BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0004.0128.p (179 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310258|ref|YP_003774350.1| 3-hydroxyacyl-CoA (short-chain)... 292 1e-80 gi|300310506|ref|YP_003774598.1| type II 3-hydroxyacyl-CoA dehyd... 240 5e-65 gi|300313296|ref|YP_003777388.1| type II 3-hydroxyacyl-CoA dehyd... 202 2e-53 gi|300312302|ref|YP_003776394.1| acetoacetyl-CoA reductase [Herb... 82 2e-17 gi|300313110|ref|YP_003777202.1| 3-oxoacyl-(acyl-carrier-protein... 82 2e-17 >gi|300310258|ref|YP_003774350.1| 3-hydroxyacyl-CoA (short-chain) dehydrogenase type II oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 254 Score = 292 bits (748), Expect = 1e-80, Method: Compositional matrix adjust. Identities = 142/179 (79%), Positives = 159/179 (88%) Query: 1 MGSVVGLINCAGIAPAEKVLGKEGVHRLESFVRAVNINLIGTFNMARLAVEAISENEPSE 60 MG+VVGL+NCAGIAP EK+LGK GVHRL+SF+RA+NINL+G+FNMARLA + IS Sbjct: 76 MGAVVGLVNCAGIAPGEKLLGKGGVHRLDSFMRALNINLVGSFNMARLAAQHISAGPADA 135 Query: 61 NGERGVIINTASIAAFDGQVGQAAYAASKGGVVAMTLPLARELSRYGIRVMTIAPGIIET 120 GERGVII+TASIAAFDGQ+GQ AYAASKGGVV+MTLP+AREL+R+GIRV+TIAPGIIET Sbjct: 136 TGERGVIIHTASIAAFDGQMGQVAYAASKGGVVSMTLPMARELARHGIRVVTIAPGIIET 195 Query: 121 PMMLDMPAEVLSSLGKMVPFPPRLGKPSEFASLVEHILENRYLNGEVIRMDGALRMGAK 179 PMML MP EVL SL + VPFPPRLGKPSEFA LV HILENRYLNGEVIRMDGALRM AK Sbjct: 196 PMMLGMPEEVLESLSRNVPFPPRLGKPSEFAGLVMHILENRYLNGEVIRMDGALRMSAK 254 >gi|300310506|ref|YP_003774598.1| type II 3-hydroxyacyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 252 Score = 240 bits (612), Expect = 5e-65, Method: Compositional matrix adjust. Identities = 119/179 (66%), Positives = 146/179 (81%) Query: 1 MGSVVGLINCAGIAPAEKVLGKEGVHRLESFVRAVNINLIGTFNMARLAVEAISENEPSE 60 +GS+ GL+NCAGIAPAEK++GK G H + F++ +NINL+G+FNMAR A EA++ + E Sbjct: 74 LGSLRGLVNCAGIAPAEKLVGKSGPHTMALFMKTLNINLVGSFNMARFAAEAMARSAAVE 133 Query: 61 NGERGVIINTASIAAFDGQVGQAAYAASKGGVVAMTLPLARELSRYGIRVMTIAPGIIET 120 GERGVII+TAS+AA+DGQ+GQ AYAASKG +VAMTLP+ARELSR GIRVMTIAPGI ET Sbjct: 134 EGERGVIIHTASVAAYDGQIGQTAYAASKGAIVAMTLPMARELSRSGIRVMTIAPGIFET 193 Query: 121 PMMLDMPAEVLSSLGKMVPFPPRLGKPSEFASLVEHILENRYLNGEVIRMDGALRMGAK 179 PMML MP EV SLG+ VPFPPRLG+ +E+A L I+EN LNGE IR+DGA+RM K Sbjct: 194 PMMLAMPQEVRDSLGQAVPFPPRLGRAAEYAQLARTIIENVMLNGETIRLDGAIRMQPK 252 >gi|300313296|ref|YP_003777388.1| type II 3-hydroxyacyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 254 Score = 202 bits (513), Expect = 2e-53, Method: Compositional matrix adjust. Identities = 105/171 (61%), Positives = 129/171 (75%), Gaps = 1/171 (0%) Query: 7 LINCAGIAPAEKVLGKEGVHR-LESFVRAVNINLIGTFNMARLAVEAISENEPSENGERG 65 LINCAGI A++++GK+G LE F R VN+NLIGTFN+ RLA ++ E GERG Sbjct: 81 LINCAGIGGAKRMVGKDGKPMPLEDFSRIVNVNLIGTFNVIRLAAARMAAAEALAEGERG 