BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0004.0081.p (359 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312336|ref|YP_003776428.1| methyl-accepting chemotaxis tra... 324 7e-90 gi|300310668|ref|YP_003774760.1| methyl-accepting chemotaxis tra... 269 3e-73 gi|300313938|ref|YP_003778030.1| methyl-accepting chemotaxis tra... 254 1e-68 gi|300310594|ref|YP_003774686.1| methyl-accepting chemotaxis pro... 245 3e-66 gi|300312730|ref|YP_003776822.1| methyl-accepting chemotaxis pro... 214 6e-57 >gi|300312336|ref|YP_003776428.1| methyl-accepting chemotaxis transducer transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 536 Score = 324 bits (830), Expect = 7e-90, Method: Compositional matrix adjust. Identities = 183/357 (51%), Positives = 251/357 (70%) Query: 1 MKRLSNMKIGDRLAIGFAIILALSIVSTGIGIWRMQVVSKVTRAMVDESVRKERLISSLN 60 M + NM IG RL +GFA+IL SI+ GIG+WR+ V++ TR M+ E ++ ERL++ Sbjct: 1 MNIIGNMNIGRRLTLGFALILTFSIIIAGIGLWRLDHVAQATREMMAEPLKTERLMADWY 60 Query: 61 TNLRGGILRTIAILRSTDQMLNAFFLNEDLIAKKDAIALQKQIGPLLVSDEEKQLYKQVL 120 TNL GI RTIAI +S+D L +F E + K + QKQ+ LL + EEK L++++ Sbjct: 61 TNLVAGIRRTIAIAKSSDPALGPYFAAEVAASSKSSGEYQKQVEALLSTPEEKALFQKIG 120 Query: 121 EQQVNYAKINFHLMELKSNGDAEEGNQILVQEFVPASKRYQATLQKLLEWEQAEIDKTSL 180 E + Y + + K+ G+ EE +IL Q FVP + YQ T++KL+++++ E+D T+ Sbjct: 121 ELRKLYLSSRDEINKAKAAGNTEEAMRILDQVFVPNATAYQDTMRKLVDFQRKEMDDTAR 180 Query: 181 QIDVIAAQSRILLLIVEVAALLFGVSCAWLLTRSIVWPLRQAVEISRKVADGDLTTEIQI 240 +ID IAA+SR L+L++E LL G+ A LT I PL +AV++SR VA+GDL++EI + Sbjct: 181 EIDAIAAKSRTLILVLEGLILLLGIVFARYLTLGITKPLHRAVDVSRCVAEGDLSSEIAV 240 Query: 241 KRTDEVGQLLMSLQGMNTNLRNIVNNVRTGTVTIATASREIAAGNMDLSSRTESQASALE 300 DE GQLL SL+ MN +LR IV+NVR+GT TI+TAS EIAAGN+DLSSRTE QAS+L Sbjct: 241 TSRDETGQLLQSLKDMNQSLRGIVSNVRSGTDTISTASSEIAAGNLDLSSRTEQQASSLA 300 Query: 301 ETASAMEELISTVRSNADNAREASELASSASSIAIEGGNAVDNMVQTMGEINASSHQ 357 ETASAMEELISTVR NADNAR+AS+LA+SAS++A +GG V +V TMG INASS + Sbjct: 301 ETASAMEELISTVRQNADNARQASQLANSASAVAEQGGGVVSQVVDTMGAINASSRK 357 >gi|300310668|ref|YP_003774760.1| methyl-accepting chemotaxis transducer transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 515 Score = 269 bits (687), Expect = 3e-73, Method: Compositional matrix adjust. Identities = 147/354 (41%), Positives = 229/354 (64%) Query: 4 LSNMKIGDRLAIGFAIILALSIVSTGIGIWRMQVVSKVTRAMVDESVRKERLISSLNTNL 63 + +++IG RL GFA++L S+V +GIWR++ V+ T+ M+D ++ ERL+S NT L Sbjct: 2 IKDIRIGSRLGAGFAVVLVFSMVVAAVGIWRLKTVAVQTQQMMDVPLKTERLVSQWNTLL 61 Query: 64 RGGILRTIAILRSTDQMLNAFFLNEDLIAKKDAIALQKQIGPLLVSDEEKQLYKQVLEQQ 123 I RT +++S D L AF E + K++ K + LL S+EEK+++ + + Sbjct: 62 LIAIQRTTTVVKSHDPELEAFLAKEAAASSKESAQTLKDVEQLLASEEEKKMFAAINVAR 121 Query: 124 VNYAKINFHLMELKSNGDAEEGNQILVQEFVPASKRYQATLQKLLEWEQAEIDKTSLQID 183 + I + K GDA + ++ +++V +K Q ++ LL++E+A ID+ S QI Sbjct: 122 KQFLTIRDQIYAAKKQGDAAQVEELYQKQYVGIAKSTQDAMRALLDFERARIDQISAQIQ 