BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0004.0099.p (426 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310307|ref|YP_003774399.1| HD-GYP domain metal-dependent p... 501 e-143 gi|300310279|ref|YP_003774371.1| HD-GYP domain-containing protei... 466 e-132 gi|300313282|ref|YP_003777374.1| HD-GYP domain-containing protei... 423 e-119 gi|300311984|ref|YP_003776076.1| HD-GYP domain-containing protei... 316 3e-87 gi|300314021|ref|YP_003778113.1| HD-GYP domain-containing protei... 315 7e-87 >gi|300310307|ref|YP_003774399.1| HD-GYP domain metal-dependent phosphohydrolase [Herbaspirillum seropedicae SmR1] Length = 406 Score = 501 bits (1289), Expect = e-143, Method: Compositional matrix adjust. Identities = 238/420 (56%), Positives = 308/420 (73%), Gaps = 19/420 (4%) Query: 3 LLKKIKVDELELGMYFCGFEAAWLDHPFWKNRFLLKDTAHLQQARASGIAYCWIDVGQGK 62 +LKKIKV++L LGMYFCGFEAAWLDHPFWKN+FLLK+ + L+QA ASGIAYCWID +G Sbjct: 1 MLKKIKVEDLVLGMYFCGFEAAWLDHPFWKNKFLLKNDSELRQAHASGIAYCWIDTERGL 60 Query: 63 DVAAAEQDADVAAE-SEAESGVESGVESEGAETALSLRDELPVAVGADPFFEELEQAQRL 121 DVA E+ AD AA E E E P A P L A L Sbjct: 61 DVAQEEEAADSAAPLQETEPSPE------------------PEHAAAPPIDAVLLDAMAL 102 Query: 122 CETAKDATKAMFADIRMGRVIDMERCLDLVDQVTASILRNSAAMTSIVRLKLADDYTYMH 181 ETAK ATKAMF++ RMG+V+DMERC+ +V+++ S++++ S++RLKLAD+YTY+H Sbjct: 103 RETAKKATKAMFSEARMGKVLDMERCVSVVEEIAGSVIKSPNGALSVLRLKLADEYTYLH 162 Query: 182 SVAVCTLMVALGRTIGMDEPACKEAGLSGLLHDVGKAFIPVPILHKPGGLTPAEIDIVRR 241 SVAVC LMVALGRT+G+DE AC+EAGL G LHDVGKAFIP +L+KPG L+ E +++ Sbjct: 163 SVAVCALMVALGRTLGLDETACREAGLGGFLHDVGKAFIPHELLNKPGKLSDEEFSQIKK 222 Query: 242 HPVLGYNHLIKNSNIPPYAVEVCLHHHEKINGTGYPDGLSGAGISLLSRMASICDVYDAV 301 HP LGY +L ++ N+P YA +VCLHHHEKI+G GYP+GL I+L SRMA+ICDVYDA+ Sbjct: 223 HPELGYKYLSQDPNVPDYARDVCLHHHEKIDGNGYPEGLRAGAITLYSRMAAICDVYDAL 282 Query: 302 TSDRPYKKGWDPAESLTAMASWSGHFDKTLLSAFVKTLGIYPIGSLLKMESGRLGLVIRQ 361 TSDRPYK+ WDPAE+++AMASW+GHFD+ + AFVKTLGIYP+GS++K+ESGR G VIRQ Sbjct: 283 TSDRPYKRAWDPAEAISAMASWTGHFDRAMFLAFVKTLGIYPVGSIVKLESGRSGFVIRQ 342 Query: 362 SAAGLTKPVVKAFRYDSNGDIIDSEIIDLSDPAASDSIRQRGGEEWLKYNGLNKLWMQPT 421 +A LT+PV+K F YD + + D+EI+DL +D+I RGGEEW + + L+ + + T Sbjct: 343 NAQDLTRPVLKVFHYDDSMTVTDTEILDLLANPEADTIVARGGEEWARLSHLDAMQLLST 402 >gi|300310279|ref|YP_003774371.1| HD-GYP domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 