BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0004.0045.p (50 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313680|ref|YP_003777772.1| two component response regulato... 98 2e-22 gi|300311750|ref|YP_003775842.1| transcription response regulato... 69 8e-14 >gi|300313680|ref|YP_003777772.1| two component response regulator protein [Herbaspirillum seropedicae SmR1] Length = 218 Score = 97.8 bits (242), Expect = 2e-22, Method: Compositional matrix adjust. Identities = 43/49 (87%), Positives = 48/49 (97%) Query: 1 LSRAQLEEKLYGWDDGVDSNAIEVHIHALRKKLGSDFIKNVRGIGYLVP 49 LSRAQLEEKLYGWDD ++SNA+EVHIHALRKK+GSDFIKNVRG+GYLVP Sbjct: 169 LSRAQLEEKLYGWDDSIESNAVEVHIHALRKKVGSDFIKNVRGVGYLVP 217 >gi|300311750|ref|YP_003775842.1| transcription response regulator protein [Herbaspirillum seropedicae SmR1] Length = 220 Score = 68.9 bits (167), Expect = 8e-14, Method: Compositional matrix adjust. Identities = 32/50 (64%), Positives = 42/50 (84%) Query: 1 LSRAQLEEKLYGWDDGVDSNAIEVHIHALRKKLGSDFIKNVRGIGYLVPK 50 LSRA+LEEKLYGW + V+SNA+E IH+LR+KL + IKNVRG+G+LV + Sbjct: 169 LSRAELEEKLYGWGEEVESNAVEFLIHSLRRKLDKEAIKNVRGMGWLVQR 218 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.140 0.417 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 150,319 Number of extensions: 4117 Number of successful extensions: 29 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 29 Number of HSP's successfully gapped: 2 Length of query: 50 Length of database: 1,619,870 Length adjustment: 23 Effective length of query: 27 Effective length of database: 1,510,965 Effective search space: 40796055 Effective search space used: 40796055 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 123 (52.0 bits)