BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0004.0035.p (231 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312356|ref|YP_003776448.1| cobalt/zinc/cadmium resistance ... 274 4e-75 gi|300309361|ref|YP_003773453.1| response regulator for cobalt/z... 273 8e-75 gi|300311799|ref|YP_003775891.1| two component heavy metal respo... 254 3e-69 gi|300313818|ref|YP_003777910.1| two component transcription reg... 157 1e-39 gi|300313680|ref|YP_003777772.1| two component response regulato... 149 1e-37 >gi|300312356|ref|YP_003776448.1| cobalt/zinc/cadmium resistance two component response regulator protein [Herbaspirillum seropedicae SmR1] Length = 223 Score = 274 bits (701), Expect = 4e-75, Method: Compositional matrix adjust. Identities = 132/221 (59%), Positives = 178/221 (80%), Gaps = 2/221 (0%) Query: 1 MKLLVVEDEPKTGEYIQRGLSEAGFIVDLAKNGLDGYHLAMTEPYDLIVLDVMLPDVDGW 60 M +LV+ED+PKTG+Y+++GL E+G+ VDLA+NG DG H+A+ + YDL+VLDVMLP +DGW Sbjct: 1 MAILVIEDDPKTGDYLRKGLRESGYAVDLARNGADGLHMALEQDYDLVVLDVMLPGLDGW 60 Query: 61 RIVQSLREAQKTVPVLFLTARDSVSDRVKGLEMGADDYLVKPFAFSELLARVRTLLRRGS 120 +++ +LR +++ +PVLFLTARD V DR++GL++GADDYLVKPF+F+EL+ R+RTLLRRG Sbjct: 61 QVMSALR-SKRDLPVLFLTARDHVDDRIRGLQLGADDYLVKPFSFTELVLRIRTLLRRGV 119 Query: 121 SAVLQDRLTVADLVLDLPRRTASRGGRKLVLTNKEFALLEFLARRRGEVLPRSLIASQIW 180 + + +ADL LD R +R G + LTNKEF LL L +R+GE L RS+IASQIW Sbjct: 120 TREAE-VYEIADLQLDHVRHKVTRQGVNIALTNKEFMLLHLLIKRQGEALSRSVIASQIW 178 Query: 181 DINFNSDTNVIDVAIRRLRAKVDDGFDEKLILTVRGVGYIL 221 D+NF+SDTNV+DVAI+RLRAK+D FD+KLI TVR VGY+ Sbjct: 179 DMNFDSDTNVVDVAIKRLRAKIDAPFDKKLIHTVRSVGYMF 219 >gi|300309361|ref|YP_003773453.1| response regulator for cobalt/zinc/cadmium resistance transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 235 Score = 273 bits (698), Expect = 8e-75, Method: Compositional matrix adjust. Identities = 138/221 (62%), Positives = 177/221 (80%), Gaps = 2/221 (0%) Query: 1 MKLLVVEDEPKTGEYIQRGLSEAGFIVDLAKNGLDGYHLAMTEPYDLIVLDVMLPDVDGW 60 M +L+VEDEPK+G+Y++RGL+E+GF+VD + G+DG H A T Y LI+LDVMLP ++GW Sbjct: 1 MHILLVEDEPKSGDYLRRGLTESGFVVDWVQTGVDGLHNARTLDYKLIILDVMLPGMNGW 60 Query: 61 RIVQSLREAQKTVPVLFLTARDSVSDRVKGLEMGADDYLVKPFAFSELLARVRTLLRRGS 120 I++ LR T PVLFLTARD V DRVKGLE+GADDYLVKPFA++ELLARVRTLLRRG Sbjct: 61 DILRELRRTHDT-PVLFLTARDEVEDRVKGLELGADDYLVKPFAYTELLARVRTLLRRGP 119 Query: 121 SAVLQDRLTVADLVLDLPRRTASRGGRKLVLTNKEFALLEFLARRRGEVLPRSLIASQIW 180 S + D + D+VLD+ RR +R G+++ LT KEFALL L RR GEVL R IASQ+W Sbjct: 120 SREV-DLIEYDDVVLDVLRRKVTRAGQRIDLTAKEFALLHMLIRRPGEVLSRPQIASQVW 178 Query: 181 DINFNSDTNVIDVAIRRLRAKVDDGFDEKLILTVRGVGYIL 221 D+NF+SDTNV+DVA+RRLRAK+D+GF++KL+ TV G+GY+ Sbjct: 179 DMNFDSDTNVVDVAVRRLRAKLDEGFEKKLLHTVWGMGYVF 219 >gi|300311799|ref|YP_003775891.1| two component heavy metal response transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 230 Score = 254 bits (650), Expect = 3e-69, Method: Compositional matrix adjust. Identities = 129/231 (55%), Positives = 178/231 (77%), Gaps = 3/231 (1%) Query: 1 MKLLVVEDEPKTGEYIQRGLSEAGFIVDLAKNGLDGYHLAMTEPYDLIVLDVMLPDVDGW 60 MK+L+VEDE K EY+ +GL+E G VDLA NG+DG HLA+ YD+IVLDVMLP +DG+ Sbjct: 1 MKILIVEDEQKMSEYLCKGLTEQGCAVDLAANGVDGQHLAIEHDYDVIVLDVMLPGLDGF 60 Query: 61 