181 Query: 184 VIAAQSRILLLIVEVAALLFGVSCAWLLTRSIVWPLRQAVEISRKVADGDLTTEIQIKRT 243 A+ S+ ++++EV LL G++ A LLTRSI P+ A+ IS++VA GDLT+ Q Sbjct: 182 QDASSSQHQIMLLEVVILLCGIAFAVLLTRSITRPVHVALAISQRVAAGDLTSRAQAMGR 241 Query: 244 DEVGQLLMSLQGMNTNLRNIVNNVRTGTVTIATASREIAAGNMDLSSRTESQASALEETA 303 DE+GQL+ SLQGM+ L +V+ VR G +IA AS EIA GN+DLS+RTE QA ALE+TA Sbjct: 242 DELGQLVASLQGMSERLHGVVSTVRHGADSIAMASGEIANGNLDLSARTEEQAGALEQTA 301 Query: 304 SAMEELISTVRSNADNAREASELASSASSIAIEGGNAVDNMVQTMGEINASSHQ 357 +++EEL S+VR+N+DNA +A+EL S+A+S+A +GG +D ++ TM IN SS + Sbjct: 302 ASIEELTSSVRTNSDNAHQANELVSTAASVAGQGGAVMDQLISTMATINDSSRK 355 >gi|300313938|ref|YP_003778030.1| methyl-accepting chemotaxis transducer transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 533 Score = 254 bits (648), Expect = 1e-68, Method: Compositional matrix adjust. Identities = 147/355 (41%), Positives = 217/355 (61%), Gaps = 2/355 (0%) Query: 4 LSNMKIGDRLAIGFAIILALSIVSTGIGIWRMQVVSKVTRAMVDESVRKERLISSLNTNL 63 + N+KIG RLAIGF +L L V +G+WR+ + T M +++KER + + Sbjct: 1 MKNLKIGVRLAIGFIALLMLMAVMVALGVWRLGSIGDATDDMTRIALKKERDAVQWHAAI 60 Query: 64 RGGILRTIAILRSTDQMLNAFFLNEDLIAKKDAIA-LQKQIGPLLVSDEEKQLYKQVLEQ 122 + +RT A+++S D A+F + + A+ I+ LQK++ + EEK+L+ +V + Sbjct: 61 KENGVRTFAVMKSDDDAFQAYF-QKQIDAQSARISQLQKEVEAAITDAEEKRLFDEVGKL 119 Query: 123 QVNYAKINFHLMELKSNGDAEEGNQILVQEFVPASKRYQATLQKLLEWEQAEIDKTSLQI 182 + Y + ++K+ GD ++ V Y + + ++++ ID+T+ I Sbjct: 120 RAAYRATRQSIFDVKTAGDDARAREMTDTTLVKMMDDYADGVLRYADFQKQVIDQTAAAI 179 Query: 183 DVIAAQSRILLLIVEVAALLFGVSCAWLLTRSIVWPLRQAVEISRKVADGDLTTEIQIKR 242 + R +LL + ++ G + AWLLTRSI PL QAV I+ VA GDLT+ I Sbjct: 180 NRDYRSGRSMLLALGAIEIVLGAALAWLLTRSITRPLNQAVTIAETVAAGDLTSRIDSSS 239 Query: 243 TDEVGQLLMSLQGMNTNLRNIVNNVRTGTVTIATASREIAAGNMDLSSRTESQASALEET 302 DE G+LL +L+ MN NL +IV VR GT TI TASREIA GN+DLS+RTE+QA +LEET Sbjct: 240 RDETGKLLAALKTMNGNLLDIVGRVRMGTETINTASREIATGNLDLSARTEAQAGSLEET 299 Query: 303 ASAMEELISTVRSNADNAREASELASSASSIAIEGGNAVDNMVQTMGEINASSHQ 357 ASAME+L STV+ NADNAR+A+ELA+SAS +A +GG VD +V TMG I+ASS + Sbjct: 300 ASAMEQLTSTVKQNADNARQANELAASASQVATQGGAVVDQVVATMGAIHASSQK 354 >gi|300310594|ref|YP_003774686.1| methyl-accepting chemotaxis protein I [Herbaspirillum seropedicae SmR1] Length = 541 Score = 245 bits (626), Expect = 3e-66, Method: Compositional matrix adjust. Identities = 163/364 (44%), Positives = 224/364 (61%), Gaps = 11/364 (3%) Query: 1 MKRLSNMKIGDRLAIGFAIILALSIVSTGIGIWRMQVVSKVTRAMVDESVRKERLISSLN 60 M+ +NMKIG RLA+GFA+ILA + V + IWR+Q ++ T M+ E + KERL+S Sbjct: 1 MRYFANMKIGRRLALGFAVILAAASVVVALSIWRLQGIAAATDEMMHEPLTKERLVSDWY 60 Query: 61 TNLRGGILRTIAILRSTDQMLNAFFLNEDLIAKKDAIALQKQIGPLLVSDEEKQLYKQVL 120 + + RT AI +S+D L AFF ++ A + + QK I L+ SD+EK ++ ++ Sbjct: 61 RTIHTSVRRTTAIAKSSDPSLAAFFADDAAAASRMSTEQQKAIEALIESDQEKAVFTKLG 120 Query: 121 EQQVNYAKINFHLMELKSNGDAEEGNQILVQEFVPASKRYQATLQKLLEWEQAEIDKTSL 180 E + +Y K + + K+ G