BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0004.0053.p (336 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313692|ref|YP_003777784.1| UDP-glucose 4-epimerase [Herbas... 534 e-153 gi|300311327|ref|YP_003775419.1| UDP-glucose 4-epimerase [Herbas... 428 e-121 gi|300313483|ref|YP_003777575.1| NAD_dependent epimerase/dehydra... 107 1e-24 gi|300313479|ref|YP_003777571.1| UDP-galactose-4-epimerase [Herb... 101 9e-23 gi|300312049|ref|YP_003776141.1| UDP-glucose 4-epimerase [Herbas... 92 6e-20 >gi|300313692|ref|YP_003777784.1| UDP-glucose 4-epimerase [Herbaspirillum seropedicae SmR1] Length = 336 Score = 534 bits (1376), Expect = e-153, Method: Compositional matrix adjust. Identities = 250/335 (74%), Positives = 292/335 (87%) Query: 1 MNILVTGGAGYIGSHTCVELLKAGHQVVVFDNFCNSFPESVRRVEKITGKTLALVEGDIR 60 MNILVTGGAGYIGSHTCVELLKAGH+V+VFDNFCNS PESV+RV++ITGKTL+LV+GDIR Sbjct: 1 MNILVTGGAGYIGSHTCVELLKAGHEVIVFDNFCNSSPESVKRVQQITGKTLSLVKGDIR 60 Query: 61 DREALEKALRSYECDAVIHFAGLKAVGESVAKPLEYYDNNVVGTVRLLEAMQATGVKTIV 120 +RE LE LR ++C+AVIHFAGLKAVGESV +PL YYDNNVVGTVRLLEAMQATGVKTIV Sbjct: 61 NREQLESVLREFKCEAVIHFAGLKAVGESVEQPLMYYDNNVVGTVRLLEAMQATGVKTIV 120 Query: 121 FSSSATVYGVPQFLPYTEDHPLSSTSPYGRGKLMVEDILRDYHVAQPDSRIALLRYFNPV 180 FSSSATVYG PQ+LP E HPLS+++PYGR K+++ED+LRDY+ A PD +IALLRYFNPV Sbjct: 121 FSSSATVYGDPQYLPLDEAHPLSASNPYGRSKIVIEDMLRDYYRAHPDWKIALLRYFNPV 180 Query: 181 GAHHSGLIGEDPQGVPNNLMPFVTQVAIGKRDRLQIWGGDYDTHDGTGVRDYIHVEDLAV 240 GAH SGLIGEDP G+PNNLMPFV QVAIG+R+RL IWGGDY T DGTGVRD+IHV DLA Sbjct: 181 GAHESGLIGEDPGGIPNNLMPFVAQVAIGRRERLNIWGGDYPTPDGTGVRDFIHVVDLAR 240 Query: 241 GHVKALEHLRQPQCFAVNLGSGRGHSVLEVVKAFEQACGKSIPYEIGPRRAGDLPAYYAD 300 GHV AL+ L PQCFAVNLG+G G+SVL+V+KA+E+ACGK++PY I RR GD+ + YA+ Sbjct: 241 GHVAALQALDAPQCFAVNLGTGVGYSVLDVLKAYEKACGKTLPYAIAERRPGDIASCYAN 300 Query: 301 VTLAKSLLGWKATRSLETMCADSWRWQQANPDGFR 335 AK LGW+AT LE MCADSW+WQQ NP+GF+ Sbjct: 301 PAEAKRKLGWEATLGLEAMCADSWKWQQMNPNGFK 335 >gi|300311327|ref|YP_003775419.1| UDP-glucose 4-epimerase [Herbaspirillum seropedicae SmR1] Length = 343 Score = 428 bits (1100), Expect = e-121, Method: Compositional matrix adjust. Identities = 208/327 (63%), Positives = 247/327 (75%), Gaps = 1/327 (0%) Query: 2 NILVTGGAGYIGSHTCVELLKAGHQVVVFDNFCNSFPESVRRVEKITGKTLALVEGDIRD 61 ILVTGGAGYIGSHTCVEL+ AG+ VVV DN CNS + R+ +I+G+ V+GDIRD Sbjct: 5 TILVTGGAGYIGSHTCVELIHAGYAVVVLDNLCNSRASVIDRIAQISGQRPHFVQGDIRD 64 Query: 62 REALEKALRSYECDAVIHFAGLKAVGESVAKPLEYYDNNVVGTVRLLEAMQATGVKTIVF 121 R L+ +S+ +AVIHFAGLKAVGESVA+PL YYDNNV G+ L EAM A GVK IVF Sbjct: 65 RAVLDGIFKSHRIEAVIHFAGLKAVGESVAQPLRYYDNNVHGSNVLFEAMAAHGVKNIVF 124 