BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0004.0156.p (470 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313898|ref|YP_003777990.1| outer membrane drug efflux lipo... 660 0.0 gi|300311796|ref|YP_003775888.1| outer membrane channel lipoprot... 286 2e-78 gi|300309656|ref|YP_003773748.1| outer membrane drug efflux prot... 277 1e-75 gi|300310854|ref|YP_003774946.1| NodT family protein [Herbaspiri... 271 1e-73 gi|300309818|ref|YP_003773910.1| drug efflux lipoprotein [Herbas... 216 3e-57 >gi|300313898|ref|YP_003777990.1| outer membrane drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 486 Score = 660 bits (1704), Expect = 0.0, Method: Compositional matrix adjust. Identities = 333/452 (73%), Positives = 388/452 (85%), Gaps = 1/452 (0%) Query: 12 GCAVGPDYRKPDVDMPVTWQLDAPWRQGTPADEQAKGPWWERFGDAKLNALQQQAIDGNQ 71 GCA GPDY +P VDMP W +APWR PAD +GPWWERFGDA+LNALQQQA+ GNQ Sbjct: 18 GCASGPDYTRPQVDMPAAWTSEAPWRSMQPADATPRGPWWERFGDAQLNALQQQALAGNQ 77 Query: 72 TLAIAAARLTQARASANVASAGMYPQVGVSTRTSRLRIAGNRPLTNYNSPNFATVQNDFA 131 TLAIA ARL QARA NV A PQV ++ R +R RI+ NRPLTNYNSPNF+TVQNDFA Sbjct: 78 TLAIATARLAQARAQLNVTGAAQTPQVNLNARAARSRISANRPLTNYNSPNFSTVQNDFA 137 Query: 132 LGLNVNYEVDLAGRVQRNVXARASSQQSAA-DFENTRLLLSADLAGNYFNLRELDVELDV 190 LG +V+YEVD GRVQR+V A ++ + +A D ENTRLLL+A+LA NYFNLRELDVELDV Sbjct: 138 LGASVSYEVDFFGRVQRSVEAAGAAAEQSAADLENTRLLLTAELATNYFNLRELDVELDV 197 Query: 191 LSRSIALQRKALELATSRHDLGATSGLDVAQQQALLDTTLTQVDVLRKSRAQYQHAIATL 250 + R+IALQR+ALELAT+RHDLGATSGLD+AQQQALLD+TLTQ+D+L + RAQY+HAIATL Sbjct: 198 VRRAIALQRQALELATARHDLGATSGLDMAQQQALLDSTLTQIDLLGRQRAQYEHAIATL 257 Query: 251 VGTPAPLFSLAPELVPMTPPAVPLGVPSDLLERRPDIASAERAMAAANAQIGVAKAAFYP 310 GTPAP FS+ P L P+TPPA+P+GVPSD+LERRPDIA+AERAMAAANAQIGVA AA+YP Sbjct: 258 TGTPAPAFSITPSLTPITPPAIPVGVPSDVLERRPDIAAAERAMAAANAQIGVASAAYYP 317 Query: 311 SIMLAPSYGVDSRNIDTLFNAPSVVWSLGVSLAQTIFDGGRVSANVDFAKGGYAASVASY 370 S ML PSYGVDSRN TLFNAPS++WSLGVS +Q++FDGGR+ A VDF++ GY A+VASY Sbjct: 318 SFMLQPSYGVDSRNWATLFNAPSLLWSLGVSASQSLFDGGRLRAGVDFSQAGYEATVASY 377 Query: 371 RRVVLTAMQEVEDGITGVAALDRAYTQAQAAIVSARRVLDLANSRYEGGVSTYLDVISAQ 430 RR VLTAMQEVEDGITG+AAL+RAY Q+Q AI SARRVL++A+SRYEGG + YLDVI+AQ Sbjct: 378 RRTVLTAMQEVEDGITGLAALERAYAQSQLAIASARRVLEIASSRYEGGATPYLDVITAQ 437 Query: 431 QSLLNSERQAAQLLGQRMLTSVFLIKALGGDW 462 QSLLNSERQAAQL+GQR+L SVFLIKALGGDW Sbjct: 438 QSLLNSERQAAQLMGQRLLVSVFLIKALGGDW 469 >gi|300311796|ref|YP_003775888.1| outer membrane channel lipoprotein [Herbaspirillum seropedicae SmR1] Length = 510 Score = 286 bits (732), Expect = 2e-78, Method: Compositional matrix