BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0004.0149.p (516 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313897|ref|YP_003777989.1| methyl-accepting chemotaxis pro... 666 0.0 gi|300312038|ref|YP_003776130.1| methyl-accepting chemotaxis tra... 615 e-177 gi|300310474|ref|YP_003774566.1| methyl-accepting chemotaxis tra... 584 e-168 gi|300313024|ref|YP_003777116.1| methyl-accepting chemotaxis pro... 568 e-163 gi|300309735|ref|YP_003773827.1| methyl-accepting chemotaxis sen... 544 e-156 >gi|300313897|ref|YP_003777989.1| methyl-accepting chemotaxis protein I [Herbaspirillum seropedicae SmR1] Length = 516 Score = 666 bits (1719), Expect = 0.0, Method: Compositional matrix adjust. Identities = 362/516 (70%), Positives = 428/516 (82%) Query: 1 MRWFYNLKIAKKLVLSFLAVIILTAGLGAFSIVELIKVNRASSDIATNWMPSVKVGLQLQ 60 M WF NLKIAKKL++SF AVI+LT LG F+I EL VN+AS+D+ATNW+P++K +Q Sbjct: 1 MTWFNNLKIAKKLIVSFAAVIVLTVILGLFAIRELKAVNQASTDMATNWLPTIKAAQSIQ 60 Query: 61 ASMSRFRISELQHILATEEKDYDDAEKAMKTRMEIIRKDLASYEQMISEPEEKVLYPQFQ 120 AS+ R RISELQ + A + +DA KA+K+R++I+ K A+YE ISE EEKVLY +F Sbjct: 61 ASLPRMRISELQMVTAATPAEREDALKAIKSRLDILAKQRAAYEAQISEAEEKVLYEKFS 120 Query: 121 QTMQKYLAENKKIMELSRANQKDEARLQFKGESNKLFRQAVEQLGGLVKINDDGTVRSNA 180 ++ YL +K++ +S +++EAR FKG+SNK FR +E + ++K+ND G+ RS+ Sbjct: 121 KSFATYLEIDKELARISAEGKEEEARALFKGDSNKTFRSMIENMDEIIKVNDAGSKRSDD 180 Query: 181 EADHTFNEARNWILGLLIAIVVIAMTLAVWVARIVSVPLNKAVDVAQRVAEGDLGADIRS 240 A+ + A WI+GLL AIVVIA+ LAV VAR VS PLN+AV VA+RVA+GDL DIR+ Sbjct: 181 MAEQVYRSATLWIIGLLAAIVVIALGLAVMVARAVSRPLNEAVAVAERVAQGDLTMDIRA 240 Query: 241 DSRDETGQLMDSLKAMNDSLLRIVGEVRTGTDTIATASTEIASGNLDLSSRTEQQAGSLE 300 S DETG LM SL+AMNDSL +IVGEVRTGTDTI TAS EIA+GNLDLSSRTE+QAGSLE Sbjct: 241 TSTDETGMLMHSLRAMNDSLQQIVGEVRTGTDTITTASQEIATGNLDLSSRTEEQAGSLE 300 Query: 301 ETASAMEELTSTVKANADNARQANQLAVSASEVAQQGGAVVGQVVDTMGSINESSRKIVD 360 ETASAMEELT+TVK NADNARQANQLA SAS++A QGGAVVGQVV TM I ESS +I D Sbjct: 301 ETASAMEELTATVKQNADNARQANQLAASASDIATQGGAVVGQVVQTMEGITESSNRIAD 360 Query: 361 IISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSAAAAKEIKVLIDD 420 IISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSA+AAKEIK LIDD Sbjct: 361 IISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSASAAKEIKQLIDD 420 Query: 421 SVEKVGSGSKLVEQAGVTMSEVVASVRRVTDIVGEISAASTEQSTGIEEVNRAITQMDEV 