BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0004.0071.p (154 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313708|ref|YP_003777800.1| AsnC family transcription regul... 232 1e-62 gi|300312760|ref|YP_003776852.1| transcription regulator protein... 133 6e-33 gi|300313266|ref|YP_003777358.1| AsnC family transcription regul... 94 5e-21 gi|300311006|ref|YP_003775098.1| transcription regulator protein... 91 5e-20 gi|300312130|ref|YP_003776222.1| glutamate uptake transcription ... 90 8e-20 >gi|300313708|ref|YP_003777800.1| AsnC family transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 159 Score = 232 bits (591), Expect = 1e-62, Method: Compositional matrix adjust. Identities = 113/154 (73%), Positives = 128/154 (83%) Query: 1 MKINLDELDRRILNALQEDAAQTNFQLAEHVHASPPTCLRRVKRLVEAGVITRQVAILAP 60 M LD+LDRRIL ALQ DA+QTN +LA VHASPPTCLRRVKRLVE GVI RQVA+L Sbjct: 1 MTFELDDLDRRILKALQADASQTNQELAARVHASPPTCLRRVKRLVEGGVIARQVALLDA 60 Query: 61 EKLGPALTAIVEITLDQQGDERQVEFEGIVAAEPAVLQCYRVSPGPDFVLMVQVADMPAY 120 +K+ ALTA+VEITLD+QG+E Q FE +VA EPAV QCYRVSPGPDFVL+V V DM +Y Sbjct: 61 QKVAAALTAVVEITLDRQGEEEQQAFEALVADEPAVQQCYRVSPGPDFVLVVLVRDMSSY 120 Query: 121 HALAHRLFATHQNVRNVRSYFSIHRSKFETRIAV 154 HALAHRLFA+ +NVRNVRSYFSIHRSKFET I + Sbjct: 121 HALAHRLFASQRNVRNVRSYFSIHRSKFETAIPI 154 >gi|300312760|ref|YP_003776852.1| transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 170 Score = 133 bits (335), Expect = 6e-33, Method: Compositional matrix adjust. Identities = 67/150 (44%), Positives = 97/150 (64%) Query: 4 NLDELDRRILNALQEDAAQTNFQLAEHVHASPPTCLRRVKRLVEAGVITRQVAILAPEKL 63 LD++DR++LN LQ+D + LAE +H S PTCLRR++ L EAG+I+ +VA++ P L Sbjct: 7 TLDKVDRKLLNMLQKDNQISTRVLAEKLHISQPTCLRRIRDLREAGIISAEVAMVDPFAL 66 Query: 64 GPALTAIVEITLDQQGDERQVEFEGIVAAEPAVLQCYRVSPGPDFVLMVQVADMPAYHAL 123 G + A +EI+L+ Q D+ +FE +A E V+QCY VS D+ L V V DM AY+ Sbjct: 67 GYGMLAFLEISLNNQSDQHMQDFEQRMAKEAEVMQCYFVSGEYDYFLAVHVIDMDAYYQF 126 Query: 124 AHRLFATHQNVRNVRSYFSIHRSKFETRIA 153 R+ + NVR+ +S F + R+KF TRIA Sbjct: 127 VRRVISGSGNVRHFQSRFPMKRAKFTTRIA 156 >gi|300313266|ref|YP_003777358.1| AsnC family transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 159 Score = 94.0 bits (232), Expect = 5e-21, Method: Compositional matrix adjust. Identities = 50/148 (33%), Positives = 84/148 (56%) Query: 3 INLDELDRRILNALQEDAAQTNFQLAEHVHASPPTCLRRVKRLVEAGVITRQVAILAPEK 62 + LD D RILN LQE+++ +N +LA ++ SP L RVKRL G+I+R VA+ P Sbjct: 1 MELDTTDLRILNILQENSSISNLELASRINLSPSPTLARVKRLESEGIISRYVALADPHL 60 Query: 63 LGPALTAIVEITLDQQGDERQVEFEGIVAAEPAVLQCYRVSPGPDFVLMVQVADMPAYHA 122 LG + V++ L++QG E +FE V+A V++ Y ++ D++L + V D+ Sbjct: 61 LGLKVNVFVKVILERQGAEALAQFETAVSAFDEVMEVYLMTGDEDYLLRIVVPDLLTLEH 120 Query: 123 LAHRLFATHQNVRNVRSYFSIHRSKFET 150 ++N+RS F++ + K++T Sbjct: 121 FIVDHLTKIPGIKNIRSSFALKQIKYKT 148 >gi|300311006|ref|YP_003775098.1| transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 172 Score = 90.9 bits (224), Expect = 5e-20, Method: Compositional matrix adjust. Identities = 52/145 (35%), Positives = 74/145 (51%) Query: 3 INLDELDRRILNALQEDAAQTNFQLAEHVHASPPTCLRRVKRLVEAGVITRQVAILAPEK 62 IN+D D +L LQ D TN L + VH S RRV+RL EA VI R A+L P Sbjct: 4 INVDRYDLALLTELQRDGHITNSALGQKVHLSTSQISRRVQRLEEAHVIERYTAVLDPAV 63 Query: 63 LGPALTAIVEITLDQQGDERQVEFEGIVAAEPAVLQCYRVSPGPDFVLMVQVADMPAYHA 122 +G +TA +TLD+QG+ R FE V P V +C+ V+ D++L + D+ A+ Sbjct: 64 VGLGVTAFTTVTLDRQGETRGELFEKAVETMPEVTECFSVTGEGDYILRIVAPDLAAFSE 123 Query: 123 LAHRLFATHQNVRNVRSYFSIHRSK 147 V NV+S ++ + K Sbjct: 124 FMMTRLLRLPGVTNVKSNITLKKVK 148 >gi|300312130|ref|YP_003776222.1| glutamate uptake transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 154 Score = 90.1 bits (222), Expect = 8e-20, Method: Compositional matrix adjust. Identities = 53/146 (36%), Positives = 83/146 (56%), Gaps = 1/146 (0%) Query: 5 LDELDRRILNALQEDAAQTNFQLAEHVHASPPTCLRRVKRLVEAGVITRQVAILAPEKLG 64 +D DRRIL LQED+ + ++AE V+ S C RR++RL GVI R+V +L EKL Sbjct: 2 IDRTDRRILEILQEDSTRAVAEIAECVNLSTTPCWRRIQRLEAEGVIKRRVVLLDAEKLN 61 Query: 65 PALTAIVEITLDQQGDERQVEFEGIVAAEPAVLQCYRVSPGPDFVLMVQVADMPAYHALA 124 ++T +VEI Q +F +V P V++ +R+S D++L V V ++ AY ++ Sbjct: 62 VSVTVLVEIKTRQHSASWLKKFRDVVTCIPEVVEVHRMSGHIDYMLKVMVPNIGAYDSVY 121 Query: 125 HRLFATHQNVRNVRSYFSIHRSKFET 150 RL + + + +V S FS+ K T Sbjct: 122 KRLISAVE-LDDVSSSFSMETLKSTT 146 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.323 0.135 0.386 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 460,778 Number of extensions: 17817 Number of successful extensions: 78 Number of sequences better than 1.0e-08: 9 Number of HSP's gapped: 77 Number of HSP's successfully gapped: 9 Length of query: 154 Length of database: 1,619,870 Length adjustment: 77 Effective length of query: 77 Effective length of database: 1,255,275 Effective search space: 96656175 Effective search space used: 96656175 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.5 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.5 bits) S2: 126 (53.1 bits)