BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0004.0130.p (331 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309598|ref|YP_003773690.1| acetyl-CoA acyltransferase [Her... 365 e-102 gi|300313287|ref|YP_003777379.1| beta-ketoadipyl-CoA thiolase [H... 218 5e-58 gi|300313421|ref|YP_003777513.1| 3-oxoadipyl-CoA thiolase [Herba... 129 3e-31 gi|300309581|ref|YP_003773673.1| acetyl-CoA acetyltransferase [H... 122 4e-29 gi|300310259|ref|YP_003774351.1| acetyl-CoA acetyltransferase [H... 110 2e-25 >gi|300309598|ref|YP_003773690.1| acetyl-CoA acyltransferase [Herbaspirillum seropedicae SmR1] Length = 398 Score = 365 bits (936), Expect = e-102, Method: Compositional matrix adjust. Identities = 174/189 (92%), Positives = 184/189 (97%) Query: 1 MTKQLQDAYIVAATRTPIGKAPRGMFKNVRPDDLLVRAIQSAIAQVPGLDPKLIEDAIIG 60 M+KQLQDAYIVAATRTPIGKAPRGMFKN RPDDLLVRAIQSA+AQVPGLDPKLIEDAI+G Sbjct: 1 MSKQLQDAYIVAATRTPIGKAPRGMFKNTRPDDLLVRAIQSAVAQVPGLDPKLIEDAIVG 60 Query: 61 CSFPEGAQGLNMARNAVLLAGLPNTIGGVTINRYCASGVTAIAMAADRIRVGQADVMIAG 120 CSFPEGAQGLNMARNAVLLAGLPNTIGGVTINRYCASG+TAIAMAADRIRVG+ADVMIA Sbjct: 61 CSFPEGAQGLNMARNAVLLAGLPNTIGGVTINRYCASGITAIAMAADRIRVGEADVMIAA 120 Query: 121 GAESMSMVPMMGFHPSVNMGAFKDENIGMAYGMGLTAERVAQQWKVSREAQDAFALASHQ 180 GAESMSMVPMMGFHPS+N+ AFKDEN+GMAYGMGLTAE+VAQQWKVSREAQD F+LASHQ Sbjct: 121 GAESMSMVPMMGFHPSININAFKDENVGMAYGMGLTAEKVAQQWKVSREAQDEFSLASHQ 180 Query: 181 KAIAAQLAG 189 KAIAAQ AG Sbjct: 181 KAIAAQEAG 189 Score = 267 bits (682), Expect = 1e-72, Method: Compositional matrix adjust. Identities = 128/139 (92%), Positives = 135/139 (97%) Query: 193 GALILVSEKILKQFNLTPLARFASFAVRGVPPEIMGIGPKEAIPVALRAAGITQDQLDWI 252 GALI+VSEKILKQFNLTPLARF SFAVRGVPPEIMGIGPKEAIP AL+A G+TQDQ+DWI Sbjct: 260 GALIIVSEKILKQFNLTPLARFVSFAVRGVPPEIMGIGPKEAIPAALKAGGLTQDQIDWI 319 Query: 253 ELNEAFAAQALAVIQDLGLDPTKVNPMGGAIALGHPLGATGAIRAATTIHAMRRKNLKYG 312 ELNEAFAAQALAVI DLGLDP+KVNPMGGAIALGHPLGATGAIRAATTIHA+RRKNLKYG Sbjct: 320 ELNEAFAAQALAVIGDLGLDPSKVNPMGGAIALGHPLGATGAIRAATTIHALRRKNLKYG 379 Query: 313 MVTMCVGTGMGAAGIFERM 331 MVTMCVGTGMGAAGIFER+ Sbjct: 380 MVTMCVGTGMGAAGIFERV 398 >gi|300313287|ref|YP_003777379.1| beta-ketoadipyl-CoA thiolase [Herbaspirillum seropedicae SmR1] Length = 400 Score = 218 bits (555), Expect = 