BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0004.0075.p (411 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310161|ref|YP_003774253.1| aminohydrolase [Herbaspirillum ... 390 e-110 gi|300312475|ref|YP_003776567.1| metal-dependent amidase/aminoac... 376 e-105 gi|300310472|ref|YP_003774564.1| hippurate hydrolase [Herbaspiri... 356 2e-99 >gi|300310161|ref|YP_003774253.1| aminohydrolase [Herbaspirillum seropedicae SmR1] Length = 402 Score = 390 bits (1003), Expect = e-110, Method: Compositional matrix adjust. Identities = 199/396 (50%), Positives = 254/396 (64%), Gaps = 6/396 (1%) Query: 15 IKEIETSHAEFSHIRRDLHAHPELAFQEVRTADAIVASLQNWGIPVHRGLGGTGLVGIIR 74 IKE+ + IRR LHAHPEL F+E RTA + L+++G+ V +GG G+V +R Sbjct: 8 IKEL-PKEMDLVSIRRHLHAHPELRFEEKRTASFVADMLRSYGLTVAENIGGYGVVATLR 66 Query: 75 NGRSTRAIGLRADMDALPIQELNTFEHASQYPGKMHACGHDGHTAMLLAAAWHLNATRNF 134 G+STRAI LRADMDALP+ E N F H S GKMHACGHDGHT MLL AA L+ F Sbjct: 67 KGKSTRAIALRADMDALPMSEQNDFSHISTCAGKMHACGHDGHTTMLLGAARRLSREVEF 126 Query: 135 DGTVYLIFQPAEEGKGGAASMIKDGLFEQFEMESVYAIHNWPGMPLGSFGVNSGAIMASS 194 DGTV+ +FQPAEEG GA MI+DGLFE+F ++++ +HNWPG+P GSFG+ G +MASS Sbjct: 127 DGTVHFVFQPAEEGGAGARLMIEDGLFERFPADAIFGVHNWPGLPAGSFGLRPGPLMASS 186 Query: 195 NEFKITLRGKGSHAALPHHSIDPVAAMAALSQALQTILTRNKRPNDAAVLSITQMQAGEA 254 N F TL G+G+H A PH SIDPV A A L+ A Q+I+TRN PN AV+S+TQ+ G A Sbjct: 187 NTFSATLFGRGAHGAQPHRSIDPVLAAAQLTLAWQSIVTRNINPNHRAVISVTQLHTGTA 246 Query: 255 SNVIPDFGWIGGTVRTFSIDVLDLIESRMREMSEHLAHGFGCDAEVIFNRNYPPTINHRA 314 NVIP+ + GTVR+F + LDLIE RM ++E + F + F R YP +NH Sbjct: 247 DNVIPEQATLSGTVRSFDAETLDLIEHRMAAIAEAIGQMFDLRVQFHFERLYPAVVNHPE 306 Query: 315 ETAICVEVMRDVVGAENVDADIEAPLSSEDFSFMLLAKPGCYALIGNG---DGGHRDHGH 371 T ++ M VGAE VD D E +SEDF+F L KPGCYA +GNG DG + G Sbjct: 307 ATQQAMQAMMAAVGAEQVDGDTEPAFASEDFAFYLQHKPGCYAFLGNGVIADG--KQAGS 364 Query: 372 GLGPCMLHNTSYDFNDNLLPIGATYWVRLTERLLAL 407 LH+ YDFND+++ G YWV L + L + Sbjct: 365 QATSRELHSPFYDFNDDIIDAGVAYWVSLVRQYLGV 400 >gi|300312475|ref|YP_003776567.1| metal-dependent amidase/aminoacylase/carboxypeptidase [Herbaspirillum seropedicae SmR1] Length = 399 Score = 376 bits (966), Expect = e-105, Method: Compositional matrix adjust. Identities = 193/389 (49%), Positives = 247/389 (63%), Gaps = 12/389 (3%) Query: 24 EFSHIRRDLHAHPELAFQEVRTADAIVASLQNWGIPVHRGLGGTGLVGIIRNGRSTRAIG 83 E + IRR LH HPEL+F+EV TA + L+ WG V R +GG GLV +R G S R+IG Sbjct: 21 EITAIRRHLHQHPELSFEEVDTAALVAQRLEQWGYAVTRHIGGNGLVATLRVGSSARSIG 80 Query: 84 LRADMDALPIQELNTFEHASQYPGKMHACGHDGHTAMLLAAAWHLNATRNFDGTVYLIFQ 143 LRADMDALPIQE + AS PG MHACGHDGHTAMLL AA HL TR FDGT+ LIFQ Sbjct: 81 LRADMDALPIQEETGLDWASVKPGAMHACGHDGHTAMLLGAARHLARTRRFDGTLNLIFQ 140 Query: 144 PAEEG--KGGAASMIKDGLFEQFEMESVYAIHNWPGMPLGSFGVNSGAIMASSNEFKITL 201 PAEE GA M+ DGLFE+F E+V+ IHN PG+ G+F SG MA+ + KI + Sbjct: 141 PAEEAGFDSGAQKMLADGLFERFPCEAVFGIHNHPGVEAGTFMFRSGPFMAACDTVKIRI 