BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0004.0040.p
         (410 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300310601|ref|YP_003774693.1| citrate synthase [Herbaspirillu...   280   1e-76
gi|300312419|ref|YP_003776511.1| methylcitrate synthase [Herbasp...    90   3e-19
gi|300312274|ref|YP_003776366.1| citrate synthase [Herbaspirillu...    75   9e-15

>gi|300310601|ref|YP_003774693.1| citrate synthase [Herbaspirillum
           seropedicae SmR1]
          Length = 431

 Score =  280 bits (716), Expect = 1e-76,   Method: Compositional matrix adjust.
 Identities = 143/219 (65%), Positives = 167/219 (76%), Gaps = 3/219 (1%)

Query: 110 AEGSGALMRLLAASLLGTRPSPAPIHEQCAKAWKLDAHGAALVRMALVLCADHELNASSF 169
           A+G G L+R+LAA LL TRP  +PIH QCA+AW +   G  L+RMALVLCADHELNASSF
Sbjct: 171 AQGCGDLVRILAACLLRTRPDTSPIHVQCARAWGVGRAGGELIRMALVLCADHELNASSF 230

Query: 170 TARCVASTGTSLRASVIAGLAALTGGKHGGATARVEALLDEIDANGKADIAAGLQRRLAR 229
           T+RCVAST  SLRA VIAGLA L+GG+HG  T RVEAL DE+   G  D+   ++ RL+R
Sbjct: 231 TSRCVASTDASLRAVVIAGLAGLSGGRHGATTGRVEALWDEL---GTRDVDRKMRERLSR 287

Query: 230 GDDLPGFGHHLYPDGDVRANALLAEVLPHHPAWRDIIAAARNLIGQHPSLDFALVALRRH 289
           GDDLPGFGH LYPDGD RA+ LL+++LP H  W  +I A   L GQ PS+DFALVALRRH
Sbjct: 288 GDDLPGFGHTLYPDGDPRASYLLSQILPRHSPWAAMIDAGITLTGQKPSVDFALVALRRH 347

Query: 290 LQLPVGAAFGLFALGRGMGWIAQALEQRASGELIRPXAL 328
           L+LPVGAAFGLFALGR +GWIA  LEQR    LIRP A+
Sbjct: 348 LRLPVGAAFGLFALGRSIGWIAHGLEQREQHHLIRPRAV 386


>gi|300312419|ref|YP_003776511.1| methylcitrate synthase
           [Herbaspirillum seropedicae SmR1]
          Length = 384

 Score = 90.1 bits (222), Expect = 3e-19,   Method: Compositional matrix adjust.
 Identities = 60/202 (29%), Positives = 97/202 (48%), Gaps = 19/202 (9%)

Query: 136 EQCAKAWKLDAHGAALVRMALVLCADHELNASSFTARCVASTGTSLRASVIAGLAALTGG 195
           E+ ++AW+   H       +L+L A+HE NAS+FT R +A TG+ + +++   + AL G 
Sbjct: 175 EKPSEAWEKAMH------TSLILYAEHEFNASTFTGRVIAGTGSDMYSAITGAIGALRGP 228

Query: 196 KHGGATARVEALLDEIDANGKADIAAGLQRRLARGDDLPGFGHHLYPDGDVRANALLAEV 255
           KHGGA      +    D   +A+  A ++RR+   + + GFGH +Y   D R N ++ EV
Sbjct: 229 KHGGANEVAFEIQKRYDNPDEAE--ADIRRRVENKEVVIGFGHPVYTISDPR-NKVIKEV 285

Query: 256 ---LPHHPAWRDIIAAAR-------NLIGQHPSLDFALVALRRHLQLPVGAAFGLFALGR 305
              L        +   A        ++    P+LD+        + +P      LF + R
Sbjct: 286 ARKLSKEAGSTKMFDIAERLETVMWDIKKMFPNLDWFSAVSYHMMGVPTAMFTPLFVIAR 345

Query: 306 GMGWIAQALEQRASGELIRPXA 327
             GW A  +EQR   ++IRP A
Sbjct: 346 TSGWAAHIIEQRIDNKIIRPSA 367


>gi|300312274|ref|YP_003776366.1| citrate synthase [Herbaspirillum
           seropedicae SmR1]
          Length = 456

 Score = 75.1 bits (183), Expect = 9e-15,   Method: Compositional matrix adjust.
 Identities = 59/185 (31%), Positives = 86/185 (46%), Gaps = 22/185 (11%)

Query: 156 LVLCADHELNASSFTARCVASTGTSLRASVIAGLAALTGGKHGGATARVEALLDEIDANG 215
           L+L ADHE NAS+ T R   S+G +  A + AG+A L G  HGGA      +L++I +  
Sbjct: 251 LILHADHEQNASTSTVRLAGSSGANPFACIAAGIACLWGPAHGGANEAALNMLEQIGSVD 310

Query: 216 KADIAAGLQRRLARGDDLPGFGHHLYPDGDVRANAL---LAEVLPH-----HPAWR---- 263
           K        +    G  L GFGH +Y + D RA  +     EVL        P ++    
Sbjct: 311 KIPEFIKQVKDKNSGVKLMGFGHRVYKNYDPRAKLMRETCYEVLNELGLHDDPLFKLAME 370

Query: 264 -------DIIAAARNLIGQHPSLDFALVALRRHLQLPVGAAFGLFALGRGMGWIAQALEQ 316
                  D    +R L   +P++DF    ++R L +P      +FA+ R +GWIAQ  E 
Sbjct: 371 LEKIALEDEYFVSRKL---YPNVDFYSGIVQRALGIPTSMFTCIFAMARTIGWIAQWNEM 427

Query: 317 RASGE 321
            +  E
Sbjct: 428 ISDPE 432


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.323    0.136    0.407 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 1,322,414
Number of extensions: 57519
Number of successful extensions: 211
Number of sequences better than 1.0e-08: 3
Number of HSP's gapped: 207
Number of HSP's successfully gapped: 3
Length of query: 410
Length of database: 1,619,870
Length adjustment: 87
Effective length of query: 323
Effective length of database: 1,207,925
Effective search space: 390159775
Effective search space used: 390159775
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.5 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (22.0 bits)
S2: 132 (55.5 bits)