480 SVEKVG+GS+LVE+AG TMSEVVASVRRVTD+V EISAAS EQSTGIEE+NRAITQMDEV Sbjct: 421 SVEKVGAGSQLVERAGATMSEVVASVRRVTDVVAEISAASNEQSTGIEEINRAITQMDEV 480 Query: 481 TQQNAALVEQAAAAAQSLQEQAGKLAQVVSVFKLRR 516 TQQNAALVE+AAAAAQSLQ+QA +L+QVVSVFKL+R Sbjct: 481 TQQNAALVEEAAAAAQSLQDQAARLSQVVSVFKLQR 516 >gi|300312038|ref|YP_003776130.1| methyl-accepting chemotaxis transmembrane I protein [Herbaspirillum seropedicae SmR1] Length = 579 Score = 615 bits (1587), Expect = e-177, Method: Compositional matrix adjust. Identities = 336/514 (65%), Positives = 409/514 (79%) Query: 1 MRWFYNLKIAKKLVLSFLAVIILTAGLGAFSIVELIKVNRASSDIATNWMPSVKVGLQLQ 60 M+WFY+L+I++KL+ +FL V+ + A LG FSI +L +VN AS++I+TNW+PS++ +LQ Sbjct: 1 MKWFYDLRISRKLITTFLVVVAMVAALGVFSIRQLDQVNSASTEISTNWLPSIRTLARLQ 60 Query: 61 ASMSRFRISELQHILATEEKDYDDAEKAMKTRMEIIRKDLASYEQMISEPEEKVLYPQFQ 120 +MSR R ELQHILA + K+Y++ E+ M+ +M ++ A Y ISEPEEK +YP+ + Sbjct: 61 LAMSRLRSFELQHILAKDAKEYEEVERGMQVQMNNLKTAQAEYVTQISEPEEKAIYPEVE 120 Query: 121 QTMQKYLAENKKIMELSRANQKDEARLQFKGESNKLFRQAVEQLGGLVKINDDGTVRSNA 180 + + +++E+ KI+ LSR + +EAR +GES K +R +++Q+ L +ND G SN Sbjct: 121 KLIATFVSEHDKIIALSRQGKDEEARNLQRGESTKAYRTSLQQIDKLAAVNDKGADISNK 180 Query: 181 EADHTFNEARNWILGLLIAIVVIAMTLAVWVARIVSVPLNKAVDVAQRVAEGDLGADIRS 240 +AD + +AR WI+ + A + +AM LAVWV+R+++ PL +AV VAQRVA GDL I Sbjct: 181 QADAIYAQARLWIIVTVAACIGLAMLLAVWVSRLIARPLEEAVSVAQRVAGGDLTTRIEP 240 Query: 241 DSRDETGQLMDSLKAMNDSLLRIVGEVRTGTDTIATASTEIASGNLDLSSRTEQQAGSLE 300 +DETGQL+ +LK+MNDSL+ IVG+VR GT TI TASTEIASGNLDLSSRTE+QA SLE Sbjct: 241 AGKDETGQLLVALKSMNDSLVGIVGQVRMGTQTITTASTEIASGNLDLSSRTEEQASSLE 300 Query: 301 ETASAMEELTSTVKANADNARQANQLAVSASEVAQQGGAVVGQVVDTMGSINESSRKIVD 360 ETASAMEELTSTVK NADNARQANQLAVSASEVA QGGAVV QVVDTMGSIN SSRKI D Sbjct: 301 ETASAMEELTSTVKQNADNARQANQLAVSASEVASQGGAVVNQVVDTMGSINASSRKIAD 360 Query: 361 IISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSAAAAKEIKVLIDD 420 II VIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSA AAKEIK LI+ Sbjct: 361 IIGVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSAGAAKEIKELIEG 420 Query: 421 SVEKVGSGSKLVEQAGVTMSEVVASVRRVTDIVGEISAASTEQSTGIEEVNRAITQMDEV 480 SV +V GSKLVE+AG+TMSEVV SVRRVTDIVGEIS+AS EQS GIE+VN AI QMDEV Sbjct: 421 SVAQVDMGSKLVERAGITMSEVVQSVRRVTDIVGEISSASQEQSEGIEQVNLAIAQMDEV 480 Query: 481 TQQNAALVEQAAAAAQSLQEQAGKLAQVVSVFKL 514 