200 Query: 202 RGKGSHAALPHHSIDPVAAMAALSQALQTILTRNKRPNDAAVLSITQMQAGEASNVIPDF 261 G+GSHAA PH S+DPV A+L ALQT+++RN P D+AV+++ + AG+ASNVIP+F Sbjct: 201 TGRGSHAARPHLSVDPVVVAASLVMALQTVVSRNIDPMDSAVVTVGSLHAGKASNVIPEF 260 Query: 262 GWIGGTVRTFSIDVLDLIESRMREMSEHLAHGFGCDAEVIFNRNYPPTINHRAETAICVE 321 + +VR+F +V +L+E R+R + A +G AE+ + R YP +N AET Sbjct: 261 ATMELSVRSFKPEVRELLEQRIRALVSTHAQSYGAQAEIDYLRGYPVLVNSDAETDFARS 320 Query: 322 VMRDVVGAENVDADIEAPLSSEDFSFMLLAKPGCYALIGNGDGGHRDHGHGLGPCMLHNT 381 V ++VG E V A SEDF++ L +PGC+ +GNG G MLHN Sbjct: 321 VAEELVGPEKVIAPFGPIAGSEDFAYFLRQRPGCFLRVGNGQ----------GKPMLHNA 370 Query: 382 SYDFNDNLLPIGATYWVRLTERLLALTNQ 410 YDFND +PIGA YW RL ER + L + Sbjct: 371 GYDFNDANIPIGAAYWTRLVERWMPLQGE 399 >gi|300310472|ref|YP_003774564.1| hippurate hydrolase [Herbaspirillum seropedicae SmR1] Length = 403 Score = 356 bits (914), Expect = 2e-99, Method: Compositional matrix adjust. Identities = 182/380 (47%), Positives = 230/380 (60%), Gaps = 7/380 (1%) Query: 23 AEFSHIRRDLHAHPELAFQEVRTADAIVASLQNWGIPVHRGLGGTGLVGIIRNGRSTRAI 82 A ++ +RRDLHAHPEL F+E RTAD +V LQ+ G V RGLGGTG+V + R I Sbjct: 16 AHWAGLRRDLHAHPELRFEEHRTADVVVRELQSLGYTVTRGLGGTGVVASLAGADPQRGI 75 Query: 83 GLRADMDALPIQELNTFEHASQYPGKMHACGHDGHTAMLLAAAWHLNATRNFDGTVYLIF 142 LRAD+DALPI E N F HAS G MHACGHDGHT MLL AA L G+V+ +F Sbjct: 76 VLRADLDALPIVEANDFAHASCTHGVMHACGHDGHTVMLLGAARLLKQGPMLPGSVHFVF 135 Query: 143 QPAEEGKGGAASMIKDGLFEQFEMESVYAIHNWPGMPLGSFGVNSGAIMASSNEFKITLR 202 QP EEG GA MI DGLFEQF E+V+ +HNWPG+P G FG+ G IMA+ + KIT+ Sbjct: 136 QPGEEGGAGARKMIDDGLFEQFPTEAVFGMHNWPGLPAGQFGLRPGPIMAAGSRLKITIT 195 Query: 203 GKGSHAALPHHSIDPVAAMAALSQALQTILTRNKRPNDAAVLSITQMQAGEASNVIPDFG 262 GKG+HAA PH +DP+ ++ QTI R+K P D AV+S+ AG+ NVIPD Sbjct: 196 GKGAHAAQPHLGLDPIPLACSMVLQCQTIAARHKDPVDPAVISVCMFHAGDTDNVIPDRA 255 Query: 263 WIGGTVRTFSIDVLDLIESRMREMSEHLAHGFGCDAEVIFNRNYPPTINHRAETAICVEV 322 + GT+RT S + ++ +R M LA +G EV F + YP TIN AET C V Sbjct: 256 ELRGTIRTLSSTLQQKLQEDIRRMCHALAEAYGAKVEVEFFQYYPATINTPAETDFCERV 315 Query: 323 MRDVVGAENVDADIEAPLSSEDFSFMLLAKPGCYALIGNGDGGHRDHGHGLGPCMLHNTS 382 +R G + + I A ++SEDF FML +PG Y LIGN H LH+ Sbjct: 316 IRQTFGDDRIRTGIPANMTSEDFGFMLEERPGAYVLIGNAPQDRASHS-------LHHPH 368 Query: 383 YDFNDNLLPIGATYWVRLTE 402 YDFND+++ G YWV L + Sbjct: 369 YDFNDDIIEAGVRYWVALAQ 388 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.135 0.409 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,267,479 Number of extensions: 53069 Number of successful extensions: 145 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 141 Number of HSP's successfully gapped: 3 Length of query: 411 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 323 Effective length of database: 1,203,190 Effective search space: 388630370 Effective search space used: 388630370 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 132 (55.5 bits)