BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0004.0132.p (596 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309596|ref|YP_003773688.1| acyl-CoA dehydrogenase [Herbasp... 1126 0.0 gi|300313303|ref|YP_003777395.1| acyl-CoA dehydrogenase [Herbasp... 519 e-148 gi|300310835|ref|YP_003774927.1| acyl-CoA dehydrogenase [Herbasp... 473 e-134 gi|300310260|ref|YP_003774352.1| acyl-CoA dehydrogenase [Herbasp... 137 2e-33 gi|300311865|ref|YP_003775957.1| acyl-CoA dehydrogenase [Herbasp... 101 2e-22 >gi|300309596|ref|YP_003773688.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 596 Score = 1126 bits (2912), Expect = 0.0, Method: Compositional matrix adjust. Identities = 525/596 (88%), Positives = 570/596 (95%) Query: 1 MPQYNAPLRDMQFVLHELLKVEDVLKTLPAHTDTNRELIDQVLEEGGKFTSQVLFPLNHS 60 MPQYNAPLRDMQFVLHELL VE+V K LP H DTNREL+DQVLEEGGKF SQVLFPLNHS Sbjct: 1 MPQYNAPLRDMQFVLHELLNVEEVFKELPPHADTNRELVDQVLEEGGKFASQVLFPLNHS 60 Query: 61 GDREGCTYNKETKTVTPPKGYKEAYKQYVEAGWAALSCDPEFGGQGLPLVMNNSFYEMLN 120 GDREGC Y++E+KTVT PKG+K+AY+QYVEAGWAALSCDPEFGGQGLPL +NNSFYEMLN Sbjct: 61 GDREGCHYDRESKTVTTPKGFKQAYQQYVEAGWAALSCDPEFGGQGLPLTINNSFYEMLN 120 Query: 121 SSNQAWTMYPGLSHGAYECIHAHGSDEQKALYLPKLISGEWTGTMCLTEAHCGTDLGMLR 180 SSNQAWTMYPGLSHGAYEC+HAHGS+EQK LYLPKL+SGEWTGTMCLTEAHCGTDLG+LR Sbjct: 121 SSNQAWTMYPGLSHGAYECLHAHGSEEQKQLYLPKLVSGEWTGTMCLTEAHCGTDLGLLR 180 Query: 181 SKAEPQADGSYKITGSKIFISAGEHDMSDNIVHLVLARLPDAPQGSKGISLFIVPKFMPN 240 SKAEPQADGSYKITG+KIFISAGE+D+S NI+HLVLARLPDAP+GSKGISLF+VPKF+PN Sbjct: 181 SKAEPQADGSYKITGNKIFISAGENDVSANIIHLVLARLPDAPEGSKGISLFLVPKFLPN 240 Query: 241 ADGSLGARNPIACGAIEEKMGIHGNATCQMNLDGAVGWLIGGPHKGLNAMFVFMNAARLG 300 ADGSLGARNPI CGAIEEKMGIHGNATCQMNLDGA GWLIG PHKGL AMFVFMNAARLG Sbjct: 241 ADGSLGARNPIFCGAIEEKMGIHGNATCQMNLDGATGWLIGQPHKGLQAMFVFMNAARLG 300 Query: 301 VGMQGLGLTEVAYQNALVYAKDRIQMRSLSGVKAPDKAADPIIVHPDVRRMLLTAKAYAE 360 VGMQGLGLTEVAYQNALVYAKDR+QMRSLSG+KA DK ADPIIVHPDVRRMLLTAKA+AE Sbjct: 301 VGMQGLGLTEVAYQNALVYAKDRLQMRSLSGIKAADKPADPIIVHPDVRRMLLTAKAFAE 360 Query: 361 GGRAFCSYVALQLDKELNHPDEEVRKEAADQVALLTPIVKAFITDNAWIATSECLQVYGG 420 GGRAF SYVAL+LDKEL HPDE+VRK++AD VALLTP+VKAF+TDNAW+ATSECLQVYGG Sbjct: 361 