TQQNAALVE+AAAAAQSLQEQA L Q V FKL Sbjct: 481 TQQNAALVEEAAAAAQSLQEQAETLTQTVGFFKL 514 >gi|300310474|ref|YP_003774566.1| methyl-accepting chemotaxis transducer transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 515 Score = 584 bits (1506), Expect = e-168, Method: Compositional matrix adjust. Identities = 326/514 (63%), Positives = 386/514 (75%) Query: 1 MRWFYNLKIAKKLVLSFLAVIILTAGLGAFSIVELIKVNRASSDIATNWMPSVKVGLQLQ 60 M WFYNL IAKKL LSF VI LT G F+I EL +VN+AS +I + WMP++ +LQ Sbjct: 1 MIWFYNLNIAKKLALSFAVVIALTLLQGLFAIKELKQVNKASGEIVSKWMPTMDAARELQ 60 Query: 61 ASMSRFRISELQHILATEEKDYDDAEKAMKTRMEIIRKDLASYEQMISEPEEKVLYPQFQ 120 S+ R++EL+ + A ++ A KA+K R++ + + A +EQ +SEP+EK Y QF Sbjct: 61 GSLPPLRLNELELVTAVTPQERAAANKAIKDRLDALTRQRAIFEQRMSEPQEKEQYAQFL 120 Query: 121 QTMQKYLAENKKIMELSRANQKDEARLQFKGESNKLFRQAVEQLGGLVKINDDGTVRSNA 180 + + Y+ ++++ E++ Q DE R F G+S KL+R V L +VKIN G+ +++ Sbjct: 121 KNLTAYVKVDEQLAEMAANQQYDETRALFNGDSAKLYRSMVGNLDAIVKINTSGSAQADE 180 Query: 181 EADHTFNEARNWILGLLIAIVVIAMTLAVWVARIVSVPLNKAVDVAQRVAEGDLGADIRS 240 EA F A+ WI+GLL A V+IA LA VA VS PL +AV +AQRVA GDL IR Sbjct: 181 EASTVFRGAQRWIVGLLAATVLIASLLAFLVATNVSRPLKEAVQIAQRVARGDLTLRIRP 240 Query: 241 DSRDETGQLMDSLKAMNDSLLRIVGEVRTGTDTIATASTEIASGNLDLSSRTEQQAGSLE 300 RDETG LM++L+AMN+SL IV EVR GTDTI TAS EIA GNLDLS+RTEQQAGSLE Sbjct: 241 AGRDETGALMEALRAMNESLRDIVSEVRQGTDTITTASHEIARGNLDLSNRTEQQAGSLE 300 Query: 301 ETASAMEELTSTVKANADNARQANQLAVSASEVAQQGGAVVGQVVDTMGSINESSRKIVD 360 ETASAMEELTSTV NADNARQANQLA +AS++A QGG VVGQVV TM I ESSR+I D Sbjct: 301 ETASAMEELTSTVTQNADNARQANQLAATASDIASQGGEVVGQVVQTMEGITESSRRIAD 360 Query: 361 IISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSAAAAKEIKVLIDD 420 IISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSA AAKEIK LIDD Sbjct: 361 IISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSAGAAKEIKELIDD 420 Query: 421 SVEKVGSGSKLVEQAGVTMSEVVASVRRVTDIVGEISAASTEQSTGIEEVNRAITQMDEV 480 SV+KV +GS LVE+AG TM+EVVASVRRVTD+V EISAA+ EQS GI EVN AITQMDE Sbjct: 421 SVQKVSAGSALVERAGSTMTEVVASVRRVTDVVAEISAATQEQSGGINEVNLAITQMDET 480 Query: 481 TQQNAALVEQAAAAAQSLQEQAGKLAQVVSVFKL 514 TQQNAALVEQAAAAA SL+ QA LA VV FKL Sbjct: 481 TQQNAALVEQAAAAADSLKGQASNLAAVVGKFKL 514 >gi|300313024|ref|YP_003777116.1| methyl-accepting chemotaxis protein [Herbaspirillum seropedicae SmR1] Length = 571 Score = 568 bits (1465), Expect = e-163, Method: Compositional matrix adjust. Identities = 306/514 (59%), Positives = 397/514 (77%) Query: 1 MRWFYNLKIAKKLVLSFLAVIILTAGLGAFSIVELIKVNRASSDIATNWMPSVKVGLQLQ 60 M WFYNLKIA KL +FL V++ GLG FS +EL VN++S+DIA NW+PS+ ++ Sbjct: 1 MNWFYNLKIAHKLNAAFLVVLVFIIGLGGFSSLELAAVNKSSTDIARNWLPSIDALSKMS 60 Query: 61 ASMSRFRISELQHILATEEKDYDDAEKAMKTRMEIIRKDLASYEQMISEPEEKVLYPQFQ 120 +++R R ++Q +L ++++ + + +M + K++A Y +++SEP EK LYP+ + Sbjct: 61 LALARSRSFDMQQLLTADKQEEKASRERADKQMATLLKEVARYGKLVSEPREKELYPEIE 120 Query: 121 QTMQKYLAENKKIMELSRANQKDEARLQFKGESNKLFRQAVEQLGGLVKINDDGTVRSNA 180 + + Y ++++ L +QK A S ++R+ EQ+G L ++N G S+A Sbjct: 121 RNIALYKEAHERMSALFEQDQKQAAHALMNSTSTPVYRKLQEQIGELSEVNSKGAALSDA 180 Query: 181 EADHTFNEARNWILGLLIAIVVIAMTLAVWVARIVSVPLNKAVDVAQRVAEGDLGADIRS 240 +ADH + +A IL L+ VV+AM L+ W+AR+V+ PL AVD+A++VA GDL ADI + Sbjct: 181 DADHIYGQANMAILALVTTCVVLAMALSWWIARLVARPLANAVDIARQVAAGDLTADIPT 240 Query: 241 DSRDETGQLMDSLKAMNDSLLRIVGEVRTGTDTIATASTEIASGNLDLSSRTEQQAGSLE 300 RDETGQL+++L+ MN +LL IV EVR GTDTI TAS EIA+GN+DLSSRTEQQAG+LE Sbjct: 241 AHRDETGQLLEALRQMNGNLLNIVREVRQGTDTITTASAEIATGNMDLSSRTEQQAGALE 300 Query: 301 ETASAMEELTSTVKANADNARQANQLAVSASEVAQQGGAVVGQVVDTMGSINESSRKIVD 360 ETASAMEELTSTV+ N+DNARQANQLAV+ASEVAQ GG VVG+VV TM +IN+SSRKIVD Sbjct: 301 ETASAMEELTSTVRQNSDNARQANQLAVNASEVAQAGGTVVGKVVATMSAINDSSRKIVD 360 Query: 361 IISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSAAAAKEIKVLIDD 420 II VIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVR+LAQRSAAAA EIK LIDD Sbjct: 361 IIGVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRTLAQRSAAAAHEIKALIDD 420 Query: 421 SVEKVGSGSKLVEQAGVTMSEVVASVRRVTDIVGEISAASTEQSTGIEEVNRAITQMDEV 480 SV KV +GSKLV++AG TM+EVVASV+RV+DIV EI+AA EQ+ GIE++N A+ QMDE Sbjct: 421 SVAKVDTGSKLVDEAGQTMNEVVASVKRVSDIVAEITAAGAEQTQGIEQINNAVVQMDEN 480 Query: 481 TQQNAALVEQAAAAAQSLQEQAGKLAQVVSVFKL 514 TQQNAALVEQAAAAA++LQEQAG+L++ VSVF+L Sbjct: 481 TQQNAALVEQAAAAAKALQEQAGRLSETVSVFRL 514 >gi|300309735|ref|YP_003773827.1| methyl-accepting chemotaxis sensory transducer protein [Herbaspirillum seropedicae SmR1] Length = 567 Score = 544 bits (1402), Expect = e-156, Method: Compositional matrix adjust. Identities = 296/521 (56%), Positives = 395/521 (75%), Gaps = 13/521 (2%) Query: 1 MRWFYNLKIAKKLVLSFLAVIILTAGLGAFSIVELIKVNRASSDIATNWMPSVKVGLQLQ 60 M