adjust. Identities = 198/479 (41%), Positives = 283/479 (59%), Gaps = 27/479 (5%) Query: 1 VTAVAVAATLAGCAVGPDYRKPDVDMPVTWQLDAPWRQGTPADEQAKGPWWERFGDAKLN 60 +TA+ +AA L GCAVGPDY +P +D+P ++ PW+ P PWWE +GD LN Sbjct: 35 MTALVLAALLGGCAVGPDYARPALDLPQAYKETGPWKLAAPRQIDDHQPWWEAYGDPVLN 94 Query: 61 ALQQQAIDGNQTLAIAAARLTQARASANVASAGMYPQVGVSTRTSRLRIAGNRPLTNYNS 120 AL QA NQT+ AAA+ QA+A+A VA A ++P +G SR + N+ Sbjct: 95 ALMDQANAANQTIQQAAAQYRQAQATAEVARASLWPTIGAQAGASRAQT---------NT 145 Query: 121 PNFATVQNDFALGLNVNYEVDLAGRVQRNVXA-RASSQQSAADFENTRLLLSADLAGNYF 179 + N +++GLN ++E DL GR++R A A++Q SAA RL + A LA +Y Sbjct: 146 NGVQRLGNSYSVGLNASWEADLWGRIRRGAEAGEANAQASAASLAAARLSIQATLAQDYL 205 Query: 180 NLRELDVELDVLSRSIALQRKALELATSRHDLGATSGLDVAQQQALLDTTLTQVDVLRKS 239 LR D++ D+ R++A ++L+L+T +++ G DVAQ + L Q+ L + Sbjct: 206 QLRVTDLQKDLYRRTVAAYTRSLQLSTHQYEAGTALRSDVAQAETQLRAAQAQLIDLDDT 265 Query: 240 RAQYQHAIATLVGTPAPLFSLAPELVPM----------------TPPAVPLGVPSDLLER 283 R Q +HAIA L+G FSL P L P + P +P+G+PS+LLER Sbjct: 266 RNQLEHAIAMLIGKAPAQFSL-PALPPAAASDSAIDANAARLQGSLPQIPVGLPSELLER 324 Query: 284 RPDIASAERAMAAANAQIGVAKAAFYPSIMLAPSYGVDSRNIDTLFNAPSVVWSLGVSLA 343 RPDIA+AER AAANA IGVA+AA++P++ L+ S G +S LFN PS VWSLG +LA Sbjct: 325 RPDIANAERLAAAANANIGVARAAYFPTLTLSASGGYNSLAFANLFNTPSRVWSLGSALA 384 Query: 344 QTIFDGGRVSANVDFAKGGYAASVASYRRVVLTAMQEVEDGITGVAALDRAYTQAQAAIV 403 +++FDGG SA D A Y A+VA Y++ VL +QEVED ++ + LD+ T A+ Sbjct: 385 ESLFDGGARSARNDAAVAAYDAAVAQYKQTVLGGLQEVEDKLSTLRVLDQESTVQAQAVQ 444 Query: 404 SARRVLDLANSRYEGGVSTYLDVISAQQSLLNSERQAAQLLGQRMLTSVFLIKALGGDW 462 SA+ LA +YE G TYL V++ Q + L ++R A LLG++++ SV LIKA GG W Sbjct: 445 SAQLAERLAMRQYEAGTVTYLSVVTTQAASLTNQRNAVTLLGRQLVASVALIKATGGGW 503 >gi|300309656|ref|YP_003773748.1| outer membrane drug efflux protein [Herbaspirillum seropedicae SmR1] Length = 504 Score = 277 bits (709), Expect = 1e-75, Method: Compositional matrix adjust. Identities = 191/476 (40%), Positives = 266/476 (55%), Gaps = 37/476 (7%) Query: 12 GCAVGPDYRKPDVDMPVTWQLDAP----------WRQGTPADEQAKGPWWERFGDAKLNA 61 GC+V P Y +P VD P ++ AP W+ PA++ A+G WW+ FGD KLNA Sbjct: 30 GCSVAPTYERPSVDTPAAFKEAAPATQTGENGSQWKTAQPAEDIARGEWWKIFGDEKLNA 89 Query: 62 LQQQAIDGNQTLAIAAARLTQARASANVASAGMYPQVGVSTRTSRLRI----------AG 111 L+ A NQ L AAARL QARA A AG +PQV +R R A Sbjct: 90 LEDTAQQANQDLKAAAARLGQARALTRDARAGYFPQVDAGVGATRQRPSPASQGLPADAD 149 Query: 112 NRPLTNYNSPNFATVQNDFALGLNVNYEVDLAGRVQRNV-XARASSQQSAADFENTRLLL 170 RP T Y + +NV+YEVDL GRV V A A +Q+S A F + L L Sbjct: 150 TRPNTLYRAQ------------VNVSYEVDLFGRVASTVDAATADAQRSEALFHSVLLAL 197 Query: 171 