BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0004.0050.p (131 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313683|ref|YP_003777775.1| dihydroneopterin aldolase [Herb... 231 2e-62 >gi|300313683|ref|YP_003777775.1| dihydroneopterin aldolase [Herbaspirillum seropedicae SmR1] Length = 131 Score = 231 bits (589), Expect = 2e-62, Method: Compositional matrix adjust. Identities = 109/128 (85%), Positives = 116/128 (90%) Query: 1 MFSLSHPELNDCRRLFLRNYEVLINIGVHEFEKKGEQRILINVDLFIPLAMSTPEHDQLQ 60 M LSHP L DCRRLFLRNYEV INIGVHEFEKKGEQRILINVDL++PLA +TP D L Sbjct: 1 MLFLSHPSLQDCRRLFLRNYEVFINIGVHEFEKKGEQRILINVDLYVPLAETTPTADHLD 60 Query: 61 EVVDYDFIRSTIAKRMAQGHIHLQETLCDDVVKAMLAHPKVRAVRVSTEKPDVYPDCDSV 120 EVVDYDFIRSTIA+RMA+GHIHLQETLCDDV+ AMLAHPKVRAVRVSTEKPDVYPDCDSV Sbjct: 61 EVVDYDFIRSTIAQRMARGHIHLQETLCDDVMTAMLAHPKVRAVRVSTEKPDVYPDCDSV 120 Query: 121 GVEVFRVK 128 GVE FR+K Sbjct: 121 GVETFRIK 128 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.324 0.139 0.408 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 356,956 Number of extensions: 11742 Number of successful extensions: 40 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 40 Number of HSP's successfully gapped: 1 Length of query: 131 Length of database: 1,619,870 Length adjustment: 75 Effective length of query: 56 Effective length of database: 1,264,745 Effective search space: 70825720 Effective search space used: 70825720 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.5 bits) S2: 125 (52.8 bits)