408 Score = 466 bits (1198), Expect = e-132, Method: Compositional matrix adjust. Identities = 222/413 (53%), Positives = 291/413 (70%), Gaps = 25/413 (6%) Query: 1 MSLLKKIKVDELELGMYFCGFEAAWLDHPFWKNRFLLKDTAHLQQARASGIAYCWIDVGQ 60 M+ LKKI+V +L +GMYF GF+ AW+D PFW+ FL++ + + +ARA+G YCWID Q Sbjct: 1 MTRLKKIEVGQLSVGMYFSGFDGAWIDTPFWRTSFLVRKASEIARARAAGYTYCWIDTDQ 60 Query: 61 GKDVAAAEQDADVAAESEAESGVESGVESEGAETALSLRDELPVAVGADPFFEELEQAQR 120 G D+A AE AD E+E P A G+ EL+ A R Sbjct: 61 GIDLAGAE--ADRLPETEE-----------------------PEAAGSSSSRSELDDALR 95 Query: 121 LCETAKDATKAMFADIRMGRVIDMERCLDLVDQVTASILRNSAAMTSIVRLKLADDYTYM 180 L E AK T+ MF R+G+ +D+E C LV+++ S+ R+S A+ SI+RLK D+YTY+ Sbjct: 96 LYENAKQTTQQMFKQARLGKTLDVENCKSLVEEIATSVARDSNALLSIIRLKRVDEYTYL 155 Query: 181 HSVAVCTLMVALGRTIGMDEPACKEAGLSGLLHDVGKAFIPVPILHKPGGLTPAEIDIVR 240 HS+AVCTLMVAL RT+GMD+ ACKEAGL+G LHDVGKAFIP+ IL+KP L+ E I++ Sbjct: 156 HSIAVCTLMVALARTLGMDDIACKEAGLAGYLHDVGKAFIPLEILNKPDRLSEQEYKIIQ 215 Query: 241 RHPVLGYNHLIKNSNIPPYAVEVCLHHHEKINGTGYPDGLSGAGISLLSRMASICDVYDA 300 RHP LGY++L S +P Y ++VCLHHHEKI+G+GYP G IS+ SRMA++CDVYDA Sbjct: 216 RHPALGYDYLKGFSGVPDYVLDVCLHHHEKIDGSGYPSRHQGNQISVYSRMAAVCDVYDA 275 Query: 301 VTSDRPYKKGWDPAESLTAMASWSGHFDKTLLSAFVKTLGIYPIGSLLKMESGRLGLVIR 360 VTS+RPYK GWDPAESL+ MASW GHFD+ + AFVK LGIYPIGS++K++SGR LVIR Sbjct: 276 VTSERPYKSGWDPAESLSRMASWQGHFDRPIFLAFVKALGIYPIGSIVKLQSGRTALVIR 335 Query: 361 QSAAGLTKPVVKAFRYDSNGDIIDSEIIDLSDPAASDSIRQRGGEEWLKYNGL 413 Q+ LTKPVVKAF + ++++SEIIDLS P +D+I +R G++WLK NGL Sbjct: 336 QNHVDLTKPVVKAFVTTPSIEVVESEIIDLSAPGQTDAIAERSGQDWLKSNGL 388 >gi|300313282|ref|YP_003777374.1| HD-GYP domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 410 Score = 423 bits (1087), Expect = e-119, Method: Compositional matrix adjust. Identities = 206/411 (50%), Positives = 284/411 (69%), Gaps = 28/411 (6%) Query: 3 LLKKIKVDELELGMYFCGFEAAWLDHPFWKNRFLLKDTAHLQQARASGIAYCWIDVGQGK 62 +LKKIKV +L LGMYFCGFEA+WL HPFWK RFLL + LQQARASGIAYCWIDV +G+ Sbjct: 1 MLKKIKVGDLVLGMYFCGFEASWLAHPFWKTRFLLSRESDLQQARASGIAYCWIDVSRGR 60 Query: 63 DVAAAEQDADVAAES----EAESGVESGVESEGAETALSLRDELPVAVGADPFFEELEQA 118 DVA A AA + + + +G +++ A +A++LR Sbjct: 61 DVAGAAPAKAEAAAAPASPDQSAATHAGADADRA-SAVALR------------------- 100 Query: 119 QRLCETAKDATKAMFADIRMGRVIDMERCLDLVDQVTASILRNSAAMTSIVRLKLADDYT 178 E A++ T+ MF RMG+++++ +C+ LV+++ S+LR+ + S++RLKL D+YT Sbjct: 101 ----EHAREVTRQMFNAARMGKMVELPQCVALVEEIACSVLRDPNGLLSVLRLKLNDEYT 156 Query: 179 YMHSVAVCTLMVALGRTIGMDEPACKEAGLSGLLHDVGKAFIPVPILHKPGGLTPAEIDI 238 Y+HSV+VC L++ LGRT+G+ E AC+EAGLSG LHDVGKAFI IL+KPG LT E + Sbjct: 157 YLHSVSVCALVIGLGRTLGLSEAACREAGLSGYLHDVGKAFIDDEILNKPGKLTAEEFAL 216 Query: 239 VRRHPVLGYNHLIKNSNIPPYAVEVCLHHHEKINGTGYPDGLSGAGISLLSRMASICDVY 298 ++RHP LGY +LI+ ++P YA +VC HHHE+++G GYPD L I+L +RM ++CDVY Sbjct: 217 IKRHPELGYQYLIQTPDMPAYASDVCRHHHERLDGRGYPDALPAESITLYARMTAVCDVY 276 Query: 299 DAVTSDRPYKKGWDPAESLTAMASWSGHFDKTLLSAFVKTLGIYPIGSLLKMESGRLGLV 358 DA+TS+RPYK GWDPAE+++ MA W GHFD+ LL AFVKTLGIYP+GSL+++ESG G+V Sbjct: 277 DALTSERPYKHGWDPAEAISQMARWEGHFDRRLLLAFVKTLGIYPVGSLVRLESGVSGVV 336 Query: 359 IRQSAAGLTKPVVKAFRYDSNGDIIDSEIIDLSDPAASDSIRQRGGEEWLK 409 IRQ+ A LT+PV+K + ++DL +D+I RG EEW + Sbjct: 337 IRQNPADLTRPVLKVLHLQGPHETTIGGVLDLMAWPRTDTIIGRGDEEWTR 387 >gi|300311984|ref|YP_003776076.1| HD-GYP domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 406 Score = 316 bits (809), Expect = 3e-87, Method: Compositional matrix adjust. Identities = 168/393 (42%), Positives = 235/393 (59%), Gaps = 13/393 (3%) Query: 3 LLKKIKVDELELGMYFCGFEAAWLDHPFWKNRFLLKDTAHLQQARASGIAYCWIDVGQGK 62 +LK I +L +GMY F AW P W+ FLL+ L +AS I ID+ +G Sbjct: 1 MLKFITPKQLRIGMYIQSFSGAWRRPPAWRKPFLLEKDKDLLALQASPIREIVIDIAKGA 60 Query: 63 DVAAAEQDADVAAESEAESGVESGVESEGAETALSLRDELPVAVGADPFFEELEQAQRLC 122 DV E +E +GVES + G + + LP A E E+A R+ Sbjct: 61 DVQTDE--------AEQGNGVESSL---GKNAEIEVAQALPEAGKRTDIQAERERAARII 109 Query: 123 ETAKDATKAMFADIRMGRVIDMERCLDLVDQVTASILRNSAAMTSIVRLKLADDYTYMHS 182 +++ MF+ RMG+ ID++ DLV ++ AS+ RN+ AM S+VRLK AD+YTYMHS Sbjct: 110 SSSEKTVLTMFSQARMGKTIDVKEASDLVREINASVSRNADAMISLVRLKTADNYTYMHS 169 Query: 183 VAVCTLMVALGRTIGMDEPACKEAGLSGLLHDVGKAFIPVPILHKPGGLTPAEIDIVRRH 242 VAVC +M++L +G+ ++AG++GLLHD+GK +P IL+KP LT E V+ H Sbjct: 170 VAVCAMMISLADQLGLSAQERQQAGMAGLLHDIGKMTVPPSILNKPSKLTEEEFVSVQSH 229 Query: 243 PVLGYNHLIKNSNIPPYAVEVCLHHHEKINGTGYPDGLSGAGISLLSRMASICDVYDAVT 302 P+ G+ L + I ++V LHHHEK++GTGYP LSG IS+ +RM +ICDVYDA+T Sbjct: 230 PLAGHRLLQELPGIGDAPLDVSLHHHEKMDGTGYPYKLSGRDISMTARMGAICDVYDAIT 289 Query: 303 SDRPYKKGWDPAESLTAMASWSGHFDKTLLSAFVKTLGIYPIGSLLKMESGRLGLVIRQS 362 SDRPYK GWDP+ S+ MA GHFD +L AFVK +GIYP+GS + M++G L +V+ Q Sbjct: 290 