BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0004.0095.p (409 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313516|ref|YP_003777608.1| major facilitator superfamily p... 169 4e-43 gi|300313734|ref|YP_003777826.1| permease [Herbaspirillum serope... 155 5e-39 gi|300311567|ref|YP_003775659.1| major facilitator superfamily p... 152 6e-38 gi|300310476|ref|YP_003774568.1| major facilitator superfamily t... 144 2e-35 gi|300310490|ref|YP_003774582.1| major facilitator superfamily p... 141 8e-35 >gi|300313516|ref|YP_003777608.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 437 Score = 169 bits (428), Expect = 4e-43, Method: Compositional matrix adjust. Identities = 126/415 (30%), Positives = 204/415 (49%), Gaps = 26/415 (6%) Query: 3 ILPTVFIVYMLSYFDRVNIAMALPHMTKELGLTSTQGGWIGGAFAWGYALTQLLAGYLAM 62 I+P + + Y+++Y DRVN+ A M+++LG + T G G F GY L ++ + L Sbjct: 29 IMPFLMLCYVIAYLDRVNVGFAKLQMSQDLGFSETVFGLGAGLFFIGYFLFEVPSNILMH 88 Query: 63 KFGARRLVAVCLVLFG---AAAMLTGLATTFWQVLSVRILLGLAEGPVYAGTSILLAQWF 119 K GAR +A ++ +G AA M AT F+ +R LLGLAE Y G + L WF Sbjct: 89 KVGARIWIARIMITWGILSAAFMFVQNATQFY---ILRFLLGLAEAGFYPGIILYLTYWF 145 Query: 120 MKAERGRAFGIWNLGAPLGGFLAGPISGAIIAHYD-------WRVMLIAEGLPAWIFCLI 172 R + ++ G P+ G L P+SG I+ + W+ M + E +PA + + Sbjct: 146 PSHRRAKVIAVFMSGIPVAGILGNPLSGWIMDAFHQNGGLEGWQWMFLIEAIPAVLIGVA 205 Query: 173 WFKSIPKSLDAAAWLPAGDRDTIKRTLDEEQAAHQAPETDPWWTIFNEPAVWLMTLGFGL 232 + + +A WL ++ +++ +D + ++ + I + VWLM L + Sbjct: 206 TVLYLDNDVKSAKWLNDEEKASLQADIDGDAKGKESKHG--FGAIVKDARVWLMCLIYFS 263 Query: 233 NTILLYGITLWLPTVMKSYGQLSETAIGFFAGAPFVMSMLGIYYISRRSDRNGQERRWHA 292 + YG+TLW+PT++K+ G IG + PF +++ + I R +DR +ERRWH Sbjct: 264 FVMGQYGLTLWMPTLVKATGVKGNLEIGLLSAIPFGCAIIAMNLIGRSADRM-RERRWHL 322 Query: 293 AIPTMITGLLMAAAAFVPASLF----YVQIALFILIGFTLKMLMPLIFSRLTEILPLRKA 348 IP LM FV A+LF V IA L + PL +S T L A Sbjct: 323 VIPA-----LMGGVGFVGAALFADNTAVSIASLSLAAAGVLTCAPLFWSLPTAFLSGAAA 377 Query: 349 MPAVAFVSGVGNLIGQFLGPLLVGYVRSISPDYKMSLLMLGVAAFLGGVAIAVSK 403 +A ++ VGNL G F+ P L+GY++ ++ + + ML V +G +A +K Sbjct: 378 AVGIAAINSVGNLAG-FVSPYLIGYLKDLTHNNATGMYMLAVMLVVGSIATLATK 431 >gi|300313734|ref|YP_003777826.1| permease [Herbaspirillum seropedicae SmR1] Length = 431 Score = 155 bits (393), Expect = 5e-39, Method: Compositional