BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0004.0155.p (480 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312566|ref|YP_003776658.1| multidrug resistance protein pr... 818 0.0 gi|300312017|ref|YP_003776109.1| multidrug resistance B (translo... 407 e-115 gi|300312486|ref|YP_003776578.1| major facilitator superfamily p... 206 5e-54 gi|300312683|ref|YP_003776775.1| major facilitator superfamily p... 131 2e-31 gi|300310856|ref|YP_003774948.1| major facilitator superfamily p... 130 2e-31 >gi|300312566|ref|YP_003776658.1| multidrug resistance protein protein [Herbaspirillum seropedicae SmR1] Length = 525 Score = 818 bits (2114), Expect = 0.0, Method: Compositional matrix adjust. Identities = 397/480 (82%), Positives = 440/480 (91%) Query: 1 VLDTTIANVSVSTISGALGASSSQGTYVITSYAVAEAITVPLTGWLANRFGTVRVFITSM 60 VLDTTIANVSVSTI+GALGAS+SQGTYVITSYAVAEAITVPLTGWLANRFGTVRVFI SM Sbjct: 46 VLDTTIANVSVSTIAGALGASTSQGTYVITSYAVAEAITVPLTGWLANRFGTVRVFILSM 105 Query: 61 ILFGVFSALCGMANSLGMLIAFRVLQGLAGGPLMPLSQTLLLRIFPKEKAAAAVGLWAMT 120 ++FG+FSALCGMANSLGMLIAFRVLQGLAGGPLMPLSQTLLLRIFPKEKA AAVGLWAMT Sbjct: 106 VMFGLFSALCGMANSLGMLIAFRVLQGLAGGPLMPLSQTLLLRIFPKEKAPAAVGLWAMT 165 Query: 121 TLIAPIAGPILGGVLCDQYSWPYIFFINVPVALICGYMGWKMLKRYESPLLKLPIDKVGL 180 TL+APIAGPILGGVLCDQYSWPYIF+INVPVALICGY+GW+MLKRYE+ + KLPID+VGL Sbjct: 166 TLVAPIAGPILGGVLCDQYSWPYIFWINVPVALICGYIGWRMLKRYETKMSKLPIDRVGL 225 Query: 181 ILMVIWVAALQLMLDEGKEKDWFASTEIVVLALIAAAGFIAFLIWELTERNPIVDLRVFR 240 +LMV+WVAALQLMLDEGKEKDWFASTEIV+LA+ A FIAFLIWELTERNPIVDLRVFR Sbjct: 226 VLMVVWVAALQLMLDEGKEKDWFASTEIVLLAVTAVVFFIAFLIWELTERNPIVDLRVFR 285 Query: 241 HRGYTASVTTICLAFGAFFGATVLTPLWLQGYMGYTATHSGETTALTGILAVLTAPFVAK 300 HRG+TASVTTICLAFGAFFG+ VLTPLWLQGYMGYTAT SG +ALTGILAV+TAP VAK Sbjct: 286 HRGFTASVTTICLAFGAFFGSVVLTPLWLQGYMGYTATWSGCISALTGILAVMTAPMVAK 345 Query: 301 LSTRVDPRKLVFIGVMWLGAVTLFRSFNTTDMSNFQIGWPLLMLGIGLPLFFVPLTGLAL 360 +ST +D RKLV IGV WLG +T R+FNTTDMS FQIGWPL + GIGLPLFFVPLTGLAL Sbjct: 346 MSTTIDARKLVCIGVTWLGFITFLRAFNTTDMSFFQIGWPLFLQGIGLPLFFVPLTGLAL 405 Query: 361 SSVEESETASAAGLMSFCRTMSGAIATSLVNTSWENKSIYYHAELAGMVDQFGDVSNTLA 420 SSV E ETASAAGLMSFCRTM+GAIATS+VNTSWEN + Y+HAEL+G+VD+ GD +T+ Sbjct: 406 SSVNEEETASAAGLMSFCRTMAGAIATSIVNTSWENDATYFHAELSGLVDRGGDAIHTMM 465 Query: 421 QAGMSLDQARANMEQMLQSQSIMLSTNHIFMMAAISFGLAAAAVWFAPKPKRVADTSAAH 480 +GMS +QA A++ Q +Q+Q++MLSTNHIFMMAA+SF LAA AVW APKP RVADTSAAH Sbjct: 466 ASGMSAEQATASLAQTVQNQAVMLSTNHIFMMAAVSFSLAALAVWLAPKPTRVADTSAAH 525 >gi|300312017|ref|YP_003776109.1| multidrug