BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0004.0139.p
         (264 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300309579|ref|YP_003773671.1| metal-dependent hydrolase [Herb...   412   e-116
gi|300311792|ref|YP_003775884.1| metal-dependent hydrolase [Herb...    78   7e-16

>gi|300309579|ref|YP_003773671.1| metal-dependent hydrolase
           [Herbaspirillum seropedicae SmR1]
          Length = 265

 Score =  412 bits (1059), Expect = e-116,   Method: Compositional matrix adjust.
 Identities = 201/264 (76%), Positives = 227/264 (85%), Gaps = 1/264 (0%)

Query: 1   MKLRIATYNIHKGVTSFSGRPRIHALKQALAQLDADLVFLQEVQGRHDRIALRHAALWPQ 60
           MKLR+ATYNIHKGVTSF+GRPRI ALKQALAQLD D++FLQEVQGRHD  ALRHA+ WPQ
Sbjct: 1   MKLRVATYNIHKGVTSFAGRPRILALKQALAQLDPDILFLQEVQGRHDLNALRHASNWPQ 60

Query: 61  QAQHEYLAGESHHAAYGVNAVYDHGHHGNALLSRFSIASARNQDVSDHAYESRGILHCVV 120
           Q QHEYLAG++HHAAYG+NAVYDHGHHGNAL+SRF IAS RNQDVSDHAYESRGILHCVV
Sbjct: 61  QGQHEYLAGDTHHAAYGMNAVYDHGHHGNALISRFEIASIRNQDVSDHAYESRGILHCVV 120

Query: 121 EIEGVPVHCYVIHLGLFAGGRRRQTAALIEAVRASSPPDAPVIIAGDFNDWGNKLSEFLR 180
            +EGV VHCYVIHLGLFAGGRRRQ  ALI+AV  SSPPDAP++IAGDFNDW N+LS  L 
Sbjct: 121 PVEGVEVHCYVIHLGLFAGGRRRQIDALIQAVSTSSPPDAPLLIAGDFNDWTNQLSRTLY 180

Query: 181 IRLGVSEVFEQ-HGSAHGLMGYLRKLGGMRPVIQPARTFPASMPMLRLDRIYLRGFEVES 239
             LGV+EVF+Q   +A G+M  LR+LGG+   I+PARTFPA MP LRLDRIY+RGF VE+
Sbjct: 181 QNLGVTEVFDQPQPNARGVMQGLRRLGGLSAPIRPARTFPAMMPWLRLDRIYVRGFRVET 240

Query: 240 AEVMRGGLWANLSDHAPIVSTLRL 263
           AEV++ G WA LSDHAPIV+ L L
Sbjct: 241 AEVLKDGPWARLSDHAPIVTELHL 264


>gi|300311792|ref|YP_003775884.1| metal-dependent hydrolase
           [Herbaspirillum seropedicae SmR1]
          Length = 260

 Score = 78.2 bits (191), Expect = 7e-16,   Method: Compositional matrix adjust.
 Identities = 80/263 (30%), Positives = 112/263 (42%), Gaps = 48/263 (18%)

Query: 3   LRIATYNIHKGVTSFSGRPRIHALKQALAQLDADLVFLQEVQ-GRHDRIALRHAALWPQQ 61
           L +ATYNIH G     GR     +   L +L AD+V LQEV  G  D++           
Sbjct: 36  LTVATYNIH-GAVGTDGRFAPERVAAVLDELQADVVALQEVPLGGADQV----------- 83

Query: 62  AQHEYLAGESHH----AAYGVNAVYDHGHHGNALLSRFSIASARNQDVSDHAYESRGILH 117
              + LA    H    AA G         +GNA+LSR+ I +  + D+S  + E RG L 
Sbjct: 84  ---DVLALLRAHTGMFAAEGPTMESPARRYGNAVLSRYPILATESIDLSFGSREPRGALD 140

Query: 118 CVVEIEGVPVHCYVIHLGLFAGGRRRQTAALIEAVRASSPPDAPVIIAGDFNDWGNKLSE 177
             ++  G  +     HLGL    RR Q   L++A   +   DAPVI+ GD N+W      
Sbjct: 141 ADIDCRGQLLRVVATHLGLKPAERRAQIKRLLQAFDTN---DAPVILLGDVNEW------ 191

Query: 178 FLRIRLGVSEVFEQHGSAHGLMGYLRKLGGMRPVIQPARTFPASMPMLRLDRIYLRG-FE 236
           F+  R                   LR L G         TFP+  P+  LDRI++     
Sbjct: 192 FMWGR------------------TLRWLVGHFEAAPAPPTFPSRWPVFALDRIWISPRRR 233

Query: 237 VESAEVMRGGLWANLSDHAPIVS 259
           ++   V R  L    SDH P+++
Sbjct: 234 LQHLAVHRSALARVASDHLPLLA 256


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.324    0.138    0.419 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 846,776
Number of extensions: 35097
Number of successful extensions: 101
Number of sequences better than 1.0e-08: 2
Number of HSP's gapped: 96
Number of HSP's successfully gapped: 2
Length of query: 264
Length of database: 1,619,870
Length adjustment: 83
Effective length of query: 181
Effective length of database: 1,226,865
Effective search space: 222062565
Effective search space used: 222062565
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (22.0 bits)
S2: 130 (54.7 bits)