Query: 122 SSSATVYGVPQFLPYTEDHPLSSTSPYGRGKLMVEDILRDYHVAQPDSRIALLRYFNPVG 181 SSSATVYG P +P TE+ PLS+T+PYGR KLMVE IL D HVA P RIALLRYFNPVG Sbjct: 125 SSSATVYGDPASVPITEEFPLSATNPYGRSKLMVEQILGDLHVADPSWRIALLRYFNPVG 184 Query: 182 AHHSGLIGEDPQGVPNNLMPFVTQVAIGKRDRLQIWGGDYDTHDGTGVRDYIHVEDLAVG 241 AH SGLIGEDP G+PNNL+PF+ QVA G+R L ++G DY T DGTGVRDYIHV DLA+G Sbjct: 185 AHESGLIGEDPSGIPNNLLPFIAQVADGRRAALSVYGSDYPTPDGTGVRDYIHVVDLALG 244 Query: 242 HVKALEHLRQ-PQCFAVNLGSGRGHSVLEVVKAFEQACGKSIPYEIGPRRAGDLPAYYAD 300 H+K L L Q P + NLG+GRG+SVLE++ AFEQACG +PY++ RR GD+ YA Sbjct: 245 HLKTLRKLEQGPGVYTYNLGTGRGNSVLEMLAAFEQACGNKLPYQLVDRRPGDIACCYAA 304 Query: 301 VTLAKSLLGWKATRSLETMCADSWRWQ 327 A+ LGW+A R +E MCAD+WRWQ Sbjct: 305 TERAERELGWRAQRGIEAMCADTWRWQ 331 >gi|300313483|ref|YP_003777575.1| NAD_dependent epimerase/dehydratase [Herbaspirillum seropedicae SmR1] Length = 333 Score = 107 bits (268), Expect = 1e-24, Method: Compositional matrix adjust. Identities = 94/296 (31%), Positives = 142/296 (47%), Gaps = 26/296 (8%) Query: 3 ILVTGGAGYIGSHTCVELLKAG-HQVVVFDNFCNSFPESVRRVEKITGKTLALVEGDIRD 61 I+V GGAG IGSHT L+K +++++DNF E+++ K + V GDI Sbjct: 8 IVVIGGAGLIGSHTVDHLVKEDVKEILIYDNFLRGSRENLQNALKDPRVKIYDVGGDIMQ 67 Query: 62 REALEKALRSYECDAVIHFAGLKAVGESVAKPLEYYDNNVVGTVRLLEAMQATGVKTIVF 121 + L+ AL D V H A L + + P +D NV GT ++EA A GVK +V+ Sbjct: 68 TDILQSALEG--ADGVFHLAALWLL-QCHEYPRSAFDVNVRGTFNVMEACVAKGVKRLVY 124 Query: 122 SSSATVYGVPQFLPYTEDHPLSSTSPYGRGKLMVEDILRDYHVAQPDSRIALLRYFN--- 178 SSSA+VYG P EDHP ++ + YG K+ E +LR +H + + LRY N Sbjct: 125 SSSASVYGDAVREPMDEDHPYNNKNFYGATKIAGETMLRSFHHRYGLNYVG-LRYMNVYG 183 Query: 179 PVGAHHSGLIGEDPQGVPNNLMPFVTQVAIGKRDRLQIWGGDYDTHDGTGVRDYIHVEDL 238 P +H I +M + AI K + I G DG+ D++ VED Sbjct: 184 PRQDYHGAYIAV--------IMKMLD--AIDKGESPTIMG------DGSEAFDFVAVEDC 227 Query: 239 AVGHVKALEHLRQPQCFAVNLGSGRGHSVLEVVKAFEQACGKSIPYEIGPRRAGDL 294 + +V A++ Q + N+G+G+ S+ E+ + + G P PR L Sbjct: 228 GLANVCAMKAETADQYY--NVGTGKRTSLKELAELLLEITGCDKPITFAPRSQATL 281 >gi|300313479|ref|YP_003777571.1| UDP-galactose-4-epimerase [Herbaspirillum seropedicae SmR1] Length = 357 Score = 101 bits (251), Expect = 9e-23, Method: Compositional matrix adjust. Identities = 95/339 (28%), Positives = 151/339 (44%), Gaps = 30/339 (8%) Query: 4 LVTGGAGYIGSHTCVELLKAGHQVVVFDNFCNSFPESVRRVEKITG----KTLALVEGDI 59 LVTG AG+IGS+ LLK +VV DNF F ++ V+ + VEGDI Sbjct: 20 LVTGVAGFIGSNLLETLLKLNQRVVGLDNFSTGFQHNLDEVKALVTPEQWANFRFVEGDI 79 Query: 60 RD----REALE-KALRSYECDAVIHFAGLKAVGESVAKPLEYYDNNVVGTVRLLEAMQAT 114 R R A+ +AL S + V+H A L +V S+ P+ NN+ G + +L A + Sbjct: 80 RKLDDCRAAMHHEALGSNIVEYVLHQAALGSVPRSIEDPITTNQNNIDGFLNMLVAARDA 