SDRPYKSGWDPSRSIQHMAQSKGHFDFDVLQAFVKAVGIYPVGSCVLMQTGHLAMVLEQR 349 Query: 363 AAGLTKPVVKAFRYDSNGDI-IDSEIIDLSDPA 394 L P+V+ F Y +N I + +IDL A Sbjct: 350 PDSLLAPLVRIF-YSTNKKIAVTPTLIDLRRSA 381 >gi|300314021|ref|YP_003778113.1| HD-GYP domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 401 Score = 315 bits (806), Expect = 7e-87, Method: Compositional matrix adjust. Identities = 166/397 (41%), Positives = 237/397 (59%), Gaps = 16/397 (4%) Query: 3 LLKKIKVDELELGMYFCGFEAAWLDHPFWKNRFLLKDTAHLQQARASGIAYCWIDVGQGK 62 +LK I +D+L LGMY W+ HPF + F ++ LQ R I I+ +G+ Sbjct: 1 MLKSIALDQLRLGMYIHSIPGGWISHPFRRKSFKVETLEDLQTLRDCPIDAITINTAKGR 60 Query: 63 DVAAAEQDADVAAESEAESGVESGVESEGAETALSLRDELPVAVGADPFFEELEQAQRLC 122 DV E + D A + + A R + + E E+AQR+ Sbjct: 61 DVTREELEEDDADQDAPAAPPADAANRNPAS-----RTDTAI---------ERERAQRII 106 Query: 123 ETAKDATKAMFADIRMGRVIDMERCLDLVDQVTASILRNSAAMTSIVRLKLADDYTYMHS 182 ++K MF++ RMG+ ID++ +LV ++TAS+ RN+ A+ S+ RLK DDYTY+HS Sbjct: 107 SSSKSTVLHMFSEARMGKAIDVKDAAELVTEITASVSRNADALISLARLKTTDDYTYLHS 166 Query: 183 VAVCTLMVALGRTIGMDEPACKEAGLSGLLHDVGKAFIPVPILHKPGGLTPAEIDIVRRH 242 VAVC +M++L +GM K+AG++GLLHDVGK +P+ IL+KP LT E VR H Sbjct: 167 VAVCAMMISLANQLGMTPQQTKQAGMAGLLHDVGKMAVPLDILNKPAKLTEEEFASVRMH 226 Query: 243 PVLGYNHLIKNSNIPPYAVEVCLHHHEKINGTGYPDGLSGAGISLLSRMASICDVYDAVT 302 V G+ L + I A++V LHHHEKI+G+GYP L IS++++M ++CDVYDA+T Sbjct: 227 TVQGHGILQQIEGIGEVALDVSLHHHEKIDGSGYPFNLKADSISIMAKMGAVCDVYDAIT 286 Query: 303 SDRPYKKGWDPAESLTAMASWSGHFDKTLLSAFVKTLGIYPIGSLLKMESGRLGLVIRQS 362 S+RPYK GWDPA S+ MA+ +GHFD + AFVK +GIYP GS + ++SGRLG+V+ Q Sbjct: 287 SNRPYKAGWDPARSIRHMAASAGHFDPITMQAFVKAIGIYPTGSCVMLQSGRLGVVVDQR 346 Query: 363 AAGLTKPVVKAFRYDSNGDIIDSEIIDLSDPAASDSI 399 L P VK F +N ++SEIIDL+ +SD I Sbjct: 347 PDQLLTPRVKVFYSTANRMPLESEIIDLAH--SSDKI 381 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.135 0.404 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,271,745 Number of extensions: 50716 Number of successful extensions: 154 Number of sequences better than 1.0e-08: 10 Number of HSP's gapped: 145 Number of HSP's successfully gapped: 10 Length of query: 426 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 338 Effective length of database: 1,203,190 Effective search space: 406678220 Effective search space used: 406678220 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)