resistance B (translocase) transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 524 Score = 407 bits (1047), Expect = e-115, Method: Compositional matrix adjust. Identities = 213/468 (45%), Positives = 297/468 (63%), Gaps = 17/468 (3%) Query: 1 VLDTTIANVSVSTISGALGASSSQGTYVITSYAVAEAITVPLTGWLANRFGTVRVFITSM 60 VLD++IANV++ TISG LG S +GT+VITS+A A AI++PLTGWL R G VR+F+TS+ Sbjct: 37 VLDSSIANVAIPTISGNLGVSVDEGTWVITSFAAANAISIPLTGWLTQRLGAVRLFVTSV 96 Query: 61 ILFGVFSALCGMANSLGMLIAFRVLQGLAGGPLMPLSQTLLLRIFPKEKAAAAVGLWAMT 120 +LF + S LCG+A +L +L+A RVLQGL GP++PLSQ+LLL +PK K++ A+ LW MT Sbjct: 97 LLFVLASWLCGLAPTLPILLAARVLQGLVAGPMVPLSQSLLLGAYPKSKSSFALALWGMT 156 Query: 121 TLIAPIAGPILGGVLCDQYSWPYIFFINVPVALICGYMGWKMLKRYESPLLKLPIDKVGL 180 ++AP+AGP LGG + D Y+WP+IF+IN+PV LI + + + E+P KLPIDK+GL Sbjct: 157 AVVAPVAGPALGGWITDSYTWPWIFYINIPVGLIAAGVTLAIYRSRETPTHKLPIDKIGL 216 Query: 181 ILMVIWVAALQLMLDEGKEKDWFASTEIVVLALIAAAGFIAFLIWELTERNPIVDLRVFR 240 L+V WVAA Q+MLD+GK+ DWFAS IV L +IA F F++WEL +P+VDLR+F Sbjct: 217 ALLVTWVAAFQIMLDKGKDLDWFASPTIVTLGIIALIAFAYFVVWELNNDHPVVDLRLFG 276 Query: 241 HRGYTASVTTICLAFGAFFGATVLTPLWLQGYMGYTATHSGETTALTGILAVLTAPFVAK 300 R + I + +G FFG V+ P WLQ Y+GY + SG +TA GI AV+ P + + Sbjct: 277 RRNFAGGTIAISIGYGVFFGNLVILPQWLQQYLGYRSIDSGLSTAPIGIFAVILMPLIGR 336 Query: 301 LSTRVDPRKLV---FIG---VMWLGAVTLFRSFNTTDMSNFQIGWPLLMLGIGLPLFFVP 354 VD RK+ FIG V WL RS + + P L+ GI +FFVP Sbjct: 337 FLPLVDARKVATASFIGFAIVFWL------RSRYNLQVDQMTVILPTLLQGIPTAMFFVP 390 Query: 355 LTGLALSSVEESETASAAGLMSFCRTMSGAIATSLVNTSWENKSIYYHAELAGMVDQFGD 414 LT L LS + +AAGL SF R GA+ TS+ T+W N+SI +HA+L + Sbjct: 391 LTVLLLSGLPPERIPAAAGLSSFVRVFCGAVGTSISTTAWNNRSIMHHAQLTEHATPYSP 450 Query: 415 -----VSNTLAQAGMSLDQARANMEQMLQSQSIMLSTNHIFMMAAISF 457 V+N + G++ +Q +E+ + Q+ ML N IF ++A+ F Sbjct: 451 WLQETVNNLSSTLGLNAEQTAGMLERTITFQAAMLGINDIFYVSAVIF 498 >gi|300312486|ref|YP_003776578.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 514 Score = 206 bits (523), Expect = 5e-54, Method: Compositional matrix adjust. Identities = 151/496 (30%), Positives = 253/496 (51%), Gaps = 29/496 (5%) Query: 2 LDTTIANVSVSTISGALGASSSQGTYVITSYAVAEAITVPLTGWLANRFGTVRVFITSMI 61 LD +I N S+ TI G +GAS ++GT++ T+Y VAE + +P++ WL G + +T+ Sbjct: 31 LDISIVNSSLPTIQGEIGASGTEGTWIATAYLVAEIVIIPMSAWLERLLGLRVLLLTAAS 90 Query: 62 LFGVFSALCGMANSLGMLIAFRVLQGLAGGPLMPLSQTLLLRIFPKEKAAAAVGLWAMTT 121 LF +FS LCG++ +L M+I RV QG GG L+P + T++ P+ + ++ T Sbjct: 91 LFTLFSVLCGLSTTLPMMIVGRVGQGFTGGALIPTAMTIIATRLPRSQQPVGNAMFGATA 