139 Query: 115 GVKTIVFSSSATVYGVPQFLPYTEDHPLSSTSPYGRGKLMVEDILRDYHVAQPDSRIALL 174 VK V+++S++ YG LP ED SPY K V ++ D + L Sbjct: 140 RVKRFVYAASSSTYGDHPGLPKVEDRIGKPLSPYAVTKF-VNELYADVFARTYGLQTIGL 198 Query: 175 RYFNPVGAHHSGLIGEDPQGVPNNLMPFVTQVAIGKRDRLQIWGGDYDTHDGTGVRDYIH 234 RYFN G ++P G ++P AI K +++ I G DG RD+ + Sbjct: 199 RYFNIFGRR------QNPDGAYAAVIPKWIAAAI-KGEQIYING------DGETSRDFCY 245 Query: 235 VEDLAVGHVKALEHLRQPQCFAV-NLGSGRGHSVLEVVKAFEQACGKSIPYEIGPR---- 289 V++ ++ A L+ V N+ G ++ E+ + + P+ + Sbjct: 246 VDNAVQANLLAACTLQDEAANQVYNVAVGDRTTLKELYQFLRDNLSATFPHLRDAQPIFR 305 Query: 290 --RAGDLPAYYADVTLAKSLLGWKATRSLETMCADSWRW 326 RAGD+ AD+ + LLG++ + + +S W Sbjct: 306 EFRAGDVRHSLADIGKGRRLLGYEPSHRIGRGLKESMNW 344 >gi|300312049|ref|YP_003776141.1| UDP-glucose 4-epimerase [Herbaspirillum seropedicae SmR1] Length = 347 Score = 92.0 bits (227), Expect = 6e-20, Method: Compositional matrix adjust. Identities = 92/338 (27%), Positives = 141/338 (41%), Gaps = 36/338 (10%) Query: 4 LVTGGAGYIGSHTCVELLKAGHQVVVFDNFCNSFPESVRRVEKITGK----TLALVEGDI 59 L+TG AG+IGS+ LL G QV DNF ++ V G +EGDI Sbjct: 19 LITGVAGFIGSNLLARLLTLGQQVTGLDNFSTGRQANLEEVRAEVGAAAWANFRFIEGDI 78 Query: 60 RDREALEKALRSYECDAVIHFAGLKAVGESVAKPLEYYDNNVVGTVRLLEAMQATGVKTI 119 D +A D V+H A L +V S+ P+ NNV G + +L A + GV Sbjct: 79 CDAATCLRACDG--ADYVLHQAALGSVSRSIEDPVRTNLNNVNGFLNMLVAARDAGVARF 136 Query: 120 VFSSSATVYGVPQFLPYTEDHPLSSTSPYGRGK----LMVEDILRDYHVAQPDSRIALLR 175 V+++S++ YG LP E+ SPY K L E R Y + LR Sbjct: 137 VYAASSSTYGDHPGLPKVEEVIGKPLSPYAVTKYVNELYAEVFARCYGMESIG-----LR 191 Query: 176 YFNPVGAHHSGLIGEDPQGVPNNLMP-FVTQVAIGKRDRLQIWGGDYDTHDGTGVRDYIH 234 YFN G +DP G ++P +VT A+ + L+I G DG RD+ + Sbjct: 192 YFNVFGRR------QDPHGAYAAVIPQWVT--AMIRNGELRING------DGQTSRDFCY 237 Query: 235 VEDLAVGHVKALEHLRQPQCFAVNLGSGRGHSVLEVVKAFEQACGKSIPYEIGPR----- 289 ++++ ++ A + N+ S+ ++ + + P+ R Sbjct: 238 IDNVVQANLLAATAGEEAVNQVYNVALNDRTSLNDLYAMMRELLVQRFPHLQEHRPSYVG 297 Query: 290 -RAGDLPAYYADVTLAKSLLGWKATRSLETMCADSWRW 326 RAGD+ AD+ A+ LG+ T L + W Sbjct: 298 FRAGDIRDSQADIGKARQRLGYAPTHRLRAGLEQAMSW 335 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.138 0.419 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,111,503 Number of extensions: 47887 Number of successful extensions: 163 Number of sequences better than 1.0e-08: 9 Number of HSP's gapped: 156 Number of HSP's successfully gapped: 9 Length of query: 336 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 250 Effective length of database: 1,212,660 Effective search space: 303165000 Effective search space used: 303165000 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)