BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0004.0131.p (795 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309597|ref|YP_003773689.1| 3-hydroxyacyl-CoA dehydrogenase... 1496 0.0 gi|300313418|ref|YP_003777510.1| 3-hydroxyacyl-CoA dehydrogenase... 117 3e-27 gi|300313811|ref|YP_003777903.1| 3-hydroxyacyl-CoA dehydrogenase... 70 9e-13 >gi|300309597|ref|YP_003773689.1| 3-hydroxyacyl-CoA dehydrogenase oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 805 Score = 1496 bits (3873), Expect = 0.0, Method: Compositional matrix adjust. Identities = 733/795 (92%), Positives = 769/795 (96%) Query: 1 VSNFIVKKVAVLGAGVMGAQIAAHCINAKVPVVLFDLPAKEGPKNGIVQRAIENLKKLSP 60 VSNFIVKKVAVLGAGVMGAQIAAHCINA+VPVVLFDLPAKEGPKNGIVQRAIENLKKLSP Sbjct: 11 VSNFIVKKVAVLGAGVMGAQIAAHCINARVPVVLFDLPAKEGPKNGIVQRAIENLKKLSP 70 Query: 61 APLGNKEDASLIQVANYEDDIELLKGCDLIIEAIAERMDWKHDLYKKVAPHIAPNAIFAS 120 APLGNK+DA+LIQVANYEDD+ +L+GCDLIIEAIAERMDWKHDLYKKVAPHI P AIFAS Sbjct: 71 APLGNKDDAALIQVANYEDDLAVLEGCDLIIEAIAERMDWKHDLYKKVAPHIGPKAIFAS 130 Query: 121 NTSGLSITALSDGFDAELKARFCGVHFFNPPRYMHLVELIPTATTNPEILNQLEAFLTTT 180 NTSGLSI ALS+GFDA LKARFCGVHFFNPPRYMHLVELIPT +T P IL+QLEAFLTTT Sbjct: 131 NTSGLSINALSEGFDAGLKARFCGVHFFNPPRYMHLVELIPTQSTEPHILDQLEAFLTTT 190 Query: 181 LGKGVVRAFDTPNFVANRVGVFGILATVIEAEKYGLSVDVVDDLTGAKLGRAKSGTFRTA 240 LGKGVVRAFDTPNFVANRVGVFGILAT+IEAEKYGLSVDVVDDLTGAKLGRAKSGTFRTA Sbjct: 191 LGKGVVRAFDTPNFVANRVGVFGILATIIEAEKYGLSVDVVDDLTGAKLGRAKSGTFRTA 250 Query: 241 DVVGLDTMGHVIKTMQDNLKDDPFFSSYATPPLLAKLVEQGALGQKSGAGFYKKVGKDIL 300 DVVGLDTMGHVI+TMQDNLKDDPFFSSYATPPLLAKLVEQGALGQKSGAGFYKKVGKDIL Sbjct: 251 DVVGLDTMGHVIRTMQDNLKDDPFFSSYATPPLLAKLVEQGALGQKSGAGFYKKVGKDIL 310 Query: 301 RIDPAKGDYVPGGGKADDIIARILKEKDPVKRMKALHDSTNPQGQFLWAIFRDAFHYIAV 360 RIDPAKGDYVPGG KADDIIARILKEKDPVKRMKALHDSTNPQGQFLWAIFRDAFHYIAV Sbjct: 311 RIDPAKGDYVPGGAKADDIIARILKEKDPVKRMKALHDSTNPQGQFLWAIFRDAFHYIAV 370 Query: 361 HLETVADNARDLDFAMRWGFGWSVGPFETWQASGWQQVATWIKEDIDAGKALSTAPLPAW 420 HLE+VADNARDLDFAMRWGFGWSVGPFETWQA+GW+Q+A W+KEDIDAGKALSTAPLP W Sbjct: 371 HLESVADNARDLDFAMRWGFGWSVGPFETWQAAGWKQIAQWVKEDIDAGKALSTAPLPDW 430 Query: 421 VFDGREGVHSAQGSWSPSKKTNVARSELPVYQRQAFRAPLVGEGAADGKTAGTTFFEDDS 480 VFDGR+GVHS +GS+SPSKK +VARSELPVYQRQAFRAPLVGEGAADGK+AGTTFFED+S Sbjct: 431 VFDGRDGVHSEKGSYSPSKKADVARSELPVYQRQAFRAPLVGEGAADGKSAGTTFFEDES 490 Query: 481 VRIWHQNDDVLILSFKTKMHVIGQGVIDGINKALDEAEKNFKGLVIWHADAAEGGAFSAG 540 VRIWHQNDDVLILSFKTKMHVIGQGVIDG+NKA+ EAE+NFKGLVIWH DAAEGGAFSAG Sbjct: 491 VRIWHQNDDVLILSFKTKMHVIGQGVIDGMNKAVSEAEQNFKGLVIWHPDAAEGGAFSAG 550 Query: 541 ADLQSMLPLFMSGGVKAIEPVVHHLQQAHQRLKYANVPVVAAVAGLALGGGCELLLHTAK 600 ADLQSMLPLFMSGGVKAIEPVVH LQQAHQRLKYA+VPV+AAVAGLALGGGCELLLHTAK Sbjct: 551 ADLQSMLPLFMSGGVKAIEPVVHQLQQAHQRLKYASVPVIAAVAGLALGGGCELLLHTAK 610 Query: 601 RVASIESYIGLVEVGVGLIPAGGGLKEAAVRAAREAKGNDILQFLKDYMLAAATANVSKS 660 RV SIESYIGLVEVGVGLIPAGGGLKEAAVRAAREAKGNDIL FLK+YMLAAATANVSKS Sbjct: 611 RVVSIESYIGLVEVGVGLIPAGGGLKEAAVRAAREAKGNDILPFLKEYMLAAATANVSKS 670 Query: 661 ALEARKLGYLKADDVIVFNPYELLHVAKVEARAMSDSGYRPALAPQVPVVGRYGVGTIMA 720 ALEARKLGYL+ DDVIVFNPYELLHVAKVEARA+ D+GYRP