RIVQSLREAQKTVPVLFLTARDSVSDRVKGLEMGADDYLVKPFAFSELLARVRTLLRRGS 120 ++++LR+ ++T PV+ LTARDSV DR+KGL GADDYL+KPF+F ELLAR++ L RRG Sbjct: 61 SVLRALRKVKET-PVIMLTARDSVEDRIKGLHDGADDYLIKPFSFLELLARLQALTRRGR 119 Query: 121 SAVLQDRLTVADLVLDLPRRTASRGGRKLVLTNKEFALLEFLARRRGEVLPRSLIASQIW 180 A +L + DL +DL R A RGG ++ LT KEFALL +ARR+GE+L ++ IA +W Sbjct: 120 -AQEPAQLRIGDLQIDLLSRKAFRGGLRIDLTAKEFALLAVMARRQGEILSKTAIAELVW 178 Query: 181 DINFNSDTNVIDVAIRRLRAKVDDGFDEKLILTVRGVGYILDVPEHTESAT 231 D+NF+S+TNV++VAI+RLRAK+D F++KL+ TVRG+GY+L+ P T ++ Sbjct: 179 DMNFDSNTNVVEVAIKRLRAKIDAPFEQKLLHTVRGMGYVLE-PRATADSS 228 >gi|300313818|ref|YP_003777910.1| two component transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 224 Score = 157 bits (396), Expect = 1e-39, Method: Compositional matrix adjust. Identities = 88/224 (39%), Positives = 134/224 (59%), Gaps = 6/224 (2%) Query: 1 MKLLVVEDEPKTGEYIQRGLSEAGFIVDLAKNGLDGYHLAMTEPYDLIVLDVMLPDVDGW 60 M++L+VED + ++ + + +A V+ A NG D L +T+ Y L++LD+ LP +DG Sbjct: 1 MRILLVEDHIELSRWLAKAMRDAHLTVECAHNGADADSLLLTQDYALVILDLTLPRMDGM 60 Query: 61 RIVQSLREAQKTVPVLFLTARDSVSDRVKGLEMGADDYLVKPFAFSELLARVRTLLRRGS 120 +++ +R PVL LTAR ++DRV GL MGADDYL KPF EL ARV+ LLRR Sbjct: 61 EVLKRMRARGNRTPVLILTARGGLADRVSGLNMGADDYLAKPFELEELEARVKALLRRSQ 120 Query: 121 SAVLQDRLTVA--DLVLDLPRRTASRGGRKLVLTNKEFALLEFLARRRGEVLPRSLIASQ 178 + + +TV+ L D RT + GG L LT +E A+LE L R G +P+ + Q Sbjct: 121 N---NEAVTVSCGALSFDTVSRTFTYGGELLALTPREHAVLEALIVRHGHTVPKEKLFQQ 177 Query: 179 IWDINFNSDTNVIDVAIRRLRAKVD-DGFDEKLILTVRGVGYIL 221 ++ + N+ + I++ I RLR K++ +G I T+RG+GY+L Sbjct: 178 VFTLEDNASMDAIEIYIHRLRKKLEHEGPGRVGITTLRGLGYLL 221 >gi|300313680|ref|YP_003777772.1| two component response regulator protein [Herbaspirillum seropedicae SmR1] Length = 218 Score = 149 bits (377), Expect = 1e-37, Method: Compositional matrix adjust. Identities = 84/221 (38%), Positives = 135/221 (61%), Gaps = 5/221 (2%) Query: 1 MKLLVVEDEPKTGEYIQRGLSEAGFIVDLAKNGLDGYHLAMTEPYDLIVLDVMLPDVDGW 60 M++L+VED+P GE +++GL + GF +D ++G TE Y +++LD+ LP DG Sbjct: 1 MRVLLVEDDPMVGEAVRKGLRQDGFAIDWVQDGKSADVALRTEDYAMLLLDLGLPQKDGL 60 Query: 61 RIVQSLREAQKTVPVLFLTARDSVSDRVKGLEMGADDYLVKPFAFSELLARVRTLLRRGS 120 ++++LRE +PVL TARD+V+DRV GL+ GADDYL+KPF EL AR+R L RR + Sbjct: 61 AVLRTLRERGNAIPVLITTARDAVADRVAGLDAGADDYLIKPFDLEELSARMRALSRRQA 120 Query: 121 SAVLQDRLTVADLVLDLPRRTASRGGRKLVLTNKEFALLEFLARRRGEVLPRSLIASQIW 180 + + V +++L+ + G+ + L+ +EF LL+ R G VL R+ + +++ Sbjct: 121 GRA-ESLVQVREVILNPATHEVTVSGKPVNLSAREFTLLQAFMDRPGVVLSRAQLEEKLY 179 Query: 181 DINFNSDTNVIDVAIRRLRAKVDDGFDEKLILTVRGVGYIL 221 + + ++N ++V I LR KV F I VRGVGY++ Sbjct: 180 GWDDSIESNAVEVHIHALRKKVGSDF----IKNVRGVGYLV 216 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.140 0.394 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 682,395 Number of extensions: 26937 Number of successful extensions: 255 Number of sequences better than 1.0e-08: 41 Number of HSP's gapped: 215 Number of HSP's successfully gapped: 41 Length of query: 231 Length of database: 1,619,870 Length adjustment: 82 Effective length of query: 149 Effective length of database: 1,231,600 Effective search space: 183508400 Effective search space used: 183508400 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 129 (54.3 bits)