matrix adjust. Identities = 117/412 (28%), Positives = 201/412 (48%), Gaps = 15/412 (3%) Query: 3 ILPTVFIVYMLSYFDRVNIAMALPHMTKELGLTSTQGGWIGGAFAWGYALTQLLAGYLAM 62 +LP + + Y+ ++ DR NI MA M +LGL+ G+ F Y + ++ + Sbjct: 22 LLPFLMLCYLFAFIDRGNIGMAALQMNHDLGLSPKMFGFASSLFFIAYFIFEVPSNLALQ 81 Query: 63 KFGARRLVAVCLVLFGAAAMLTGLATTFWQVLSVRILLGLAEGPVYAGTSILLAQWFMKA 122 KFGAR+ +A ++ +G + T L + VR +LG AE + G + L W A Sbjct: 82 KFGARKWLARIMITWGLISACTALVQGPNSLYFVRFILGAAEAGFFPGVVLYLTYWVPSA 141 Query: 123 ERGRAFGIWNLGAPLGGFLAGPISGAIIAHYD-------WRVMLIAEGLPAWIFCLIWFK 175 R R ++ + P F+A P+S A++ D W + + EGLP + L+ Sbjct: 142 YRARIVALFMVAIPAASFVASPVS-ALLLQMDGIGGLRGWHWLFMLEGLPTVVLGLVCLS 200 Query: 176 SIPKSLDAAAWLPAGDRDTIKRTLDEEQAAHQAPETDPWWTIFNEPAVWLMTLGFGLNTI 235 + D A WL R + T+++E A+ A + P W +F++P VW M L + Sbjct: 201 FLTDRPDQARWLSDEQRQWLTETMNKEAASRAAKQHIPLWKLFSQPHVWGMALVCACASA 260 Query: 236 LLYGITLWLPTVMKSYGQLSETAIGFFAGAPFVMSMLGIYYISRRSDRNGQERRWHAAIP 295 + +W P ++KS+G LS G P++++ + + + R SDR G ERRWH AIP Sbjct: 261 AGSVLNVWQPQLLKSFG-LSVMQTGLVNSIPYLIASVLMVWWGRHSDRTG-ERRWHTAIP 318 Query: 296 TMITGLLMAAAAFVPASLFYVQIALFILIG-FTLKMLMPLIFSRLTEILPLRKAMPAVAF 354 M+ G +A A+ + V + +LIG ++ K ++ + L A +A Sbjct: 319 LMLIGGGLALASLANSLAPVVLLLTMVLIGAYSFK---GPFWAMASSWLATGSAAAGLAG 375 Query: 355 VSGVGNLIGQFLGPLLVGYVRSISPDYKMSLL-MLGVAAFLGGVAIAVSKTA 405 ++ V NLIG L + G+V+ + Y M+LL M+G++ G+ + +S+ + Sbjct: 376 INAVSNLIGGGLMVNVYGWVKDATGSYAMALLPMVGLSVVSVGILLFLSRKS 427 >gi|300311567|ref|YP_003775659.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 435 Score = 152 bits (383), Expect = 6e-38, Method: Compositional matrix adjust. Identities = 111/404 (27%), Positives = 190/404 (47%), Gaps = 17/404 (4%) Query: 3 ILPTVFIVYMLSYFDRVNIAMALPHMTKELGLTSTQGGWIGGAFAWGYALTQLLAGYLAM 62 +LP +F+ Y+ SY DRVN+ A M ++L + T G G F GY + ++ + + Sbjct: 28 LLPLLFLCYVASYLDRVNVGFAKLQMLQDLKFSDTVYGLGAGIFFLGYFVFEVPSNMILH 87 Query: 63 KFGARRLVAVCLVLFGAAAMLTGLATTFWQVLSVRILLGLAEGPVYAGTSILLAQWFMKA 122 K GAR +A ++ +G + TT +R LLG+AE + G + L W+ A Sbjct: 88 KVGARLWIARIMITWGIISGAMAYVTTPEMFYIMRFLLGVAEAGFFPGVILYLTYWYPAA 147 Query: 123 ERGRAFGIWNLGAPLGGFLAGPISGAII-------AHYDWRVMLIAEGLPAWIFCLIWFK 175 RG+ ++ G L G + GP+SG I+ H W+ M I E +P+ I ++ Sbjct: 148 RRGKITALFMTGIALSGVIGGPLSGWIMHAMPGVWGHTGWQWMFILEAIPSLILGVMVIL 