150 Query: 122 LIAPIAGPILGGVLCDQYSWPYIFFINVPV--ALICGYMGWKMLKRYESPLLK--LPIDK 177 ++ P+ GP++GG L + SW Y FFIN+PV ALI + + YE P + D Sbjct: 151 ILGPVLGPVMGGWLTENVSWHYAFFINLPVGAALITLLL---VAIPYERPKFEQLAEADW 207 Query: 178 VGLILMVIWVAALQLMLDEGKEKDWFASTEIVVLALIAAAGFIAFLIWELTERNPIVDLR 237 G+ + + + L ++L+EG+ + WF+S +I LA + GFI I ++ R+P++ LR Sbjct: 208 FGIAGLALGLGGLTVVLEEGEREQWFSSPDIRWLAATSVLGFILLAIGQVRARHPVIQLR 267 Query: 238 VFRHRGYTASVTTICLAFGAFFGATVLTPLWLQGYMGYTATHSGETTALTGILAVLTAPF 297 + R R + + V + A +G + P +L G GY + SG L+G+ +L PF Sbjct: 268 LLRDRQFGSVVMMAMIVGVALYGTAYVIPQFLSGIAGYNSLQSGWIVLLSGVPMILMMPF 327 Query: 298 VAKLSTRVDPRKLVFIGVMWLGAVTLFRSFNTTDMSNFQIGWPL----LMLGIGLPLFFV 353 L VD R V G + L A +F TD+S G LM G+G L + Sbjct: 328 TPLLMRLVDIRIAVGTGFLLLAA----SAFIETDLSPLSYGGSFVASQLMRGVGTVLAML 383 Query: 354 PLTGLALSSVEESETASAAGLMSFCRTMSGAIATSLVNTSWENKSIYYHAELAGMVDQFG 413 L A+ SV S+ A+GL S R + G++A + + +++ ++ H+ +++ Sbjct: 384 FLNQAAIRSVPPSQAGDASGLYSAARNLGGSLALAAIAVI-QDQRLWLHSR---RLEESL 439 Query: 414 DVSNTLAQAGMSL--------DQARANMEQMLQSQSIMLSTNHIFMMAAISFGLAAAAVW 465 + ++ L Q+ M+ D A M +QSQ++ ++ +F M ++ V Sbjct: 440 NANSDLVQSSMTAQGHLLGGQDVALQLMGATIQSQALTMTYADLFWMLGVAILCVTPLVL 499 Query: 466 F-APKPKRVADTSAAH 480 F P P A A+H Sbjct: 500 FLRPLPTHAAPV-ASH 514 >gi|300312683|ref|YP_003776775.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 498 Score = 131 bits (329), Expect = 2e-31, Method: Compositional matrix adjust. Identities = 109/394 (27%), Positives = 184/394 (46%), Gaps = 29/394 (7%) Query: 2 LDTTIANVSVSTISGALGASSSQGTYVITSYAVAEAITVPLTGWLANRFGTVRVFITSMI 61 LDTTI N ++ ++ +LG V+ +Y + A P +GWLA+RFGT R++ +++ Sbjct: 67 LDTTIVNTALPAMARSLGEPVLNMHPVVVAYTLTMASLTPASGWLADRFGTRRMYFAAIL 126 Query: 62 LFGVFSALCGMANSLGMLIAFRVLQGLAGGPLMPLSQTLLLRIFPKEKAAAAVGLWAMTT 121 LF + S LC MA+SL ML+A RV+QGL G L+P+ + +LR P + AA+ ++ Sbjct: 127 LFSLGSLLCAMASSLPMLLAARVVQGLGGSMLLPVGRLAVLRAVPGAQYIAALASISIAG 186 Query: 122 LIAPIAGPILGGVLCDQYSWPYIFFINVPVALICGYMGWKMLKRYESPLLKLP-IDKVGL 180 + P+ GP+LGG L + SW +IF IN+PV ++ MG + + L P D +G Sbjct: 187 QLGPVVGPVLGGWLVESLSWHWIFLINLPVGVLG--MGAVLYFLPDDSLAGTPRFDLLGC 244 Query: 181 ILMVIWVAALQLMLD---EGKEKDWFASTEIVVLALIAAAGFIAFLIWEL--TERNPIVD 235 +L+ + A L LD + W S + V+A + G+ W P+ Sbjct: 245 VLLSACMVAFSLALDVPFDSHRTGW--SIALFVVAALTVLGY-----WRHASVHPRPLFR 297 Query: 236 LRVFRHRGYTASVTTICLAFGAFFGATVLTPLWLQGYMGYTATHSGETTALTGILAVLTA 295 L +F ++ + + L PL LQ +GY+ H+G I + Sbjct: 298 LGLFGEPNFSIGLAGNLVCRIGSSAVPFLLPLLLQVRLGYSPLHAGMVLLPAAIAGTIAK 357 Query: 296 PFVAKLSTRVDPRKLVFIGVMWLGAVTLFRSFNTTDMSNFQIGWPLLMLGIGLPLF---- 351 P++A L R + + + +GA + + T D WPL + L +F Sbjct: 358 PWIAPLVKRFGYDRFLLVNTAIVGAAIMSFALITPD-------WPLTAAIVQLAIFGASN 410 Query: 352 ---FVPLTGLALSSVEESETASAAGLMSFCRTMS 382 F + + L +++ + S L S + ++ Sbjct: 411 SMQFAAMNSVTLKGLDKRDAGSGNSLFSMVQMLA 444 >gi|300310856|ref|YP_003774948.