SADLAGNYFNLRELDVELDVLSRSIALQRKALELATSRHDLGATSGLDVAQQQALLDTTL 230 AD+A YF +RELD + ++ L++++L L R D G S +DVA+ +A L + Sbjct: 198 QADVAQQYFLVRELDAAAALYRGTVDLRQESLRLVQRRFDEGDISEVDVARARAELASAQ 257 Query: 231 TQVDVLRKSRAQYQHAIATLVGTPAPLFSLAPELVPMTPPAVPLGVPSDLLERRPDIASA 290 ++ + + RA +HA+A L+G FSL P+ + VP G+PS LLERRPDIA+A Sbjct: 258 SEALGIARQRATAEHALAILLGRTPAEFSLPPQPLTRLAVTVPPGLPSALLERRPDIAAA 317 Query: 291 ERAMAAANAQIGVAKAAFYPSIMLAPSYGVDSRNIDTLFNAPSVVWSL----GVSLAQTI 346 ERAMAAANA++GVAK+AF+PS+ L G +S + LFN S + L G +L+ + Sbjct: 318 ERAMAAANARVGVAKSAFFPSLNLTGGLGYESAQLGDLFNWSSRTFLLGPLVGTALSMPL 377 Query: 347 FDGGRVSANVDFAKGGYAASVASYRRVVLTAMQEVEDGITGVAALDRAYTQAQAAIVSAR 406 FDGGR A VD A+ Y VASYR VL A +EVED + + LD+ A+ ++ Sbjct: 378 FDGGRRQAGVDRARASYEEQVASYRGTVLNAFREVEDNLANLRILDQQNRAQDEAVAASA 437 Query: 407 RVLDLANSRYEGGVSTYLDVISAQQSLLNSERQAAQLLGQRMLTSVFLIKALGGDW 462 R L++++Y G +Y VI A +++L +R + L G+R ++V LI+ALGG W Sbjct: 438 RAAKLSHTQYREGAVSYFSVIDADRTVLQQQRASVTLDGERARSTVNLIRALGGGW 493 >gi|300310854|ref|YP_003774946.1| NodT family protein [Herbaspirillum seropedicae SmR1] Length = 507 Score = 271 bits (692), Expect = 1e-73, Method: Compositional matrix adjust. Identities = 177/461 (38%), Positives = 257/461 (55%), Gaps = 8/461 (1%) Query: 10 LAGCAVGPDYRKPDVDM-PVTWQLDAPWRQGTPADEQAKGPWWERFGDAKLNALQQQAID 68 LA C+VGPDY +P +D P ++ W+ P D A G WWE FGD +LNAL Q Sbjct: 22 LAACSVGPDYVRPQMDTGPAIFKEGQNWKPAQPRDLVADGKWWEMFGDPQLNALVAQVDI 81 Query: 69 GNQTLAIAAARLTQARASANVASAGMYPQVGVSTRTSRLRIAGNRPLTNYNSPNFAT--V 126 NQ LA+A A A A + + P + S SR ++ + + V Sbjct: 82 SNQNLALAEANFRSAVALVQSSRSAYSPTLSASAGASRGSSGSAGASSSNAASGSSAGSV 141 Query: 127 QNDFALGLNVNYEVDLAGRVQRNVXARASSQQSAADFENTRLLL-SADLAGNYFNLRELD 185 +N + L +E D+ G+V+R+V + +S Q++A L A +A NY LR LD Sbjct: 142 RNSYTFSLTAAWEADIWGKVRRDVESSQASAQASAADLAAARLSAQAQVAQNYLQLRVLD 201 Query: 186 VELDVLSRSIALQRKALELATSRHDLGATSGLDVAQQQALLDTTLTQVDVLRK--SRAQY 243 + +L ++ ++L++A +++ G + DV Q Q L + Q L +RAQ Sbjct: 202 AQQQLLEETVQAYERSLQVAQNQYAAGMVAKSDVIQAQTQLKSA--QASALDNGVTRAQV 259 Query: 244 QHAIATLVGTPAPLFSLAPELVPMTPPAVPLGVPSDLLERRPDIASAERAMAAANAQIGV 303 +HAIA LVG F+L + P VP GVPS LLERRPD+A+AER +A+ANA+IGV Sbjct: 260 EHAIALLVGKTPAQFALPRAPLAAVAPPVPQGVPSMLLERRPDVAAAERRVASANAKIGV 319 Query: 304 AKAAFYPSIMLAPSYGVDSRNIDTLFNAPSVVWSLGVSLAQTIFDGGRVSANVDFAKGGY 363 AKAA++PS+ L+ S G S F P VWSLG LAQTIFDGG +A D A Y Sbjct: 320 AKAAYFPSLTLSASGGYQSSTFADWFTLPGRVWSLGPQLAQTIFDGGARAAATDQAIATY 379 Query: 364 AASVASYRRVVLTAMQEVEDGITGVAALDRAYTQAQAAIVSARRVLDLANSRYEGGVSTY 423 +VA+Y++ VLTA QEVED + + L++ A+ +AR+ + L N++Y+ G +Y Sbjct: 380 