207 Query: 176 SIPKSLDAAAWLPAGDRDTIKRTLDEEQAAHQAPETDPWWTIFNEPAVWLMTLGFGLNTI 235 + + A WL ++ ++ + E++ + +F VWL L + + Sbjct: 208 YMKDRIRDADWLSEEEKRLLEANISHEESQKEHLSLG---AMFANGRVWLGALIYFCFVM 264 Query: 236 LLYGITLWLPTVMKSYGQLSETAIGFFAGAPFVMSMLGIYYISRRSDRNGQERRWHAAIP 295 LYG++ WLPT++K+ G +G P+ + + I R +D +ERRWH AIP Sbjct: 265 GLYGVSFWLPTIIKATGVSDPLNVGLLTAIPYAFAAAAMVLIGRSADAR-RERRWHVAIP 323 Query: 296 TMI--TGLLMAAAAFVPASLFYVQIALFILIGFTLKMLMPLIFSRLTEILPLRKAMPAVA 353 I GLL++ +L +A L + +PL +S T L A +A Sbjct: 324 AFIGCIGLLLSTQYGHNTTL---AMASLTLASVGILTTLPLFWSLPTAFLSGTAAAAGIA 380 Query: 354 FVSGVGNLIGQFLGPLLVGYVRSISPDYKMSLLMLGVAAFLGGV 397 ++ +GNL G F+ P LVG+++ + + +L + +GG+ Sbjct: 381 LINSLGNLAG-FVSPFLVGWLKDATQSTNAGMYVLAASLVIGGL 423 >gi|300310476|ref|YP_003774568.1| major facilitator superfamily transporter protein [Herbaspirillum seropedicae SmR1] Length = 449 Score = 144 bits (362), Expect = 2e-35, Method: Compositional matrix adjust. Identities = 111/413 (26%), Positives = 197/413 (47%), Gaps = 20/413 (4%) Query: 3 ILPTVFIVYMLSYFDRVNIAMALPHMTKELGLTSTQGGWIGGAFAWGYALTQLLAGYLAM 62 +LP I+++++Y DRVNI H+ ++G+ + G G F GYAL ++ + L Sbjct: 37 VLPLFVIMFIVNYIDRVNIGFVRSHLATDVGIGTAAYGLGAGLFFVGYALFEVPSNLLLQ 96 Query: 63 KFGARRLVAVCLVLFGAAAMLTGLATTFWQVLSVRILLGLAEGPVYAGTSILLAQWFMKA 122 +FGA+ + + +G AA + +R LLG AE + G QW + Sbjct: 97 RFGAKAWLTRIMATWGLAATAMAFVNSETTFYVLRFLLGAAEAGFFPGVIYYFTQWLPAS 156 Query: 123 ERGRAFGIWNLGAPLGGFLAGPISGAII-----AHYDWRVMLIAEGLPAWIFC-LIWF-- 174 ERGRA I+ G+ L L+GPISG ++ W+ M I EG+ + + C +WF Sbjct: 157 ERGRAMAIFLSGSALASILSGPISGGLLQIDGGGLQGWQWMFIIEGMASVLLCGFVWFWL 216 Query: 175 KSIPKSLDAAAWLPAGDRDTIKRTLDEEQA---AHQAPETDPWWTIFNEPAVWLMTLGFG 231 S+P A WL A +R I R + +EQA HQ + P + +P + + + Sbjct: 217 DSMPAD---AKWLTAQERSAITRAIVDEQAERMKHQPAQVSP-RQLLRDPQILIFCFIYF 272 Query: 232 LNTILLYGITLWLPTVMKSYGQLSETAIGFFAGAPFVMSMLGIYYISRRSDRNGQERRWH 291 ++ +YG T WLP++++ G S+ +G F P+++S++ +Y + + R ++ W Sbjct: 273 SISLTIYGATFWLPSIIRKMGSFSDFQVGLFNSIPWLISIVAMYAFAALAARFKHQQAWA 332 Query: 292 AAIPTMITGLLMAAAAFVPASLFYVQIALFILIGFTLKMLMPLIFSRLTEILPLRKAMPA 351 A + M A+ F + +V I F +GF K L + L R A Sbjct: 333 ATALVIAA-AGMFASTFGNSVYAFVSIC-FAAVGF--KAASSLFWPLPQAYLDARIAAGV 388 Query: 352 VAFVSGVGNLIGQFLGPLLVGYVRSISPDYKMSLLMLGVAAFLGGVAIAVSKT 404 +A ++ +GNL G F+ P G++ + + L+ L + + + + ++++ Sbjct: 389 IALINSIGNL-GGFVAPTAFGFLEQRTGSIQGGLMGLAITSLVAAGVVFLARS 440 >gi|300310490|ref|YP_003774582.