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 446 Score = 130 bits (328), Expect = 2e-31, Method: Compositional matrix adjust. Identities = 104/415 (25%), Positives = 200/415 (48%), Gaps = 23/415 (5%) Query: 2 LDTTIANVSVSTISGALGASSSQGTYVITSYAVAEAITVPLTGWLANRFGTVRVFITSMI 61 +D+T+ + S+ ++ + +TSY V+ A+ +P++GW+A+RFG++ +F +++ Sbjct: 1 MDSTVISTSLPALALDMNVDPISLKLALTSYLVSLAVFIPISGWMADRFGSLTIFRSAIC 60 Query: 62 LFGVFSALCGMANSLGMLIAFRVLQGLAGGPLMPLSQTLLLRIFPKEKAAAAVGLWAMTT 121 +F + S LCG++++L +A R LQG+ G ++P+ + ++LR K A+ + Sbjct: 61 VFMLGSILCGVSSTLPQFVAARFLQGIGGAMMVPVGRLVILRSVEKVNLVKALSYLTIPA 120 Query: 122 LIAPIAGPILGGVLCDQYSWPYIFFINVPVALICGYMGWKMLKRYESPLLKLPIDKVGLI 181 L P+ GP LGG + + W +IFFIN+P++++ Y+ + + ++ + P+D G + Sbjct: 121 LFGPVLGPPLGGFITTYFHWRWIFFINIPISILGLYLATRFIPDLKAEQVA-PLDLKGFL 179 Query: 182 LMVIWVAALQLMLDEGKEKDWFASTEIVVLALIAAAGFIAFLIWELTERN-PIVDLRVFR 240 L + + L + L S E+ + ++A F+A +W + P++DL + + Sbjct: 180 LSALGCSLLMMGLATAGRH--LVSGEVALTCVLAGLVFLAAYVWHARRASAPLIDLALLK 237 Query: 241 HRGYTASVTTICLAFGAFFGATV-----LTPLWLQGYMGYTATHSGETTALTGILAVLTA 295 A V G+F+ + L PL LQ GYT HSG T + A+ Sbjct: 238 IPTLRAGVFG-----GSFYRVGIGAIPFLLPLMLQLGFGYTPFHSGMLTCASAAGAMFMK 292 Query: 296 PFVAKLSTRVDPRKLVFI----GVMWLGAVTLFRSFNTTDMSNFQIGWPLLMLGIGLPLF 351 A+ R R+++ + GV+ L A+ F T + LL+ G L Sbjct: 293 TIAARFLRRYGFRRVLLVNGALGVLGLWAIATF----TPQTPYLLMVVILLIGGCFRSLQ 348 Query: 352 FVPLTGLALSSVEESETASAAGLMSFCRTMSGAIATSLVNTSWE-NKSIYYHAEL 405 F L ++ + V++ + A + S + +S + L + + + I HA L Sbjct: 349 FTSLNAISYADVDKLRLSQATSMASVAQQLSIGMGVVLAGFALQMSNEIQGHATL 403 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.325 0.136 0.413 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,378,658 Number of extensions: 53634 Number of successful extensions: 344 Number of sequences better than 1.0e-08: 18 Number of HSP's gapped: 328 Number of HSP's successfully gapped: 18 Length of query: 480 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 391 Effective length of database: 1,198,455 Effective search space: 468595905 Effective search space used: 468595905 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 132 (55.5 bits)