DGTVATYKQTVLTAFQEVEDNLAALRILEQEAAVQGEALQAARQAVTLVNNQYKAGTVSY 439 Query: 424 LDVISAQQSLLNSERQAAQLLGQRMLTSVFLIKALGGDWPD 464 ++VI+AQ + L++E + +L +RM SV L KALGG W + Sbjct: 440 VNVITAQATALSAELSSLNILNRRMAASVLLAKALGGGWDE 480 >gi|300309818|ref|YP_003773910.1| drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 483 Score = 216 bits (550), Expect = 3e-57, Method: Compositional matrix adjust. Identities = 154/470 (32%), Positives = 246/470 (52%), Gaps = 25/470 (5%) Query: 1 VTAVAVAATLAGCAVGPDYRKPDVDMPVTWQLDAPWRQGTPADEQAKGPWWERF-GDAKL 59 A+ +A L GC++ P Y +P + ++ DA R A+ QA W +F D +L Sbjct: 16 CAALVMAGVLGGCSLAPTYERPQAPVESSYPADAAGR----AELQAVNLGWRQFFPDQRL 71 Query: 60 NALQQQAIDGNQTLAIAAARLTQARASANVASAGMYPQVGVSTRTSRLRIAGNRPLTNYN 119 L A++ N+ L A + +ARA +A A + P + +S +R R A ++ LT Sbjct: 72 QQLIAAALENNRDLRTAVLNIEEARAQYQIARADLLPNLNLSGNAARTRTAASQSLTGQ- 130 Query: 120 SPNFATVQNDFALGLNV-NYEVDLAGRVQRNVX--ARASSQQSAADFENTRLLLSADLAG 176 T+ N + +GL V YE+D GRV RN+ A AS + ++ ++ L A +A Sbjct: 131 ----PTISNSYQVGLAVPAYELDFFGRV-RNLKDAALASYLSTEEAQKSAQISLVAQVAQ 185 Query: 177 NYFNLRELDVELDVLSRSIALQRKALELATSRHDLGATSGLDVAQQQALLDTTLTQVDVL 236 Y R +L + ++ ++ L+LA R D+GA++ LD Q L+++ L Sbjct: 186 AYLAERSYAEQLKLADETLKSRQSNLDLAQKRLDVGASTALDFRLTQTLVESARVAKTQL 245 Query: 237 RKSRAQYQHAIATLVGT------PAPLFSLAPELVPMTPPAVPLGVPSDLLERRPDIASA 290 + RAQ ++A+ LVG PA L S + +P G+PSDLL RPDI SA Sbjct: 246 ERQRAQAENALTLLVGKKIGELPPAQLLSDEKIVTD-----IPAGLPSDLLTNRPDIRSA 300 Query: 291 ERAMAAANAQIGVAKAAFYPSIMLAPSYGVDSRNIDTLFNAPSVVWSLGVSLAQTIFDGG 350 E+++ AANA IG A+AAF+P I L ++G S + LF + S WS G SL+ IFD G Sbjct: 301 EQSLLAANANIGAARAAFFPRISLTGNFGSASSTLGGLFESGSGAWSFGPSLSLPIFDFG 360 Query: 351 RVSANVDFAKGGYAASVASYRRVVLTAMQEVEDGITGVAALDRAYTQAQAAIVSARRVLD 410 R SAN+D A+ +V +Y + + TA +EV D + +LD +A + + + L Sbjct: 361 RNSANLDLAEVRKNKAVVTYEKAIQTAFREVADALVARGSLDEQVKAQEAFLAAQQERLK 420 Query: 411 LANSRYEGGVSTYLDVISAQQSLLNSERQAAQLLGQRMLTSVFLIKALGG 460 L + R++ G+++ LD + AQ+ L ++++ Q R+ ++ L KALGG Sbjct: 421 LTDLRFKNGIASSLDQLDAQRDLFSAQQALIQARQLRLNNAIDLYKALGG 470 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.317 0.129 0.365 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,258,772 Number of extensions: 47367 Number of successful extensions: 274 Number of sequences better than 1.0e-08: 18 Number of HSP's gapped: 222 Number of HSP's successfully gapped: 18 Length of query: 470 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 381 Effective length of database: 1,198,455 Effective search space: 456611355 Effective search space used: 456611355 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)