GGRAFTSYVALELDKELTHPDEQVRKDSADIVALLTPVVKAFLTDNAWMATSECLQVYGG 420 Query: 421 HGYISEWGMEQYVRDARINMIYEGTNTIQSLDLLGRKILMDNGAKLKKFGAQVQAFIEDN 480 HGYI+EWGMEQYVRDARINMIYEGTNTIQSLDLLGRKILMDNGAKLKKFGAQ+QAF+E++ Sbjct: 421 HGYIAEWGMEQYVRDARINMIYEGTNTIQSLDLLGRKILMDNGAKLKKFGAQIQAFVEEH 480 Query: 481 GTDEAMSEFISPLADIGDKVSKLTMEIGMKAFQNPDEVGAAAVPYLRVVGHLVYAYFFAQ 540 GTDE+MSEFI+PLADIGDKV+KLTMEIGMK+F N DE GAAAVPYLRVVGHLV++Y FA+ Sbjct: 481 GTDESMSEFITPLADIGDKVTKLTMEIGMKSFANQDEAGAAAVPYLRVVGHLVFSYLFAR 540 Query: 541 MAKIALEKQDSGDSFYKSKLATARFYFARLLPETATLIRQARSGSANLMALDADLF 596 MAKIALEKQDSGDSFY +KLATARFYFARLLPETA LIRQARSG+ANLMALDA+LF Sbjct: 541 MAKIALEKQDSGDSFYAAKLATARFYFARLLPETAMLIRQARSGAANLMALDAELF 596 >gi|300313303|ref|YP_003777395.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 591 Score = 519 bits (1337), Expect = e-148, Method: Compositional matrix adjust. Identities = 271/583 (46%), Positives = 365/583 (62%), Gaps = 31/583 (5%) Query: 3 QYNAPLRDMQFVLHELLKVEDVLKTLPAHTDTNRELIDQVLEEGGKFTSQVLFPLNHSGD 62 Y PLRD FVL E+L+ VL+ LPAH + + +L+ QVLEE G+F + V+ PLN GD Sbjct: 4 HYAPPLRDFAFVLDEVLQAPQVLRALPAHAEIDGDLMMQVLEEAGRFAADVVAPLNGIGD 63 Query: 63 REGCTYNKETKTVTPPKGYKEAYKQYVEAGWAALSCDPEFGGQGLPLVMNNSFYEMLNSS 122 REGC + + TVT P G++ AY Q+ GW AL+C PE GGQGLP V++ + YEML+++ Sbjct: 64 REGCRF--KNGTVTTPPGFERAYAQFCAGGWPALACAPEHGGQGLPHVLDCALYEMLSAA 121 Query: 123 NQAWTMYPGLSHGAYECIHAHGSDEQKALYLPKLISGEWTGTMCLTEAHCGTDLGMLRSK 182 N WTMYPGL HGAY C+ AHGS +A YL K+ SGEW TMCLTEA G+DLG LR++ Sbjct: 122 NHGWTMYPGLLHGAYACLAAHGSAALQARYLDKIASGEWLATMCLTEAQAGSDLGQLRTR 181 Query: 183 AEPQADGSYKITGSKIFISAGEHDMSDNIVHLVLARLPDAPQGSKGISLFIVPKFMPNAD 242 A PQ DGSY+++G KIFIS GEHD++ NIVHLVLARLPDAP GSKG+SLF+ P+ + + Sbjct: 182 AVPQDDGSYRLSGDKIFISGGEHDLTPNIVHLVLARLPDAPAGSKGLSLFLAPRVLDD-- 239 Query: 243 GSLGARNPIACGAIEEKMGIHGNATCQMNLDGAVGWLIGGPHKGLNAMFVFMNAARLGVG 302 G+RN + C IE KMGIHG+ATC ++ + A WL+G +GL AMFV MNAARL VG Sbjct: 240 ---GSRNGVHCTGIEHKMGIHGSATCSLHFEQAQAWLVGEAGRGLAAMFVMMNAARLHVG 296 Query: 303 MQGLGLTEVAYQNALVYAKDRIQMRSLSGVKAPDKAADPIIV-HPDVRRMLLTAKAYAEG 361 QGLGL E AYQ A YA++R Q R AP + + +IV HP V+++L+ + EG Sbjct: 297 AQGLGLAEAAYQRASAYARERRQSR------APGSSGNDLIVHHPAVQKLLMEQRCRIEG 