIIGMIPMALGLGEGAEQNAPLGRAVIGGLIFATVSTLFFVPVVFAGV---HQRLARR 501 + ++ +P+ LG G G+E PLG A++GGLI + TLF PVV+ + H R+ Sbjct: 975 MCALLSGLPLMLGHGAGSELRRPLGYAMVGGLILSQALTLFTTPVVYLYLDRAHYWYMRK 1034 Query: 502 KEARR 506 KEAR+ Sbjct: 1035 KEARQ 1039 >gi|300310690|ref|YP_003774782.1| cation/multidrug efflux pump protein [Herbaspirillum seropedicae SmR1] Length = 1049 Score = 93.2 bits (230), Expect = 4e-20, Method: Compositional matrix adjust. Identities = 52/121 (42%), Positives = 72/121 (59%), Gaps = 10/121 (8%) Query: 396 GAIMTMGVATANSILIVSFARQRREEGATPLAAALESGATRIRPVLMTALAMIIGMIPMA 455 G + T+G+A N+ILIV FA+ E G + + A +E+ R+RP+LMT+LA I+G++PM Sbjct: 929 GLLTTIGLACKNAILIVEFAKDLYEHGKSLVEATMEAVRMRLRPILMTSLAFILGVLPMV 988 Query: 456 LGLGEGAEQNAPLGRAVIGGLIFATVSTLFFVPVVFAGVHQRLARRKEARRQDGRGPADQ 515 LG G GA LG AVIGG++ TV +FFVP+ F + GR PAD Sbjct: 989 LGSGAGAGAQHALGTAVIGGMLSGTVLAVFFVPLFFVIIMTLF----------GRKPADA 1038 Query: 516 P 516 P Sbjct: 1039 P 1039 >gi|300313244|ref|YP_003777336.1| multidrug efflux system transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 1033 Score = 91.7 bits (226), Expect = 1e-19, Method: Compositional matrix adjust. Identities = 49/109 (44%), Positives = 69/109 (63%), Gaps = 3/109 (2%) Query: 396 GAIMTMGVATANSILIVSFARQRREEGATPLAAALESGATRIRPVLMTALAMIIGMIPMA 455 G I +G++ N+ILIV FARQ R EG + AA+++ R+RP+LMT+LA +G++P+ Sbjct: 923 GMITVIGLSAKNAILIVEFARQLRSEGMGLVEAAIKASVLRLRPILMTSLAFALGVVPLM 982 Query: 456 LGLGEGAEQNAPLGRAVIGGLIFATVSTLFFVPVVFA---GVHQRLARR 501 L G AE +G V GG++ ATV + FVPV F G QRLAR+ Sbjct: 983 LASGASAETQRAIGTGVFGGMVSATVLAVLFVPVFFVLVLGCAQRLARK 1031 Score = 58.2 bits (139), Expect = 2e-09, Method: Compositional matrix adjust. Identities = 50/195 (25%), Positives = 76/195 (38%), Gaps = 9/195 (4%) Query: 1 VTIENIERHM-HMGKPLHDAIIVGAGEIGVPAFVSTVCICIVFVPMFFLSGVARFLFVPL 59 V +EN+ER M H G +A I GEI T+ + VF+PM G ++ Sbjct: 411 VVVENVERIMAHEGLGPREATIKAMGEITGAIIGITLVLTAVFIPMALAGGSVGVIYRQF 470 Query: 60 AEAVVFAMAASYILSRTLVPTLVMLLMGGVHGAEGGNSGGKTSLLQRLYRGFDSRFEHVR 119 + A+ ++ S L+ +L P L L+ V G + F+ F + Sbjct: 471 SVAMAVSILFSAFLALSLTPALCATLLRPVPAGHHDKRG--------FFGAFNRAFNRLT 522 Query: 120 RAYTLVLSSLLSHRRRFALAFLGFCVLSCLLYPFLGRDFFPNVDGGQIRLHMRMPTGTRI 179 Y L L++ R L + L + L F P D G ++P Sbjct: 523 ARYESALGRLIARGLRMMLVYAALSGLLVVGMQALPSAFLPEEDQGYFMTSFQLPADATT 582 Query: 180 EETARAADQVEAAIR 194 E TARA DQ E ++ Sbjct: 583 ERTARAVDQFERYLK 597 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.324 0.139 0.404 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,589,602 Number of extensions: 68529 Number of successful extensions: 382 Number of sequences better than 1.0e-08: 11 Number of HSP's gapped: 366 Number of HSP's successfully gapped: 20 Length of query: 522 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 432 Effective length of database: 1,193,720 Effective search space: 515687040 Effective search space used: 515687040 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.5 bits) S2: 133 (55.8 bits)