BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0004.0049.p (216 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313682|ref|YP_003777774.1| short-chain alcohol dehydrogena... 239 2e-64 gi|300311122|ref|YP_003775214.1| short-chain dehydrogenase [Herb... 71 7e-14 gi|300311869|ref|YP_003775961.1| short-chain alcohol dehydrogena... 64 1e-11 gi|300313009|ref|YP_003777101.1| 3-oxoacyl-(acyl-carrier-protein... 60 1e-10 gi|300313186|ref|YP_003777278.1| short-chain dehydrogenase [Herb... 59 3e-10 >gi|300313682|ref|YP_003777774.1| short-chain alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 255 Score = 239 bits (609), Expect = 2e-64, Method: Compositional matrix adjust. Identities = 124/180 (68%), Positives = 140/180 (77%) Query: 6 PSRQQVALVTGAAKRIGRTIALALARDGWDIAVHYGKSAAEAQALVAEIAALGRRAIALQ 65 PS +ALVTGAA+RIGR IALALA+ GWD+AVHY +S EA LV EI LGRRA+A+Q Sbjct: 4 PSSAGIALVTGAARRIGRHIALALAQSGWDVAVHYNRSREEADTLVREIETLGRRALAVQ 63 Query: 66 CELADEAGVRTLVRQAGRLGPVACVVNNASLFREDRAESFSMDALDAHMRANVAAPVLLA 125 EL DE VR+L+ QA LG ++CVVNNASLF D A SF + L HM ANV APVLLA Sbjct: 64 AELGDETQVRSLLPQAAPLGRISCVVNNASLFDYDDAASFGNERLLRHMSANVGAPVLLA 123 Query: 126 QALYDATPPGRQAVVVNLLDQKLYNPNPDFLSYTLSKAALQSATTLLAQALANIXRTTSL 185 QALY+ATP G+QAVV+NLLDQKLYNPNPDFLSYTLSKAAL SATTLLAQALA R + Sbjct: 124 QALYEATPQGQQAVVINLLDQKLYNPNPDFLSYTLSKAALHSATTLLAQALAPKVRVVGV 183 >gi|300311122|ref|YP_003775214.1| short-chain dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 251 Score = 71.2 bits (173), Expect = 7e-14, Method: Compositional matrix adjust. Identities = 60/176 (34%), Positives = 92/176 (52%), Gaps = 8/176 (4%) Query: 9 QQVALVTGAAKRIGRTIALALARDGWDIAVHYGKSAAEAQALVAEIAALGRRAIALQCEL 68 +V LVTG ++ IG AL + G+ +AV+Y + A A A+VA+I A G RAIA+ ++ Sbjct: 5 DKVILVTGGSRGIGAATALLAGQRGYAVAVNYVTNRAAADAVVAQIQAAGGRAIAVGGDV 64 Query: 69 ADEAGVRTLVRQA-GRLGPVACVVNNASLF-REDRAESFSMDALDAHMRANVAAPVLLAQ 126 A E V L G LG V +VNNA + + R E S + ++ + ANV +L A+ Sbjct: 65 AREEDVLQLFATVDGELGRVTALVNNAGVLDHQSRLEDMSAERINRVLIANVTGSLLCAR 124 Query: 127 AL---YDATPPGRQAVVVNL--LDQKLYNPNPDFLSYTLSKAALQSATTLLAQALA 177 G+ +VNL + KL P +++ Y SK A+ + T LA+ +A Sbjct: 125 EAVKRMSTRHGGQGGAIVNLSSMAAKLGGPG-EYVDYAASKGAIDAFTIGLAKEVA 179 >gi|300311869|ref|YP_003775961.1| short-chain alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 257 Score = 63.5 bits (153), Expect = 1e-11, Method: Compositional matrix adjust. Identities = 49/175 (28%), Positives = 81/175 (46%), Gaps = 9/175 (5%) Query: 10 QVALVTGAAKRIGRTIALALARDGWDIAVHYGKSAAEAQALVAEIAALGRRAIALQCELA 69 ++AL+TG ++ +G+ AL LARDG + + Y + A+A VAE+ ALG A AL+ +LA Sbjct: 9 RIALITGGSRGLGKNTALHLARDGAGVILTYVGNQQAAEATVAELQALGAVAAALRLDLA 68 Query: 70 DEA-------GVRTLVRQAGRLGPVACVVNNASLFREDRAESFSMDALDAHMRANVAAPV 122 D A VR ++ + +VNN + E S DA + ++ P Sbjct: 69 DSASFPAFAQAVREALKTHWQAEHFHYLVNNGGMGMHASIEETSEAVFDALFKVHLKGPF 128 Query: 123 LLAQALYDATPPGRQAVVVNLLDQKLYNPNPDFLSYTLSKAALQSATTLLAQALA 177 L QAL G + ++N+ P + +Y K ++ T +A+ Sbjct: 129 FLTQALLPLLAQGGR--IINISSGLARFSLPGYAAYASMKGGVEVMTRYMAKEFG 181 >gi|300313009|ref|YP_003777101.1| 3-oxoacyl-(acyl-carrier-protein) reductase [Herbaspirillum seropedicae SmR1] Length = 245 Score = 60.5 bits (145), Expect = 1e-10, Method: Compositional matrix adjust. Identities = 55/178 (30%), Positives = 84/178 (47%), Gaps = 22/178 (12%) Query: 13 LVTGAAKRIGRTIALALARDGWDIAVHYGKSAAEAQALVAEIAALGRRAIALQCELAD-E 71 VTG+++ IGR IAL LAR+G+DI +H A + AEIAALGR+ LQ ++A+ E Sbjct: 10 FVTGSSRGIGRAIALRLAREGYDIVLHCHARRDAADQVAAEIAALGRQVRVLQFDVANRE 69 Query: 72 AGVRTLVRQAGRLGPVACVVNNASLFREDRAESFSMDALDAHMRANVAAPVLLAQALYDA 131 + L+ + G VV NA + R++ + + D+ +R N+ Y+ Sbjct: 70 QAAQVLLADVEQHGCYYGVVCNAGITRDNAFPAMPGEDWDSVLRTNL-------DGFYNV 122 Query: 132 TPP-----------GRQAVVVNLLDQKLYNPNPDFLSYTLSKAALQSATTLLAQALAN 178 P GR +V L N ++Y+ +KA + AT LA LA Sbjct: 123 LNPLVMPMVRRRAAGR---IVTLASVSGLIGNRGQVNYSAAKAGVIGATKALALELAK 177 >gi|300313186|ref|YP_003777278.1| short-chain dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 257 Score = 58.9 bits (141), Expect = 3e-10, Method: Compositional matrix adjust. Identities = 39/110 (35%), Positives = 60/110 (54%), Gaps = 1/110 (0%) Query: 10 QVALVTGAAKRIGRTIALALARDGWDIAVHYGKSAAEAQALVAEIAALGRRAIALQCELA 69 +VA V G ++ IG I LA +G +A Y S +AQALV+ + A G RA+A+Q + A Sbjct: 18 KVAFVQGGSRGIGAAIVQRLAAEGATVAFTYVSSPDKAQALVSSVQAKGGRALAIQADSA 77 Query: 70 DEAGVRTLVRQAGR-LGPVACVVNNASLFREDRAESFSMDALDAHMRANV 118 D A ++ +R A G + +VNNA + + F ++ LD + NV Sbjct: 78 DAAALQQAIRLAAETFGRLDILVNNAGVLAMGPLDQFKLEDLDRTLAVNV 127 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.128 0.371 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 452,242 Number of extensions: 15402 Number of successful extensions: 121 Number of sequences better than 1.0e-08: 10 Number of HSP's gapped: 126 Number of HSP's successfully gapped: 10 Length of query: 216 Length of database: 1,619,870 Length adjustment: 81 Effective length of query: 135 Effective length of database: 1,236,335 Effective search space: 166905225 Effective search space used: 166905225 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 128 (53.9 bits)