350 Query: 362 GRAFCSYVALQLDKELNHPDEEVRKEAADQVALLTPIVKAFITDNAWIATSECLQVYGGH 421 R + L LD + P+ +R +++ +TP++KAF+TD + S LQV+GGH Sbjct: 351 ARLLTCWAGLLLDLAEHDPEAALRSRHHERLEFITPVIKAFLTDQGFQCASRALQVFGGH 410 Query: 422 GYISEWGMEQYVRDARINMIYEGTNTIQSLDLLGRKILMDNGAKLKKFGAQVQAFI-EDN 480 GY+ E G+EQ++RDARI MIYEGTN IQ++D L RK+L D G +L F V+A I E + Sbjct: 411 GYVVETGIEQFMRDARITMIYEGTNEIQAIDFLMRKVLGDEGRRLDDFLGLVRADIAEAS 470 Query: 481 GTDEAMSEFISPLADIGDKVSKLTMEIGMKAFQNPDEVGAAAVPY------LRVVGHLVY 534 G E +G+ V KL + + A A+PY LR+ GH+ Sbjct: 471 GLGEQARL-------LGEAVDKLRI---FASRIAAAAAQAPALPYYVADEMLRLCGHVAL 520 Query: 535 AYFFAQMAKIALEKQDSGDSFYKSKLATARFYFARLLPETATL 577 + + + + AL +Y K A ++FA + E L Sbjct: 521 GWMWLRAGRRALSAMAQDPLWYGGKRDAACYHFAFVFAEVQQL 563 >gi|300310835|ref|YP_003774927.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 594 Score = 473 bits (1216), Expect = e-134, Method: Compositional matrix adjust. Identities = 260/599 (43%), Positives = 362/599 (60%), Gaps = 13/599 (2%) Query: 4 YNAPLRDMQFVLHELLKVEDVLKTLPAHTDTNRELIDQVLEEGGKFTSQVLFPLNHSGDR 63 Y AP++DM FV++EL + V LP D E + +LEE KF S+V+ PLNHS D+ Sbjct: 3 YTAPVKDMLFVMNELAGLAAV-NALPGCEDATPETAEAILEENAKFCSEVVAPLNHSADK 61 Query: 64 EGCTYNKETKTVTPPKGYKEAYKQYVEAGWAALSCDPEFGGQGLPLVMNNSFYEMLNSSN 123 E ++ T +P G+KEA++Q+ EAGW + EFGGQGLP ++ EMLNS+N Sbjct: 62 EPSSWADGKVTTSP--GFKEAFRQFGEAGWQGVQHPVEFGGQGLPKLLATPCIEMLNSAN 119 Query: 124 QAWTMYPGLSHGAYECIHAHGSDEQKALYLPKLISGEWTGTMCLTEAHCGTDLGMLRSKA 183 ++ + P L+ G E + G+ +QK ++ ISG+WTGTM LTE G+DL ++R++A Sbjct: 120 LSFALCPLLTDGTIEALMTAGTQQQKETFIANFISGKWTGTMNLTEPQAGSDLALVRTRA 179 Query: 184 EPQADGSYKITGSKIFISAGEHDMSDNIVHLVLARLPDAPQGSKGISLFIVPKFMPNADG 243 P+ DG+YK+ G+KIFI+ GEHDM++NIVHLVLAR P+AP+G KGISLFIVPKF+ NADG Sbjct: 180 VPEGDGTYKLFGTKIFITYGEHDMAENIVHLVLARTPNAPEGVKGISLFIVPKFLVNADG 239 Query: 244 SLGARNPIACGAIEEKMGIHGNATCQMNL---DGAVGWLIGGPHKGLNAMFVFMNAARLG 300 SLGARN + C +IE K+GI + T + GA+G L+G ++GL MF+ MNAAR Sbjct: 240 SLGARNDVHCVSIEHKLGIKASPTAVLQFGDHGGAIGTLVGEENRGLEYMFIMMNAARFA 299 Query: 301 VGMQGLGLTEVAYQNALVYAKDRIQMRSLSGVKAPDKAADPIIVHPDVRRMLLTAKAYAE 360 VG+QGL ++E AYQ A+ YA+DRIQ R L+G P II PDVRRML++ +A E Sbjct: 300 