L PQVPV+GR G+ TIMA Sbjct: 671 ALEARKLGYLREDDVIVFNPYELLHVAKVEARALFDAGYRPQLPPQVPVIGRNGLATIMA 730 Query: 721 QLVNMRDGGFISAHDFKLGQMIAEIVSGGEVEEGSVVNEQWLLDLERKAFMELLNHPKTQ 780 QLVNMRDGGFISAHDFKLG+MIAE VSGGEVEEGS+VNEQWLLD+ERK FMELLNHPKTQ Sbjct: 731 QLVNMRDGGFISAHDFKLGKMIAETVSGGEVEEGSIVNEQWLLDIERKFFMELLNHPKTQ 790 Query: 781 ERIMGMMQTGKPVRN 795 ERIMGMMQTGKPVRN Sbjct: 791 ERIMGMMQTGKPVRN 805 >gi|300313418|ref|YP_003777510.1| 3-hydroxyacyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 512 Score = 117 bits (294), Expect = 3e-27, Method: Compositional matrix adjust. Identities = 99/292 (33%), Positives = 143/292 (48%), Gaps = 22/292 (7%) Query: 9 VAVLGAGVMGAQIAAHCINAKVPVVLFDLPAKEGPKNGIVQRAIENLKKLSPAPLGNKED 68 VAV+G+G MGA IA A PV+L+D ++EG + NL KL +K Sbjct: 16 VAVIGSGTMGAGIAQVAAVAGHPVLLYD--SREGAAGKAIGAICTNLDKLVSK---DKLT 70 Query: 69 ASLIQVANYE----DDIELLKGCDLIIEAIAERMDWKHDLYKKVAPHIAPNAIFASNTSG 124 +L Q A+ +E L DL+IEAI E++ KH+L+ K+ ++ I A+NTS Sbjct: 71 PALAQAASQRISVAQSLEELHQADLVIEAIVEQLAAKHELFGKLEAIVSTRCILATNTSS 130 Query: 125 LSITALSDGFDAELKARFCGVHFFNPPRYMHLVELIPTATTNPEILNQLEAFLTTTL--G 182 LSIT ++ G + R G+HFFNP M LVE+I ++ + L T L G Sbjct: 131 LSITQIAAGL--QHPERVVGMHFFNPVPMMALVEIISGLASDAALAQCLH---DTALAWG 185 Query: 183 KGVVRAFDTPNFVANRVGVFGILATVIEAEKYGLSVDVVDDLTGAKLGRAKSGTFRTADV 242 K V A TP F+ NR+ A + G + D + G + G F D+ Sbjct: 186 KTPVMARSTPGFIVNRLA-RPYYAEGLRMLHEGGAQPATIDAVMREAGGFRMGPFELMDL 244 Query: 243 VGLDTMGHVIKTMQDNLKDDPFFSSYATPPLLAK-LVEQGALGQKSGAGFYK 293 +G D V +++ +DP F TP L+ + LV G LG+KSG GFY+ Sbjct: 245 IGHDVNFAVTQSVFHAYFNDPRF----TPSLIQQELVHAGYLGRKSGRGFYR 292 >gi|300313811|ref|YP_003777903.1| 3-hydroxyacyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 323 Score = 69.7 bits (169), Expect = 9e-13, Method: Compositional matrix adjust. Identities = 56/195 (28%), Positives = 91/195 (46%), Gaps = 8/195 (4%) Query: 6 VKKVAVLGAGVMGAQIAAHCINAKVPVVLFD-LPAKEGPKNGIVQRAIENLKKLSPAPLG 64 + +VAV+G GV+GA AA+ + + V D +P E + R +L+ L G Sbjct: 15 IARVAVVGTGVIGASWAAYFLAQGLEVSAVDPMPQAEQKLRAAIARHWPSLQAL-----G 69 Query: 65 NKEDASLIQVANYEDDIELLKGCDLIIEAIAERMDWKHDLYKKVAPHIAPNAIFASNTSG 124 E AS ++ Y+ L+G D + E ER D K DL++++ + + AS++SG Sbjct: 70 LAEHASQERLRFYDQLEAALEGADFVQENGPEREDLKIDLFRRMDAALPAQVVLASSSSG 129 Query: 125 LSITALSDGFDAELKARFCGVHFFNPPRYMHLVELIPTATTNPEILNQLEAFLTTTLGKG 184 L ++ + R H FNPP + LVE+I A T+PE + F K Sbjct: 130 LLMSKVQAA--CRHPQRVVLGHPFNPPHLIPLVEVIGGAQTSPEAVQTALDFYAAIGKKP 187 Query: 185 VVRAFDTPNFVANRV 199 + + +ANR+ Sbjct: 188 IHVRKEVKGHIANRL 202 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.136 0.402 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,481,179 Number of extensions: 108259 Number of successful extensions: 375 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 370 Number of HSP's successfully gapped: 4 Length of query: 795 Length of database: 1,619,870 Length adjustment: 93 Effective length of query: 702 Effective length of database: 1,179,515 Effective search space: 828019530 Effective search space used: 828019530 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 134 (56.2 bits)