5e-58, Method: Compositional matrix adjust. Identities = 152/401 (37%), Positives = 206/401 (51%), Gaps = 75/401 (18%) Query: 5 LQDAYIVAATRTPIGKAPRGMFKNVRPDDLLVRAIQSAIAQVPGLDPKLIEDAIIGCSFP 64 ++D +I A RTPIG+ G K+VR DDL +++ + + P +D K ++D I GC+ Sbjct: 1 MKDVFICDAIRTPIGRYG-GALKDVRADDLGAVPLRALMERNPQVDWKAVDDVIYGCANQ 59 Query: 65 EGAQGLNMARNAVLLAGLPNTIGGVTINRYCASGVTAIAMAADRIRVGQADVMIAGGAES 124 G N+AR ++LLAGLP + G TINR C SG+ A+ AA I+ G+ +MIAGG ES Sbjct: 60 AGEDNRNVARMSLLLAGLPQEVPGSTINRLCGSGMDALGTAARAIKSGETQLMIAGGVES 119 Query: 125 MSMVP-MMGFHPSV--NMGAFKDENIG---------MAYG---MGLTAERVAQQWKVSRE 169 M+ P +MG S A +D IG YG M TAE VA +KVSR Sbjct: 120 MTRAPFVMGKADSAFSRQAAIQDTTIGWRFVNALMKQKYGVDAMPETAENVAVDFKVSRA 179 Query: 170 AQDAFALAS------------------------------------HQKAIA----AQLAG 189 QD FA+ S H +A + A+L G Sbjct: 180 DQDQFAVRSQAKAAAAQANGTLAQEIVPVVIPQKKGDPITVTQDEHPRATSMEALARLKG 239 Query: 190 --PPPG---------------ALILVSEKILKQFNLTPLARFASFAVRGVPPEIMGIGPK 232 P G AL+L S + ++++ L P AR A GV P IMG+GP Sbjct: 240 VVKPDGSVTAGNASGVNDGACALLLASAEAVEKYQLKPRARILGMATAGVAPRIMGMGPA 299 Query: 233 EAIPVALRAAGITQDQLDWIELNEAFAAQALAVIQDLGL--DPTKVNPMGGAIALGHPLG 290 A L G+T DQ+D IELNEAFA+Q LAV+++LG+ D +VNP GGAIALGHPLG Sbjct: 300 PASKKVLAQLGMTIDQMDVIELNEAFASQGLAVLRELGVADDDARVNPNGGAIALGHPLG 359 Query: 291 ATGAIRAATTIHAMRRKNLKYGMVTMCVGTGMGAAGIFERM 331 +GA T + + R +Y + TMC+G G G A + ER+ Sbjct: 360 MSGARLVTTATYQLERTGGRYALCTMCIGVGQGIAMVIERV 400 >gi|300313421|ref|YP_003777513.1| 3-oxoadipyl-CoA thiolase [Herbaspirillum seropedicae SmR1] Length = 401 Score = 129 bits (324), Expect = 3e-31, Method: Compositional matrix adjust. Identities = 71/140 (50%), Positives = 87/140 (62%), Gaps = 2/140 (1%) Query: 194 ALILVSEKILKQFNLTPLARFASFAVRGVPPEIMGIGPKEAIPVALRAAGITQDQLDWIE 253 AL+L S K + L P AR A GV P IMG GP A+ L G+T Q+D IE Sbjct: 261 ALLLASPKAADLYRLKPRARVLGMATAGVAPRIMGFGPAPAVRKVLAQVGLTLAQMDVIE 320 Query: 254 LNEAFAAQALAVIQDLGL--DPTKVNPMGGAIALGHPLGATGAIRAATTIHAMRRKNLKY 311 LNEAFAAQ LAV++DLGL D VNP GGAIA+GHPLGA+GA T I+ + R +Y Sbjct: 321 LNEAFAAQGLAVMRDLGLPDDAAHVNPNGGAIAIGHPLGASGARLVTTAINQLERSGGRY 380 Query: 312 GMVTMCVGTGMGAAGIFERM 331 + TMC+G G G A + ER+ Sbjct: 381 ALCTMCIGVGQGIALVIERV 400 Score = 110 bits (274), Expect = 2e-25, Method: Compositional