BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0004.0129.p (254 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309599|ref|YP_003773691.1| enoyl-CoA hydratase [Herbaspiri... 419 e-118 gi|300313417|ref|YP_003777509.1| enoyl-CoA hydratase II protein ... 127 7e-31 gi|300313068|ref|YP_003777160.1| enoyl-CoA hydratase [Herbaspiri... 115 4e-27 gi|300313175|ref|YP_003777267.1| enoyl-CoA hydratase [Herbaspiri... 105 3e-24 gi|300313422|ref|YP_003777514.1| enoyl-CoA hydratase/isomerase [... 102 4e-23 >gi|300309599|ref|YP_003773691.1| enoyl-CoA hydratase [Herbaspirillum seropedicae SmR1] Length = 256 Score = 419 bits (1076), Expect = e-118, Method: Compositional matrix adjust. Identities = 212/254 (83%), Positives = 237/254 (93%) Query: 1 MEIELTKSNGVLTIAFNREEKKNAITAAMYQQLADALKDAETDNAVRAILFTGKPQIFTA 60 M+I+L+K++GVLTI F+RE+KKNAITAAMYQQLADALK+AE+D+AVRAILFTGKP+ F+A Sbjct: 3 MDIQLSKADGVLTIHFDREDKKNAITAAMYQQLADALKEAESDSAVRAILFTGKPKAFSA 62 Query: 61 GNDLEDFLKNPPSSKDAPVFQFLWEISHVSKPIVAAVSGAAVGIGTTLLLHCDLVYAADN 120 GNDLEDFLKNPP ++DAPVFQFLW+ISH SKPIVAAVSG AVGIGTTLLLHCDLVYAADN Sbjct: 63 GNDLEDFLKNPPKTQDAPVFQFLWQISHASKPIVAAVSGVAVGIGTTLLLHCDLVYAADN 122 Query: 121 AKFSMPFTQLGLCPEAASSLLLPHIAGYQRAAEKLLLGEAFDAAEAHAMGIVNRVLTVEE 180 AKFSMPFTQLGLCPEAASSLLLPHIAGYQRAAEKLLLGEAF A EA MG VNRV+ EE Sbjct: 123 AKFSMPFTQLGLCPEAASSLLLPHIAGYQRAAEKLLLGEAFGADEACQMGFVNRVMAQEE 182 Query: 181 LQPFAQKQAAKLAALPASSVRITKQLMKKNRVAATEAQMAAEIEHFAKMLTAPEAKEAFT 240 L P+AQ+QAAKLAALPA+S+R+TKQLMKKNRV ATE QM AEIEHF++MLTAPEAKEAFT Sbjct: 183 LLPYAQQQAAKLAALPAASIRVTKQLMKKNRVTATEQQMVAEIEHFSRMLTAPEAKEAFT 242 Query: 241 AFFEKRRPDFSKFA 254 AFFEKR+PDF +FA Sbjct: 243 AFFEKRKPDFKQFA 256 >gi|300313417|ref|YP_003777509.1| enoyl-CoA hydratase II protein [Herbaspirillum seropedicae SmR1] Length = 263 Score = 127 bits (320), Expect = 7e-31, Method: Compositional matrix adjust. Identities = 81/256 (31%), Positives = 121/256 (47%), Gaps = 7/256 (2%) Query: 3 IELTKSNGVLTIAFNREEKKNAITAAMYQQLADALKDAETDNAVRAILFTGKPQIFTAGN 62 I L +G+ I NR +K N+ T AM+ +L DA+ + D +VR +L TG + F AG Sbjct: 6 ILLENRDGIAVITLNRPDKLNSFTVAMHLELRDAIATLQADPSVRVLLLTGAGRGFCAGQ 65 Query: 63 DLEDFLKNPPS-------SKDAPVFQFLWEISHVSKPIVAAVSGAAVGIGTTLLLHCDLV 115 DL D P S D + + + P++ AV+G A G G L L CD+V Sbjct: 66 DLGDRAVKPGDDGVDLGESIDKYYGPLVKSLRALPFPVICAVNGVAAGAGANLPLACDIV 125 Query: 116 YAADNAKFSMPFTQLGLCPEAASSLLLPHIAGYQRAAEKLLLGEAFDAAEAHAMGIVNRV 175 AA +A F F +LGL P+ + LP + G RA LLGE A +A G++ + Sbjct: 126 LAARSASFVEVFCKLGLIPDTGGTYFLPRLVGTARAMGMALLGEKISAEQAERWGLIWKC 185 Query: 176 LTVEELQPFAQKQAAKLAALPASSVRITKQLMKKNRVAATEAQMAAEIEHFAKMLTAPEA 235 + ++L A AA A P + TK + + Q+ E + + + + Sbjct: 186 VDDDQLMEEAHAMAAHFARAPTKGLAATKATLYASPAHTLPEQLDLERDTMRALGRSRDY 245 Query: 236 KEAFTAFFEKRRPDFS 251 +E TAF EKR P F+ Sbjct: 246 REGVTAFLEKRAPQFT 261 >gi|300313068|ref|YP_003777160.1| enoyl-CoA hydratase [Herbaspirillum seropedicae SmR1] Length = 263 Score = 115 bits (287), Expect = 4e-27, Method: Compositional matrix adjust. Identities = 76/256 (29%), Positives = 128/256 (50%), Gaps = 11/256 (4%) Query: 3 IELTKSNGVLTIAFNREEKKNAITAAMYQQLADALKDAETDNAVRAILFTGKPQIFTAGN 62 +E G+ T+ NR + NA++ M L L D ++D+ VR ++ + F AG+ Sbjct: 9 LEREDQQGITTLTLNRPQHFNALSEEMLTALQAQLDDIKSDSTVRCVILAANGKAFCAGH 68 Query: 63 DLEDFLKNPPSSKDAPVFQ----FLWEISHVSKPIVAAVSGAAVGIGTTLLLHCDLVYAA 118 DL P + +F + + + P++A V G A G L+ CDL AA Sbjct: 69 DLRQMQATPQQTYYEALFHRCSHLMQSLRELPVPVIAKVQGLATAAGCQLVATCDLAIAA 128 Query: 119 DNAKFSMPFTQLGL-C--PEAASSLLLPHIAGYQRAAEKLLLGEAFDAAEAHAMGIVNRV 175 ++A+F++ +GL C P A + LP +RA E L+ G+ DAA A A G++N+ Sbjct: 129 ESARFAVSGINVGLFCSTPAVALTRNLP----IKRAFEMLVTGKFIDAATAAAWGLINQA 184 Query: 176 LTVEELQPFAQKQAAKLAALPASSVRITKQLMKKNRVAATEAQMAAEIEHFAKMLTAPEA 235 + +EL Q A ++ + PA S+ K ++ + ++ A A E A + PEA Sbjct: 185 VPEQELDSATQALAREICSKPAISIARGKAMVYRQQMMALPDAYAHAAEVMACNIMDPEA 244 Query: 236 KEAFTAFFEKRRPDFS 251 +E TAF +KR+P+++ Sbjct: 245 QEGITAFLDKRKPNWA 260 >gi|300313175|ref|YP_003777267.1| enoyl-CoA hydratase [Herbaspirillum seropedicae SmR1] Length = 258 Score = 105 bits (263), Expect = 3e-24, Method: Compositional matrix adjust. Identities = 74/257 (28%), Positives = 117/257 (45%), Gaps = 16/257 (6%) Query: 3 IELTKSNGVLTIAFNREEKKNAITAAMYQQLADALKDAETDNAVRAILFTGKPQIFTAGN 62 I++ V I +R + NA++ + +L +AL + + I+ TG + F AG Sbjct: 6 IQVETHEKVGLIRLHRPKALNALSDGLMTELGEALLAFDAQEEIGCIIITGSEKAFAAGA 65 Query: 63 DLE--------DFLKNPPSSKDAPVFQFLWE-ISHVSKPIVAAVSGAAVGIGTTLLLHCD 113 D+ D K +++ WE + + KP++AAV+G A+G G L + CD Sbjct: 66 DISAMAGYDYMDVFKGEFITRN-------WETLRRIRKPVIAAVAGYALGGGCELAMMCD 118 Query: 114 LVYAADNAKFSMPFTQLGLCPEAASSLLLPHIAGYQRAAEKLLLGEAFDAAEAHAMGIVN 173 + AADNAKF P +LG+ P A + LP +A + L G DA EA G+V+ Sbjct: 119 FIIAADNAKFGQPEIKLGIVPGAGGTQRLPRAVSKAKAMDLALTGRMMDAEEAERAGLVS 178 Query: 174 RVLTVEELQPFAQKQAAKLAALPASSVRITKQLMKKNRVAATEAQMAAEIEHFAKMLTAP 233 R++ ++L A A +A +P + K + + M E F Sbjct: 179 RIVAADKLLEEAMAAAIIIAEMPRQVAMMVKDSVNRAYETTLSEGMKYERALFYSCFATE 238 Query: 234 EAKEAFTAFFEKRRPDF 250 + KE AF EKR+P F Sbjct: 239 DQKEGMKAFLEKRQPVF 255 >gi|300313422|ref|YP_003777514.1| enoyl-CoA hydratase/isomerase [Herbaspirillum seropedicae SmR1] Length = 262 Score = 102 bits (253), Expect = 4e-23, Method: Compositional matrix adjust. Identities = 77/246 (31%), Positives = 117/246 (47%), Gaps = 9/246 (3%) Query: 11 VLTIAFNREEKKNAITAAMYQQLADALKDAETDNAVRAILFTGKPQIFTAGNDLEDFLKN 70 VL + NR +NA+ +++ AL AE D++VR ++ +G + F AG DL + + Sbjct: 19 VLHLTLNRPALRNALRNQSLREIVAALARAEEDDSVRVVVISGNEKAFAAGADLNEMIHK 78 Query: 71 PPSSKDAPVFQFLWE-ISHVSKPIVAAVSGAAVGIGTTLLLHCDLVYAADNAKFSMPFTQ 129 + V W I+ KPI+AAV+G A+G G LL+H D+ AA AK P Sbjct: 79 DAIATQLDVRAQYWRAIARFPKPILAAVNGYALGAGCELLMHADIAIAARGAKIGQPEIN 138 Query: 130 LGLCPEAASSLLLPHIAGYQRAAEKLLLGEAFDAAEAHAMGIVNRVL----TVEELQPFA 185 +G P A + L G A + +L GE A +A G+V V+ T+E A Sbjct: 139 VGTLPGAGGTQRLIRTVGKPLAMKMVLSGEFISADQALQAGLVAEVVDDDATLERTLALA 198 Query: 186 QKQAAKLAALPASSVRITKQLMKKNRVAATEAQMAAEIEHFAKMLTAPEAKEAFTAFFEK 245 A K +VR+ K+ M ++ EA + E + F+ M + + +E AF EK Sbjct: 199 HSIAQK----SPLAVRLAKEAMLQSFELGLEAGLLFERKSFSLMAASADRQEGIAAFQEK 254 Query: 246 RRPDFS 251 R FS Sbjct: 255 RAAVFS 260 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.130 0.361 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 596,809 Number of extensions: 18960 Number of successful extensions: 85 Number of sequences better than 1.0e-08: 9 Number of HSP's gapped: 79 Number of HSP's successfully gapped: 9 Length of query: 254 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 171 Effective length of database: 1,226,865 Effective search space: 209793915 Effective search space used: 209793915 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 129 (54.3 bits)