VGIQGLSVSERAYQKAVAYARDRIQSRDLAGSPGPVA----IIHQPDVRRMLMSMRAQIE 355 Query: 361 GGRAFCSYVALQLDKELNHPDEEVRKEAADQVALLTPIVKAFITDNAWIATSECLQVYGG 420 RA A D DE RK L PIVK +IT+ + TS +QV+GG Sbjct: 356 AARALSYVAAATSDAAHFSDDEATRKSNQAFYEYLVPIVKGWITEMSIDVTSTGVQVHGG 415 Query: 421 HGYISEWGMEQYVRDARINMIYEGTNTIQSLDLLGRKILMDNGAKLKKFGAQVQAFIEDN 480 G+I E G QY RDARI IYEGT IQ+ DL+GRK D GA K AQV+ + + Sbjct: 416 MGFIEETGAAQYYRDARILPIYEGTTAIQANDLVGRKTARDGGAVAKGILAQVRKTVSEL 475 Query: 481 GTDEAMSEFISPLADIGDKVSKLTMEIGMKAFQNPDEVGAAAVPYLRVVGHLVYAYFFAQ 540 D + + L + D + ++ + + V A +VPYL++ G ++ + A+ Sbjct: 476 QGDAQLQPIAARLGEAADALEQVVDYVVANVKSDIKAVFAGSVPYLKMAGVVLGGWQMAR 535 Query: 541 MAKIALEKQDSGD---SFYKSKLATARFYFARLLPETATLIRQARSGSANLMALDADLF 596 A ++ K +G+ FY++KLATARFY ++LP+ G A +MAL + F Sbjct: 536 AAAVSAAKLKAGEGDGKFYEAKLATARFYADQILPQALAYRTAIVDGGAAVMALAEEQF 594 >gi|300310260|ref|YP_003774352.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 382 Score = 137 bits (346), Expect = 2e-33, Method: Compositional matrix adjust. Identities = 118/384 (30%), Positives = 174/384 (45%), Gaps = 46/384 (11%) Query: 83 EAYKQYVEAGWAALSCDPEFGGQGLP-LVMNNSFYEMLNSSNQAWTMYPGLSHGAYECIH 141 EA K+ E G + ++GG G+ + + + E+ T+ + A Sbjct: 38 EALKELAELGAMGMCVPEQWGGAGMDYMSLVLALEEIAAGDGATSTIVSVQNSLACGITE 97 Query: 142 AHGSDEQKALYLPKLISGEWTGTMCLTEAHCGTDLGMLRSKAEPQADGSYKITGSKIFIS 201 +GS QK +L L GE G CLTE H G+D +R++AE D + + G+K FI+ Sbjct: 98 KYGSPTQKEQWLKPLARGEMLGCFCLTEPHTGSDAAAIRTRAERDGD-DFILNGTKQFIT 156 Query: 202 AGEHDMSDNIVHLVLARLPDAPQGSKGISLFIVPKFMPNADGSLGARNPIACGAIEEKMG 261 +G+H V +V A + D G KGIS F+VP P G EEKMG Sbjct: 157 SGKHAG----VAIVFA-VTDRSAGKKGISCFLVPCDTPG----------FVVGRSEEKMG 201 Query: 262 IHGNATCQMNLDGA---VGWLIGGPHKGLNAMFVFMNAARLGVGMQGLGLTEVAYQNALV 318 H + T Q+ LD L+G +G + A R+G+ Q +G+ A++ A+ Sbjct: 202 QHASDTVQIMLDNCRVPATALLGKEGEGYRIALSNLEAGRIGIAAQSVGMARAAFEAAVS 261 Query: 319 YAKDRIQMRSLSGVKAPDKAADPIIVHPDVRRMLLTAKAYAEGGRAFCSYVALQLDKELN 378 YA+ R GV PII H V L + R + A QL K+ Sbjct: 262 YARQRESF----GV--------PIIEHQAVNFRLADMNTLLDAARLMV-WRAAQL-KDQG 307 Query: 379 HPDEEVRKEAADQVALLTPIVKAFITDNAWIATSECLQVYGGHGYISEWGMEQYVRDARI 438 P KEA+ + K F ++ A S+ +Q++GG GY S++ +E+ RD RI Sbjct: 308 RP---CLKEAS--------MAKMFASEAAEKIASDAIQIHGGVGYTSDFPVERIYRDVRI 356 Query: 439 NMIYEGTNTIQSLDLLGRKILMDN 462 IYEG N IQ L ++GR I D Sbjct: 357 CQIYEGANDIQRL-VIGRAIASDQ 379 >gi|300311865|ref|YP_003775957.