EE QIL F A K Y LQ+LL ++A ID+ + Sbjct: 121 EVRKDYIKYRDAISKAKAEGRTEEAAQILAGPFDVAGKGYLDLLQQLLTLQRANIDQVAA 180 Query: 181 QIDVIAAQSRILLLIVEVAALLFGVSCAWLLTRS----IVWPLRQAVEISRKVADGDLTT 236 I + +SR L+L + ++ G WL R+ I PL QAVE++ VA GDLT Sbjct: 181 HIQTVYRESRNLMLALAFLQVVLG----WLFARTLALGITRPLEQAVEVAEAVAAGDLTH 236 Query: 237 EIQIK---RTDEVGQLLMSLQGMNTNLRNIVNNVRTGTVTIATASREIAAGNMDLSSRTE 293 I + R DE G+L+ +L M NL IV VR GT IATAS+EIA GN+DLS+RTE Sbjct: 237 RIDERLAARGDETGKLMHALHAMTGNLARIVGQVRGGTDAIATASKEIATGNLDLSARTE 296 Query: 294 SQASALEETASAMEELISTVRSNADNAREASELASSASSIAIEGGNAVDNMVQTMGEINA 353 QA ALEETASAMEEL STV NADNAR+A++LA+SASS+A +GG V ++ TMG IN Sbjct: 297 RQAGALEETASAMEELTSTVSQNADNARQANQLAASASSVAQQGGEVVSQVISTMGSINE 356 Query: 354 SSHQ 357 SS + Sbjct: 357 SSRK 360 >gi|300312730|ref|YP_003776822.1| methyl-accepting chemotaxis protein [Herbaspirillum seropedicae SmR1] Length = 583 Score = 214 bits (546), Expect = 6e-57, Method: Compositional matrix adjust. Identities = 137/366 (37%), Positives = 218/366 (59%), Gaps = 22/366 (6%) Query: 3 RLSNMKIGDRLAIGFAIILALSIVSTGIGIWRMQVVSKVTRAMVDESVRKERLISSLN-- 60 R +N+ I RLAI F+I++ + +G+ R+ ++K +V++ +K +I++++ Sbjct: 2 RFNNLSIATRLAIAFSILILVIAGIVALGLARLADINKALDLVVNDRYKKIAIINTISGK 61 Query: 61 -----TNLRGGILRT----IAILRSTDQMLNAFFLNEDLIAKKDAIALQKQIGPLLVSDE 111 +LR +L T IA + T + L+A + L ++ L+ + E Sbjct: 62 VDDVAISLRNQLLMTDPAQIAAEQKTIESLSA-----------ENTKLYDELTRLVANPE 110 Query: 112 EKQLYKQVLEQQVNYAKINFHLMELKSNGDAEEGNQILVQEFVPASKRYQATLQKLLEWE 171 K +V E + +YA + + +L+S+GD + ++++ E VP K Y + L +L + + Sbjct: 111 AKAKLDKVTETRKSYAALRKQIDKLQSSGDKDAAIKLMLSEMVPLQKIYFSQLDELSDTQ 170 Query: 172 QAEIDKTSLQIDVIAAQSRILLLIVEVAALLFGVSCAWLLTRSIVWPLRQAVEISRKVAD 231 Q +D++ Q + +R++++I V A F AW ++RSI PL +AVE++ VA Sbjct: 171 QKLMDQSVDQAEAAYETTRMIMVIAGVLAATFAAFIAWTISRSITRPLSRAVEVAETVAA 230 Query: 232 GDLTTEIQIKRTDEVGQLLMSLQGMNTNLRNIVNNVRTGTVTIATASREIAAGNMDLSSR 291 GDLT I DE G+LL +L MN L+ IV VR GT ++ TAS +IA GN+DLSSR Sbjct: 231 GDLTAVIAAHSNDETGRLLQALAAMNQKLKAIVLEVRRGTDSMVTASTQIATGNLDLSSR 290 Query: 292 TESQASALEETASAMEELISTVRSNADNAREASELASSASSIAIEGGNAVDNMVQTMGEI 351 TE QASALEETAS++EEL S+V+ NAD+ R++SELA++AS +A +GG AV +VQTMG I Sbjct: 291 TEEQASALEETASSLEELTSSVKQNADHTRKSSELAANASQVANQGGLAVKQVVQTMGAI 350 Query: 352 NASSHQ 357 N SSH+ Sbjct: 351 NDSSHK 356 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.315 0.129 0.338 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 803,772 Number of extensions: 25721 Number of successful extensions: 348 Number of sequences better than 1.0e-08: 65 Number of HSP's gapped: 277 Number of HSP's successfully gapped: 65 Length of query: 359 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 273 Effective length of database: 1,212,660 Effective search space: 331056180 Effective search space used: 331056180 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 131 (55.1 bits)