WFYN +IA KL+L+F V++LT GLG FSIV+L KVN+A++++ATNW+PS++ ++ Sbjct: 1 MNWFYNRRIATKLLLAFACVLLLTGGLGIFSIVQLHKVNQAATELATNWLPSIRSLNDMK 60 Query: 61 ASMSRFRISELQHILATEEKDYDDAEKAMKTRMEIIRKDLAS-------YEQMISEPEEK 113 ++SR R ++ Q L YD EK + ++ K+L + Y ISEP EK Sbjct: 61 VALSRVRANQAQLAL------YDGDEKQSSAVIGLMNKNLEALAEARKQYTGQISEPGEK 114 Query: 114 VLYPQFQQTMQKYLAENKKIMELSRANQKDEARLQFKGESNKLFRQAVEQLGGLVKINDD 173 L+P + K++A + + RA + DEA+ G + + + + L L ++N D Sbjct: 115 ALFPGVNARIDKFIALHGDTLAAVRAGKLDEAKRLLLGPAFENYLSLLNDLDKLAQVNLD 174 Query: 174 GTVRSNAEADHTFNEARNWILGLLIAIVVIAMTLAVWVARIVSVPLNKAVDVAQRVAEGD 233 G+V+S A+ T+++A+ W++ +LI ++ + LA+++AR+++ PL A + A +A GD Sbjct: 175 GSVQSTRSAETTYDQAQVWVVAILIGALLAGLALALFIARVIATPLRSAGEFAHLIAAGD 234 Query: 234 LGADIRSDSRDETGQLMDSLKAMNDSLLRIVGEVRTGTDTIATASTEIASGNLDLSSRTE 293 L ADI + S DETGQL +L AMN SL RIV +VR+GTD IATAS +I+SGNLDLSSRTE Sbjct: 235 LTADITARSDDETGQLTAALAAMNTSLRRIVSQVRSGTDAIATASGQISSGNLDLSSRTE 294 Query: 294 QQAGSLEETASAMEELTSTVKANADNARQANQLAVSASEVAQQGGAVVGQVVDTMGSINE 353 +QA SLEETASAMEELTSTV+ NA+NARQANQLA+SAS +A++GG VV QV++TMGSI+ Sbjct: 295 EQASSLEETASAMEELTSTVRQNAENARQANQLALSASTIAEEGGKVVSQVIETMGSIDA 354 Query: 354 SSRKIVDIISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSAAAAKE 413 SS+KIVDIISVID IAFQTNILALNAAVEAARAGEQGRGFAVVA+EVRSLAQRSA AAKE Sbjct: 355 SSKKIVDIISVIDSIAFQTNILALNAAVEAARAGEQGRGFAVVATEVRSLAQRSATAAKE 414 Query: 414 IKVLIDDSVEKVGSGSKLVEQAGVTMSEVVASVRRVTDIVGEISAASTEQSTGIEEVNRA 473 IK LID+SV++VG GS LV++AGVT+SEVV+SV+RVTD++ EISAAS EQ+ GIE+VN+A Sbjct: 415 IKTLIDNSVQQVGLGSGLVQKAGVTISEVVSSVQRVTDVMSEISAASGEQTQGIEQVNQA 474 Query: 474 ITQMDEVTQQNAALVEQAAAAAQSLQEQAGKLAQVVSVFKL 514 ITQMDEVTQQNAALVE+AA+A+QSL QA +L+++V VF+L Sbjct: 475 ITQMDEVTQQNAALVEEAASASQSLSHQAQRLSELVGVFRL 515 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.313 0.126 0.324 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,067,956 Number of extensions: 35056 Number of successful extensions: 542 Number of sequences better than 1.0e-08: 70 Number of HSP's gapped: 260 Number of HSP's successfully gapped: 72 Length of query: 516 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 427 Effective length of database: 1,198,455 Effective search space: 511740285 Effective search space used: 511740285 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 133 (55.8 bits)