matrix adjust. Identities = 75/187 (40%), Positives = 99/187 (52%), Gaps = 16/187 (8%) Query: 7 DAYIVAATRTPIGKAPRGMFKNVRPDDLLVRAIQSAIAQVPGLDPKLIEDAIIGCSFPEG 66 +A I A RTP G+ G VR DDL I+S + + PG+D +ED + GC+ G Sbjct: 2 EALICDAIRTPFGRYG-GALGAVRADDLAAAPIRSLMERNPGVDWSRVEDILYGCANQAG 60 Query: 67 AQGLNMARNAVLLAGLPNTIGGVTINRYCASGVTAIAMAADRIRVGQADVMIAGGAESMS 126 N+AR A LLAGLP + G T+NR C S + A+ MAA I+ G+ +MIAGG ESM+ Sbjct: 61 EDNRNVARMAGLLAGLPIAVPGSTVNRLCGSSLDAVGMAARAIKSGEVQLMIAGGVESMT 120 Query: 127 MVP-MMGFHPS--VNMGAFKDENIGMAY------------GMGLTAERVAQQWKVSREAQ 171 P +MG S A D IG + M TAE VA ++++R Q Sbjct: 121 RAPFVMGKAESAFARSAAIFDTTIGWRFVNPLMKAQYGIDSMPETAENVATDFQINRADQ 180 Query: 172 DAFALAS 178 DAFAL S Sbjct: 181 DAFALRS 187 >gi|300309581|ref|YP_003773673.1| acetyl-CoA acetyltransferase [Herbaspirillum seropedicae SmR1] Length = 391 Score = 122 bits (306), Expect = 4e-29, Method: Compositional matrix adjust. Identities = 61/137 (44%), Positives = 90/137 (65%) Query: 194 ALILVSEKILKQFNLTPLARFASFAVRGVPPEIMGIGPKEAIPVALRAAGITQDQLDWIE 253 A+++ S K+ + L LA+ +++ G+ P IMG+GP A + L+ AG T LD +E Sbjct: 255 AVVVTSAKLAAELGLPVLAKIKAYSSAGLDPSIMGMGPVPASQLTLKKAGWTPQDLDLME 314 Query: 254 LNEAFAAQALAVIQDLGLDPTKVNPMGGAIALGHPLGATGAIRAATTIHAMRRKNLKYGM 313 +NEAFAAQA+AV + +G D +K+N GGAIALGHP+GA+G T +H M R++ K G+ Sbjct: 315 INEAFAAQAIAVNKQMGWDTSKINVNGGAIALGHPIGASGCRVLVTLLHEMVRRDAKKGL 374 Query: 314 VTMCVGTGMGAAGIFER 330 ++C+G GMG A ER Sbjct: 375 ASLCIGGGMGVALAVER 391 Score = 109 bits (273), Expect = 3e-25, Method: Compositional matrix adjust. Identities = 82/232 (35%), Positives = 120/232 (51%), Gaps = 17/232 (7%) Query: 5 LQDAYIVAATRTPIGKAPRGMFKNVRPDDLLVRAIQSAIAQVPGLDPKLIEDAIIGCSFP 64 + D IVAA RT IGK G + DL + I++ +A+ G+ P+ I + I+G Sbjct: 1 MDDVVIVAAQRTAIGKFG-GALSKIAAADLGAQVIKALLAKT-GIKPEAISEVILGQVLT 58 Query: 65 EGAQGLNMARNAVLLAGLPNTIGGVTINRYCASGVTAIAMAADRIRVGQADVMIAGGAES 124 G G N AR +V+ +GLP+ + + + C SG+ A+ +AA IR G A ++IAGG E+ Sbjct: 59 AGL-GQNPARQSVIKSGLPDMVPAFVVGKVCGSGLKAVQLAAQAIRCGDAQIVIAGGQEN 117 Query: 125 MSMVP--MMGFHPSVNMGAFKDE---------NIGMAYGMGLTAERVAQQWKVSREAQDA 173 MS P + MG K ++ Y MG+TAE VA+++++SRE QDA Sbjct: 118 MSASPHVLNNSRDGFRMGDAKLTDTMIVDGLWDVYNQYHMGITAENVAKKFEISREEQDA 177 Query: 174 