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 447 Score = 141 bits (356), Expect = 8e-35, Method: Compositional matrix adjust. Identities = 108/409 (26%), Positives = 193/409 (47%), Gaps = 12/409 (2%) Query: 3 ILPTVFIVYMLSYFDRVNIAMALPHMTKELGLTSTQGGWIGGAFAWGYALTQLLAGYLAM 62 ILP +++++Y DRVNI H++ +LG+ + G G F GYAL ++ + L Sbjct: 25 ILPLFVAMFIINYIDRVNIGFVRSHLSTDLGIGAAAFGLGAGLFFVGYALFEVPSNMLLQ 84 Query: 63 KFGARRLVAVCLVLFGAAAMLTGLATTFWQVLSVRILLGLAEGPVYAGTSILLAQWFMKA 122 ++GAR + + +G A + +R LLG+AE + G QW Sbjct: 85 RYGARAWLTRIMFTWGLVAACMAFVQSETSFYFMRFLLGVAEAGFFPGVVYYFTQWLPNH 144 Query: 123 ERGRAFGIWNLGAPLGGFLAGPISGAII-----AHYDWRVMLIAEGLPAWIFCLIWFKSI 177 ERGRA ++ G+ + L+GPI+GA++ + W+ M I EG + +FC++ + + Sbjct: 145 ERGRAMAVFLSGSAIASILSGPITGALLLIEGGGLHGWQWMFIIEGGASMVFCVVVWLVL 204 Query: 178 PKSLDAAAWLPAGDRDTIKRTL--DEEQAAHQAPETDPWWTIFNEPAVWLMTLGFGLNTI 235 + A WL R+ + + ++EQ A P ++ +P + L + + Sbjct: 205 DSTPREAKWLSQEQREALAAVIAAEQEQRAANRPAHVSALSLLRDPQIVLFCFIYFAIQL 264 Query: 236 LLYGITLWLPTVMKSYGQLSETAIGFFAGAPFVMSMLGIYYISRRSDRNGQERRWHAAIP 295 +YG T WLPT++K G ++ +G F P+++++ +Y + + R + + W A Sbjct: 265 TIYGATFWLPTIIKKMGHFNDFQVGLFNSIPWIVAIAAMYLFAVLAARYKKPQFW-VATT 323 Query: 296 TMITGLLMAAAAFVPASLFYVQIALFILIGFTLKMLMPLIFSRLTEILPLRKAMPAVAFV 355 +I+ MA +A + +V + F IGF K L + L R A +A + Sbjct: 324 LVISAAGMAISAVAGPVIGFVAVC-FAGIGF--KAASSLFWPIPQAYLDARIAAAVLALI 380 Query: 356 SGVGNLIGQFLGPLLVGYVRSISPDYKMSLLMLGVAAFLGGVAIAVSKT 404 + +GNL G F P GY+ + + L L A+ L V + +++T Sbjct: 381 NSLGNL-GGFFAPATFGYLEQKTGSIQGGLFALSGASLLAAVLVFLART 428 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.328 0.141 0.449 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,266,848 Number of extensions: 52495 Number of successful extensions: 413 Number of sequences better than 1.0e-08: 30 Number of HSP's gapped: 354 Number of HSP's successfully gapped: 30 Length of query: 409 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 322 Effective length of database: 1,207,925 Effective search space: 388951850 Effective search space used: 388951850 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 132 (55.5 bits)