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 386 Score = 101 bits (251), Expect = 2e-22, Method: Compositional matrix adjust. Identities = 98/374 (26%), Positives = 157/374 (41%), Gaps = 47/374 (12%) Query: 90 EAGWAALSCDPEFGGQGLPLVMNNSFYEMLNSSNQAWTMYPGLSHGAYECIHAHGSDEQK 149 E G+ L GG GL + +E L + + Y + + I + G K Sbjct: 47 ELGFCGLYTPEADGGLGLSRLDATVVFEELARACTSTAAYLTIHNMVSWMIASWGQPSLK 106 Query: 150 ALYLPKLISGEWTGTMCLTEAHCGTDLGMLRSKAEPQADGSYKITGSKIFIS-AGEHDMS 208 A + +L +G G+ CLTE G+D L++ A + DG Y + GSK FIS AG D Sbjct: 107 AQWCEQLAAGRKIGSYCLTEPGSGSDAASLKTTARLE-DGHYVLNGSKAFISGAGSTD-- 163 Query: 209 DNIVHLVLARLPDAPQGSKGISLFIVPKFMPNADGSLGARNPIACGAIEEKMGIHGNATC 268 V +V+AR +G++G+S +VP P ++ G E KMG + T Sbjct: 164 ---VLVVMART--GGEGARGVSAIVVPGNAPG----------VSYGKKESKMGWNSQPTR 208 Query: 269 QMNLDGA---VGWLIGGPHKGLNAMFVFMNAARLGVGMQGLGLTEVAYQNALVYAKDRIQ 325 ++ D L+G +G ++ R+ + +G + A A Y K+R Q Sbjct: 209 TISFDNVKVPADHLLGQEGQGFIFAMKGLDGGRINIATCSVGAAQAALDAAHAYMKERKQ 268 Query: 326 MRSLSGVKAPDKAADPIIVHPDVRRMLLTAKAYAEGGRAFCSYVALQLDKELNHPDEEVR 385 PI ++ L + R A +LD Sbjct: 269 F------------GRPIADFQALQFKLADMQTELVAARQMVRLAATKLDA---------- 306 Query: 386 KEAADQVALLTPIVKAFITDNAWIATSECLQVYGGHGYISEWGMEQYVRDARINMIYEGT 445 + + + K TD + +E LQ++GG+GYI E+ +E+Y RD R++ I EGT Sbjct: 307 --GSPEATTYCAMAKRLATDLGFRVCNEALQLHGGYGYIREYPLERYFRDVRVHQILEGT 364 Query: 446 NTIQSLDLLGRKIL 459 N I + ++ RK+L Sbjct: 365 NEIMRV-IISRKLL 377 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,923,861 Number of extensions: 83033 Number of successful extensions: 217 Number of sequences better than 1.0e-08: 8 Number of HSP's gapped: 197 Number of HSP's successfully gapped: 8 Length of query: 596 Length of database: 1,619,870 Length adjustment: 91 Effective length of query: 505 Effective length of database: 1,188,985 Effective search space: 600437425 Effective search space used: 600437425 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 133 (55.8 bits)