FALASHQKAIAAQLAGPPPGALILVSEKILKQFNLTPLARFASFAVRGVPPE 225 FA AS KA AAQ AG ++ + K K+ T + F GV E Sbjct: 178 FAAASQNKAEAAQKAGKFKDEIVPIEIKGKKE---TVVFDTDEFVKHGVTAE 226 >gi|300310259|ref|YP_003774351.1| acetyl-CoA acetyltransferase [Herbaspirillum seropedicae SmR1] Length = 400 Score = 110 bits (274), Expect = 2e-25, Method: Compositional matrix adjust. Identities = 115/398 (28%), Positives = 173/398 (43%), Gaps = 85/398 (21%) Query: 10 IVAATRTPIGKAPRGMFKNVRPDDLLVRAIQSAI--AQVPGLDPKLIEDAIIGCSFPEGA 67 IV+A RT +G +G ++ L AI +A+ AQ+ IE +GC G Sbjct: 8 IVSAARTAMGSF-QGALADLPAPHLGAVAISAALQRAQLSAAQIAQIEQVWMGCVLQAGL 66 Query: 68 QGLNMARNAVLLAGLPNTIGGVTINRYCASGVTAIAMAADRIRVGQADVMIAGGAESMSM 127 G AR A L AGLP T+ T+N+ C S + A+ + D I G A +++AGG ESMS Sbjct: 67 -GQAPARQAALRAGLPQTVACATLNKVCGSAMQAVMLGHDSILAGSASMVVAGGMESMSN 125 Query: 128 VPMMGFHPSVNMG-------------------AFKDENIGMAYGMGLTAERVAQQWKVSR 168 P + P G A+ D+ G MG AE A ++ +R Sbjct: 126 APYL--LPKARRGYRLGHDRLLDHMFLDGLEDAYSDQYRGRL--MGTFAEDCASEFGFTR 181 Query: 169 EAQDAFALASHQKA-------------IAAQLAGPPPGALILVSEKILKQFNLTPLARFA 215 QDAFA+ S +A + +AG G++++ ++ NL +A Sbjct: 182 AQQDAFAVQSTLRARQAISGGGFDWEVVPVDVAG-KKGSVVISQDEGPMAVNLERIATLK 240 Query: 216 -SFAV-----------------------------RGVPP--EIMG----IGPKEAIPVA- 238 +F+V +G+ P I+G G E P A Sbjct: 241 PAFSVDGTVTAANSSSISDGAAALLLMRESAALKQGLTPLARILGHASYAGAPEKFPTAP 300 Query: 239 ---LR----AAGITQDQLDWIELNEAFAAQALAVIQDLGLDPTKVNPMGGAIALGHPLGA 291 LR G+ D E+NEAFA +A ++L +D K+N GGA ALGHP+GA Sbjct: 301 IGVLRKIFAKTGLDAASTDLFEINEAFAVVPMAASRELDIDMEKINVHGGACALGHPIGA 360 Query: 292 TGAIRAATTIHAMRRKNLKYGMVTMCVGTGMGAAGIFE 329 +GA + + A++ + LK G+ ++C+G G A E Sbjct: 361 SGARIIVSLLGALKARGLKTGIASLCIGGGEATAIALE 398 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.135 0.393 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 904,476 Number of extensions: 35141 Number of successful extensions: 183 Number of sequences better than 1.0e-08: 6 Number of HSP's gapped: 175 Number of HSP's successfully gapped: 12 Length of query: 331 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 245 Effective length of database: 1,212,660 Effective search space: 297101700 Effective search space used: 297101700 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 131 (55.1 bits)