140 Query: 66 VIINTASIAAFDGQVGQAAYAASKGGVVAMTLPLARELSRYGIRVMTIAPGIIETPMMLD 125 VI+ TAS+AAFDGQVGQA YAASKGG+ AMTLPLAR+L++YGIRV+TIAPG+ TP++ Sbjct: 141 VIVATASVAAFDGQVGQAGYAASKGGITAMTLPLARDLAQYGIRVVTIAPGLFLTPLLYK 200 Query: 126 MPAEVLSSLGKMVPFPPRLGKPSEFASLVEHILENRYLNGEVIRMDGALRM 176 +P EV SL +PFP RLGK E+A L HI N LNGEVIR+DGALR+ Sbjct: 201 LPEEVQQSLASSIPFPKRLGKAEEYAQLALHIATNLSLNGEVIRLDGALRL 251 >gi|300312302|ref|YP_003776394.1| acetoacetyl-CoA reductase [Herbaspirillum seropedicae SmR1] Length = 246 Score = 82.4 bits (202), Expect = 2e-17, Method: Compositional matrix adjust. Identities = 59/182 (32%), Positives = 91/182 (50%), Gaps = 19/182 (10%) Query: 1 MGSVVGLINCAGIAPAEKVLGKEGVHRL---ESFVRAVNINLIGTFNMARLAVEAISENE 57 +G V L+N AGI ++G R + + ++ NL FN+ + +E + E Sbjct: 79 IGEVDVLVNNAGIT-------RDGQFRKMSKDDWDAVIDTNLNSLFNVTKQVIEGMCERG 131 Query: 58 PSENGERGVIINTASIAAFDGQVGQAAYAASKGGVVAMTLPLARELSRYGIRVMTIAPGI 117 G IIN +S+ GQ GQ Y+ +K G+ T+ LA+E++ G+ V T++PG Sbjct: 132 F------GRIINISSVNGQKGQFGQTNYSTAKAGIHGFTMALAQEVATKGVTVNTVSPGY 185 Query: 118 IETPMMLDMPAEVLSSLGKMVPFPPRLGKPSEFASLVEHIL--ENRYLNGEVIRMDGALR 175 I T M+ + EVL + +P RLG+P E AS+V I E Y G ++G L Sbjct: 186 IGTDMVRAIRPEVLEKIVSGIPV-KRLGEPEEIASIVAWIASSEGGYATGSDFSLNGGLY 244 Query: 176 MG 177 MG Sbjct: 245 MG 246 >gi|300313110|ref|YP_003777202.1| 3-oxoacyl-(acyl-carrier-protein) reductase [Herbaspirillum seropedicae SmR1] Length = 256 Score = 82.0 bits (201), Expect = 2e-17, Method: Compositional matrix adjust. Identities = 59/178 (33%), Positives = 93/178 (52%), Gaps = 22/178 (12%) Query: 7 LINCAGIAPAEKVLGKEGVHRLESFVRAVNINLIGTF----NMARLAVEAISENEPSENG 62 L+N AG+A L + LE++++ +N+N+ G + ARL V+ Sbjct: 89 LVNNAGVARTYSFLD----YPLENWLQTMNVNVTGVLLAGQHAARLMVKQ---------- 134 Query: 63 ERGVIINTASIAAFDGQVGQAAYAASKGGVVAMTLPLARELSRYGIRVMTIAPGIIETPM 122 + G I+N +SI+ G+ AY SK V+ +T +A EL++YGI V ++APG ++TPM Sbjct: 135 QWGRIVNISSISGIRAGAGRTAYGTSKAAVIGLTRQMAIELAQYGITVNSVAPGPVDTPM 194 Query: 123 MLDMPAE-VLSSLGKMVPFPPRLGKPSEFASLVEHIL--ENRYLNGEVIRMDGALRMG 177 M +E S ++VP R G E AS V ++ E+ Y+ G VI +DG G Sbjct: 195 TQAMHSEQTRESYYRLVPM-RRYGTTDEIASAVCYLAAEESAYITGHVIPVDGGFVAG 251 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.136 0.379 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 572,255 Number of extensions: 23606 Number of successful extensions: 141 Number of sequences better than 1.0e-08: 23 Number of HSP's gapped: 140 Number of HSP's successfully gapped: 23 Length of query: 179 Length of database: 1,619,870 Length adjustment: 79 Effective length of query: 100 Effective length of database: 1,245,805 Effective search space: 124580500 Effective search space used: 124580500 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 127 (53.5 bits)